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Free amino acids of Tronchuda Cabbage (Brassica oleracea L. Var. costata DC): influence of leaf position (internal or external) and collection time

Oliveira, Andreia P.; Pereira, D.M.; Andrade, P.B.; Valentão, P.; Sousa, C.; Pereira, J.A.; Bento, Albino; Rodrigues, M.A.; Seabra, R.M.; Silva, B.M.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
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The free amino acid profile of 18 samples of tronchuda cabbage (Brassica oleracea L. var. costata DC) leaves, harvested at three different months, was determined by HPLC/UV−vis. The tronchuda cabbage leaves total free amino acid content varied from 3.3 to 14.4 g/kg fresh weight. Generally, arginine was the major compound, followed by proline, threonine, glutamine, cysteine, and glutamic acid. This study indicates that free amino acids are not similarly distributed: in external leaves, proline and arginine were the major free amino acids, while in internal ones, arginine was the main free amino acid, followed by threonine, glutamine, and cysteine. Significant differences were observed for valine, proline, arginine, leucine, cysteine, lysine, histidine, and tyrosine contents. The levels of some free amino acids were significantly affected by the collection period. In external leaves, this occurred with glutamic acid, serine, valine, leucine, cysteine, and ornithine contents, while in internal leaves, it occurred with aspartic acid, arginine, and total contents.

Phenolic compounds in external leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC)

Ferreres, F.; Valentão, P.; Llorach, R.; Pinheiro, C.; Cardoso, L.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
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Glycosylated kaempferol derivatives from the external leaves of tronchuda cabbage ( Brassica oleracea L. var. costataDC) characterized by reversed-phase HPLC-DAD-MS/MS-ESI were kaempferol 3- Osophorotrioside- 7-O-glucoside, kaempferol 3-O- (methoxycaffeoyl/caffeoyl)sophoroside-7- O-glucoside, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-sophorotrioside-7-O-sophoroside, kaempferol 3- O-sophoroside-7- O-sophoroside, kaempferol 3- O-tetraglucoside-7- O-sophoroside, kaempferol 3- O-(sinapoyl/caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(feruloyl/caffeoyl)sophoroside-7- O-glucoside, kaempferol 3-O-sophorotrioside, kaempferol 3-O-(sinapoyl)sophoroside, kaempferol 3-O- (feruloyl)sophorotrioside, kaempferol 3- O-(feruloyl)sophoroside, kaempferol 3- O-sophoroside, and kaempferol 3- O-glucoside. These acylated derivatives are reported for the first time in nature, with the exception of kaempferol 3- O-(sinapoyl)sophoroside. Quantification of the identified compounds was achieved by HPLC-DAD and carried out in samples cultivated under conventional or organic practices and collected at different times. In general, samples from organic production exhibited higher total phenolics content than those from conventional practices collected in the same period.

Influence of two fertilization regimes on the amounts of organic acids and phenolic compounds of tronchuda cabbage (Brassica oleracea L. var. costata DC)

Sousa, C.; Valentão, P.; Rangel, Joana; Lopes, Graciliana; Pereira, J.A.; Ferreres, F.; Seabra, R.M.; Andrade, P.B.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
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A phytochemical study was undertaken on tronchuda cabbage ( Brassica oleracea L. var. costata DC) cultivated under conventional and organic practices and collected at different times. Six organic acids (aconitic, citric, ascorbic, malic, shikimic, and fumaric acids) were identified and quantified by HPLC-UV. Qualitative and quantitative differences were noted between internal and external leaves. Analysis of the phenolics of the internal leaves was achieved by HPLC-DAD, and the phenolic profile obtained was revealed to be distinct from that of the external leaves. By this means were identified and quantified 11 compounds: 3- p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3- O-(caffeoyl)sophoroside-7- O-glucoside, kaempferol 3- O-(sinapoyl)sophoroside-7- Oglucoside, kaempferol 3- O-(feruloyl)sophoroside-7- O-glucoside, kaempferol 3- O-sophoroside, two isomeric forms of 1,2-disinapoylgentiobiose, 1-sinapoyl-2-feruloylgentiobiose, 1,2,2¢-trisinapoylgentiobiose, and 1,2¢-disinapoyl-2-feruloylgentiobiose. In general, internal leaves exhibited more constant chemical profiles.

