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Taxonomia e filogenia das serpentes do gênero Typhlops Oppel, 1811 (Squamata: Typhlopidae) ocorrentes na Mata Atlântica brasileira

Brito, Polyanne Souto de
Fonte: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Ciências Biológicas; Biodiversidade; Biologia Estrutural e Funcional. Publicador: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Ciências Biológicas; Biodiversidade; Biologia Estrutural e Funcional.
Tipo: Dissertação Formato: application/pdf
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Dentre os táxons de serpentes distribuídos ao longo da Mata Atlântica, os Scolecophidia permanecem como um dos mais caóticos quanto à taxonomia. Das três famílias de Scolecophidia, Typhlopidae é a mais diversa com 265 espécies confinadas nas partes mais quentes do mundo e com mais de 70% pertencendo ao gênero Typhlops. O maior problema taxonômico e filogenético das espécies de Typhlops brasileiras, parece estar entre aquelas ocorrentes na Mata Atlântica: Typhlops brongersmianus e Typhlops paucisquamus, tendo em vista o baixo número de exemplares utilizados para a descrição desta última e a grande similaridade quanto à folidose entre essas espécies em simpatria. Assim sendo, este estudo teve como objetivo revisar taxonomicamente e propor a filogenia para as espécies de serpentes da família Typhlopidae ocorrentes na Mata Atlântica brasileira, tratadas pela literatura como T. brongersmianus e T. paucisquamus, utilizando caracteres merísticos e morfométricos, com o auxílio de análise estatística univariada e multivariada, além de buscas heurísticas por árvores filogenéticas mais parcimoniosas. Com base nas análises dos dados merísticos e morfométricos...

Prevalência da infecção por papilomavírus humano, herpes simplex tipo 1 e 2 e Chlamydia trachomatis em um segmento da população feminina da grande Natal/RN

Pereira, Valeska Santana de Sena
Fonte: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Ciências Biológicas; Biodiversidade; Biologia Estrutural e Funcional. Publicador: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Ciências Biológicas; Biodiversidade; Biologia Estrutural e Funcional.
Tipo: Dissertação Formato: application/pdf
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Sexually transmitted diseases (STDs) are among the largest public health problems, especially in developing countries. The acquisition of these infections during early sexual activity is common and many infections have a benign course. However, in some pathogens remain in the state of latency can be reactivated and cause productive infection that may progress to severe forms. In addition, some of them are transmitted vertically resulting in congenital infection, causing immediate damage or long-term child. The classic risk factors for sexually transmitted agents are: early onset of sexual and reproductive health, multiple sexual partners throughout life, use of oral contraceptives and co-infections with different pathogens. We present the results of a cross-sectional study aimed to estimate the prevalence of genital infection by human papillomavirus (HPV), Herpes simplex virus (HSV) and Chlamydia trachomatis (CT) in a segment of the female population of the metropolitan area Christmas, among those who enrolled voluntarily sought, Basic Health Units for the examination of cancer screening cervix in the period 2008 to 2010. All participants, a total of 261 women answered a standard questionnaire by which identified the socio-demographic characteristics...

Diversidade de invertebrados em cavernas calcárias do oeste potiguar : subsídios para a determinação de áreas prioritárias para conservação.; Invertebrate diversity in limestone caves in the Oeste Potiguar (Brazil, RN): subsidies for the determination of priority areas for conservation

Bento, Diego de Medeiros
Fonte: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Ciências Biológicas; Biodiversidade; Biologia Estrutural e Funcional. Publicador: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Ciências Biológicas; Biodiversidade; Biologia Estrutural e Funcional.
Tipo: Dissertação Formato: application/pdf
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It is estimated that the Brazilian karst areas sum about 200.000 km2. The caves, one of the main components of karst, are important windows into the biological studies on hypogean environments. In Rio Grande do Norte are known 563 caves, and 476 of them are in the municipalities of Baraúna, Felipe Guerra, Governador Dix-Sept Rosado, Apodi and Mossoró, the Western region of the State. However, like in the rest of the country, the cave fauna of the State is still poorly understood. This study used data from invertebrates harvested in 47 caves and aimed to analyze the effect of environmental change between the dry and rainy seasons in the communities of cave invertebrates, characterize these communities and evaluate the relationships between morphological and biotic variables of the caves and surroundings, and to define priority areas for conservation of cave environments of the study area from biotic parameters. Strong effects were found in the community structure of cave invertebrates due environmental changes between seasons, with values of total richness, abundance, diversity and ecological complexity significantly higher in the rainy season. It was possible to assess how the morphology of the cave and the external environment variables affect the biotic system...

