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Establishment and characterization of human bladder cancer cell lines BexBra1, BexBra2, and BexBra4

PIANTINO, Camila B.; SOUSA-CANAVEZ, Juliana M.; SROUGI, Victor; SALVADORI, Fernanda; KATO, Raphael; AYRES, Pedro Paulo R.; Srougi, Miguel; CAMARA-LOPES, Luiz Heraldo; GATTAS, Gilka Jorge Figaro; FRIDMAN, Cintia; TOLEDO, Fernanda de; SANTANA, Isaque; LEITE
Fonte: SPRINGER Publicador: SPRINGER
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
66.25%
Bladder cancer (BC) is the fourth most common cancer in the USA. In Brazil, BC represents 3% of the total existing carcinomas in the population and represents the second highest incidence among urological tumors. The majority of bladder cancer cell lines available were derived from Caucasians and established in the seventies or eighties. Thus, neoplasia development in these cells likely occurred in environment conditions vastly different than today. In the present study, we report the establishment and characterization of three Brazilian bladder cancer cell lines (BexBra1, BexBra2, and BexBra4). These cell lines may be helpful for dissecting the genetic and epigenetic aspects that trigger the progression of BC. Moreover, the development of a Brazilian representative of the disease will allow us to investigate the potential inter-racial differences of malignancy-associated phenotypes in bladder cancer.

Avaliação analítica de potenciais biomarcadores para câncer de bexiga em urina; Analytical evaluation of potential biomarkers for bladder cancer in urine

Alberice, Juliana Vieira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 11/04/2014 PT
Relevância na Pesquisa
66.32%
O câncer de bexiga é uma neoplasia urogenital que acomete homens e mulheres, sendo que somente no Brasil 8.600 novos casos ao ano são diagnosticados. Cistoscopia transuretral é a conduta padrão no diagnóstico e acompanhamento do câncer de bexiga. Entretanto, tal procedimento é extremamente invasivo e doloroso além de ter elevado custo e não garantir todos os resultados. Assim, busca-se por marcadores moleculares que possam auxiliar no diagnóstico e progressão do câncer de bexiga, bem como diminuir a necessidade de exames invasivos no acompanhamento de pacientes tratados. Nesse sentido, a urina tem papel de destaque como fonte de biomarcadores devido principalmente ao seu caráter não invasivo.
Nesse trabalho foram utilizadas duas abordagens 'ômicas': proteômica e metabolômica, para a busca de biomarcadores em urina para o diagnóstico e prognóstico do câncer de bexiga, respectivamente. Com a abordagem proteômica buscou-se apenas por biomarcadores para o diagnóstico da doença e, utilizando as técnicas de eletroforese 2-DE, OFFGEL e MS, juntamente com análise estatística multivariada, foi possível identificar 32 proteínas que apresentam-se como potenciais marcadores para o câncer de bexiga. A abordagem metabolômica foi empregada para a busca de biomarcadores para reincidência e progressão da doença. As técnicas analíticas utilizadas nessa abordagem...

Low RKIP expression associates with poor prognosis in bladder cancer patients

Afonso, Julieta; Longatto-Filho, Adhemar; Martinho, Olga; Lobo, Francisco; Amaro, Teresina; Reis, Rui M.; Santos, Lúcio L.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 445-453
ENG
Relevância na Pesquisa
66.28%
Urothelial bladder cancer (UBC) is a heterogeneous type of disease. It is urgent to screen biomarkers of tumour aggressiveness in order to clarify the clinical behaviour and to personalize therapy in UBC patients. Raf kinase inhibitory protein (RKIP) is a metastasis suppressor, and its downregulation is associated with metastatic events in an increasing number of solid tumours. We evaluated the clinical and prognostic significance of RKIP expression in patients with high risk of progression UBC. Using immunohistochemistry, we determined RKIP expression levels in a series of 81 patients with high-grade pT1/pTis or muscle-invasive UBC. Staining of CD31 and D2-40 was used to assess blood and lymphatic vessels, in order to distinguish between blood and lymphatic vessel invasion (LVI). We found that 90 % of pT1/pTis tumours, 94 % of non-muscle invasive papillary tumours and 76 % of the cases without LVI occurrence expressed RKIP in >10 % of cells. In this group, we observed a subgroup of tumours (42 %) in which the tumour centre was significantly more intensely stained than the invasion front. This heterogeneous pattern was observed in 63 % of the cases with LVI. Low RKIP expression was associated with poorer 5-year disease-free and overall survival rates...

