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Comparação de dois métodos de aplicação de fungicidas, irrigação por gotejamento e pulverização convencional no controle do mofo cinzento (Botrytis cinerea Pers.: Fr.) em vasos com plantas de lisianthus (Eustoma grandiflorum (Raf.) Shinn.)

Katz, Ieoschua; da Cunha, Antonio Ribeiro; Sousa, Antônio de Pádua; de Herdani, Egberto Egon
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 328-338
POR
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Plan disease control techniques are used through the irrigation water, which reduces the labor and it improves application uniformity with smaller contact of the operator with toxic products, lower environmental impact and lower production cost. In order to control Botrytis cinerea the lisianthus culture, this work aimed to evaluate two fungicide application methods with different treatments. The fungicides were: thiophanate methyl (50 g i.a. L-1), thiophanate methyl + chlorothalonil (50 g i.a. L-1 + 35 g i.a. L-1) and iprodione (50 g i.a. L-1). Number of lesions, number of diseased floral buttons and height of the lisianthus plants were evaluated. It was possible to deduce, that in the growth stage (number of lesions in the plant) as well as in the final stage (number of floral buttons) of the lisianthus culture, the most efficient treatments were 2 (thiophanate methyl + chlorothalonil) and 3 (iprodione). Considering that treatment 2 is a mixture of two fungicides, a systemic and a contact one, independently of application methods, the mixture increased efficiency in relation to treatment 1 (thiophanate methyl). Thus, chemigation was as efficient as spreading technique.

Efeito da radiação UV -B na interação Botrytis cinerea – clonostachys rosea em morangueiro e do ácido 4 - aminobenzóico no controle do patógeno em tabaco

Costa, Lúcio Bertoldo
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Tese de Doutorado Formato: viii, 87 f. : il. color. , gráfs, tabs.
POR
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Pós-graduação em Agronomia (Proteção de Plantas) - FCA; The incidence of ultraviolet (UV 100 to 400 nm) in the earth , especially UV - B radiation (280 - 320 nm) is being altered with climate change. The solar radiation is an import ant component for the development of microorganism in the environment, thus is important evaluate the tolerance of plant pathogens as well as the biocontrol agents to UV - B radiation. T he present study aimed to study the interaction of strawberry x Botrytis cinerea x Clonostachys rosea x UV - B radiation. There were significantly differences among the thirteen B. cinerea strains in relation to spore germination and sporulation on leaf disks after irradiation ranging from 2.9 to 8.9 KJ m - 2 . The relative germina tion ranged from 95 to 75% and the sporulation varied more than 100% among B. cinerea strains after exposure to 4 radiation of 6.4 KJ m - 2 . The LQC - 150 strain showed high germination and sporulation on leaf disk after irradiation and was selected as a toleran t strain. Survival curve of B. cinerea strain LQC - 150 showed lethal dose 50 (LD 50 ) of 6.2 KJ m - 2 . The sporulation of both fungi on leaf disks was inversely proportional to the dose of UV - B radiation, while inoculated alone. When confronted in the same leaf disk and not irradiated...

Avaliação do uso de quitosana e de fumigação para o controle de podridões em frutos da macieira (Malus domestica Borkh.) causadas por Penicillium expansum e Botrytis cinerea

Canaver, Bruno Sacconi
Fonte: Universidade Federal de Santa Catarina Publicador: Universidade Federal de Santa Catarina
Tipo: Dissertação de Mestrado Formato: xxviii, 122 p.| grafs., tabs.
POR
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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Biotecnologia, Florianópolis, 2010; Perdas substanciais de producao sao registradas em pos-colheita de macieira causadas principalmente pelos fungos Penicillium expansum e Botrytis cinerea. Os metodos usuais de controle envolvem a imersao de frutos em solucoes fungicidas e o uso da frigorificacao. Na busca por metodos alternativos, o objetivo deste trabalho foi avaliar os efeitos da quitosana e do vapor de acidos de cadeia curta, in vivo e in vitro, sobre os fungos P. expansum e B. cinerea. Nos ensaios in vivo, os frutos de macieira foram inoculados com uma suspensao de esporos de P. expansum ou B. cinerea antes (efeito curativo) ou apos (efeito preventivo) a imersao em suspensao de quitosana ou nas suas formulacoes. Os frutos tratados foram armazenados, no escuro, a 25+1C, sob alta umidade relativa, e avaliados periodicamente quanto a incidencia e a severidade das podridoes. Nos ensaios in vitro, avaliou-se o efeito de quitosana sobre a formacao de colonias de P. expansum, o crescimento micelial e a esporulacao de B. cinerea, bem como a germinacao de esporos e o comprimento do tubo germinativo de ambos. Os resultados indicaram que a imersao dos frutos de macieira em suspensoes de quitosana a 0...

