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Interacções de proteínas do plasma com potenciais fármacos: estudos 3D a nível molecular

Santos, Marino Filipe Alves dos
Fonte: Faculdade de Ciências e Tecnologia Publicador: Faculdade de Ciências e Tecnologia
Tipo: Dissertação de Mestrado
Publicado em //2011 POR
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Dissertação para obtenção do Grau de Mestre em Biotecnologia; Outrora considerado um produto tóxico, reconhecem-se, hoje, ao monóxido de carbono (CO) efeitos biológicos e terapêuticos (nomeadamente anti-inflamatórios); neste sentido, desenvolveram-se metodologias para a sua aplicação destacando-se as Moléculas Libertadoras de Monóxido de Carbono (CORMs). Perspectivando a passagem a fármacos, é indispensável elucidar os mecanismos de acção e de transporte no organismo que dependem, significativamente, das interacções estabelecidas com proteínas sanguíneas (hemoglobina, albumina e transferrina). Assim, no presente trabalho, empregaram-se técnicas de Cristalografia de Raios-X e de Espectroscopia (Ultra-Violeta/Visível e Infra-vermelhos) para caracterizar interacções entre as referidas proteínas (juntamente com lisozima) e possíveis CORMs desenvolvidos pela empresa Alfama. Os estudos com a lisozima indicam a formação de aductos com o ALF 850 confirmando-se, por Cristalografia, a ligação do composto à Histidina 15 com a libertação de 2 moléculas de CO e comprovando-se o respectivo efeito terapêutico. Analogamente, conseguiram-se dados indicativos da formação de aductos entre a hemoglobina e o ALF 850...

Gladiolus development in response to bulb treatment with different concentrations of humic acids

Baldotto,Marihus Altoé; Baldotto,Lílian Estrela Borges
Fonte: Universidade Federal de Viçosa Publicador: Universidade Federal de Viçosa
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2013 EN
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Gladiolus is an ornamental species produced for cut flowers and propagated by corms. The early flowering and increase in the number of flower buds, besides the production of commercial corms are constant challenges to be addressed in the crop improvement. Commercial production of ornamentals is technologically accelerated by means of growth regulators. Among them, the auxins stand out for their key role in the adventitious rooting and cell elongation. Alternatively, the humic substances present in the organic matter also have biostimulating effect, which is very similar to the auxinic effect. Therefore, this work aimed to study the growth and development of gladiolus in response to application of different concentrations of humic acids (HA) isolated from vermicompost. Corms were soaked for 24 hours in solutions containing 0, 10, 20, 30 and 40 mmol L-1 of C from HA. The corms were planted in 10-dm³ plastic bags filled with substrate and kept in a greenhouse. Growth of shoots and roots was evaluated. The results showed that the use of HA accelerates growth, and anticipates and increases flowering of Gladiolus.

Anatomical alterations in taro corms with "metsubure" symptoms

Pereira,Francisco HF; Puiatti,Mário; Meira,Renata MSA; Fontes,Paulo CR
Fonte: Associação Brasileira de Horticultura Publicador: Associação Brasileira de Horticultura
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2012 EN
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Taro plants present high Ca absorption. There is evidence that the disorder "metsubure" is of nutritional origin being attributed to calcium deficiency. The metsubure is characterized by the corm apical bud suppression and a smooth lesion in the apex. This disorder reduces the taro corms value as food and hinders their utilization as a propagative material in commercial fields. In subterranean structures, Ca deficiency is believed to be more harmful to the vascular tissue cells since they have thicker walls than other tissue cells. Thus, this work aimed to characterize the anatomical changes in taro corms (Colocasia esculenta), cv. Chinese (BGH 5928) with "metsubure" symptoms. For this, corms with and without this symptom were obtained from plants grown in a greenhouse, in pots, containing soil. Corms presenting "metsubure" showed anatomical changes with damage in the vascular system which led to the suppression of apical bud formation, with detection of a wound periderm. In these corms, the periderm was thicker with compact and suberized cell layers; moreover parenchyma cells were larger with less starch accumulation as compared to corms without "metsubure" symptoms.