Cell cycle kinetics, apoptosis rates, DNA damage and TP53 gene expression in bladder cancer cells treated with allyl isothiocyanate (mustard essential oil)

Ventura Savio, Andre Luiz; Silva, Glenda Nicioli da; Camargo, Elaine Aparecida de; Favero Salvadori, Daisy Maria
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 40-46
ENG
Relevância na Pesquisa
66.21%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Allyl isothiocyanate (AITC) is present in plants of the cruciferous family and is abundant in mustard seed. Due to its high bioavailability in urine after ingestion, AITC has been considered a promising antineoplastic agent against bladder cancer. Because TP53 mutations are the most common alterations in bladder cancer cells and are frequently detected in in situ carcinomas, in this study, we investigated whether the AITC effects in bladder cancer cells are dependent on the TP53 status. Two bladder transitional carcinoma cell lines were used: RT4, with wild-type TP53; and T24, mutated TP53 gene. AITC was tested at concentrations of 0.005, 0.0625, 0.0725, 0.0825, 0.0925, 0.125 and 0.25 mu M in cytotoxicity, cell and clonogenic survival assays, comet and micronucleus assays and for its effects on cell cycle and apoptosis by flow cytometry and on TP53 gene expression. The data showed increased primary DNA damage in both cell lines; however, lower concentrations of AITC were able to induce genotoxicity in the mutant cells for the TP53 gene. Furthermore, the results demonstrated increased apoptosis and necrosis rates in the wild-type cells...

Clinical relevance of the sialyl-Tn antigen in bladder cancer; Relevância clínica do antigénio sialil-Tn no cancro da bexiga

Santos, Ana Margarida Gonçalves
Fonte: Universidade de Aveiro Publicador: Universidade de Aveiro
Tipo: Dissertação de Mestrado
ENG
Relevância na Pesquisa
66.3%
Approximately 50% of muscle invasive bladder cancers (MIBC) develop metastasis within 5 years after surgery, despite being subjected to pre- and post-surgery chemotherapy regimes. Thus, specific biomarkers to target aggressive cell phenotypes and direct molecular-based therapy are warranted. Recently, evidences have been presented that advanced stage bladder cancers express the cell-surface tumor-associated carbohydrate antigen sialyl-Tn (STn), which may be used to target aggressive bladder cancer cells. The STn antigen results from a premature stop in the O-glycosylation of cell-surface proteins and has been found to prevent immune recognition, contributing to avoid metastatic cell elimination, modulates the malignant phenotype and enhances the metastatic ability of cancer cells. In the present study, a series of 96 patients with bladder cancer of different stages was screened for STn expression and proliferation (over-expression of Ki-67) by immunohistochemistry. This showed an association between STn expression and tumor proliferation and invasion. STn expression was also observed in lymph node and distant metastases of STn positive tumors. The STn antigen was mainly detected in high-molecular weight glycoproteins (>250 kDa) and low-molecular weight proteins at 25...

Schistosomiasis mansoni of the bladder simulating bladder cancer: a case report

Casella,Mário Luis; Fanni,Victor Silvestre Soares; Verndl,Douglas Otto; Basso,Monique Camila; Mello,Luiz Figueiredo; Glina,Sidney
Fonte: Sociedade Brasileira de Medicina Tropical - SBMT Publicador: Sociedade Brasileira de Medicina Tropical - SBMT
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2009 EN
Relevância na Pesquisa
66.22%
The relationship between bladder tumors and Schistosoma haematobium is well known, but only sporadic cases of bladder infection due to Schistosoma mansoni have been reported. In this case, a 48-year-old woman with macroscopic hematuria, dysuria and a palpable abdominal mass was investigated. Ultrasound showed a large exophytic mass in the bladder. Transurethral resection of the bladder revealed viable eggs of Schistosoma mansoni. The patient was treated clinically with oxamniquine and surgery was performed to resect the large mass. This case shows that schistosomiasis Mansoni in the bladder can simulate bladder cancer.

In vitro and in vivo studies of pirarubicin-loaded SWNT for the treatment of bladder cancer