Diversity of Botrytis cinerea from vineyards in the north west Iberian peninsula

Morales-Valle, H.; Paterson, R. R. M.; Venâncio, Armando; Lima, Nelson
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em /12/2011 ENG
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Botrytis cinerea is associated with a fungal gray rot in the concomitant regions of north west Spain and northern Portugal, where it is the most damaging pathogen and results in severe economic losses. Also, the physiological interactions of B. cinerea with Penicillium expansum are responsible for the production of geosmin, a volatile metabolite that transmit undesirable earthy odours to must and thus to wine. B. cinerea is not a homogeneous species and may be divided into several sub-species groupings. Some previous studies indicated two groups or cryptic species (I and II). Other work revealed that spore size and vegetative compatibility are characteristic features of these groups. In the present work a survey was taken of the population of B. cinerea from the above mentioned regions. The spore size and compatibility tests allowed characterisation of most isolates of B. cinerea into Group I or Group II taxa. Interestingly, some isolates could not be characterised according to their spore size and also presented ambiguous vegetative compatibility features. Furthermore, the influence of other factors on the spore size and grouping were studied. Grape variety, vineyard, country, sanitary state of the bunch and whether the isolates were obtained from the exterior or the interior of the bunch were compared with spore size and vegetative compatibility. Characterization of strains was affected by whether isolates were obtained from a particular grape variety. Also...

Interaction with Penicillium expansum enhances Botrytis cinerea growth in grape juice medium and prevents patulin accumulation in vitro

Morales-Valle, H.; Paterson, R. R. M.; Venâncio, Armando; Lima, Nelson
Fonte: Society for Applied Microbiology; Blackwell Publishing Inc. Publicador: Society for Applied Microbiology; Blackwell Publishing Inc.
Tipo: Artigo de Revista Científica
Publicado em //2013 ENG
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Interactions between fungi occur when they grow on the same host plant. This is the case of Botrytis cinerea and Penicillium expansum on grape. P. expansum is also responsible for production of the mycotoxin patulin. In this study, the influence of the interaction between both fungi on fungal growth parameters was studied as well as the effect on the accumulation of patulin by P. expansum. For that purpose, spores of B. cinerea and P. expansum were inoculated together (mixed inoculum), and the parameters growth rate, time for growth and patulin accumulation were assessed. The presence of P. expansum conidia shortened the time for growth of mixed inoculum colonies which, at the end of incubation, were B. cinerea-like. Although some P. expansum growth was observed in mixed inoculum colonies, very low levels of patulin were observed. In assays carried out in patulin-spiked medium, B. cinerea was capable to metabolize the mycotoxin. The capabilities of B. cinerea to shorten time for growth and prevent patulin accumulation are competing abilities that facilitate grape colonization. Significance and Impact of the Study: To our knowledge, this is the first study on the influence of fungal interactions between Botrytis cinerea and Penicillium expansum on growth parameters and patulin accumulation. The incidence of P. expansum in some wine regions is high...

Modelling the Climate in Unheated Tomato Greenhouses and predicting Botrytis cinerea infection

Baptista, Fátima
Fonte: Universidade de Évora Publicador: Universidade de Évora
Tipo: Tese de Doutorado Formato: 5497286 bytes; application/pdf
ENG
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Abstract Botrytis cinerea Pers.: Fr. is the causal agent of grey mould disease and is one of the most important diseases affecting tomato crops in unheated greenhouses. Ventilation is the technique used for environmental control in Mediterranean unheated greenhouses. Many growers tend to restrict nocturnal ventilation in order to increase air temperature, forgetting that humidity is a very important factor affecting plant development and most of all that high humidity is favourable to fungal disease development. Growers usually apply large quantities of chemical fungicides with disadvantages such as commercialization problems due to chemical residues on tomato fruits, high production costs, risk of fungicide resistance and negative environmental impacts. Nocturnal (or permanent) ventilation is an effective way to reduce high relative humidity inside greenhouses and could be a useful tool to minimise chemical use in unheated greenhouses. The main purpose of this research was to study the effect of nocturnal ventilation on B. cinerea occurrence in unheated tomato greenhouses and to develop a disease predictive model. Experiments were carried out at the Instituto Superior de Agronomia in Lisbon in two identical adjacent double-span greenhouses. The structural material was galvanized steel and the covering material was a three layer co-extruded film. Each greenhouse had a floor area of 182 m2...

