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Free amino acids of Tronchuda Cabbage (Brassica oleracea L. Var. costata DC): influence of leaf position (internal or external) and collection time

Oliveira, Andreia P.; Pereira, D.M.; Andrade, P.B.; Valentão, P.; Sousa, C.; Pereira, J.A.; Bento, Albino; Rodrigues, M.A.; Seabra, R.M.; Silva, B.M.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
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The free amino acid profile of 18 samples of tronchuda cabbage (Brassica oleracea L. var. costata DC) leaves, harvested at three different months, was determined by HPLC/UV−vis. The tronchuda cabbage leaves total free amino acid content varied from 3.3 to 14.4 g/kg fresh weight. Generally, arginine was the major compound, followed by proline, threonine, glutamine, cysteine, and glutamic acid. This study indicates that free amino acids are not similarly distributed: in external leaves, proline and arginine were the major free amino acids, while in internal ones, arginine was the main free amino acid, followed by threonine, glutamine, and cysteine. Significant differences were observed for valine, proline, arginine, leucine, cysteine, lysine, histidine, and tyrosine contents. The levels of some free amino acids were significantly affected by the collection period. In external leaves, this occurred with glutamic acid, serine, valine, leucine, cysteine, and ornithine contents, while in internal leaves, it occurred with aspartic acid, arginine, and total contents.

Phenolic compounds in external leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC)

Ferreres, F.; Valentão, P.; Llorach, R.; Pinheiro, C.; Cardoso, L.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
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Glycosylated kaempferol derivatives from the external leaves of tronchuda cabbage ( Brassica oleracea L. var. costataDC) characterized by reversed-phase HPLC-DAD-MS/MS-ESI were kaempferol 3- Osophorotrioside- 7-O-glucoside, kaempferol 3-O- (methoxycaffeoyl/caffeoyl)sophoroside-7- O-glucoside, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-sophorotrioside-7-O-sophoroside, kaempferol 3- O-sophoroside-7- O-sophoroside, kaempferol 3- O-tetraglucoside-7- O-sophoroside, kaempferol 3- O-(sinapoyl/caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(feruloyl/caffeoyl)sophoroside-7- O-glucoside, kaempferol 3-O-sophorotrioside, kaempferol 3-O-(sinapoyl)sophoroside, kaempferol 3-O- (feruloyl)sophorotrioside, kaempferol 3- O-(feruloyl)sophoroside, kaempferol 3- O-sophoroside, and kaempferol 3- O-glucoside. These acylated derivatives are reported for the first time in nature, with the exception of kaempferol 3- O-(sinapoyl)sophoroside. Quantification of the identified compounds was achieved by HPLC-DAD and carried out in samples cultivated under conventional or organic practices and collected at different times. In general, samples from organic production exhibited higher total phenolics content than those from conventional practices collected in the same period.

Influence of two fertilization regimes on the amounts of organic acids and phenolic compounds of tronchuda cabbage (Brassica oleracea L. var. costata DC)

Sousa, C.; Valentão, P.; Rangel, Joana; Lopes, Graciliana; Pereira, J.A.; Ferreres, F.; Seabra, R.M.; Andrade, P.B.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.38%
A phytochemical study was undertaken on tronchuda cabbage ( Brassica oleracea L. var. costata DC) cultivated under conventional and organic practices and collected at different times. Six organic acids (aconitic, citric, ascorbic, malic, shikimic, and fumaric acids) were identified and quantified by HPLC-UV. Qualitative and quantitative differences were noted between internal and external leaves. Analysis of the phenolics of the internal leaves was achieved by HPLC-DAD, and the phenolic profile obtained was revealed to be distinct from that of the external leaves. By this means were identified and quantified 11 compounds: 3- p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3- O-(caffeoyl)sophoroside-7- O-glucoside, kaempferol 3- O-(sinapoyl)sophoroside-7- Oglucoside, kaempferol 3- O-(feruloyl)sophoroside-7- O-glucoside, kaempferol 3- O-sophoroside, two isomeric forms of 1,2-disinapoylgentiobiose, 1-sinapoyl-2-feruloylgentiobiose, 1,2,2¢-trisinapoylgentiobiose, and 1,2¢-disinapoyl-2-feruloylgentiobiose. In general, internal leaves exhibited more constant chemical profiles.