Antioxidative properties of tronchuda cabbage (Brassica oleracea L. var. costata DC) external leaves against DPPH, superoxide radical, hydroxyl radical and hypochlorous acid

Vrchovska, V.; Sousa, C.; Valentão, P.; Ferreres, F.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
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The ability of the aqueous extract of tronchuda cabbage (Brassica oleracea L. var. costata DC) external leaves to act as a scavenger of DPPH- and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid) was investigated. A phytochemical study was also undertaken, and thirteen phenolic compounds and five organic acids were identified and quantified. Tronchuda cabbage extracts exhibited antioxidant capacity in a concentration-dependent manner in all assays, although some pro-oxidant effect was also noticed. The samples with higher phenolic and organic acid contents displayed the major antioxidant potentials.

Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phytochemical characterization and antioxidant potential

Ferreres, F.; Sousa, C.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Andrade, P.B.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
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Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds were studied for their chemical composition and antioxidant capacity. Thirteen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-MS/MS-ESI and HPLC-DAD, respectively: two sinapoylgentiobiose isomers, three sinapoylglucose isomers, kaempferol-3-(sinapoyl)sophorotrioside-7-glucoside, sinapoylcholine, kaempferol-3,7-diglucoside-40-(sinapoyl)glucoside, three disinapoylgentiobiose isomers, 1,2,20-trisinapoylgentiobiose and 1,2-disinapoylglucose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids) were also identified and quantified by HPLC-UV. The aqueous extract of tronchuda cabbage seeds was investigated for its capacity to act as a scavenger of DPPH- radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid), exhibiting antioxidant capacity in a concentration-dependent manner against all radicals. These results may be attributed to the high content of hydroxycinnamic derivatives and ascorbic acid.

Screening of antioxidant compounds during sprouting of Brassica oleracea L. var. costata DC

Sousa, C.; Lopes, Graciliana; Pereira, D.M.; Taveira, M.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Baptista, Paula; Ferreres, F.; Andrade, P.B.
Fonte: Bentham Science Publisher Publicador: Bentham Science Publisher
Tipo: Artigo de Revista Científica
ENG
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The changes in antioxidant compounds of Brassica oleracea L. var. costata DC seeds were monitored during the first twelve days of seedling development. Sprouts were screened at time intervals of two days for phenolic compounds and organic acids. The identified phenolic compounds included esters of sinapic acid with glucose, gentiobiose and kaempferol, as well as sinapoylcholine. The organic acids were oxalic, aconitic, citric, pyruvic, malic, shikimic, and fumaric acids. During germination, a depletion of phenolic compounds was observed, although no qualitative changes were seen. Among individual compounds, kaempferol, choline and glucose esters of sinapic acid showed a marked decrease between days two and six, whereas the changes in gentiobiose esters of sinapic acid were smaller. The total organic acids content increased rapidly during the first four days, with less significant variations thereafter. Malic acid, the major organic acid found in sprouts, greatly contributed to this result though oxalic, pyruvic, and fumaric acids also increased in the same manner. In contrast, aconitic, citric and shikimic acids showed decreases between days two and twelve of germination.

Multivariate analysis of of Tronchuda Cabbage (Brassica oleracea L. var. costata DC) phenolics: influence of fertilizers

Sousa, C.; Pereira, D.M.; Pereira, J.A.; Rodrigues, M.A.; Bento, Albino; Dopico-García, S.; Lopes, Graciliana; Valentão, P.; Seabra, R.M.; Andrade, P.B.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
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A field experiment was carried out to investigate the effect of fertilization level on the phenolic composition of tronchuda cabbage (Brassica oleracea L. var. costata DC) external and internal leaves. Eight different plots were constituted: a control without fertilization, one with organic matter, and six experiments with conventional fertilizers (nitrogen, boron, and sulfur, two levels each). The phenolic compounds were analyzed by reversed-phase HPLC-DAD. External and internal leaves revealed distinct qualitative composition. In the internal leaves were found 15 phenolics (5 kaempferol and 10 cinnamic acid derivatives), whereas the external leaves presented 3-p-coumaroylquinic acid and 13 kaempferol derivatives. Principal component analysis (PCA) was applied to assess the relationships between phenolic compounds, agronomical practices, and harvesting time. Samples obtained with conventional practices were quite effectively separated from organic samples, for both types of leaves. In general, samples developed without any fertilization presented the highest phenolics amounts: external and internal leaves contained 1.4- and 4.6-fold more phenolic compounds than the ones that received conventional fertilizer, respectively...

In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds.

