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Methods of quantification of training load in exercise performed at maximal lactate steady state

Nakamura, Fabio Yuzo; Soares-Caldeira, Lucio Flavio; Okuno, Nilo Massaru; Moreira, Alexandre; Simões, Herbert Gustavo
Fonte: UNIV ESTADUAL PAULISTA-UNESP, INST BIOCIENCIAS Publicador: UNIV ESTADUAL PAULISTA-UNESP, INST BIOCIENCIAS
Tipo: Artigo de Revista Científica
POR
Relevância na Pesquisa
56.21%
The aim of the present study was to compare and correlate training impulse (TRIMP) estimates proposed by Banister (TRIMP(Banister)), Stagno (TRIMP(Stagno)) and Manzi (TRIMP(Manzi)). The subjects were submitted to an incremental test on cycle ergometer with heart rate and blood lactate concentration measurements. In the second occasion, they performed 30 min. of exercise at the intensity corresponding to maximal lactate steady state, and TRIMP(Banister), TRIMP(Stagno) and TRIMP(Manzi) were calculated. The mean values of TRIMP(Banister) (56.5 +/- 8.2 u.a.) and TRIMP(Stagno) (51.2 +/- 12.4 u.a.) were not different (P > 0.05) and were highly correlated (r = 0.90). Besides this, they presented a good agreement level, which means low bias and relatively narrow limits of agreement. On the other hand, despite highly correlated (r = 0.93), TRIMP(Stagno) and TRIMP(Manzi) (73.4 +/- 17.6 u.a.) were different (P < 0.05), with low agreement level. The TRIMP(Banister) e TRIMP(Manzi) estimates were not different (P = 0.06) and were highly correlated (r = 0.82), but showed low agreement level. Thus, we concluded that the investigated TRIMP methods are not equivalent. In practical terms, it seems prudent monitor the training process assuming only one of the estimates.

Physiological and perceived exertion responses at intermittent critical power and intermittent maximal lactate steady state

Okuno, Nilo Massaru; Perandini, Luiz A. B.; Bishop, David; Simões, Herbert G.; Pereira, Gleber; Berthoin, Serge; Kokubun, Eduardo; Nakamura, Fabio Y.
Fonte: LIPPINCOTT WILLIAMS & WILKINS Publicador: LIPPINCOTT WILLIAMS & WILKINS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.21%
Okuno, NM, Perandini, LAB, Bishop, D, Simoes, HG, Pereira, G, Berthoin, S, Kokubun, E, and Nakamura, FY. Physiological and perceived exertion responses at intermittent critical power and intermittent maximal lactate steady state. J Strength Cond Res 25(7): 2053-2058, 2011-The aim of this study was to compare the power outputs of the intermittent critical power (CPi) with the intermittent maximal lactate steady state (MLSSi) and to compare the physiological and perceptual responses exercising at CPi and MLSSi. Ten subjects performed intermittent trials on a cycle ergometer to determine CPi and MLSSi using 30: 30 seconds of effort and pause. The oxygen uptake ((V) over dotO(2)), heart rate (HR), blood lactate concentration ([Lac]), and rating of perceived exertion (RPE) responses were compared during 30-minute cycling at CPi and MLSSi. The CPi (267 6 45 W) was similar to MLSSi (254 6 39 W), and they were correlated (r = 0.88; p<0.05). The (V) over dotO(2) and HR responses stabilized throughout exercising at CPi (2.52 +/- 0.52 L.min(-1); 156 +/- 8 b.min(-1)) and MLSSi (2.41 +/- 0.32 L.min(-1); 152 +/- 10 b.min(-1)). These physiological variables were similar between conditions. However, the [Lac] and RPE were higher from the middle to the end of exercise duration at CPi ([Lac] = 6.9 +/- 2.6 mM; RPE = 17.1 +/- 2.1 a.u.) compared to MLSSi ([Lac] = 5.1 +/- 0.9 mM; RPE = 15.7 +/- 1.8 a.u.). Therefore...