Determination of some phenolic compounds in Crocus sativus L. corms and its antioxidant activities study

Esmaeili, N; Ebrahimzadeh, H; Abdi, K; Safarian, S
Fonte: Medknow Publications Publicador: Medknow Publications
Tipo: Artigo de Revista Científica
Publicado em //2011 EN
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It is well known that phenolic compounds are constituents of many plants. In this study, the total phenolics content in Crocus sativus L. corms in dormancy and waking stages were determined by the Folin-Ciocalteu method. Analysis was carried out by gas chromatography-mass spectrometry (GC-MS) after silylation by N-methyl-N-trimethylsilyl trifluroacetamide (MSTFA) + %1 trimethyl iodosilane (TMIS). Numerous compounds were detected and 11 compounds were identified. The highest phenolics content in waking corms was observed for gentisic acid (5.693 ± 0.057 μg/g) and the lowest for gallic acid (0.416 ± 0.006 μg/g); also these two phenolic compounds are the highest (0.929 ± 0.015 μg/g) and lowest (0.017 ± 0.001 μg/g) phenolics in dormant corms, respectively. The results from quantization and GC-MS analysis showed a high concentration of phenolic compounds in waking corms than the dormant stage. Furthermore, the radical scavenging activities of saffron corms were studied by 1,1-diphenyl-2-pycrylhydrazyl (DPPH) test and EC 50values were determined about 2055 ppm and 8274 ppm for waking and dormant corms, respectively.

Designed Iron Carbonyls as Carbon Monoxide (CO) Releasing Molecules (CORMs): Rapid CO Release and Delivery to Myoglobin in Aqueous Buffer, and Vasorelaxation of Mouse Aorta

Gonzalez, Margarita A.; Fry, Nicole L.; Burt, Richard; Davda, Riddhi; Hobbs, Adrian; Mascharak, Pradip K.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
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The physiological roles of CO in neurotransmission, vasorelaxation and cytoprotective activities have raised interest in the design and syntheses of CO-releasing materials (CORMs) that could be employed to modulate such biological pathways. Three iron-based CORMs namely, [(PaPy3)Fe(CO)](ClO4) (1), [(SBPy3)Fe(CO)](BF4)2 (2) and [(Tpmen)Fe(CO)](ClO4)2 (3), derived from designed polypyridyl ligands have been synthesized and characterized by spectroscopy and X-ray crystallography. In these three Fe(II) carbonyls, the CO is trans to a carboxamido-N (in 1), an imine-N (in 2) and a tertiary amine-N (in 3) respectively. This structural feature has been correlated to the strength of the Fe-CO bond. The CO-releasing properties of all three carbonyls have been studied in various solvents under different experimental conditions. Rapid release of CO is observed with 2 and 3 upon dissolution in both aqueous and nonaqueous media in the presence and absence of dioxygen. With 1, CO release is observed only under aerobic conditions and the final product is an oxo-bridged diiron species while with 2 and 3, the solvent bound [(L)Fe(CO)]2+ (where L = SBPy3 or Tpmen) results upon loss of CO under both aerobic and anaerobic conditions. The apparent rates of CO loss by these CORMs are comparable to other CORMs such as [Ru(glycine)(CO)3Cl] reported recently. Facile delivery of CO to reduced myoglobin has been observed with both 2 and 3. In tissue bath experiments...

THE PHARMACOGNOSY OF NATTU ATIVIDAYAM – THE CORMS OF CRYPTOCORYNE SPIRALIS FISCH

Anandakumar, A.; Rajendran, V.; Thirugnanasambantham, M.P.; Balasubramanian, M.; Muralidharan, R.
Fonte: Medknow Publications & Media Pvt Ltd Publicador: Medknow Publications & Media Pvt Ltd
Tipo: Artigo de Revista Científica
Publicado em //1982 EN
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27.29%
The pharmacognosy of Nattu Attivdayam the corms of Cryptocoryne spiralis Fisch – its macroscopical, microscopical and chemical studies – is reported

Determination of Metal Content in Crocus sativus L. Corms in Dormancy and Waking Stages

Esmaeili, Nardana; Ebrahimzadeh, Hassan; Abdi, Khosrou; Mirmasoumi, Masoud; Lamei, Navid; Azizi Shamami, Mehrdad
Fonte: Shaheed Beheshti University of Medical Sciences Publicador: Shaheed Beheshti University of Medical Sciences
Tipo: Artigo de Revista Científica
Publicado em //2013 EN
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More than 30 mineral elements have been found with different key functions in helping plants and animals to survive and live healthy. As a direct result, they have always attracted the attention of scientists. The quest is to find some efficient analytical and quantitative procedures in this study to determine some mineral and trace elements of Iranian Crocus sativus L. corms. Several studies have been made using distinct methods and eventually, to achieve this purpose, three analytical methods were used as follows: Neutron Activation Analysis (NAA), Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) and Atomic Absorption Spectrophotometry (AAS). Seventeen mineral and trace elements (Mg, Na, Ca, K, Mn, Zn, Cu, Pb, Hg, Ni, Fe, Co, Cd, Sr, Rb, Sc, and Br) were determined in Crocus sativus L. corms in two different physiological stages.