Distribuição diária do comportamento da mosca da fruta Anastrepha zenildae Zucchi, (Diptera: Tephritidae) em laboratório.

Almeida, Lúcia Maria de
Fonte: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Psicobiologia; Estudos de Comportamento; Psicologia Fisiológica Publicador: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Psicobiologia; Estudos de Comportamento; Psicologia Fisiológica
Tipo: Tese de Doutorado Formato: application/pdf
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The knowledge of Anastrepha zenildae behavioral aspects combined with the biology of Tephritidae may contribute to monitoring and control programs of this fruit fly that is considered as economically important to the Rio Grande do Norte state. In order to characterize the daily activity profile of this species, we studied the behaviors of resting, locomotion, feeding, cleaning, courtship, copulation and oviposition of animals submitted to an artificial 12:12h light-dark cycle (750:1lux) with controlled temperature (26±2 °C). The observations were made with groups of 16 males and 16 females during 3 consecutive days each generation from parental to F5. Resting, locomotion, feeding and cleaning data were recorded as frequency and time of occurrence by scanning technique in 15 minutes windows per hour, with a record each minute. Courtship, copulation and oviposition were recorded as frequency, time of occurrence and duration by al occurrences technique. Resting was the most frequent behavior with males resting more than females. Locomotion was more evident in the first half of the ligh phase with higher values in females. Cleaning and feeding behaviors were more frequent in the second half of the light phase for both sexes with females eating more frequently than males. During the courtship...

Mapeamento Ambiental Como Proposta Para a Construção dos Conceitos de Biodiversidade e Cerrado no Ensino Ensino de Biologia; Envirommental Maping as proposal for construction of concepts of Biodiversity and Cerrado on Biology Teaching

CASTRO, Shaleny Costa Pereira
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Educação em Ciências e Matemática; Ciências Exatas e da Terra Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Educação em Ciências e Matemática; Ciências Exatas e da Terra
Tipo: Dissertação Formato: application/pdf
POR
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This study aimed to investigate the effectiveness of environmental mapping tool for building biological concepts Biodiversity and Cerrado students in the 2nd year of high school, in the discipline of Biology. The methodology used in this study was the participant research, where he sought to break with the dyad between subject and object and theory and practice, common features in traditional searches, so there was the construction of a more solid knowledge from a more fruitful relationship between subject-subject. Thus, it was possible to promote the critical linking between scientific knowledge and the understanding of common sense by bringing the reality of daily life of the participants themselves. Three questionnaires were applied, being the first to evaluate the proficiency level of students on the Biodiversity and Cerrado concepts and the other two questionnaires were after discussion on those concepts. The questionnaire II was applied to half of the students who participated in the research after the theoretical class, in order to verify students learning after this exposition, without completing the environmental mapping. The third questionnaire was applied to the other half of the class after the completion of the environmental mapping...

Identificação e descrição morfoanatômica e farmacognóstica das folhas de Solamum Scuticum M. Nee e bioatividade de extrato bruto em microorganismos e da fração alcaloídica em células cultivadas da linhagem vero; Identify and describe morphology, anatomy and Pharmacognostic sheets Solamum Scuticum M. Nee and bioactivity of microorganisms in crude extract and alkaloidal fraction in vero cells cultured strain

MORAES, Leslivan Ubiratan de
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Biologia; Ciências Biolóicas Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Biologia; Ciências Biolóicas
Tipo: Dissertação Formato: application/pdf
POR
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The jurubeba was identified that such species was Solanum scuticum M. Nee, from which pharmacognostic and morphanatomic data were not available to Goiás. Because of that, we tried some complementary data. For the morphoanatomic evaluation of the leaves some cuts were carried out as described by Kraus and Arduin (1997). For the phytochemical trial, it was used some methodologies as described by Costa (2001). With the phytochemical screening, were identified alkaloids, flavonoids, coumarins, anthraquinones and saponins in prospecting phytochemical. Both in phytochemical trial, as in the phytochemical prospection, it was found alkaloids. Due to the biological activities of these secondary metabolites, we obtained the alkaloid fraction, using the dust of the leaves, and we got the ethanol extract. The fractions were divided by polarity and they were evaluated by thin-layer chromatography. Microbiological evaluation was carried out to verify possible contaminants. Such evaluation did not reveal the presence of microorganisms, and it was raised the possibility of antimicrobiotic activity of the raw extract in twenty three strains of bacteria and in two yeasts. According to the antimicrobial tests, the extract presented some features, as the difficult of solubility in aqueous medium...