Acute Cardiorespiratory and Metabolic Responses During Resistance Exercise in the Lactate Threshold Intensity

de Sousa, N. M. F.; Magosso, R. F.; Pereira, G. B.; Souza, M. V. C.; Vieira, A.; Marine, D. A.; Perez, S. E. A.; Baldissera, V.
Fonte: GEORG THIEME VERLAG KG; STUTTGART Publicador: GEORG THIEME VERLAG KG; STUTTGART
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.24%
The aims were both to determine lactate and ventilatory threshold during incremental resistance training and to analyze the acute cardiorespiratory and metabolic responses during constant-load resistance exercise at lactate threshold (LT) intensity. Ten healthy men performed 2 protocols on leg press machine. The incremental test was performed to determine the lactate and ventilatory thresholds through an algorithmic adjustment method. After 48 h, a constant-load exercise at LT intensity was executed. The intensity of LT and ventilatory threshold was 27.1 +/- 3.7 and 30.3 +/- 7.9% of 1RM, respectively (P=0.142). During the constant-load resistance exercise, no significant variation was observed between set 9 and set 15 for blood lactate concentration (3.3 +/- 0.9 and 4.1 +/- 1.4 mmol.L-1, respectively. P=0.166) and BORG scale (11.5 +/- 2.9 and 13.0 +/- 3.5, respectively. P=0.783). No significant variation was observed between set 6 and set 15 for minute ventilation (19.4 +/- 4.9 and 22.4 +/- 5.5L. min(-1), respectively. P=0.091) and between S3 and S15 for VO2 (0.77 +/- 0.18 and 0.83 +/- 0.16L. min(-1), respectively. P=1.0). Constant-load resistance exercise at LT intensity corresponds to a steady state of ventilatory, cardio-metabolic parameters and ratings of perceived exertion.; Portuguese Foundation for Science and Technology (FCT) - Ministry of Science...

Lactato sanguíneo em atletas de judô : relato da experiencia de coleta durante combates sucessivos em uma competição oficial; Blood lactate in judo athletes : report of an experiment of sampling during successive fights in an official competition

Nunes, Alexandre Velly; Andrade, Rudnei de; Paiva, Claudio Roberto Escovar; Klemt, Ulf George
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
POR
Relevância na Pesquisa
56.35%
Este artigo e um relato de experiência que faz parte de uma investigação sobre a avaliação de atletas de judô de elite. Seu objetivo e comunicar a comunidade científica a metodologia utilizada, assim como descrever os procedimentos e as dificuldades encontradas na execução dessa metodologia. Nessa experiência, foram feitas 155 coletas de lactato sanguíneo em atletas de judô que participaram de uma competição internacional realizada em Porto Alegre, em julho de 1997. Os atletas da seleção gaúcha de judô, previamente selecionados (n = 21), submeteram-se a coleta de sangue do lóbulo da orelha antes e logo apos os combates. Alem desses, todos os atletas que participaram das lutas finais das respectivas categorias foram incentivados a submeter-se a coleta ao final do combate. Também foi verificada a FC dos atletas através de palpação radial em 15s, no momento da coleta de sangue. Utilizou-se o analisador de lactato portátil AccusportTM (Boehringer Mannheim Corp., Mannheim, Alemanha) com Test Strips BM Lactate e caneta com microlancetas descartáveis da mesma marca. Constatou-se que a utilização da metodologia e do equipamento acima descritos e apropriada para esse tipo de população e situação, e que os resultados encontrados podem contribuir para a avaliação da intensidade do esforço desses atletas.; This article reports an experience that is part of an investigation about elite judo athletes. The purpose of this study is to communicate...

Orange juice improved lipid profile and blood lactate of overweight middle-aged women subjected to aerobic training