Taveira, M.; Pereira, D.M.; Sousa, C.; Ferreres, F.; Andrade, P.B.; Martins, Anabela; Pereira, J.A.; Valentão, P.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
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In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH•. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits.

Screening of antioxidant phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC.

Ferreres, F.; Sousa, C.; Pereira, D.M.; Valentão, P.; Taveira, M.; Martins, Anabela; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
Fonte: Bentham Science Publicador: Bentham Science
Tipo: Artigo de Revista Científica
ENG
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The phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC were screened by HPLC-DAD-MS/MS. Thirty seven compounds were characterized, which included chlorogenic acids, flavonoids (the majority of them were hydroxycinnamic acid esters of kaempferol and quercetin glycosides) and hydroxycinnamic acyl glycosides (with predominance of synapoyl gentiobiosides). The antioxidant capacity of the shoots was assessed against DPPH radical and two reactive oxygen species (superoxide radical and hypochlorous acid). A strong concentrationdependent antioxidative capacity was verified in the DPPH and superoxide radicals assays, but a reduced effect was noticed against hypochlorous acid. The results obtained indicate that the in vitro production of B. oleracea var. costata shoots can become important in the obtention of a noticeable dietary source of compounds with health protective potential.

Brassica oleracea var. costata: comparative study on organic acids and biomass production with other cabbage varieties

Sousa, C.; Pereira, D.M.; Taveira, M.; Dopico-García, S.; Valentão, P.; Pereira, J.A.; Bento, Albino; Andrade, P.B.
Fonte: Wiley Publicador: Wiley
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
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BACKGROUND: A study was undertaken to evaluate the effect of agronomic practices, harvesting time and leaf age on the organic acid composition and biomass production of Brassica oleracea L. var. costata DC (tronchuda cabbage). Samples were cultivated under eight different fertilisation regimes (two levels each of nitrogen, boron and sulfur, an organic fertiliser and no fertiliser) and collected at three different times. RESULTS: Principal component analysis of the data indicated significant differences. Three principal components with an eigenvalue higher than one accounted for 79.0% of the total variance of the data set. Samples obtained with conventional fertilisation were characterised by the highest values of fresh weight. External leaves showed higher total organic acid and malic acid contents than internal leaves,while the latterwere characterised by higher proportions of citric acid. For consecutive harvests, total organic acid concentration decreased in both external and internal leaves. CONCLUSION: The use of a conventional fertilisation regime (nitrogen, boron or sulfur) improved the growth of B. oleracea var. costata without affecting its organic acid profile. However, for consecutive harvests, total organic acid concentration was observed to decrease independently of the agronomic practices tested. Leaf age influenced the quantitative composition of organic acids.

Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var. acephala).

Ferreres, F.; Fernandes, F.; Oliveira, J.M.A.; Valentão, P.; Pereira, J.A.; Andrade, P.B.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
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Phenolic and organic acid profiles of aqueous extracts from Pieris brassicae material and the host kale (Brassica oleracea L. var. acephala) leaves were determined by HPLC/UV–DAD/MSn-ESI and HPLC–UV, respectively. The identified phenolics included acylated and nonacylated flavonoid glycosides, hydroxycinnamic acyl gentiobiosides, and sulphate phenolics. Kale exhibited the highest content (11 g/kg lyophilized extract), while no phenolics were identified in the butterflies or exuviae. Nine different organic acids were characterized in the materials, with kale showing the highest amount (112 g/kg lyophilized extract). With the exception of the exuviae extract, the rest were screened for bioactivity. Using spectrophotometric microassays, all exhibited antiradical capacity against DPPH and NO in a concentrationdependent way, whereas only kale and excrement extracts were active against superoxide. All displayed activity on intestinal smooth muscle, albeit with distinct relaxation–contraction profiles. Larvae and butterfly extracts were more efficacious for intestinal relaxation than was kale extract, whereas excrement extract evoked only contractions, thus evidencing their different compositions. Collectively, these results show that P. brassicae sequesters and metabolizes kale’s phenolic compounds. Moreover...