The Bactericidal Activity of Carbon Monoxide–Releasing Molecules against Helicobacter pylori

Tavares, Ana F.; Parente, Margarida R.; Justino, Marta C.; Oleastro, Mónica; Nobre, Lígia S.; Saraiva, Lígia M.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 26/12/2013 EN
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Helicobacter pylori is a pathogen that establishes long life infections responsible for chronic gastric ulcer diseases and a proved risk factor for gastric carcinoma. The therapeutic properties of carbon-monoxide releasing molecules (CORMs) led us to investigate their effect on H. pylori. We show that H. pylori 26695 is susceptible to two widely used CORMs, namely CORM-2 and CORM-3. Also, several H. pylori clinical isolates were killed by CORM-2, including those resistant to metronidazole. Moreover, sub-lethal doses of CORM-2 combined with metronidazole, amoxicillin and clarithromycin was found to potentiate the effect of the antibiotics. We further demonstrate that the mechanisms underpinning the antimicrobial effect of CORMs involve the inhibition of H. pylori respiration and urease activity. In vivo studies done in key cells of the innate immune system, such as macrophages, showed that CORM-2, either alone or when combined with metronidazole, strongly reduces the ability of H. pylori to infect animal cells. Hence, CORMs have the potential to kill antibiotic resistant strains of H. pylori.

Different design of enzyme-triggered CO-releasing molecules (ET-CORMs) reveals quantitative differences in biological activities in terms of toxicity and inflammation

Stamellou, E.; Storz, D.; Botov, S.; Ntasis, E.; Wedel, J.; Sollazzo, S.; Krämer, B.K.; van Son, W.; Seelen, M.; Schmalz, H.G.; Schmidt, A.; Hafner, M.; Yard, B.A.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em 05/06/2014 EN
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Acyloxydiene–Fe(CO)3 complexes can act as enzyme-triggered CO-releasing molecules (ET-CORMs). Their biological activity strongly depends on the mother compound from which they are derived, i.e. cyclohexenone or cyclohexanedione, and on the position of the ester functionality they harbour. The present study addresses if the latter characteristic affects CO release, if cytotoxicity of ET-CORMs is mediated through iron release or inhibition of cell respiration and to what extent cyclohexenone and cyclohexanedione derived ET-CORMs differ in their ability to counteract TNF-α mediated inflammation. Irrespective of the formulation (DMSO or cyclodextrin), toxicity in HUVEC was significantly higher for ET-CORMs bearing the ester functionality at the outer (rac-4), as compared to the inner (rac-1) position of the cyclohexenone moiety. This was paralleled by an increased CO release from the former ET-CORM. Toxicity was not mediated via iron as EC50 values for rac-4 were significantly lower than for FeCl2 or FeCl3 and were not influenced by iron chelation. ATP depletion preceded toxicity suggesting impaired cell respiration as putative cause for cell death. In long-term HUVEC cultures inhibition of VCAM-1 expression by rac-1 waned in time, while for the cyclohexanedione derived rac-8 inhibition seems to increase. NFκB was inhibited by both rac-1 and rac-8 independent of IκBα degradation. Both ET-CORMs activated Nrf-2 and consequently induced the expression of HO-1.

Carbon Monoxide Induces Heme Oxygenase-1 to Modulate STAT3 Activation in Endothelial Cells via S-Glutathionylation

Yang, Yan-Chang; Huang, Yu-Ting; Hsieh, Chia-Wen; Yang, Po-Min; Wung, Being-Sun
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 29/07/2014 EN
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IL-6/STAT3 pathway is involved in a variety of biological responses, including cell proliferation, differentiation, apoptosis, and inflammation. In our present study, we found that CO releasing molecules (CORMs) suppress IL-6-induced STAT3 phosphorylation, nuclear translocation and transactivity in endothelial cells (ECs). CO is a byproduct of heme degradation mediated by heme oxygenase (HO-1). However, CORMs can induce HO-1 expression and then inhibit STAT3 phosphorylation. CO has been found to increase a low level ROS and which may induce protein glutathionylation. We hypothesized that CORMs increases protein glutathionylation and inhibits STAT3 activation. We found that CORMs increase the intracellular GSSG level and induce the glutathionylation of multiple proteins including STAT3. GSSG can inhibit STAT3 phosphorylation and increase STAT3 glutathionylation whereas the antioxidant enzyme catalase can suppress the glutathionylation. Furthermore, catalase blocks the inhibition of STAT3 phosphorylation by CORMs treatment. The inhibition of glutathione synthesis by BSO was also found to attenuate STAT3 glutathionylation and its inhibition of STAT3 phosphorylation. We further found that HO-1 increases STAT3 glutathionylation and that HO-1 siRNA attenuates CORM-induced STAT3 glutathionylation. Hence...