Aptekmann, Nancy Preising; Cesar, Thais Borges
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 343-347
ENG
Relevância na Pesquisa
66.26%
Objective This study investigated how consumption of orange juice associated with aerobic training affected serum lipids and physical characteristics of overweight middle-aged womenMethods The experimental group consisted of 13 women who consumed 500 mL/d of orange juice and did 1 h aerobic training 3 times a week for 3 months The control group consisted of another 13 women who did the same aerobic training program but did not consume orange juiceResults At the end of the experiment the control group lost an average of 15% of fat mass (P < 0 05) and 25% of weight (P < 0 05) whereas the experimental group lost 11% of fat mass and 1 2% of weight (P < 0 05) Consumption of orange juice by the experimental group was associated with Increased dietary intake of vitamin C and folate by 126% and 61% respectively Serum LDL-C decreased 15% (P < 0 05) and HDL-C increased 18% (P < 0 05) in the experimental group but no significant change was observed in the control group Both groups improved the anaerobic threshold by 20% (P < 0 05) but blood lactate concentration decreased 27% in the experimental group compared to the 17% control group suggesting that experimental group has less muscle fatigue and better response to trainingConclusions The consumption of 500 mL/d of orange juice associated with aerobic training in overweight women decreased cardiovascular disease risk by reducing LDL-C levels and increasing HDL-C levels This association also decreased blood lactate concentration and increased anaerobic threshold showing some improvement in the physical performance (C) 2010 Elsevier B.V. All rights reserved

Blood lactate response and critical speed in swimmers aged 10-12 years of different standards

Denadai, B. S.; Greco, C. C.; Teixeira, M.
Fonte: E & Fn Spon Publicador: E & Fn Spon
Tipo: Artigo de Revista Científica Formato: 779-784
ENG
Relevância na Pesquisa
66.47%
It has previously been shown that measurement of the critical speed is a non-invasive method of estimating the blood lactate response during exercise. However, its validity in children has yet to be demonstrated. The aims of this study were: (1) to verify if the critical speed determined in accordance with the protocol of Wakayoshi et al. is a non-invasive means of estimating the swimming speed equivalent to a blood lactate concentration of 4 mmol . l(-1) in children aged 10-12 years; and (2) to establish whether standard of performance has an effect on its determination. Sixteen swimmers were divided into two groups: beginners and trained. They initially completed a protocol for determination of speed equivalent to a blood lactate concentration of 4 mmol . l(-1). Later, during training sessions, maximum efforts were swum over distances of 50, 100 and 200 m for the calculation of the critical speed. The speeds equivalent to a blood lactate concentration of 4 mmol . l(-1) (beginners = 0.82 +/- 0.09 m . s(-1), trained = 1.19 +/- 0.11 m . s(-1); mean +/- s) were significantly faster than the critical speeds (beginners = 0.78 +/- 0.25 m . s(-1), trained = 1.08 +/- 0.04 m . s(-1)) in both groups. There was a high correlation between speed at a blood lactate concentration of 4 mmol . l(-1) and the critical speed for the beginners (r = 0.96...

Determination of anaerobic threshold in rats using the lactate minimum test

Voltarelli, F.A.; Gobatto, C.A.; Mello, M.A.R. de
Fonte: Associação Brasileira de Divulgação Científica (ABRADIC) Publicador: Associação Brasileira de Divulgação Científica (ABRADIC)
Tipo: Artigo de Revista Científica Formato: 1389-1394
ENG
Relevância na Pesquisa
56.45%
The break point of the curve of blood lactate vs exercise load has been called anaerobic threshold (AT) and is considered to be an important indicator of endurance exercise capacity in human subjects. There are few studies of AT determination in animals. We describe a protocol for AT determination by the lactate minimum test in rats during swimming exercise. The test is based on the premise that during an incremental exercise test, and after a bout of maximal exercise, blood lactate decreases to a minimum and then increases again. This minimum value indicates the intensity of the AT. Adult male (90 days) Wistar rats adapted to swimming for 2 weeks were used. The initial state of lactic acidosis was obtained by making the animals jump into the water and swim while carrying a load equivalent to 50% of body weight for 6 min (30-s exercise interrupted by a 30-s rest). After a 9-min rest, blood was collected and the incremental swimming test was started. The test consisted of swimming while supporting loads of 4.5, 5.0, 5.5, 6.0 and 7.0% of body weight. Each exercise load lasted 5 min and was followed by a 30-s rest during which blood samples were taken. The blood lactate minimum was determined from a zero-gradient tangent to a spline function fitting the blood lactate vs workload curve. AT was estimated to be 4.95 ± 0.10% of body weight while interpolated blood lactate was 7.17 ± 0.16 mmol/l. These results suggest the application of AT determination in animal studies concerning metabolism during exercise.