Comparion of humidity conditions in unheated tomato greenhouses with different natural ventilation management and implications for climate and Botrytis cinerea control

Baptista, Fátima J.; Bailey, B.J.; Meneses, J.F.
Fonte: ISHS Publicador: ISHS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
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The objective of the research was to investigate the influence of nocturnal ventilation on the humidity conditions in unheated tomato greenhouses and the consequences for Botrytis cinerea control. Experiments were carried out at the Instituto Superior de Agronomia in Lisbon in two identical adjacent double-span greenhouses covered with three layer co-extruded film. The climate was controlled by natural ventilation, using continuous apertures located on the roof and side walls over the entire length of the greenhouses. Two different natural ventilation treatments were randomly assigned to the greenhouses. One treatment was permanent ventilation (PV), with the vents open during the day and night, while the other was classical ventilation (CV), in which the vents were open during the day and closed during the night. A spring tomato crop (Lycopersicon esculentum Miller), cultivar Zapata was grown directly in the soil between the end of February and the end of July. The growing technique was the usual for greenhouse tomatoes in Portugal. Trickle ferti-irrigation tubes were located between each two rows of plants. Climatic data were measured with three meteorological stations, one located in the centre of each greenhouse and one outside. All data were averaged and recorded on an hourly basis using two data logger systems. The number of leaflets with lesions caused by B. cinerea were counted and removed from the greenhouse from the randomly selected groups of plants. A significant reduction of air humidity occurred in the nocturnally ventilated greenhouse. Nocturnal or permanent ventilation was shown to give a great contribution to reducing disease severity on tomato leaves caused by B. cinerea. Nocturnal ventilation management is an environmental control technique which can be used as a prophylactic control measure...

Effect of nocturnal ventilation on the occurrence of Botrytis cinerea in Mediterranean unheated tomato greenhouses

Baptista, F.J.; Bailey, B.J.; Meneses, J.F.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
37.07%
Botrytis cinerea is the causal agent of grey mould disease which is one of the most important diseases affecting tomato crops in unheated greenhouses. Nocturnal ventilation is a technique that can be used to reduce relative humidity inside unheated greenhouses. The main objectives of this research were to investigate the effect of ventilation management on the environmental conditions and on the disease severity, to develop and validate a model which could predict disease severity and to present a warning system. Experiments were conducted in two plastic greenhouses. Two natural ventilation treatments were randomly assigned to the greenhouses. One was nocturnal ventilation (NV), with the vents open during the day and night, while the other was classical ventilation (CV), in which the vents were open during the day and closed during the night. A tomato crop was grown directly in the soil between the end of February and the end of July during two crop seasons. Climatic data were measured with three meteorological stations, averaged and recorded on an hourly basis. The number of diseased leaflets were counted and removed from the greenhouse. In the NV greenhouse a significant reduction of air humidity and disease appearance was observed. A warning system was developed and can be a useful tool for helping to decide on appropriate actions and the correct timing to avoid conditions that favour disease development. For a more practical application...

Reducing Botrytis cinerea incidence in unheated tomato greenhouses in real time using a wireless sensors network.

Baptista, F.J.; Neto, M.C.; Meneses, J.F.
Fonte: Universidade de Évora Publicador: Universidade de Évora
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
37.07%
The objective was to investigate the influence of ventilation on the humidity conditions in unheated tomato greenhouses and the consequences for Botrytis cinerea control and generate the necessary knowledge to build a warning system for this problem. Two different natural ventilation treatments were randomly assigned to the greenhouses. One treatment was permanent ventilation (PV), with the vents open during the day and night, while the other was classical ventilation (CV), in which the vents were open during the day and closed during the night. On a second stage and supported by a wireless network of air relative humidity and temperature sensors a rules based engine with defined setpoints will be developed to make available a early warning system via SMS, e- mail and Web. This will allow the growers to act only when needed, making possible to reduce chemical use, lowering both production costs and environmental impacts.

Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis

Duarte, José Manuel Martins Abrantes Teixeira
Fonte: Universidade Técnica de Lisboa Publicador: Universidade Técnica de Lisboa
Tipo: Dissertação de Mestrado
Publicado em //2008 ENG
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Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia; Botrytis cinerea [teleomorph: Botryotinia fuckeliana] is a heterothallic filamentous plant pathogenic Ascomycete with over two hundred different hosts, and its control at the crop level is still very difficult to achieve. Sexual reproduction as an active part in the fungus dispersal and variability has been underestimated until recent years. Research leading to more insight into the MAT locus and the fundamental principles of the mating process can prompt new strategies for B. cinerea control. During the B. cinerea infection process, phytotoxic proteins such as NEP1 are produced. Their function is yet to be unraveled, but it was postulated that they might play a role in speciation in the genus Botrytis. This report describes a mating type screening of a population of 80 field isolates of B. cinerea. Findings indicate a typical 1:1 Mendelian distribution of the MAT loci, and one strain with both MAT genes is reported. A scan of the MAT locus suggests the absence of the MAT1-1 locus in dual mating strains. Furthermore, Southern blotting demonstrates for the first time the possible presence of a complete MAT1-1 locus in a dual mating strain, instead of the predicted incomplete MAT1-1 locus (dMAT1). Besides the MAT loci...

Survival of Botrytis cinerea as mycelium in rose crop debris and as sclerotia in soil

Araújo,Alderi E; Maffia,Luiz A; Mizubuti,Eduardo S. G; Alfenas,Acelino C; Capdeville,Guy de; Grossi,José A. S
Fonte: Sociedade Brasileira de Fitopatologia Publicador: Sociedade Brasileira de Fitopatologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2005 EN
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Botrytis blight caused by Botrytis cinerea is an important disease of rose (Rosa hybrida) grown in greenhouses in Brazil. As little is known regarding the disease epidemiology under greenhouse conditions, pathogen survival in crop debris and as sclerotia was evaluated. Polyethylene bags with petals, leaves, or stem sections artificially infected with B. cinerea were mixed with crop debris in rose beds, in a commercial plastic greenhouse. High percentage of plant parts with sporulation was detected until 60 days, then sporulation decreased on petals after 120 days, and sharply decreased on stems or leaves after 90 days. Sporulation on petals continued for 360 days, but was not observed on stems after 150 days or leaves after 240 days. Although the fungus survived longer on petals, stems and leaves are also important inoculum sources because high amounts of both are deposited on beds during cultivation. Survival of sclerotia produced on PDA was also quantified. Sclerotia germination was greater than 75% in the initial 210 days and 50% until 360 days. Sclerotia weight gradually declined but they remained viable for 360 days. Sclerotia were produced on the buried petals, mainly after 90 days of burial, but not on leaves or stems. Germination of these sclerotia gradually decreased after 120 days...

Potencial de Pseudomonas sp. psicrotolerantes isoladas da Antártica no biocontrole de Botrytis cinerea.