Ação antimicrobiana de enzimas hidrolíticas produzidas por Trichoderma asperellum e imobilizadas em blendas de polímeros biodegradáveis.; Antimicrobial action of hydrolytic enzymes produced for Trichoderma asperellum and immobilized on biodegradable polymer blends.

SILVA, Barbara Dumas Santos
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Biologia; Ciências Biolóicas Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Biologia; Ciências Biolóicas
Tipo: Dissertação Formato: application/pdf
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The hydrolytic activity of enzymes produced by Trichoderma asperellum, immobilized biodegradable films, as growth inhibitor of microorganisms was tested. The inhibitory activity was demonstrated on Aspergillus niger, Penicillium sp. and Sclerotinia sclerotiorum, microorganisms usually related to the attack and/or food contamination at the field or packaged. We used two polymer blends with different compositions, cassava starch and poly-butylene adipate-co-terephthalate (Ecoflex®, BASF Chemical Company) and other composed for polyvinyl alcohol (PVA) and polysaccharide cashew gum (PEJU). T. asperellum was induced to produce enzymes involved in the attack mycoparasite (N-acetylglucosaminidases, β-1,3-glucanases, chitinases and proteases) by the addition of crude chitin in the growth medium. The enzymes produced in major quantity were N-acetylglucosaminidase and chitinase. The pool of enzymes produced in the experiments was then used for immobilization tests. The immobilization process was performed in films by two methods: covalent and ionic bonding. In both methods, the presence of immobilized hydrolytic enzymes resulted in reduced growth of microorganisms, but the covalent immobilization of the results were more expressive. S.sclerotiorum was the microorganism most sensitive...

Sequência completa e caracterização do plasmídeo crípico pVCM1 isolado de Salmonella enterica; Complete sequence and carachterization of cryptic plasmid isoladed from Salmonella enterica

PENIDO, Ana Flávia Batista
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Biologia; Ciências Biolóicas Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Biologia; Ciências Biolóicas
Tipo: Dissertação Formato: application/pdf
POR
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Samonella spp are Gram negatives bactérias belonging to Enterobacteriaceae family. S. enterica comprise about 2.500 sorovars. These sorovars can infect a broad range, including poultry, cattle, swins and humans, and are agent causative of salmonellosis an important public health issue worldwide. Small multicopy plasmids are frequently isolated from Gram negatives and Gram positives bacterias. In Salmonella, low molecular weight plasmids are found on 10% of Salmonella strains and their biological functions are unknown. However, many plasmids in Salmonella control important properties, such as, virulence factors, heavy metals and antibiotics resistance, and utilizations of alternative carbon sources. The pVCM1 plasmid was extracted from one strain of Samonella enteretidis isolated from broilers carcass. The strains were grown in liquid or solid Luria-Bertani broth at 37 °C. The plasmid was purified, separated on 1% agarose electrophoresis and visualized by ethidium bromide staining for analysis. Plasmid was digested with EcoRI enzyme and subcloned in the pUC18 vector.The plasmidal stability was evaluated, inoculating E. coli cells transformated with pVCM1 plasmid (cloned in pUC18) in liquid Luria-bertani broth supplemented with ampicillin. The pVCM1 was stable after 240 generations. The total DNA sequence of plasmid pVCM1 has 1981 pb. Genbank search resulted that pVCM1 showed 99% of identity with pB and 92% with pJ...