Chen,Gang; He,Yunfeng; Wu,Xiaohou; Zhang,Yao; Luo,Chunli; Jing,Peng
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2012 EN
Relevância na Pesquisa
66.28%
Intravesical chemotherapy is an important part of the treatment for superficial bladder cancer. However, the response to it is limited and its side effects are extensive. Functional single-walled carbon nanotubes (SWNT) have shown promise for tumor-targeted accumulation and low toxicity. In the present study, we performed in vivo and in vitro investigations to determine whether SWNT-based drug delivery could induce high tumor depression in rat bladder cancer and could decrease the side effects of pirarubicin (tetrahydropyranyl-adriamycin, THP). We modified SWNT with phospholipid-branched polyethylene glycol and constructed an SWNT-THP conjugate via a cleavable ester bond. The cytotoxicity of SWNT-THP against the human bladder cancer cell line BIU-87 was evaluated in vitro. Rat bladder cancer in situ models constructed by N-methyl-N-nitrosourea intravesical installation (1 g/L, 2 mg/rat once every 2 weeks for 8 weeks) were used for in vivo evaluation of the cytotoxicity of SWNT and SWNT-THP. Specific side effects in the THP group including urinary frequency (N = 12), macroscopic hematuria (N = 1), and vomiting (N = 7) were identified; however, no side effects were observed with SWNT-THP treatment. Flow cytometry was used to assess the cytotoxicity in vitro and in vivo. Results showed that SWNT alone did not yield significant tumor depression compared to saline (1.74 ± 0.56 and 1.23 ± 0.42%) in vitro. SWNT-THP exhibited higher tumor depression than THP-saline in vitro (74.35 ± 2.56 and 51.24 ± 1.45%) and in vivo (52.46 ± 2.41 and 96.85 ± 0.85%). The present findings indicate that SWNT delivery of THP for the treatment of bladder cancer leads to minimal side effects without loss of therapeutic efficacy. Therefore...

Presentation and outcome following radical cystectomy in hispanics with bladder cancer

Manoharan,M.; Ayyathurai,R.; Santos,R. De Los; Nieder,A. M.; Soloway,M. S.
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2008 EN
Relevância na Pesquisa
66.21%
OBJECTIVE: Significant racial and ethnic differences in the epidemiology of bladder cancer (BC) exist. Studies have shown African Americans to have lower incidence of bladder cancer than Caucasians, but higher incidence of invasive BC. Hispanics are the largest minority group in the United States. However, no reported studies on bladder cancer among Hispanics are available to date. As our center is in a unique position to study BC in Hispanic patients we were prompted to assess presentation and outcome of patients undergoing radical cystectomy (RC) for BC. MATERIALS AND METHODS: Between January 1992 and May 2006, 448 RC were performed. All relevant data were collected and entered into a database. Patients were categorized by ethnicity as Hispanic and non-Hispanic White. African-American and other minority groups were excluded because of the small number. Comparative analysis of Hispanic and non-Hispanic White patients was performed. RESULTS: 67 (17%) patients were Hispanic. Mean follow-up period was 41 (SD ± 40) months. Clinical and pathological data between these two groups were compared. Pre-cystectomy T stage was not significantly different between both groups. However, after RC incidence of ≤ T1 disease in Hispanics was lower (22%) than Caucasians (37%). This difference...

Laparoscopic partial cystectomy for urachal and bladder cancer

Colombo Jr.,Jose R.; Desai,Mihir; Canes,David; Frota,Rodrigo; Haber,Georges-Pascal; Moinzadeh,Alireza; Tuerk,Ingolf; Desai,Mahesh R.; Gill,Inderbir S.
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2008 EN
Relevância na Pesquisa
66.23%
PURPOSE: To report our initial experiences with laparoscopic partial cystectomy for urachal and bladder malignancy. MATERIALS AND METHODS: Between March 2002 and October 2004, laparoscopic partial cystectomy was performed in 6 cases at 3 institutions; 3 cases were urachal adenocarcinomas and the remaining 3 cases were bladder transitional cell carcinomas. All patients were male, with a median age of 55 years (45-72 years). Gross hematuria was the presenting symptom in all patients, and diagnosis was established with trans-urethral resection bladder tumor in 2 patients and by means of cystoscopic biopsy in the remaining 4 patients. Laparoscopic partial cystectomy was performed using the transperitoneal approach under cystoscopic guidance. In each case, the surgical specimen was removed intact entrapped in an impermeable bag. One patient with para-ureteral diverticulum transitional cell carcinoma required concomitant ureteral reimplantation. RESULTS: All six procedures were completed laparoscopically without open conversion. The median operating time was 110 minutes (90-220) with a median estimated blood loss of 70 mL (50-100). Frozen section evaluations of bladder margins were routinely obtained and were negative for cancer in all cases. The median hospital stay was 2.5 days (2-4) and the duration of catheterization was 7 days. There were no intraoperative or postoperative complications. Final histopathology confirmed urachal adenocarcinoma in 3 cases and bladder transitional cell carcinoma in 3 cases. At a median follow-up of 28.5 months (range: 26 to 44 months)...