BETIM, B. H.; SOUZA, W. R.; SANTOS, S. N.; PARMA, M. M.; KAVAMURA, V. N.; MELO, I. S. de
Fonte: In: CONGRESSO INTERINSTITUCIONAL DE INICIAÇÃO CIENTÍFICA, 5., 2011, Campinas. Anais... Campinas: Embrapa Monitoramento por Satélite, 2011. 1 CD ROM. Publicador: In: CONGRESSO INTERINSTITUCIONAL DE INICIAÇÃO CIENTÍFICA, 5., 2011, Campinas. Anais... Campinas: Embrapa Monitoramento por Satélite, 2011. 1 CD ROM.
Tipo: Artigo em anais de congresso (ALICE) Formato: Nº 11401.
PT_BR
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Resumo: A Antártica, por ser um ambiente extremo, comporta micro-organismos que podem ter características interessantes, como crescimento a baixas temperaturas (psicrotolerância), além da produção de compostos antimicrobianos. Como também a possibilidade de aplicação desses micro-organismos no controle de fitopatogenos que se desenvolveu em baixas temperaturas, como é o caso de Botrytis cinerea, que ataca dezenas de culturas agrícolas em zonas temperadas. O morango, Fragaria vesca, é comumente atacado por este fungo, causando a doença conhecida como podridão cinzenta, principalmente durante a pós-colheita. Desta forma, o objetivo deste trabalho foi prospectar bactérias isoladas da Antártica e avaliar o potencial de inibição deste fitopatogeno. Assim foram testadas 56 bactérias sendo que 42% apresentaram melhor crescimento a 4°C. 4 dessas pertencentes ao gênero Pseudomonas, sendo que a linhagem 44-4 (Pseudomonas Syringae), foi capaz de controlar o desenvolvimento do fungo em morango (4°C). Demonstra-se, portanto, a possibilidade de prospecção de bactérias Psicrofilicas com potencial de reduzir o crescimento de fungos a baixa temperatura. Ensaios estão sendo feitos para elucidação dos compostos químicos responsáveis por este efeito. Abstract: Antarctica...

Potencial de extratos à base de Calendula officinalis L. na indução de resistência e no efeito fungistático sobre Botrytis cinerea, in vitro

Fogolari, Hoilson
Fonte: Pato Branco Publicador: Pato Branco
Tipo: Dissertação de Mestrado
POR
Relevância na Pesquisa
37.34%
Several studies have demonstrated the potential of medicinal plants in the pathogens control, through its direct fungistatic action or its ability to induce plant defense, it suggesting the presence of molecules with characteristics elicitors. Three experiments were carried out on 2009 and 2010, in the Universidade Tecnológica Federal do Paraná, Dois Vizinhos city (PR – Brazil). The aim of this work was to evaluate prepared caledula (Calendula officinalis L.) potential for phytoalexins induction in the soybean cotyledons, resistance induction in the strawberry fruits and fungistatic effect of Botrytis cinerea in vitro. The experimental design was completely randomized with 15 treatments resulting from the combination of three forms of extraction (alcohol extract, infusion and maceration) and five concentrations (zero, 1.25, 2.5, 5 and 10%), with zero concentration distilled water in a factorial (3 x 5) with four replications the distilled water was used as control. The first experiment evaluated the potential fungistatic preparations in the B. cinerea control. The preparations with the different concentrations were put in Petri dishes with BDA culture media. After the media solidification, a hole of 8 mm was punched in the dishes Petri center and in this hole was introduced 2μL of spore B. cinerea suspension. The Petri dishes were kept in growth chamber for 7 days at 25°C. In the seventh day...

Characterization and protein fingerprinting of Botrytis cinerea isolates /

Aljourmi, Ismail.
Fonte: Brock University Publicador: Brock University
Tipo: Electronic Thesis or Dissertation
ENG
Relevância na Pesquisa
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Botrytis cinerea isolates collected from Niagara region were treated with different concentrations of the fiingicide, iprodione to test their sensitivity to this fungicide. These Botrytis cinerea isolates were divided into two groups according to their sensitivity to iprodione. Those isolates whose growth was inhibited by iprodione at concentrations < 2|i,g/nil were classified as sensitive isolates. Isolates that were able to show considerable growth at 2|j,g/ml iprodione were classified as resistant isolates. Resistant and sensitive isolates were compared for their morphological and growth characteristics, conidial germination, virulence on grape berries and protein banding profiles. The fungicide iprodione at a concentration of 2|xg/nil inhibited mycelial growth, sporulation and conidial germination of sensitive isolates but not those of resistant isolates. The inhibitory effect of the fungicide was greater on mycelial growth than on conidia germination of the sensitive isolates. Sensitive isolates produced no sclerotia whereas resistant isolates produced large number of sclerotia. The fungicide iprodione affected sclerotial production in the resistant isolates. The number of sclerotia was decreased by the increase of iprodione in the medium. Sporulation of resistant isolates was improved significantly in the presence of iprodione. The resistant isolates were as virulent as the sensitive isolates on grape berries. The sensitive and resistant isolates showed similar protein banding profiles in the absence of iprodione in polyacrylamide gel electrophoresis studies. Similar protein profiles were also observed when these isolates were grown in the presence of low iprodione concentration (0.5|ig/nil). However...