Determination of the lactate threshold and maximal blood lactate steady state intensity in aged rats

Cunha, Rafael Rodrigues; de Carvalho Cunha, Verusca Najara; Segundo, Paulo Russo; Moreira, Sergio Rodrigues; Kokubun, Eduardo; Grubert Campbell, Carmen Silvia; de Oliveira, Ricardo Jaco; Simoes, Herbert Gustavo
Fonte: John Wiley & Sons Ltd Publicador: John Wiley & Sons Ltd
Tipo: Artigo de Revista Científica Formato: 351-357
ENG
Relevância na Pesquisa
56.3%
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); The reliability of the lactate threshold (LT) determined in aged rats and its, validity to identify an exercise intensity corresponding to the maximal blood lactate steady state (MLSS) were analyzed. Eighteen male aged Wistar rats (similar to 365 days) were submitted to two incremental swimming tests until exhaustion, consisting of an initial load corresponding to 1% of body mass (BM) and increments of 1% BM at each 3-min with blood lactate ([lac]) measurements. The LT was determined by visual inspection (LT(V)) as well by applying a polynomial function on the [lac]/workload ratio (LT(P)) by considering the vertices of the curve. For the MLSS, twelve animals were submitted, on different days, to 3-4 exercise sessions of 30-min with workload corresponding to 4, 5 or 6% BM. The MLSS was considered the highest exercise intensity at which the [lac] variation was not higher than 0.07 mM.min(-1) during the last 20-min. No differences were observed for the test-retest results (4.9 +/- 0.7 and 5.0 +/- 0.8 %BM for LTv; and 6.0 +/- 0.6 and 5.8 +/- 0.6 %BM for LTp) that did not differ from the MLSS (5.4 +/- 0.5 %BM). The LT identified for aged rats in swimming...

Maximal lactate steady state in running mice: Effect of exercise training

Ferreira, Julio C. B.; Rolim, Natale P. L.; Bartholomeu, Jan B.; Gobatto, Claudio A.; Kokubun, Eduardo; Brum, Patricia C.
Fonte: Blackwell Publishing Publicador: Blackwell Publishing
Tipo: Artigo de Revista Científica Formato: 760-765
ENG
Relevância na Pesquisa
56.37%
1. Maximal lactate steady state (MLSS) corresponds to the highest blood lactate concentration (MLSSc) and workload (MLSSw) that can be maintained over time without continual blood lactate accumulation and is considered an important marker of endurance exercise capacity. The present study was undertaken to determine MLSSw and MLSSc in running mice. In addition, we provide an exercise training protocol for mice based on MLSSw.2. Maximal lactate steady state was determined by blood sampling during multiple sessions of constant-load exercise varying from 9 to 21 m/min in adult male C57BL/6J mice. The constant-load test lasted at least 21 min. The blood lactate concentration was analysed at rest and then at 7 min intervals during exercise.3. The MLSSw was found to be 15.1 +/- 0.7 m/min and corresponded to 60 +/- 2% of maximal speed achieved during the incremental exercise testing. Intra- and interobserver variability of MLSSc showed reproducible findings. Exercise training was performed at MLSSw over a period of 8 weeks for 1 h/day and 5 days/week. Exercise training led to resting bradycardia (21%) and increased running performance (28%). of interest, the MLSSw of trained mice was significantly higher than that in sedentary littermates (19.0 +/- 0.5 vs 14.2 +/- 0.5 m/min; P = 0.05)...

Effect of exercise mode on the blood lactate removal during recovery of high-intensity exercise