Influência do estresse hídrico, riqueza e abundância de espécies nativas sobre o potencial invasor de uma Poaceae exótica; Influence of desiccation, species richness and species abundance on invasiveness of a exotic Poaceae

MICHELAN, Thaísa Sala
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia
Tipo: Dissertação Formato: application/pdf
POR
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Exotic species represent strong threats to biodiversity. This concern is especially valid in freshwater ecosystems due to their high biodiversity and the highest rates of extinction, compared with terrestrial and marine ecosystems. The increasing habitat complexity provided by the presence of macrophytes may partially explain the high diversity of aquatic ecosystems. However, depending on the density and other particular characteristics of these plant, the diversity may be negatively affected. In this context, Urochloa subquadripara, a species native to Africa, is an aquatic macrophyte weed occurring in various natural and artificial aquatic ecosystems in America. In a small scale, the occurrence of this exotic species increases with reducing natives species richness; however, in a large scale U. subquadripara invades sites with more richness of native species. This controversy is typical of observational studies in invasion biology and it demand experiments to test effects of abiotic and biotic resistance on the invasive potential of this exotic. Accordingly, two experiments were conducted in order to test the following hypotheses: (i) the regeneration and colonization of U. subquadripara decreases with increasing time of exposure to dry...

Estrutura hierárquica na resposta das distribuições geográficas de plantas do Cerrado à mudanças climáticas; Hierarchical structure in the response of geographic distributions of Cerrado plants to climate change

SOUSA, Nayara Pereira Rezende de
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia
Tipo: Dissertação Formato: application/pdf
POR
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The climate can be considered an extremely important factor in vegetation distribution and its characteristics in a global context. Climate changes affect the global distribution of vegetation in the distant past and probably will affect in the future. Spatial distribution models of species (SDMs), also known as niche models are one of the best current tools for predicting climate-induced changes on the distribution of species. In general, studies of the consequences of climate changes on the distribution of biomes include only the geographic distribution of the biome or the species are analyzed individually. The availability of climatic and ecological data on a large scale, in addition to more efficient procedures for obtaining data from geographic information systems, optimization programs and greater computing power favor the process of modeling the potential distribution, and broaden the effectiveness of results available by the SDMS. The main objective of this study is to evaluate whether there is a shift in the hierarchical structure of future distributions of biome, phytoeeological regions and plants species of the biome under the effect of climate changes. Consequently, we evaluate some effects of climate changes on the pattern of species richness and pattern of future distributions of the biome...

Modelagem de nicho em espécies de plantas do Cerrado e distribuição espacial da variabilidade genética; Niche modeling of plants species from the Cerrado and spatial distribution of genetic variability

CAVALCANTI, Fábio André Gomes S
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia
Tipo: Dissertação Formato: application/pdf
POR
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Ecological and evolutionary factors may explain the patterns of intrapopulation variability across the geographic distribution of a species. More suitable habitats, in ecological terms, allow the survival of greater number of individuals, which increases the genetic variability in the population, while in less suitable habitats, because of smaller population size, individuals have lower reproductive rate and higher mortality rate, these populations may lead to loss of genetic variability and extinction. In this context, the goal of this study was to use meta-analysis techniques to relate the genetic variables (observed and expected heterozygosity and inbreeding) with the models of potential geographical distribution of nine species of Cerrado plants. In addition, genetic variables were correlated with human occupation at sites where plant species occurred. For this work were used 11 studies, in which genetic data were obtained from literature and the points of occurrence in databases and also the literature. Five species distribution models (Maxent, Mahalanobis, Euclidian, Domain and Bioclim) were used and then superimposed on a combined basis (i.e Ensemble forecasting). Were performed Pearson correlations between genetic variables and environmental and human occupation. The coefficients of these correlations were matched using a meta-analysis to find a general correlation studies. The correlations between genetic variables with the ecological variable (i.e environmental suitability) and human occupation tended to zero. Although significant values have been obtained with studies of isozymes for the human index (r = 0.57 P = 0.01) related...