Antioxidative properties of tronchuda cabbage (Brassica oleracea L. var. costata DC) external leaves against DPPH, superoxide radical, hydroxyl radical and hypochlorous acid

Vrchovska, V.; Sousa, C.; Valentão, P.; Ferreres, F.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.38%
The ability of the aqueous extract of tronchuda cabbage (Brassica oleracea L. var. costata DC) external leaves to act as a scavenger of DPPH- and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid) was investigated. A phytochemical study was also undertaken, and thirteen phenolic compounds and five organic acids were identified and quantified. Tronchuda cabbage extracts exhibited antioxidant capacity in a concentration-dependent manner in all assays, although some pro-oxidant effect was also noticed. The samples with higher phenolic and organic acid contents displayed the major antioxidant potentials.

Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phytochemical characterization and antioxidant potential

Ferreres, F.; Sousa, C.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Andrade, P.B.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.38%
Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds were studied for their chemical composition and antioxidant capacity. Thirteen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-MS/MS-ESI and HPLC-DAD, respectively: two sinapoylgentiobiose isomers, three sinapoylglucose isomers, kaempferol-3-(sinapoyl)sophorotrioside-7-glucoside, sinapoylcholine, kaempferol-3,7-diglucoside-40-(sinapoyl)glucoside, three disinapoylgentiobiose isomers, 1,2,20-trisinapoylgentiobiose and 1,2-disinapoylglucose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids) were also identified and quantified by HPLC-UV. The aqueous extract of tronchuda cabbage seeds was investigated for its capacity to act as a scavenger of DPPH- radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid), exhibiting antioxidant capacity in a concentration-dependent manner against all radicals. These results may be attributed to the high content of hydroxycinnamic derivatives and ascorbic acid.

Screening of antioxidant compounds during sprouting of Brassica oleracea L. var. costata DC

Sousa, C.; Lopes, Graciliana; Pereira, D.M.; Taveira, M.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Baptista, Paula; Ferreres, F.; Andrade, P.B.
Fonte: Bentham Science Publisher Publicador: Bentham Science Publisher
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.38%
The changes in antioxidant compounds of Brassica oleracea L. var. costata DC seeds were monitored during the first twelve days of seedling development. Sprouts were screened at time intervals of two days for phenolic compounds and organic acids. The identified phenolic compounds included esters of sinapic acid with glucose, gentiobiose and kaempferol, as well as sinapoylcholine. The organic acids were oxalic, aconitic, citric, pyruvic, malic, shikimic, and fumaric acids. During germination, a depletion of phenolic compounds was observed, although no qualitative changes were seen. Among individual compounds, kaempferol, choline and glucose esters of sinapic acid showed a marked decrease between days two and six, whereas the changes in gentiobiose esters of sinapic acid were smaller. The total organic acids content increased rapidly during the first four days, with less significant variations thereafter. Malic acid, the major organic acid found in sprouts, greatly contributed to this result though oxalic, pyruvic, and fumaric acids also increased in the same manner. In contrast, aconitic, citric and shikimic acids showed decreases between days two and twelve of germination.

Multivariate analysis of of Tronchuda Cabbage (Brassica oleracea L. var. costata DC) phenolics: influence of fertilizers

Sousa, C.; Pereira, D.M.; Pereira, J.A.; Rodrigues, M.A.; Bento, Albino; Dopico-García, S.; Lopes, Graciliana; Valentão, P.; Seabra, R.M.; Andrade, P.B.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.38%
A field experiment was carried out to investigate the effect of fertilization level on the phenolic composition of tronchuda cabbage (Brassica oleracea L. var. costata DC) external and internal leaves. Eight different plots were constituted: a control without fertilization, one with organic matter, and six experiments with conventional fertilizers (nitrogen, boron, and sulfur, two levels each). The phenolic compounds were analyzed by reversed-phase HPLC-DAD. External and internal leaves revealed distinct qualitative composition. In the internal leaves were found 15 phenolics (5 kaempferol and 10 cinnamic acid derivatives), whereas the external leaves presented 3-p-coumaroylquinic acid and 13 kaempferol derivatives. Principal component analysis (PCA) was applied to assess the relationships between phenolic compounds, agronomical practices, and harvesting time. Samples obtained with conventional practices were quite effectively separated from organic samples, for both types of leaves. In general, samples developed without any fertilization presented the highest phenolics amounts: external and internal leaves contained 1.4- and 4.6-fold more phenolic compounds than the ones that received conventional fertilizer, respectively...

Screening of antioxidant phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC.