Denadai, B. S.; Guglielmo, LGA; Denadai, MLDR
Fonte: Inst Sport Publicador: Inst Sport
Tipo: Artigo de Revista Científica Formato: 37-45
ENG
Relevância na Pesquisa
66.46%
The aim of this study was to determine the effect of exercise mode on the blood lactate removal during recovery of high-intensity exercise. Nine male individuals performed the following tests in order to determine the blood lactate removal: Running - 2x200 m, the subjects ran at their maximum capacity, and rested 2 min between each bout. Swimming - 2x50 m, the subjects swam at their maximum capacity, and rested 2 min between each bout. Each test was realized on different days with three recovery modes: passive (sitting down), swimming, or running. Recovery exercise intensity was corresponding to the aerobic threshold. All recovery activities lasted 30 min. The two forms of active recovery were initiated 2 min after the end of high-intensity exercise and lasted 15 min, and were followed by 13 min of seated rest. After 1,7, 12,17, and 30 min of the end of high-intensity exercise, blood samples (25 mu l) were collected in order to determine the blood lactate concentration. By linear regression, between the logarithm of lactate concentration and its respective time of recovery, the half-time of blood lactate removal (t1/2) was determined. Time of high-intensity exercise and the lactate concentration obtained in the 1(st) min of recovery were not different between running and swimming. Passive recovery (PR) following running (R-PR=25.5+/-4.3 min) showed a t1/2 significantly higher than PR after swimming (S-PR=18.6+/-4.3 min). The t1/2 of the sequences running-running (R-R=13.0 min)...

Effects of recovery type after a judo combat on blood lactate removal and on performance in an intermittent anaerobic task

Franchini, E.; Takito, M. Y.; Nakamura, F. Y.; Matsushigue, K. A.; Kiss, MAPD
Fonte: Edizioni Minerva Medica Publicador: Edizioni Minerva Medica
Tipo: Artigo de Revista Científica Formato: 424-431
ENG
Relevância na Pesquisa
56.32%
Aim. The objective of this study was to verify the effects of active (AR) and passive recovery (PR) after a judo match on blood lactate removal and on performance in an anaerobic intermittent task (4 bouts of upper body Wingate tests with 3-min interval between bouts; 4WT).Methods. The sample was constituted by 17 male judo players of different competitive levels: A) National (Brazil) and International medallists (n. 5). B) State (São Paulo) medallists (n. 7). Q City (São Paulo) medallists (n. 5). The subjects were submitted to: 1) a treadmill test for determination of VO2peak and velocity at anaerobic threshold (VAT); 2) body composition; 3) a 5-min judo combat, 15-min of AR or PR followed by 4WT.Results. The groups did not differ with respect to: body weight, VO2peak, VAT, body fat percentage, blood lactate after combats. No difference was observed in performance between AR and PR, despite a lower blood lactate after combat (10 and 15 min) during AR compared to PR. Groups A and B performed better in the high-intensity intermittent exercise compared to athletes with lower competitive level (C).Conclusion. The ability to maintain power output during intermittent anaerobic exercises can discriminate properly judo players of different levels. Lactate removal was improved with AR when compared to PR but AR did not improve performance in a subsequent intermittent anaerobic exercise.

Minimum blood lactate and muscle protein of rats during swimming exercise

Voltarelli, F. A.; Gobatto, C. A.; de Mello, M. A. R.
Fonte: Inst Sport Publicador: Inst Sport
Tipo: Artigo de Revista Científica Formato: 23-34
ENG
Relevância na Pesquisa
66.39%
Few studies dealing with effort intensity during swimming exercise in rats have been reported in the literature. Recently, with the use of the lactate minimum test (LMT), our group estimated the minimum blood lactate (MBL) of rats during swimming exercises. This information allowed accurate evaluation of the effort intensity developed by rats during swimming exercise. The present study was designed to evaluate the effects of swimming exercise sessions in below, equivalent and above intensities to MBL, on protein metabolism of rats. Adult (90 days) sedentary male Wistar rats were used in the present study. Mean values of MBL, in the present study, were obtained at blood concentration of 6.7 +/- 0.4 mmol/L with a load of 5% bw. The animals were sacrificed at rest (R) or immediately after a single swimming session (30 min) supporting loads below (3.5% bw), equivalent (5.0% bw) and high load (6.5% bw) to AT. Blood samples were collected each 5 min of exercise for lactate determination. Soleus muscle protein synthesis (amount of L-[C-14] fenil alanyn incorporation to protein) and breakdown (tyrosin release) rates were evaluated. Blood lactate concentrations (mmol/L) stabilized with the below (5.4 +/- 0.01) and equivalent (6.4 +/- 0.006) to MBL but increased...