Carbon catabolite repression in plant pathogenic fungi: isolation and characterization of the Gibberella fujikuroi and Botrytis cinerea creA genes

Tudzynski, B.; Liu, S.; Kelly, J.
Fonte: Elsevier Science BV Publicador: Elsevier Science BV
Tipo: Artigo de Revista Científica
Publicado em //2000 EN
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The creA genes of two plant pathogenic fungi, the gibberellin-producing rice pathogen Gibberella fujikuroi and the gray mold Botrytis cinerea, were isolated and characterized. The deduced amino acid sequences of both glucose repressors are 64% identical to each other and 59% (G. fujikuroi) and 61% (B. cinerea) identical to the CreA protein of Aspergillus nidulans. The zinc finger regions of the Gibberella and Botrytis CreA proteins shared 98% identity with the corresponding zinc finger region of the A. nidulans protein, and studies by complementation of a creA null mutant of A. nidulans showed that the proteins are functional homologues of A. nidulans CreA. Northern blot analysis revealed that creA transcript levels are independent of the carbon source in both fungi.; Tudzynski, Bettina ; Liu, Songjie ; Kelly, Joan M.

Arabidopsis thaliana: A model host plant to study plant-pathogen interaction using Chilean field isolates of Botrytis cinerea

Gonzalez, Juan; Reyes, Francisca; Salas, Carlos; Santiago, Margarita; Codriansky, Yael; Colihueque, Nelson; Silva, Herman
Fonte: Sociedad de Biología de Chile Publicador: Sociedad de Biología de Chile
Tipo: Artigo de Revista Científica
EN
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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200004&lng=es&nrm=iso; One of the fungal pathogens that causes more agriculture damage is Botrytis cinerea. Botrytis is a constant threat to crops because the fungus infects a wide range of host species, both native and cultivated. Furthermore, Botrytis persists on plant debris in and on the soil. Some of the most serious diseases caused by Botrytis include gray mold on vegetables and fruits, such as grapes and strawberries. Botrytis also causes secondary soft rot of fruits and vegetables during storage, transit and at the market. In many plant-pathogen interactions, resistance often is associated with the deposition of callose, accumulation of autofluorescent compounds, the synthesis and accumulation of salicylic acid as well as pathogenesis-related proteins. Arabidopsis thaliana has been used as a plant model to study plant-pathogen interaction. The genome of Arabidopsis has been completely sequenced and this plant serves as a good genetic and molecular model. In this study, we demonstrate that Chilean field isolates infect Arabidopsis thaliana and that Arabidopsis subsequently activates several defense response mechanisms associated with a hypersensitive response. Furthermore...

A novel Botrytis species is associated with a newly emergent foliar disease in cultivated Hemerocallis

Grant-Downton, Robert T.; Terhem, Razak B.; Kapralov, Maxim V.; Mehdi, Saher; Rodriguez-Enriquez, M. Josefina; Gurr, Sarah J.; van Kan, Jan A. L.; Dewey, Frances M.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica Formato: 11 pages
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Foliar tissue samples of cultivated daylilies (Hemerocallis hybrids) showing the symptoms of a newly emergent foliar disease known as 'spring sickness' were investigated for associated fungi. The cause(s) of this disease remain obscure. We isolated repeatedly a fungal species which proved to be member of the genus Botrytis, based on immunological tests. DNA sequence analysis of these isolates, using several different phyogenetically informative genes, indicated that they represent a new Botrytis species, most closely related to B. elliptica (lily blight, fire blight) which is a major pathogen of cultivated Lilium. The distinction of the isolates was confirmed by morphological analysis of asexual sporulating cultures. Pathogenicity tests on Hemerocallis tissues in vitro demonstrated that this new species was able to induce lesions and rapid tissue necrosis. Based on this data, we infer that this new species, described here as B. deweyae, is likely to be an important contributor to the development of 'spring sickness' symptoms. Pathogenesis may be promoted by developmental and environmental factors that favour assault by this necrotrophic pathogen. The emergence of this disease is suggested to have been triggered by breeding-related changes in cultivated hybrids...