Volatile constituents throughout Brassica oleracea L. var. acephala germination

Fernandes, F.; Pinho, P. Guedes de; Valentão, P.; Pereira, J.A.; Andrade, P.B.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
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In this work, the volatile composition of kale (Brassica oleracea L. var. acephala) and its variation during germination were monitored during the first 9 days of seedling development by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/ion trap-mass spectrometry (GC/IT-MS). Differences were found among the materials in the distinct analyzed periods. A total of 66 volatile compounds, distributed in several chemical classes, were determined: alcohols, carbonyl compounds (ketones, aldehydes, and esters), norisoprenoids, and terpenes, among others, sulfur compounds being the most abundant group in seeds and sprouts that exhibited allyl isothiocyanate as the major compound. Leaves of fully developed ground plant had the highest content of norisoprenoids, alcohols, and carbonyl compounds; in opposition, they showed lower levels of sulfur compounds, suggesting that these are important molecules for the development of kale, whereas the others are produced mainly during its growth.

Phenolics metabolism in insects: Pieris brassicae-Brassica oleracea var. costata ecological duo

Ferreres, F.; Fernandes, F.; Pereira, D.M.; Pereira, J.A.; Valentão, P.; Andrade, P.B.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Artigo de Revista Científica
ENG
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Changes in the phenolics composition of Pieris brassicae larvae fasted for distinct periods (1, 2, 4, 6, and 8 h) and their excrements and of Brassica oleracea L. var. costata DC leaves were determined by high-pressure liquid chromatography/UV-photo diode array detector/mass spectrometry- electrospray ionization. This is the first report following phenolics’ metabolism by P. brassicae through time. The results evidence that P. brassicae sequesters and metabolizes the phenolic compounds from the host plant. In a general way, deacylation was the main metabolic reaction that took place, but deglycosylation and sulfate conjugation reactions also occur. Additionally, several kaempferol derivatives containing rhamnose, which is not common in Brassica, were found in the host plant. Attending to the bioactivities recognized for the type of identified compounds, the different materials may constitute an interesting source of bioactive compounds, namely, of highly glycosylated and acylated kaempferol and quercetin derivatives, constituting an economic advantage for producers who have great losses caused by this pest. In addition, a deeper understanding of phenolics metabolism in insects was pursued.

Metabolic and bioactivity insights into Brassica oleracea var. acephala

Ferreres, F.; Fernandes, F.; Sousa, C.; Valentão, P.; Pereira, J.A.; Andrade, P.B.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
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Seeds of Brassica oleracea var. acephala (kale) were analyzed by HPLC/UV-PAD/MSn-ESI. Several phenolic acids and flavonol derivatives were identified. The seeds of this B. oleracea variety exhibited more flavonol derivatives than those of tronchuda cabbage (Brassica oleracea var. costata), also characterized in this paper. Quercetin and isorhamnetin derivatives were found only in kale seeds. Oxalic, aconitic, citric, pyruvic, malic, quinic, shikimic, and fumaric acids were the organic acids present in these matrices, malic acid being predominant in kale and citric acid in tronchuda cabbage seeds. Acetylcholinesterase (AChE) inhibitory activity was determined in aqueous extracts from both seeds. Kale leaves and butterflies, larvae, and excrements of Pieris brassicae reared on kale were also evaluated. Kale seeds were the most effective AChE inhibitor, followed by tronchuda cabbage seeds and kale leaves. With regard to P. brassicae material, excrements exhibited stronger inhibitory capacity. These results may be explained by the presence of sinapine, an analogue of acetylcholine, only in seed materials. A strong concentration-dependent antioxidant capacity against DPPH, nitric oxide, and superoxide radicals was observed for kale seeds.

Avaliação dos efeitos da radiação gama em vegetais da espécie Brassica olareaceae minimamente processados; EVALUATION OF THE EFFECTS OF GAMMA RADIATION ON THE VEGELABLES OF Brassica oleracea SPECIES MINIMALLY PROCESSED

Nunes, Thaise Cristine Fernandes
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 23/10/2009 PT
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O consumo de couve-manteiga (Brassica oleracea cv. acephala) e brócolis (Brassica oleracea L. var. italica) tem sido inversamente associado à morbidade e mortalidade causadas por doenças degenerativas. Estas espécies são altamente consumidas no Brasil, o que viabiliza o seu uso como minimamente processado (MP). A crescente preocupação mundial com a estocagem, qualidade nutricional e segurança microbiológica dos alimentos tem levado a muitos estudos visando à análise microbiológica, vitamínica e tempo de vida de prateleira. No intuito de melhorar a qualidade destes produtos, o processamento por radiação em baixas doses é eficiente na manutenção da qualidade do produto, em alguns casos não comprometendo seus valores nutricionais e sensoriais. O objetivo deste estudo foi avaliar a eficácia da radiação gama de 60Co com doses de 0, 1,0 e 1,5 kGy na redução da microbiota presente nestes vegetais, assim como analisar suas características nutricionais e sensoriais. A metodologia empregada neste trabalho foram as análises microbiológicas, análise colorimétrica, análise dos compostos fenólicos, análise antioxidante e análise sensorial. A análise microbiológica mostrou uma diminuição no desenvolvimento de populações de microrganismos aeróbios...