The relationship between onset of blood lactate accumulation, critical velocity, and maximal lactate steady state in soccer players

Denadai, Benedito Sérgio; Gomide, Euripedes Barsanulfo Gonçalves; Greco, Camila Coelho
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 364-368
ENG
Relevância na Pesquisa
56.37%
The objective of this study was to analyze the validity of the velocity corresponding to the onset of blood lactate accumulation (OBLA) and critical velocity (CV) to determine the maximal lactate steady state (MLSS) in soccer players. Twelve male soccer players (21.5 ± 1.0 years) performed an incremental treadmill test for the determination of OBLA. The velocity corresponding to OBLA (3.5 mM of blood lactate) was determined through linear interpolation. The subjects returned to the laboratory on 7 occasions for the determination of MLSS and CV. The MLSS was determined from 5 treadmill runs of up to 30-minute duration and defined as the highest velocity at which blood lactate did not increase by more than 1 mM between minutes 10 and 30 of the constant velocity runs. The CV was determined by 2 maximal running efforts of 1,500 and 3,000 m performed on a 400-m running track. The CV was calculated as the slope of the linear regression of distance run versus time. Analysis of variance revealed no significant differences between OBLA (13.6 ± 1.4 km·h-1) and MLSS (13.1 ± 1.2 km·h-1) and between OBLA and CV (14.4 ± 1.1 km·h-1). The CV was significantly higher than the MLSS. There was a significant correlation between MLSS and OBLA (r = 0.80)...

Maximal lactate steady state in running rats

De Manchado, Fúlvia Barros; Gobatto, Claudio Alexandre; Contarteze, Ricardo Vinícius L.; Papoti, Marcelo; Rostom de Mello, Maria Alice
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 29-35
ENG
Relevância na Pesquisa
56.43%
The higher concentration during exercise at which lactate entry in blood equals its removal is known as maximal lactate steady state (MLSS) and is considered an important indicator of endurance exercise capacity. The aim of the present study was to determine MLSS in running rats. Adult male Wistar sedentary rats, which were selected and adapted to treadmill running for three weeks, were used. After becoming familiarized with treadmill running, the rats were submitted to five exercise tests at 15, 20, 25, 30 and 35 m/min velocities. The velocity sequence was distributed at random. Each test consisted of continuous running for 25 min at one velocity or until the exhaustion. Blood lactate was determined at rest and each 5 min of exercise to find the MLSS. The running rats presented MLSS at the 20 m/min velocity, with blood lactate of 3.9±1.1 mmol/L. At the 15 m/min velocity, the blood lactate also stabilized, but at a lower concentration (3.2±1.1 mmol/L). There was a progressive increase in blood lactate concentration at higher velocities, and some animals reached exhaustion between the 10 th and 25 th minute of exercise. These results indicate that the protocol of MLSS can be used for determination of the maximal aerobic intensity in running rats.

HYPERLACTEMIA INDUCTION MODES AFFECT THE LACTATE MINIMUM POWER AND PHYSIOLOGICAL RESPONSES IN CYCLING

Zagatto, Alessandro M.; Padulo, Johnny; Mueller, Paulo T. G.; Miyagi, Willian E.; Malta, Elvis S.; Papoti, Marcelo
Fonte: Lippincott Williams & Wilkins Publicador: Lippincott Williams & Wilkins
Tipo: Artigo de Revista Científica Formato: 2927-2934
ENG
Relevância na Pesquisa
56.39%
Zagatto, AM, Padulo, J, Muller, PTG, Miyagi, WE, Malta, ES, and Papoti, M. Hyperlactemia induction modes affect the lactate minimum power and physiological responses in cycling. J Strength Cond Res 28(10): 2927-2934, 2014The aim of this study was to verify the influence of hyperlactemia and blood acidosis induction on lactate minimum intensity (LMI). Twenty recreationally trained males who were experienced in cycling (15 cyclists and 5 triathletes) participated in this study. The athletes underwent 3 lactate minimum tests on an electromagnetic cycle ergometer. The hyperlactemia induction methods used were graded exercise test (GXT), Wingate test (WAnT), and 2 consecutive Wingate tests (2 x WAnTs). The LMI at 2 x WAnTs (200.3 +/- 25.8 W) was statistically higher than the LMI at GXT (187.3 +/- 31.9 W) and WAnT (189.8 +/- 26.0 W), with similar findings for blood lactate, oxygen uptake, and pulmonary ventilation at LMI. The venous pH after 2 x WAnTs was lower (7.04 +/- 0.24) than in (p <= 0.05) the GXT (7.19 +/- 0.05) and WAnT (7.19 +/- 0.05), whereas the blood lactate response was higher. In addition, similar findings were observed for bicarbonate concentration [HCO3] (2 x WAnTs lower than WAnT; 15.3 +/- 2.6 mmol center dot L-1 and 18.2 +/- 2.7 mmol center dot L(-)1...