Ferreres, F.; Sousa, C.; Pereira, D.M.; Valentão, P.; Taveira, M.; Martins, Anabela; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
Fonte: Bentham Science Publicador: Bentham Science
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.43%
The phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC were screened by HPLC-DAD-MS/MS. Thirty seven compounds were characterized, which included chlorogenic acids, flavonoids (the majority of them were hydroxycinnamic acid esters of kaempferol and quercetin glycosides) and hydroxycinnamic acyl glycosides (with predominance of synapoyl gentiobiosides). The antioxidant capacity of the shoots was assessed against DPPH radical and two reactive oxygen species (superoxide radical and hypochlorous acid). A strong concentrationdependent antioxidative capacity was verified in the DPPH and superoxide radicals assays, but a reduced effect was noticed against hypochlorous acid. The results obtained indicate that the in vitro production of B. oleracea var. costata shoots can become important in the obtention of a noticeable dietary source of compounds with health protective potential.

Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala).

Ferreres, F.; Fernandes, F.; Oliveira, J.M.A.; Valentão, P.; Pereira, J.A.; Andrade, P.B.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.39%
Phenolic and organic acid profiles of aqueous extracts from Pieris brassicae material and the host kale (Brassica oleracea L. var. acephala) leaves were determined by HPLC/UV–DAD/MSn-ESI and HPLC–UV, respectively. The identified phenolics included acylated and nonacylated flavonoid glycosides, hydroxycinnamic acyl gentiobiosides, and sulphate phenolics. Kale exhibited the highest content (11 g/kg lyophilized extract), while no phenolics were identified in the butterflies or exuviae. Nine different organic acids were characterized in the materials, with kale showing the highest amount (112 g/kg lyophilized extract). With the exception of the exuviae extract, the rest were screened for bioactivity. Using spectrophotometric microassays, all exhibited antiradical capacity against DPPH and NO in a concentrationdependent way, whereas only kale and excrement extracts were active against superoxide. All displayed activity on intestinal smooth muscle, albeit with distinct relaxation–contraction profiles. Larvae and butterfly extracts were more efficacious for intestinal relaxation than was kale extract, whereas excrement extract evoked only contractions, thus evidencing their different compositions. Collectively, these results show that P. brassicae sequesters and metabolizes kale’s phenolic compounds. Moreover...

Volatile constituents throughout Brassica oleracea L. var. acephala germination

Fernandes, F.; Pinho, P. Guedes de; Valentão, P.; Pereira, J.A.; Andrade, P.B.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.38%
In this work, the volatile composition of kale (Brassica oleracea L. var. acephala) and its variation during germination were monitored during the first 9 days of seedling development by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/ion trap-mass spectrometry (GC/IT-MS). Differences were found among the materials in the distinct analyzed periods. A total of 66 volatile compounds, distributed in several chemical classes, were determined: alcohols, carbonyl compounds (ketones, aldehydes, and esters), norisoprenoids, and terpenes, among others, sulfur compounds being the most abundant group in seeds and sprouts that exhibited allyl isothiocyanate as the major compound. Leaves of fully developed ground plant had the highest content of norisoprenoids, alcohols, and carbonyl compounds; in opposition, they showed lower levels of sulfur compounds, suggesting that these are important molecules for the development of kale, whereas the others are produced mainly during its growth.

Abundância de larvas e pupas de Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae) em Brassica oleracea l.var. Acephala d.c., mortalidade causada por parasitóides e biologia de Tetrastichus sokolowskii Kurdjmov, 1912 (Hymenoptera: Eulophidae)

Ferronatto, Edna Maria de Oliveira
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
POR
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86.47%
Com o objetivo de estimar-se a densidade de Plutella xylostella (Linnaeus, 1758) numa cultura de Brassica oleracea L. var. acephala D C., cultivar "tronchuda portuguesa", foram realizadas amostras a intervalos regulares durante a estação 1980/81, na Estação Experimental Fitotécnica de Viamão, Distrito de Águas Claras, Município de Viamão, R.S. A área experimental consistiu de 500 plantas de couve; a unidade de amostra foi a folha. Na estação de 1980/81, em paralelo, estimou-se a mortalidade ocasionada por parasitóides aos estágios de larva e pupa. Acompanharam-se durante todo o período de ocorrência em campo as variações nas taxas de parasitismo em relação ao nível da população da praga. As três espécies predominantes de parasitóides foram Tetrastichus sokolowskii Kurdjmov, 1912. Apanteles piccotrichosus Blanchard, 1943 e Spilochalcis sp. aff. a fulvovariegata (Cameron, 1884). Em 1981/82, o parasitismo foi estimado independentemente da densidade do hospedeiro. Para as amostras, consideraram-se apenas os dois últimos estádios larvais. Nas condições em que foram realizados os experimentos, a ocorrência de P. xylostella e de seu complexo de parasitóides restringiu-se a um curto período do ano. Fora deste...