Tipificação de produtores, descrição de métodos de processamento mínimo e aspectos bromatológicos de couve (Brassica oleracea var. acephala L.) minimamente processada; Grower’s typing, methods description and bromatological aspects of freshcut kale (Brassica oleracea var. acephala L.)

Arteche, Irany Eugênia Boff
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
67.06%
As hortaliças minimamente processadas surgiram como uma interessante alternativa para o consumidor e segmentos de refeições coletivas, procurando otimizar recursos e resultados. No entanto, atualmente há uma grande preocupação por parte do consumidor com os aspectos nutricionais dos alimentos e de seus efeitos à saúde, resultando em uma busca por alimentos mais saudáveis e frescos, a exemplo das hortaliças minimamente processadas. Dentre as hortaliças folhosas, a couve (Brassica oleracea var. acephala L.), destaca-se pela rusticidade da cultura e valor nutricional, estando tradicionalmente na mesa dos brasileiros. O objetivo deste trabalho foi tipificar produtores e descrever diferentes sistemas de cultivos de couve, de onde se origina a matéria-prima a ser utilizada no processamento mínimo, analisar comparativamente diferentes fluxogramas de processamento mínimo que resultam em produtos disponíveis no mercado e comparar a composição centesimal de folhas de couve e couve minimamente processada. Os sistemas de cultivo foram estabelecidos a partir da experiência de cada produtor e apresentaram diferenças nos itinerários técnicos e no rendimento dos cultivos. Os fluxogramas de processamento mínimo, praticado por cada empresa...

Abundância de larvas e pupas de Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae) em Brassica oleracea l.var. Acephala d.c., mortalidade causada por parasitóides e biologia de Tetrastichus sokolowskii Kurdjmov, 1912 (Hymenoptera: Eulophidae)

Ferronatto, Edna Maria de Oliveira
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
POR
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Com o objetivo de estimar-se a densidade de Plutella xylostella (Linnaeus, 1758) numa cultura de Brassica oleracea L. var. acephala D C., cultivar "tronchuda portuguesa", foram realizadas amostras a intervalos regulares durante a estação 1980/81, na Estação Experimental Fitotécnica de Viamão, Distrito de Águas Claras, Município de Viamão, R.S. A área experimental consistiu de 500 plantas de couve; a unidade de amostra foi a folha. Na estação de 1980/81, em paralelo, estimou-se a mortalidade ocasionada por parasitóides aos estágios de larva e pupa. Acompanharam-se durante todo o período de ocorrência em campo as variações nas taxas de parasitismo em relação ao nível da população da praga. As três espécies predominantes de parasitóides foram Tetrastichus sokolowskii Kurdjmov, 1912. Apanteles piccotrichosus Blanchard, 1943 e Spilochalcis sp. aff. a fulvovariegata (Cameron, 1884). Em 1981/82, o parasitismo foi estimado independentemente da densidade do hospedeiro. Para as amostras, consideraram-se apenas os dois últimos estádios larvais. Nas condições em que foram realizados os experimentos, a ocorrência de P. xylostella e de seu complexo de parasitóides restringiu-se a um curto período do ano. Fora deste...

Identificação de marcadores moleculares ligados ao gene de resistência ao míldio, PpALG1 em Brassica oleracea var tronchuda