Aplicación de derivados de geranilfenoles y geranilhidroquinonas lineales en el control de Botrytis Cinerea y Erwinia Carotovora utilizando sistema encapsulado

Bay Chailan, Camila Francisca
Fonte: Universidad de Chile Publicador: Universidad de Chile
Tipo: Tesis
ES
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No autorizada por el autor para ser publicada a texto completo en portal de tesis hasta el 22/07/2018.; Ingeniera Civil en Biotecnología; Chile: Potencia Alimentaria y Forestal es el nuevo slogan adoptado por el país y simboliza el buen escenario del sector silvoagropecuario. Sin embargo, la planta y los frutos de la vid son atacados por el hongo patógeno Botrytis cinerea, produciendo grandes pérdidas productivas y económicas. Nuevas oportunidades de negocio se abren con el cultivo de flores de exportación. Para cuidar que las flores permanezcan en óptimas condiciones se debe combatir el principal patógeno que enferma a estas plantas, la bacteria Erwinia carotovora. Recientemente se ha demostrado que extractos de plantas, algas y esponjas marinas que contienen geranilfenoles y geranilhidroquinonas, poseen propiedades antifúngicas y antibacterianas. Debido a esto y los bajos rendimientos con que se obtienen desde la fuente natural es que se ha decidido sintetizar 6 de estos compuestos en el Laboratorio de Síntesis Orgánica de la UTFSM. Se estudió el efecto de los compuestos H1, M1, I1, I5, L1 y L2 sobre el crecimiento de Botrytis cinerea y Erwinia carotovora. Además, se planteó aumentar la solubilidad de los compuestos y su efectividad contra los patógenos...

Aislados chilenos de Botrytis cinerea resistentes a Iprodione : niveles de virulencia y caracterización del gen bos1

Araneda Rubio, María José
Fonte: Universidad de Chile Publicador: Universidad de Chile
Tipo: Tesis
ES
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Memoria para optar al título profesional de Ingeniero Agrónomo; Con el fin de evaluar la virulencia y características genéticas de aislados chilenos de Botrytis cinerea con distinta sensibilidad a iprodione, se seleccionaron nueve aislados altamente resistentes (EC50: 1,35 – 2,47 μg·mL-1) y cuatro aislados sensibles (EC50: 0,26 – 0,31 μg·mL-1). La virulencia se evaluó en plántulas de pepino (Cucumis sativus L.) mediante inoculación de discos de agar con micelio del hongo de tres días de edad y en bayas de vid (Vitis vinífera L.) Thompson Seedless con distinto grado de madurez (7°, 14° y 17° Brix), mediante inoculación de una suspensión conidial (equivalente a 2·105 conidias·mL-1). Posterior a la inoculación las plántulas de pepino se mantuvieron a 20-25°C y 100% de humedad relativa y las bayas de vid en cámaras de crecimiento a 20°C. La evaluación se realizó mediante medición del diámetro de la lesión en la zona de inoculación luego de 96 y 72 horas en hojas de pepino y bayas de vid, respectivamente. Los resultados obtenidos fueron sometidos a un ANDEVA simple y en las dos pruebas de virulencia realizadas los aislados de Botrytis cinerea resistentes y sensibles presentaron niveles de agresividad similares. La caracterización genética de los aislados se realizó amplificando mediante PCR el gen bos1 asociado a resistencia de Botrytis cinerea a dicarboximidas...

First report of Botrytis cinerea on Lilium polyphyllum, a critically endangered herb in Uttarakhand, India

Dhyani,A; Nautiyal,BP; Nautiyal,MC; Rivera,MC; Prasad,D; Singh,KP
Fonte: Phyton (Buenos Aires) Publicador: Phyton (Buenos Aires)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2012 EN
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Lilium polyphyllum is a critically endangered herb in the wilds of Uttarakhand, India. Gray mold appeared as a new disease on this species from July to August (2008-2009) causing death of inflorescences and shoots. Botrytis cinerea was consistently isolated on PDA, from infected inflorescences. Pathogenicity was established by inoculation of potted plants of L. polyphyllum in a glasshouse. Botrytis cinerea was recorded as a gray mold pathogen on L. polyphyllum for the first time in India, causing flower and shoot blight. Tis disease may seriously afect reproductive stages of this endangered host. To our knowledge, B. cinerea had not been reported previously on L. polyphyllum.