Determination of anaerobic threshold in rats using the lactate minimum test

Voltarelli,F.A.; Gobatto,C.A.; Mello,M.A.R. de
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2002 EN
Relevância na Pesquisa
56.45%
The break point of the curve of blood lactate vs exercise load has been called anaerobic threshold (AT) and is considered to be an important indicator of endurance exercise capacity in human subjects. There are few studies of AT determination in animals. We describe a protocol for AT determination by the "lactate minimum test" in rats during swimming exercise. The test is based on the premise that during an incremental exercise test, and after a bout of maximal exercise, blood lactate decreases to a minimum and then increases again. This minimum value indicates the intensity of the AT. Adult male (90 days) Wistar rats adapted to swimming for 2 weeks were used. The initial state of lactic acidosis was obtained by making the animals jump into the water and swim while carrying a load equivalent to 50% of body weight for 6 min (30-s exercise interrupted by a 30-s rest). After a 9-min rest, blood was collected and the incremental swimming test was started. The test consisted of swimming while supporting loads of 4.5, 5.0, 5.5, 6.0 and 7.0% of body weight. Each exercise load lasted 5 min and was followed by a 30-s rest during which blood samples were taken. The blood lactate minimum was determined from a zero-gradient tangent to a spline function fitting the blood lactate vs workload curve. AT was estimated to be 4.95 ± 0.10% of body weight while interpolated blood lactate was 7.17 ± 0.16 mmol/l. These results suggest the application of AT determination in animal studies concerning metabolism during exercise.

Human lactate kinetics : training effects / by Jonathan David Buckley.

Buckley, Jonathan David
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado Formato: 480952 bytes; application/pdf
Publicado em //1997 EN
Relevância na Pesquisa
56.23%
This thesis examines the effects of endurance exercise training on whole body blood lactate removal, and the production and removal of blood lactate by skeletal muscle.; Thesis (Ph.D.)--University of Adelaide, Dept. of Physiology, 1997?; Bibliography: leaves 178-210.; xiii, 230 leaves : ill. ; 30 cm.

Determination of the lactate threshold and maximal blood lactate steady state intensity in aged rats

Cunha, Rafael Rodrigues; Cunha, Verusca Najara de Carvalho; Segundo, Paulo Russo; Moreira, Sérgio Rodrigues; Kokubun, Eduardo; Campbell, Carmen Sílvia Grubert; Oliveira, Ricardo Jacó de; Simões, Herbert Gustavo
Fonte: Universidade Católica de Brasília Publicador: Universidade Católica de Brasília
Tipo: Artigo de Revista Científica Formato: Texto
EN
Relevância na Pesquisa
56.3%
The reliability of the lactate threshold (LT) determined in aged rats and its validity to identify an exercise intensity corresponding to the maximal blood lactate steady state (MLSS) were analyzed. Eighteen male aged Wistar rats ( 365 days) were submitted to two incremental swimming tests until exhaustion, consisting of an initial load corresponding to 1% of body mass (BM) and increments of 1% BM at each 3-min with blood lactate ([lac]) measurements. The LTwas determined by visual inspection (LTV) as well by applying a polynomial function on the [lac]/workload ratio (LTP) by considering the vertices of the curve. For the MLSS, twelve animals were submitted, on different days, to 3–4 exercise sessions of 30-min with workload corresponding to 4, 5 or 6% BM. The MLSS was considered the highest exercise intensity at which the [lac] variation was not higher than 0.07 mM.min 1 during the last 20-min. No differences were observed for the test-retest results (4.9 0.7 and 5.0 0.8%BMfor LTv; and 6.0 0.6 and 5.8 0.6%BMfor LTp) that did not differ from the MLSS (5.4 0.5 %BM). The LT identified for aged rats in swimming, both by visual inspection and polynomial function, was reliable and did not differ from the MLSS.