Alabaça, Cláudia S.
Fonte: Universidade do Algarve Publicador: Universidade do Algarve
Tipo: Dissertação de Mestrado
Publicado em //2009 POR
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Dissertação mest., Engenharia Biológica, Universidade do Algarve, 2009; O míldio das Crucíferas, causado pelo oomicete Hyaloperonospora Constant. parasitica (Pers. Ex Fr.) (Constantinescu e Fatehi, 2002), é uma doença que afecta a família Brassicaceae, sobretudo as espécies do género Brassica. Anteriormente, tinha sido identificada uma linha de Couve Algarvia (Brassica oleracea var tronchuda) resistente ao míldio na fase adulta cuja resistência se demonstrou ser controlada por um único gene dominante, designado PpALG1 (Monteiro et al., 2005). Este trabalho teve como objectivo a identificação de marcadores moleculares ligados ao gene PpALG1. Utilizando a estratégia de “Bulked Segregant Analysis” (BSA) para testar marcadores mapeados ao longo do mapa genético de B. oleracea concluiu-se que o gene PpALG1 se encontra ligado no mesmo grupo de ligamento (LG3) onde se encontra localizado o gene de resistência ao míldio, Pp523, identificado numa linha de brócolo. A estratégia de BSA foi novamente utilizada para testar marcadores seleccionados deste grupo, bem como para identificar novos marcadores RAPD ligados ao gene em estudo. Paralelamente foram testados marcadores STS provenientes de clones BAC de B. oleracea...

Factors Affecting the Glucosinolate Content of Kale (Brassica oleracea acephala Group)

Velasco Pazos, Pablo; Cartea González, María Elena; González, Carmen; Vilar Iglesias, Marta; Ordás Pérez, Amando
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artículo Formato: 4085 bytes; image/gif
ENG
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Kales (Brassica oleracea acephala group) are important vegetable crops in traditional farming systems in the Iberian Peninsula. They are grown throughout the year to harvest their leaves and flower buds. The glucosinolate content of kales is dependent upon the environmental factors, plant part examined, phenological stage of plant growth, and level of insect damage. The objectives of this study were to evaluate the changes in the total and individual glucosinolate concentrations during plant development and to determine if significant variation of glucosinolate levels can be explained by insect pests attack and other environmental factors in four locations in northwestern Spain. The total glucosinolate concentration in leaves of B. oleracea increased with plant age from seedling to early flowering stages. At that stage, the aliphatic glucosinolate content in leaves of B. oleracea declined drastically over time as the content in the flower buds increased. The highest contents of indolyl glucosinolate (glucobrassicin) and of the aromatic glucosinolate occurred in leaves harvested at the optimum consumption stage while flower buds contained the highest concentration of aliphatic glucosinolates, especially sinigrin. Sinigrin is reported to have anticarcinogenic properties. There appears to be a loss of total and individual glucosinolate concentrations related to pest attack. Leaves damaged by lepidopterous pests contained a lower total glucosinolate content (25.8 μmol g-1 dw) than undamaged leaves (41 μmol g-1 dw). The amounts of sinigrin...

Efeito inibitório na germinação, induzido pelo extrato de couve (Brassica oleracea L. var. acephala DC.); Inhibition effect of the germination, induced by extract of common kale (Brassica oleracea L. var. acephala DC)

Lucchesi, A.A.; Oliveira, R.F.
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/01/1988 POR
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Com a finalidade de se estudar as possíveis propriedades alelopáticas da couve (Brassica oleracea L. var. acephala DC), foi conduzido um bioensaio, em condições de laboratório, na E.S.A."Luiz de Queiroz", em Piracicaba, SP. Foram utilizadas sementes de tomate (Lycopersicon esculentum Mill. cv.Santa Cruz) colocadas para germinar em soluções com diferentes concentrações (0; 25; 50 e 100%) do extrato obtido da parte aérea (folhas) da couve. Constataram-se efeitos pronunciadamente inibitórios de germinação das sementes de tomate nas maiores concentrações do extrato de couve. As plântulas que germinaram, nas parcelas tratadas com o extrato, mostraram-se com o crescimento reduzido, morfo-fisiologicamente anormais, e com maior tempo para o início da germinação. Todas essas características foram mais pronunciadas nas parcelas com maiores concentrações do extrato.; With the purpose of studying the possible allelopathy characteristics of the common kale (Brassica oleracea L. var. acephala DC.), an experiment was conducted in laboratory conditions, at E.S.A. "Luiz de Queiroz", in Piracicaba, São Paulo State. Tomato seeds (Lycopersicon esculentum Mill. cv. Santa Cruz) were put to germinate in solutions with different concentrations (0; 25; 50 and 100%) of the extract obtained from the air part (leaves) of the common kale. Effects pronouncedly inhibitories of the germination of the tomato seeds were found in the largest concentrations of the common kale extract. The seedlings which germinated in the parcels treated with the extract presented reduced growth morpfo-physiologically abnormal and with larger time for the beginning of the germination. All these characteristics were more pronounced in the parcels with larger concentrations of the extract.