Control of the gladiolus thrips on corms during storage

Weigel, C. A ( Charles Adolph ); Nelson, R. H; United States -- Bureau of Entomology and Plant Quarantine
Fonte: United States Department of Agriculture, Bureau of Entomology and Plant Quarantine; United States Department of Agriculture, Bureau of Entomology and Plant Quarantine ( [Washington, D.C ) Publicador: United States Department of Agriculture, Bureau of Entomology and Plant Quarantine; United States Department of Agriculture, Bureau of Entomology and Plant Quarantine ( [Washington, D.C )
Tipo: Artigo de Revista Científica Formato: 4 p. : ; 26 cm.
Publicado em //1939 ENGLISH
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Caption title.; "E-490 ; October 1939."; "Division of Truck Crop and Garden Insect Investigations."; (Statement of Responsibility) by C.A. Weigel and R.H. Nelson.

List of true bulbs, corms, and tubers (exempted under Regulation 6, Section A (1), Quarantine No. 48)

Strong, Lee A; United States -- Bureau of Entomology and Plant Quarantine
Fonte: United States Department of Agriculture, Bureau of Entomology and Plant Quarantine; United States Department of Agriculture, Bureau of Entomology and Plant Quarantine ( Washington, D.C ) Publicador: United States Department of Agriculture, Bureau of Entomology and Plant Quarantine; United States Department of Agriculture, Bureau of Entomology and Plant Quarantine ( Washington, D.C )
Tipo: Artigo de Revista Científica Formato: 3 p. : ; 27 cm.
Publicado em //1936 ENGLISH
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Caption title.; "June 1, 1936."; Signed Lee A. Strong, Chief, Bureau of Entomology and Plant Quarantine.

Sec. 301.48-6a; List of true bulbs, corms, and tubers exempted from Japanese beetle certification

Strong, Lee A; United States -- Bureau of Entomology and Plant Quarantine
Fonte: United States Department of Agriculture, Bureau of Entomology and Plant Quarantine; United States Department of Agriculture, Bureau of Entomology and Plant Quarantine ( Washington, D.C ) Publicador: United States Department of Agriculture, Bureau of Entomology and Plant Quarantine; United States Department of Agriculture, Bureau of Entomology and Plant Quarantine ( Washington, D.C )
Tipo: Artigo de Revista Científica Formato: 2 p. : ; 27 cm.
Publicado em //1940 ENGLISH
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Caption title.; "May 5, 1940."; "Lee A. Strong, Chief, Bureau of Entomology and Plant Quarantine"--P. 2.

Aestivation organ structure in Drosera subgen. Ergaleium (Droseraceae): Corms or tubers; roots or shoots?

Conran, J.
Fonte: C S I R O Publishing Publicador: C S I R O Publishing
Tipo: Artigo de Revista Científica
Publicado em //2008 EN
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27.29%
The nature of the subterranean aestivation organ in Drosera subgen. Ergaleium is reinvestigated and expanded across a wider range of taxa. The structure is confirmed to be anatomically and developmentally a stem tuber, and the adventitious growths arising along the subterranean stem are confirmed as roots. The enveloping, multi-layered, tunicate, corm-like sheath seen in some species is of epidermal and not of leaf origin, so that the structure is a tuberous condensed rhizome and not a corm. Nevertheless, the resulting structures appear to function ecologically as corms, the sheaths possibly protecting the dormant tubers from dehydration, and/or the abundant droserone pigment in the sheath tissues acting to deter fungi and other organisms. Similarly, the adventitious root-like structures on the stems are found to be anatomically roots, albeit lacking a developed root cap, and to possess well developed, epidermally derived root hairs, contrary to the suggestion of some studies that the 'hairs' were ectomycorrhizal hyphae. However, many Ergaleium species have no sheaths, or use remnant stem and/or root tissues, and some other species appear to show sheath-related hyphal associations, and highly reduced roots with few or no obvious root hairs. Thus...