Suficiência taxonômica, resolução numérica e grupos substitutos: uma análise para a comunidade fitoplanctônica de ambiente lótico; Taxonomic sufficiency, numerical and groups replacements: an analysis for the phytoplankton community of lotic

CARNEIRO, Fernanda Melo
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia
Tipo: Dissertação Formato: application/pdf
POR
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Classification systems are necessary to organize the huge complexity of biological systems. Frequently, these systems are useful to studies focusing on environmental monitoring, conservation plans and biodiversity assessments. Studies on phytoplankton ecology are, in general, conducted with the identification of organisms up to the species level. This is a costly, laborious and complex task that demands experienced biologists. However, for some purposes, higher taxonomic levels may be enough, mainly when main trends are identified by either data at low or higher resolution (taxonomic and numeric). In this study, it was evaluated if the temporal trajectories described by a lotic phytoplankton community, which were first summarized by an ordination technique, were dependent or not on the taxonomic/numerical resolution used to represent the data. The phytoplankton classification system in functional groups was also contrasted against simple taxonomic classifications in order to verify if they really offer distinct patterns of ordination. Procrustean analyses indicated that patterns of ordination generated by incidence data of genus were significantly concordant with the patterns generated by density of species. Temporal trajectories of scores derived from functional groups significantly matched those derived from analyses based on the quantitative data (density or biovolume) of genus or family. Thus...

Diversidade genética de hilídeos do Brasil Central; Genetic diversity hylids Central Brazil

Oliveira, Hugo Henrique Pádua de
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Genética e Biologia Molecular; Instituto de Ciências Biológicas - ICB (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Genética e Biologia Molecular; Instituto de Ciências Biológicas - ICB (RG)
Tipo: Dissertação Formato: application/pdf
POR
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The anurans are a group of vertebrates with wide geographical distribution that obtained highly evolutionary success, being part of a lineage descendant of early tetrapods, the first vertebrates to conquer the terrestrial environment. Anurans are divided into two suborders: Archaeobatrachia and Neobatrachia. Within the suborder Neobatrachia there is Bufonoidea superfamily, which includes the three families of higher abundance in the neotropical region: Leptodactylidae, Bufonidae and Hylidae. The aim of this study was to analyze cytogenetically species of the family Hylidae of areas of the Cerrado of Goiás, by the technique of conventional cytogenetics and by silver nitrate staining. The metaphases were obtained directly from cell suspensions of spleen, liver and bone marrow. The slides were stained with Giemsa at 8%, to determine the diploid number and chromosome morphology. The species Hypsiboas multifasciatu and Hypsiboas raniceps presented the diploid number 2n=24 and fundamental number FN=48 with positive marks for NORs in chromosomes 8 and 10 respectively. The species Hypsiboas lundii presented diploid number 2n=24 and fundamental number FN=46 due to the presence of acrocentric chromosome in pair 6. The RONs for this species were identified in pericentromeric regions of short arms of chromosome 5 and in telomeric regions of short arms of chromosome 8. Within the genus Hypsiboas...

Sistema distílico e biologia reprodutiva de cinco espécies de Psychotria L. (Rubiaceae) em dois fragmentos do sudeste Goiano; Distylous system and reproductive biology of five species of Psychotria L. (Rubiaceae) in two fragments southeast Goiás

Sá, Túlio Freitas Filgueira de
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biodiversidade Vegetal (ICB); Instituto de Ciências Biológicas - ICB (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biodiversidade Vegetal (ICB); Instituto de Ciências Biológicas - ICB (RG)
Tipo: Dissertação Formato: application/pdf
POR
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Distyly is a reproductive strategy to promote cross-pollination and difficult self-pollination, characterized by the presence of two floral morphs, the pin and the thrum morph. The study aimed to describe the phenological behavior and investigate aspects related to the distylous system of five Psychotria species, such as the pollination biology, the floral morphology, the isoplety and the incompatibility system of the species. The studies were performed in the "Parque Municipal da Mata do Setor Santa Cruz" and "in the Pasto do Pedrinho", in the municipality of Catalão, Goiás. The five species of the genus Psychotria were: P. hoffmannseggiana, P. capitata, P. prunifolia, P. deflexa. and P. trichophoroides. The flowers were collected and fixed in 70% ethanol for the morphometric analysis. The phenology was held for one year for each species. The incompatibility system was tested using hand pollination. The flower visitors were collected, labeled and identified. The results indicate that P. hoffmannseggiana, P. capitata, P. deflexa and P. trichophoroides present distyly, only P. prunifolia was considered as pin-monomorphic . In the reproductive system, P. prunifolia was considered as self-compatible. Although, P. hoffmannseggiana...