Efeito inibitório na germinação, induzido pelo extrato de couve (Brassica oleracea L. var. acephala DC.)

Lucchesi,A.A.; Oliveira,R.F.
Fonte: Universidade de São Paulo; Escola Superior de Agricultura Publicador: Universidade de São Paulo; Escola Superior de Agricultura
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/1988 PT
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Com a finalidade de se estudar as possíveis propriedades alelopáticas da couve (Brassica oleracea L. var. acephala DC), foi conduzido um bioensaio, em condições de laboratório, na E.S.A."Luiz de Queiroz", em Piracicaba, SP. Foram utilizadas sementes de tomate (Lycopersicon esculentum Mill. cv.Santa Cruz) colocadas para germinar em soluções com diferentes concentrações (0; 25; 50 e 100%) do extrato obtido da parte aérea (folhas) da couve. Constataram-se efeitos pronunciadamente inibitórios de germinação das sementes de tomate nas maiores concentrações do extrato de couve. As plântulas que germinaram, nas parcelas tratadas com o extrato, mostraram-se com o crescimento reduzido, morfo-fisiologicamente anormais, e com maior tempo para o início da germinação. Todas essas características foram mais pronunciadas nas parcelas com maiores concentrações do extrato.

Antioxidative properties of tronchuda cabbage (Brassica oleracea L. var. costata DC) external leaves against DPPH, superoxide radical, hydroxyl radical and hypochlorous acid

Sousa, Carla; Vrchovská, Vendula; Valentão, Patricia; Ferreres, Federico; Pereira, José Alberto; Seabra, Rosa M.; Andrade, Paula B.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
Relevância na Pesquisa
96.38%
10 pages, 6 figures, 4 tables.-- Printed version published 2006.; The ability of the aqueous extract of tronchuda cabbage (Brassica oleracea L. var. costata DC) external leaves to act as a scavenger of DPPH and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid) was investigated. A phytochemical study was also undertaken, and thirteen phenolic compounds and five organic acids were identified and quantified. Tronchuda cabbage extracts exhibited antioxidant capacity in a concentration-dependent manner in all assays, although some pro-oxidant effect was also noticed. The samples with higher phenolic and organic acid contents displayed the major antioxidant potentials.; The authors are grateful to Fundaçäo para a Ciência e Tecnologia (POCI/AGR/57399/2004) for financial support of this work. Vendula Vrchovská is grateful to the European Union Erasmus/Socrates II Programme for a grant (MSM 0021620822).; Peer reviewed

Phenolic Compounds in External Leaves of Tronchuda Cabbage (Brassica oleracea L. var. costata DC)

Ferreres, Federico; Valentão, Patricia; Llorach, Rafael; Pinheiro, Cristina; Cardoso, Lígia; Pereira, José Alberto; Sousa, Carla; Seabra, Rosa M.; Andrade, Paula B.
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
Relevância na Pesquisa
96.38%
7 pages, 3 tables, 4 figures.; Glycosylated kaempferol derivatives from the external leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC) characterized by reversed-phase HPLC-DAD-MS/MS-ESI were kaempferol 3-O-sophorotrioside-7-O-glucoside, kaempferol 3-O- (methoxycaffeoyl/caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-sophorotrioside-7-O-sophoroside, kaempferol 3-O-sophoroside-7-O-sophoroside, kaempferol 3-O-tetraglucoside-7-O-sophoroside, kaempferol 3-O-(sinapoyl/caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(feruloyl/caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-sophorotrioside, kaempferol 3-O-(sinapoyl)sophoroside, kaempferol 3-O-(feruloyl)sophorotrioside, kaempferol 3-O-(feruloyl)sophoroside, kaempferol 3-O-sophoroside, and kaempferol 3-O-glucoside. These acylated derivatives are reported for the first time in nature, with the exception of kaempferol 3-O-(sinapoyl)sophoroside. Quantification of the identified compounds was achieved by HPLC-DAD and carried out in samples cultivated under conventional or organic practices and collected at different times. In general, samples from organic production exhibited higher total phenolics content than those from conventional practices collected in the same period.; We are grateful to Fundaçäo para a Ciência e Tecnologia (POCTI/AGR/57399/2004) for financial support of this work.; Peer reviewed