Enhanced plantlet regeneration from cultured meristems in sprouting buds of saffron corms

Majourhat, Khalid; Martínez-Gómez, Pedro; Piqueras, Abel; Fernández, J. A.
Fonte: International Society for Horticultural Science Publicador: International Society for Horticultural Science
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
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4 pages.-- In: ISHS Acta Horticulturae 739: II International Symposium on Saffron Biology and Technology.; When meristems dissected from dormant corms were used for micropropagation of saffron, low and heterogeneous morphogenic potential as well as high contamination levels have been found (Piqueras and Hernandez, 2004). With the aim to prevent the above mentioned inconvenient, shoot cultures have been initiated from meristems in sprouted saffron corms before flowering. These explants showed a significant lower contamination rate (less than 30%) when cultured in a culture medium composed of QL salts, MS vitamins and 30 g/L sucrose solidified with 7.5 g/L of agar. To study the morphogenic response induced by different cytokinin types and concentrations (BAP, 2iP and TDZ), the explants were cultivated in medium supplemented with them for six weeks. After this period, the number of new shoots per initial explant, their length and quality were recorded and used as parameters for the selection of the most effective cytokinin level for multiplication. By using this new explant for the initiation of axillary shoot cultures in saffron a new micropropagation procedure has been developed suitable for the clonal propagation or ex situ germplasm conservation of selected genotypes.; Peer reviewed

Propagação do gladíolo (Gladiolus grandiflorus) andr. cv. Snow Princess: produção de material de propagação e flores pelos bulbos tipo jumbo e tipo 1; Propagation of gladiolo (Gladiolus grandiflorus) andr. cv. Snow Princess: production of corms type jumbo and type number 1

Mattos, J.R.; Simão, S.; Braga Jr., R.L.C.; Campos, H.; Moreira, C.S.
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Formato: application/pdf
Publicado em 01/01/1984 POR
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Bulbos de 2° ciclo, tipo jumbo, com 84 g/unidade e tipo 1, com 35 g/unidade, foram comparados. Verificou-se que: o tipo jumbo teve melhor rendimento de: peso de bulbos; número de bulbos; comprimento da haste floral; comprimento da espiga floral e hastes florais de melhor qualidade. Otipo 1 apresentou melhor rendimento para peso de bulbos plantados por peso de bulbos e cormilhos colhidos.; Corms of 2nd cycle, type jumbo with 84 g/unity and type 1 with 35 g/unity were compared. It was verified that: type jumbo had better profit in the production of: weight of corms, number of corms, lenght of stipe, lenght of spike and stipe of better quality. The type number 1 showed better profit for weight of corms planted by weight of corms and cormels harvest.

Propagação do gladíolo (Gladioius grandiflorus) andr. cv. Snow Princess: comportamento de seis tipos de materiais vegetativos, na produção de flores e material de propagação; Behaviour of 6 types of vegetative material for production of flowers and propagation material

Mattos, J.R.; Simão, S.; Braga Jr., R.L.C.; Campos, H.; Moreira, C.S.
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Formato: application/pdf
Publicado em 01/01/1984 POR
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Bulbos de mesmo ciclo, com pesos e tamanhos próximos, apresentaram comportamentos semelhantes para a produção de flores, bulbos e cormilhos. Os bulbos maiores, tipos 1 e 2, tiveram melhor rendimento de flores, bulbos e cormilhos que os demais tipos, decrescendo esse rendimento com a redução do tamanho dos bulbos por unidade plantada. Em função do peso plantado, as unidades menores apresentaram melhor desempenho.; Corms of the same cycle, with near weight and size, presented resembling behaviour for production of flowers, corms and cormels. Greater corms, type 1and 2showed better profit of flowers, corms and cormels than others types, decreasing this profit with the reduction of the size of corms per unity planted. In function of planted weight, the smallest units showed better profit.