Prospecção de enzimas de solo de Cerrado sob plantio de cana-de-açúcar; Study of enzymes from cerrado soil under sugarcane cultivation

Purcena, Luiza Luanna Amorim
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG)
Tipo: Tese de Doutorado Formato: application/pdf
POR
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The increasing land area cultivated with sugarcane in Goiás reflects the economic importance of this monoculture not only to Goiás, but also to Brazil. However, the native areas of Cerrado converted to monoculture planting must be monitored since the effects in the biochemical, biological and physicochemical characteristics of soil have already been reported. In this study, the effect of organic (OM) and conventional management (CM) of sugarcane on hidrolases and oxidative enzymes and the results were compared to those for native soil of Cerrado (NS). It was quantified the activity of acid and alkaline phosphomonesterases, β-glucosidases, peroxidases and phenol oxidases. It was also determined the physicochemical parameters such as granulometry (percentage of clay, silt and sand) and content of soil organic matter (SOM), pH, Inorganic phosphorus (P + ), calcium (Ca +2 ), Potassium (K + ). Magnesium (Mg +2 ), Aluminum (Al +3 ), potential acidity total (H+Al) saturation in Aluminum (M%), saturation of alkalis (V%), cation exchangeable capacity (CEC) and the relationship between Ca/CEC, K/CEC and Mg/CEC content as well as the relationships of the cations Ca/K, Ca/Mg...

Investigação do potencial mutagênico e recombinogênico dos combinados gemcitabina+doxorrubicina e gemcitabina+cisplatina em células somáticas de Drosophila melanogaster; Investigation of mutagenic and recombinogenic combination of gemcitabine + cisplatin and gemcitabine + doxorubicin in somatic cells of Drosophila melanogaster

Oliveira, Igor Gomes de
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG)
Tipo: Dissertação Formato: application/pdf
POR
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Clinical studies have shown that the combinations of chemotherapeutic drugs gemcitabine (GEM) plus cisplatin (CIS) and gemcitabine (GEM) plus doxorubicin (DXR) exert important cytotoxic activity against several types of cancer in advanced stages as well as metastatic cancer. CIS, DXR and GEM have different mechanisms of action. GEM is a pro-drug that must be phosphorylated by deoxycytidine kinase to evolve into its active form. Both gemcitabine diphosphate and gemcitabine triphosphate inhibit processes required for DNA synthesis. CIS induces a variety of DNA structural changes, mainly intra- and interstrand cross-links between adjacent purine bases. DXR acts as a topoisomerase II inhibitor. This study compares the genetic toxicity effects induced by GEM+CIS and GEM+DXR co-treatments with the single drug treatments. We used the Somatic Mutation And Recombination Test (SMART), which simultaneously detects and quantifies mutagenic and recombinogenic toxicological endpoints through loss of heterozygosity of two genetic markers involved in the metabolic pathways of the Drosophila melanogaster wing hairs formation. Using the standard cross, the third-stage larvae were chronically treated with different concentrations of GEM (0.008, 0.010...

Caracterização de anidrases carbônicas do fungo Paracoccidioides brasiliensis; Characterization of carbonic anhydrases of Paracoccidioides brasiliensis

Tomazett, Mariana Vieira
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG)
Tipo: Dissertação Formato: application/pdf
POR
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Paracoccidioidomycosis (PCM) is the most important endemic deep mycosis in Latin America. Understanding of the complex interactions between the fungus and host must include the identification of gene expression patterns during infection. Carbonic anhydrase (CA) belongs to the family of zinc metalloenzymes that catalyzes the reversible hydratation of carbon dioxide to bicarbonate. Transcriptional studies have shown that carbonic anhydrase of P. brasiliensis is expressed in yeast cells recovered from liver of infected mice. In the present work, we characterized the cDNAs encoding for four carbonic anhydrases of P.brasiliensis (PbCA1, PbCA2, PbCA3, PbCA4). Recombinant PbCA1, 3 and 4 were obtained in heterologous systems with 33 kDa, 28 kDa and 32 kDa respectively. Mass spectrometry analysis confirmed the sequences of the produced proteins. The expression of PbCAs transcripts was evaluated by using real-time RT-PCR in mycelium, yeast cells and mycelium to yeast transition, in yeast cells exposed to CO2 and yeast cells recovery directly from liver and spleen. In the presence of CO2, PbCA1, PbCA2 and PbCA4 gene expression was reduced in the course of time. PbCA1 transcript expression was induced during the mycelium to yeast transition. PbCA2 and PbCA4 gene expression was higher in yeast cells...