Maximal lactate steady state estimated by different methods of anaerobic threshold; Máximo estado estável de lactato estimado por diferentes métodos de determinação

de Souza, Kristopher Mendes; Universidade Federal de Santa Catarina. Laboratório de Esforço Físico. Centro de Desportos. Florianópolis, SC. Brazil.; Grossl, Talita; Universidade Federal de Santa Catarina. Laboratório de Esforço Físico. Centro de De
Fonte: Universidade Federal de Santa Catarina. Florianópolis, SC. Brasil Publicador: Universidade Federal de Santa Catarina. Florianópolis, SC. Brasil
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; "Avaliado por Pares",; ; Avaliado por Pares; Descritiva correlacional Formato: application/pdf; application/pdf
Publicado em 30/04/2012 ENG
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56.35%
DOI: http://dx.doi.org/10.5007/1980-0037.2012v14n3p264The aim of this study was to compare anaerobic threshold (AT) as determined by four different methods with maximal lactate steady state (MLSS) in endurance runners. Nine moderately trained runners performed the following tests on different days: a maximal incremental exercise test to determine maximal oxygen uptake (VO2max), velocity at VO-2max (vVO2max), and blood lactate response; and two to five 30-min constant load tests to determine MLSS. Based on the incremental test, four methods of AT determination were used: AT1 – velocity at 3.5 mmol.L-1 blood lactate; AT2 – velocity corresponding to the minimum lactate-velocity equivalent plus 1.5 mmol.L-1; AT3 – velocity at Dmax; and AT4 – velocity before the second consecutive blood lactate increase greater than 0.5 mmol.L-1. There were no significant differences between MLSS and AT as determined by four different methods. However, the Bland-Altman analysis showed the extent of disagreement between variables when the subjects were analyzed individually. MLSS was significantly correlated with AT1 (r=0.68; p=0.04) and AT2 (r=0.79; p=0.01). Thus, although no significant differences were found between AT methods and MLSS, one should be cautious about using these methods interchangeably.; DOI: http://dx.doi.org/10.5007/1980-0037.2012v14n3p264O objetivo deste estudo foi determinar e comparar o limiar anaeróbio (LAn) obtido por quatro diferentes métodos com o máximo estado estável de lactato (MLSS) em corredores de endurance. Nove corredores moderadamente treinados realizaram...

Blood lactate concentrations and kinematic behavior of young runners in 50 and 100m sprints; Concentrações de lactato sangüíneo e o comportamento cinemático de corredores mirins em provas de 50 e 100m rasos

Dal Pupo, Juliano; Universidade Federal de Santa Catarina
Fonte: Universidade Federal de Santa Catarina. Florianópolis, SC. Brasil Publicador: Universidade Federal de Santa Catarina. Florianópolis, SC. Brasil
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; "Avaliado por Pares",; ; Avaliado por Pares; Pesquisa descritiva Formato: application/pdf; application/pdf
Publicado em 04/01/2008 POR; ENG
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http://dx.doi.org/10.5007/1980-0037.2008v10n4p393The purpose of this study was to verify the kinematic behavior of young runners during 50 and 100m sprints and their blood lactate concentration at the end of these sprints. Kinematic analysis was performed on the speed curve and some variables analyzed at the end of these runs. Fourteen individuals took part in this study, being 7 males (12.35 ± 0.83 years) and 7 females (12.61 ± 0.70 years). The speed curve was constructed using the panning method. The remaining kinematic variables were measured using two-dimensional videography with a Peak Motus video camera operating at 180 Hz. Blood samples were taken from the runners’ earlobes in order to assay lactate concentration. The results demonstrate that at both distances top speed is reached at 40m. This, however, corresponds to 80% of the distance of the 50m sprint, but just 40% of the 100m run. The significant differences in kinematic variables observed between the two sprints were: increased duration of the support phase in the males, decreased stride frequency among the females and lower average speed at the end of 100m in comparison to 50m, in all individuals. The highest blood lactate concentrations were observed after the 100m run. It was concluded that there was greater loss of performance during 100m...