Efeitos da vernalização e de fitoreguladores no desenvolvimento de Gladiolus grandiflorus; Effects of vernalization and plant regulators on growth and flowering of Gladiolus grandiflorus

Castro, Paulo R.C.; Minami, Keigo; Gil, Carolina M.; Demétrio, Clarice G.B.
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; Formato: application/pdf
Publicado em 01/01/1979 POR
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Bulbos de gladíolo foram mantidos a 22°C ou vernalizados a 4º ou 8°C por duas semanas, sendo então imersos por 24 horas em soluções de ácido giberélico (GA) 1000 ppm ou ácido indolilacético (IAA) 1000 ppm, antes de serem plantados em condições de campo. A época de emergência das brotações não foi afetada pelos tratamentos utilizados. A vernalização dos bulbos de gladíolo a 4°C, por duas semanas, promoveu aumento na altura das plantas em relação ao controle mantido a 22°C. Bulbos imersos por 24 horas em solução de GA 1000 ppm originaram plantas com menor número de folhas, sendo que bulbos vernalizados a 4°C, durante duas semanas, originaram plantas com maior número de folhas. A vernalização dos bulbos a 4º ou 8°C, por duas semanas, melhorou a qualidade das plantas de gladíolo. Não ocorreram diferenças nas datas da abertura das flores entre os tratamentos. A qualidade floral foi melhorada pela vernalização dos bulbos de gladíolo a 4º ou 8°C, durante duas semanas.; Gladiolus corms were held at 22°C or vernalized at 4° or 8°C for two weeks and then soaked for 24 hours in 1,000 ppm gibberellic acid (GA) or 1,000 ppm indolylacetic acid (IAA) before being planted in the field. There were no differences in the period of time for shoot emergence among the treatments. Treatment of gladiolus corms with 4°C for two weeks increased plant height in relation to storage at 22°C (control). Gladiolus corms soaked for 24 hours in 1...

Changes in free and conjugated indole-3-acetic acid during early stage of flower bud differentiation in Polianthes tuberosa

Ding, Shih-Fen; Chen, Wen-Shaw; Su, Stephen; Du, Huiling; Twitchin, Bruce; Bhaskar, K
Fonte: Gauthier-Villars Publicador: Gauthier-Villars
Tipo: Artigo de Revista Científica
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Tuberose (Polianthes tuberosa L. cv. Double) corms at the vegetative, early floral initiation, and flower bud differentiation stages were assayed for free indole-3-acetic acid (IAA), esterified IAA, and peptidyl IAA. The corms in the vegetative stage contained higher free IAA than those from the early floral initiation stage. Free IAA in corm tissues increased 2.7-fold at flower bud differentiation as compared to the vegetative stage. In the vegetative corms, a marked promotion of leaf differentiation was recorded. In contrast, corms from the early floral initiation stage contained less free IAA, whereas esterified IAA and peptidyl IAA increased dramatically. It is concluded that the level of free IAA in vegetative corms is correlated with leaf differentiation, and that the early floral initiation stage is correlated with a reduction in free IAA and an increase in IAA conjugates in the corms. Moreover, increases in free IAA and decreases in IAA conjugates in the floral differentiation stage, as compared to the early floral initiation stage, indicates that free IAA is correlated with flower development.

Complexos Organometálicos: caracterização preliminar de compostos de vanádio e de moléculas libertadoras de CO como potenciais fármacos

Oliveira, Ana Rita Martins Lourenço de
Fonte: Universidade Nova de Lisboa Publicador: Universidade Nova de Lisboa
Tipo: Dissertação de Mestrado
Publicado em /09/2015 POR
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Vários complexos metálicos têm sido explorados visando uma melhoria de atuais tratamentos das mais variadas doenças. Os compostos de vanádio apresentam uma capacidade de mimetizar a ação da insulina, insurgindo-se assim como uma aposta no tratamento da doença Diabetes mellitus. O monóxido de carbono (CO), outrora visto como apenas um agente tóxico, atualmente é explorado pelas suas capacidades anti-inflamatórias, tendo-se desenvolvido moléculas libertadoras de CO, os CORMs, de modo a tirar partido dos seus efeitos biológicos. Com vista a determinar a formação de aductos entre os complexos organometálicos de vanádio e diferentes proteínas e caracterizar o tipo de interação estabelecida recorreu-se à Cristalografia de raios-X. A estrutura cristalográfica do aducto VOIV(picolinato)2–tripsina bovina foi obtida a 1,09 Å de resolução, mostrando o complexo VOIV(pic)2 no centro ativo da enzima, coordenado à cadeia lateral da serina catalítica (Ser195), numa geometria octaédrica. Ensaios cinéticos preliminares realizados com o complexo VO(pic)2 foram efetuados para determinar potenciais efeitos inibitórios na atividade das enzimas lisozima da clara do ovo (HEWL) e tripsina. O potencial terapêutico dos CORMs associado à Nanotecnologia foi explorado para o [Ru(CO)3Cl(glicinato)] (CORM-3)...