Estudo sobre o comportamento de apetência de diferentes estágios do carrapato Amblyomma cajennense (Acari: Ixoidae); Study on the behavior of palatability of different stages of the tick Amblyomma cajennense (Acari: Ixoidae)

ASSAL, Flávio Ezzeddine El
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Ecologia e Evolução; Ciências Biológicas - Biologia
Tipo: Dissertação Formato: application/pdf
POR
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This paper evaluates the behavior of appetite expressed in different stages of the ticks Amblyomma cajennense testing the hypothesis that nymphs and adults of this species have strategy of game play while the larvae ambush. To this end, through observations, we sought to describe and propose a classification of appetite expressed by these different stages comparing strategies appetite displayed by different instars of the species with the express adult Rhipicephalus sanguineus on the same environmental conditions. Experimentally, the expressions of these behaviors face of different stimuli (CO2, equine odor, shading, radiant heat, and the 'air control') were evaluated. For this, we collected ticks of the species in horses and dogs naturally infested Center for Zoonosis Control in Goiânia-GO, which were created in rabbits and multiplied in the vivarium of the Center for Veterinary Parasitology, Federal University of Goiás (CPV / UFG ). The ratings of the behaviors that occurred in a room of the Centre -CPV/UFG were held in a glass box with removable cover, having a dozen plastic rods for the expression of search strategies. This glass chamber showed temperature conditions (25 º C + / -3 ° C), relative humidity (80%

Mapa proteômico de espécies filogenéticas do complexo Paracoccidioides; Proteomic maps of members of the Paracoccidioides complex

Pigosso, Laurine Lacerda
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG)
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
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The genus Paracoccidioides comprises a complex of phylogenetic species of dimorphic pathogenic fungi, the etiologic agents of paracoccidioidomycosis (PCM), a disease confined to Latin America and of marked relevance in its endemic areas due to its high frequency and severity. The members of the Paracoccidioides genus are distributed in distinct phylogenetic species (S1, PS2, PS3 and 01-like) that potentially differ in their biochemical and molecular characteristics. In this work, we performed the proteomic characterization of different members of the genus Paracoccidioides. We compared the proteomic profiles of Pb01 (01-like), Pb2 (PS2), Pb339 (S1) and PbEPM83 (PS3) using 2D electrophoresis and mass spectrometry. The proteins/isoforms were selected based on the staining intensity of the spots as determined by image analysis. The proteins/isoforms were in-gel digested and identified by peptide mass fingerprinting and ion fragmentation. A total of 714 spots were detected, of which 343 were analyzed. From these spots, 301 represented differentially expressed proteins/isoforms among the four analyzed isolates, as determined by ANOVA. After applying the FDR correction, a total of 267 spots were determined to be differentially expressed. From the total...

Variabilidade e história evolutiva do gene HLA-E; Variability and evolutionary history of HLA-E gene

Felício, Leandro Prado
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Biologia (ICB); Instituto de Ciências Biológicas - ICB (RG)
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
85.92%
The HLA-E locus is a Human Major Histocompatibility Complex (MHC) gene associated with immune-modulation and suppression of the immune response by the interaction with specific NK and T cell receptors. The HLA-E gene is considered the most conserved locus in the human HLA; however, this low variability might be a consequence of the scarce number of studies focusing this subject. In this mastering thesis we assessed the HLA-E coding and 3’ untranslated region variability in a group of individuals from Brazil and the results were evaluated together with data from the 1000Genomes Consortium. Altogether, only 28 variation sites were found in approximately 2724 bp evaluated. These variation sites were arranged into 33 haplotypes, most of them (98.2%) encoding one of the two HLA-E molecules found worldwide, i.e., the molecules associated with the allele groups E*01:01 and E*01:03. Interestingly, 85% of all haplotypes were represented by only three different sequences, each of them associated with one of the main known HLA-E coding alleles, E*01:01:01, E*01:03:01 and E*01:03:02, all of them found worldwide. This phenomenon, together with the comparisons with other primate sequences, reveals that these two main allele groups (and molecules) arose early before human speciation...