Multivariate Analysis of Tronchuda Cabbage (Brassica oleracea L. var. costata DC) Phenolics: Influence of Fertilizers

Sousa, Carla; Pereira, David M.; Pereira, José Alberto; Bento, Albino; Rodrigues, M. Angelo; Dopico-García, Sonia; Valentão, Patricia; Lopes, Graciliana; Ferreres, Federico; Seabra, Rosa M.; Andrade, Paula B.
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
Relevância na Pesquisa
96.38%
9 pages, 5 figures, 2 tables.; A field experiment was carried out to investigate the effect of fertilization level on the phenolic composition of tronchuda cabbage (Brassica oleracea L. var. costata DC) external and internal leaves. Eight different plots were constituted: a control without fertilization, one with organic matter, and six experiments with conventional fertilizers (nitrogen, boron, and sulfur, two levels each). The phenolic compounds were analyzed by reversed-phase HPLC-DAD. External and internal leaves revealed distinct qualitative composition. In the internal leaves were found 15 phenolics (5 kaempferol and 10 cinnamic acid derivatives), whereas the external leaves presented 3-p-coumaroylquinic acid and 13 kaempferol derivatives. Principal component analysis (PCA) was applied to assess the relationships between phenolic compounds, agronomical practices, and harvesting time. Samples obtained with conventional practices were quite effectively separated from organic samples, for both types of leaves. In general, samples developed without any fertilization presented the highest phenolics amounts: external and internal leaves contained 1.4- and 4.6-fold more phenolic compounds than the ones that received conventional fertilizer...

Tronchuda Cabbage (Brassica oleracea L. var. costata DC): Scavenger of Reactive Nitrogen Species

Sousa, Carla; Valentão, Patricia; Ferreres, Federico; Seabra, Rosa M.; Andrade, Paula B.
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
Relevância na Pesquisa
96.38%
7 pages, 6 figures, 4 tables.; The ability of tronchuda cabbage (Brassica oleracea L. var. costata DC) to act as a scavenger of the reactive nitrogen species nitric oxide and peroxynitrite was investigated. The aqueous extracts obtained from tronchuda cabbage seeds and from its external and internal leaves exhibited a concentration dependent scavenging capacity. The antioxidant potential observed against the two reactive species was as follows: seeds > external leaves > internal leaves. In order to establish a possible correlation with the chemical composition of the extracts, the activity of ascorbic and sinapic acids and kaempferol 3-O-rutinoside was also studied. Among the compounds tested, sinapic acid showed the strongest antioxidant activity against both species.; We are grateful to Fundaçäo para a Ciência e a Tecnologia (POCI/AGR/57399/2004) for financial support of this work.; Peer reviewed

Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: Phytochemical characterization and antioxidant potential

Ferreres, Federico; Sousa, Carla; Valentão, Patricia; Seabra, Rosa M.; Pereira, José Alberto; Andrade, Paula B.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
Relevância na Pesquisa
96.38%
10 pages, 6 figures, 3 tables.; Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds were studied for their chemical composition and antioxidant capacity. Thirteen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-MS/MS-ESI and HPLC-DAD, respectively: two sinapoylgentiobiose isomers, three sinapoylglucose isomers, kaempferol-3-(sinapoyl)sophorotrioside-7-glucoside, sinapoylcholine, kaempferol-3,7-diglucoside-4′-(sinapoyl)glucoside, three disinapoylgentiobiose isomers, 1,2,2′-trisinapoylgentiobiose and 1,2-disinapoylglucose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids) were also identified and quantified by HPLC-UV. The aqueous extract of tronchuda cabbage seeds was investigated for its capacity to act as a scavenger of DPPH radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid), exhibiting antioxidant capacity in a concentration-dependent manner against all radicals. These results may be attributed to the high content of hydroxycinnamic derivatives and ascorbic acid.; The authors are grateful to Fundacäo para a Ciência e Tecnologia (POCI/AGR/57399/2004) for financial support of this work.; Peer reviewed

Screening of Antioxidant Compounds During Sprouting of Brassica oleracea L. var. costata DC

Sousa, Carla; Lopes, Graciliana; Pereira, David M.; Taveira, Marcos; Valentão, Patricia; Seabra, Rosa M.; Pereira, José Alberto; Baptista, Paula; Ferreres, Federico; Andrade, Paula B.
Fonte: Bentham Science Publishers Publicador: Bentham Science Publishers
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
Relevância na Pesquisa
96.38%
10 pages.; The changes in antioxidant compounds of Brassica oleracea L. var. costata DC seeds were monitored during the first twelve days of seedling development. Sprouts were screened at time intervals of two days for phenolic compounds and organic acids. The identified phenolic compounds included esters of sinapic acid with glucose, gentiobiose and kaempferol, as well as sinapoylcholine. The organic acids were oxalic, aconitic, citric, pyruvic, malic, shikimic, and fumaric acids. During germination, a depletion of phenolic compounds was observed, although no qualitative changes were seen. Among individual compounds, kaempferol, choline and glucose esters of sinapic acid showed a marked decrease between days two and six, whereas the changes in gentiobiose esters of sinapic acid were smaller. The total organic acids content increased rapidly during the first four days, with less significant variations thereafter. Malic acid, the major organic acid found in sprouts, greatly contributed to this result though oxalic, pyruvic, and fumaric acids also increased in the same manner. In contrast, aconitic, citric and shikimic acids showed decreases between days two and twelve of germination.; Peer reviewed

Influence of Two Fertilization Regimens on the Amounts of Organic Acids and Phenolic Compounds of Tronchuda Cabbage (Brassica oleracea L. Var. costata DC)

Sousa, Carla; Valentão, Patricia; Rangel, Joana; Lopes, Graciliana; Pereira, José Alberto; Ferreres, Federico; Seabra, Rosa M.; Andrade, Paula B.
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
Relevância na Pesquisa
96.38%
5 pages, 3 figures, 2 tables.; A phytochemical study was undertaken on tronchuda cabbage (Brassica oleracea L. var. costata DC) cultivated under conventional and organic practices and collected at different times. Six organic acids (aconitic, citric, ascorbic, malic, shikimic, and fumaric acids) were identified and quantified by HPLC-UV. Qualitative and quantitative differences were noted between internal and external leaves. Analysis of the phenolics of the internal leaves was achieved by HPLC-DAD, and the phenolic profile obtained was revealed to be distinct from that of the external leaves. By this means were identified and quantified 11 compounds: 3-p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-(caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(sinapoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(feruloyl)sophoroside-7-O-glucoside, kaempferol 3-O-sophoroside, two isomeric forms of 1,2-disinapoylgentiobiose, 1-sinapoyl-2-feruloylgentiobiose, 1,2,2‘-trisinapoylgentiobiose, and 1,2‘-disinapoyl-2-feruloylgentiobiose. In general, internal leaves exhibited more constant chemical profiles.; We are grateful to the Fundaçäo para a Ciência e Tecnologia (POCI/AGR/57399/2004) for financial support of this work.; Peer reviewed

Efeito inibitório na germinação, induzido pelo extrato de couve (Brassica oleracea L. var. acephala DC.); Inhibition effect of the germination, induced by extract of common kale (Brassica oleracea L. var. acephala DC)

Lucchesi, A.A.; Oliveira, R.F.
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/01/1988 POR
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Com a finalidade de se estudar as possíveis propriedades alelopáticas da couve (Brassica oleracea L. var. acephala DC), foi conduzido um bioensaio, em condições de laboratório, na E.S.A."Luiz de Queiroz", em Piracicaba, SP. Foram utilizadas sementes de tomate (Lycopersicon esculentum Mill. cv.Santa Cruz) colocadas para germinar em soluções com diferentes concentrações (0; 25; 50 e 100%) do extrato obtido da parte aérea (folhas) da couve. Constataram-se efeitos pronunciadamente inibitórios de germinação das sementes de tomate nas maiores concentrações do extrato de couve. As plântulas que germinaram, nas parcelas tratadas com o extrato, mostraram-se com o crescimento reduzido, morfo-fisiologicamente anormais, e com maior tempo para o início da germinação. Todas essas características foram mais pronunciadas nas parcelas com maiores concentrações do extrato.; With the purpose of studying the possible allelopathy characteristics of the common kale (Brassica oleracea L. var. acephala DC.), an experiment was conducted in laboratory conditions, at E.S.A. "Luiz de Queiroz", in Piracicaba, São Paulo State. Tomato seeds (Lycopersicon esculentum Mill. cv. Santa Cruz) were put to germinate in solutions with different concentrations (0; 25; 50 and 100%) of the extract obtained from the air part (leaves) of the common kale. Effects pronouncedly inhibitories of the germination of the tomato seeds were found in the largest concentrations of the common kale extract. The seedlings which germinated in the parcels treated with the extract presented reduced growth morpfo-physiologically abnormal and with larger time for the beginning of the germination. All these characteristics were more pronounced in the parcels with larger concentrations of the extract.