Serum adenosine deaminase, catalase and carbonic anhydrase activities in patients with bladder cancer

Pirinççi,Necip; Geçit,Ilhan; Güneş,Mustafa; Yüksel,Mehmet Bilgehan; Kaba,Mehmet; Tanık,Serhat; Demir,Halit; Aslan,Mehmet
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2012 EN
Relevância na Pesquisa
66.25%
OBJECTIVES: The relationship between adenosine deaminase and various cancers has been investigated in several studies. However, serum adenosine deaminase activity and carbonic anhydrase and catalase activities in patients with bladder cancer have not previously been reported. Therefore, the aim of this study was to measure serum adenosine deaminase, carbonic anhydrase and catalase activities in patients with bladder cancer. MATERIALS AND METHODS: Forty patients with bladder cancer and 30 healthy controls were enrolled in the study. Serum adenosine deaminase, carbonic anhydrase and catalase activities were measured spectrophotometrically. RESULTS: Serum adenosine deaminase, carbonic anhydrase and catalase activities were significantly higher in patients with bladder cancer than controls (all significant, p<0.001). CONCLUSIONS: These markers might be a potentially important finding as an additional diagnostic biochemical tool for bladder cancer.

Prima-1 induces apoptosis in bladder cancer cell lines by activating p53

Piantino,Camila B.; Reis,Sabrina T.; Viana,Nayara I.; Silva,Iran A.; Morais,Denis R.; Antunes,Alberto A.; Dip,Nelson; Srougi,Miguel; Leite,Katia R.
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2013 EN
Relevância na Pesquisa
66.27%
OBJECTIVES: Bladder cancer represents 3% of all carcinomas in the Brazilian population and ranks second in incidence among urological tumors, after prostate cancer. The loss of p53 function is the main genetic alteration related to the development of high-grade muscle-invasive disease. Prima-1 is a small molecule that restores tumor suppressor function to mutant p53 and induces cancer cell death in various cancer types. Our aim was to investigate the ability of Prima-1 to induce apoptosis after DNA damage in bladder cancer cell lines. METHOD: The therapeutic effect of Prima-1 was studied in two bladder cancer cell lines: T24, which is characterized by a p53 mutation, and RT4, which is the wild-type for the p53 gene. Morphological features of apoptosis induced by p53, including mitochondrial membrane potential changes and the expression of thirteen genes involved in apoptosis, were assessed by microscopic observation and quantitative real-time PCR (qRT-PCR). RESULTS: Prima-1 was able to reactivate p53 function in the T24 (p53 mt) bladder cancer cell line and promote apoptosis via the induction of Bax and Puma expression, activation of the caspase cascade and disruption of the mitochondrial membrane in a BAK-independent manner. CONCLUSION: Prima-1 is able to restore the transcriptional activity of p53. Experimental studies in vivo may be conducted to test this molecule as a new therapeutic agent for urothelial carcinomas of the bladder...

Bladder Cancer: Sex Matters

Johnson, Aimee Michelle ; Reeder, Jay E. ; Mooney, Robert A.
Fonte: Universidade de Rochester Publicador: Universidade de Rochester
Tipo: Tese de Doutorado
ENG
Relevância na Pesquisa
66.38%
Thesis (Ph.D.)--University of Rochester. School of Medicine and Dentistry. Dept. of Pathology and Laboratory Medicine, 2008. ; The growing elderly population in the United States has led to a greater number of bladder cancer patients and bladder cancer deaths in the past several years. Mitogenic signaling pathways, specifically the receptor tyrosine kinase erbB2-mediated pathway, are misregulated in bladder cancer, leading to a greater replicative potential in cells that overexpress such mediators. Since erbB2 has been shown to be overexpressed in nearly 50% of bladder cancers, a mouse model of bladder cancer was created to overexpress erbB2 spatially and temporally. While erbB2 RNA expression was induced in this mouse model and papillary growths in the bladder were noted, erbB2 overexpression alone seemed insufficient for bladder tumorigenesis. It may be that additional mitogenic, cell cycle regulatory, and apoptotic pathways need to be involved. Several different animal models of bladder cancer have been utilized in the past four decades, although none of them have been able to reliably detect, measure, and monitor tumors in vivo, instead relying on serial sacrifice. To eliminate the need for such cumbersome experiments...

Androgen Receptor and Vitamin D Signaling in Bladder Cancer

Hsu, Jong-Wei ; Lee, Yi-Fen
Fonte: Universidade de Rochester Publicador: Universidade de Rochester
Tipo: Tese de Doutorado
ENG
Relevância na Pesquisa
66.26%
Thesis (Ph.D.)--University of Rochester. School of Medicine & Dentistry. Dept. of Pathology and Laboratory Medicine, 2011. ; Bladder cancer (BCa) is the fourth most common cancer in American men, and was estimated to be diagnosed in 70,530 people and cause 14,680 deaths in the USA in 2010. BCa is also the cancer which has the highest cost from diagnosis to death per patient. The cost in BCa is not proportionate to the incidence of BCa, which indicates that the efficacy of follow-up therapies need to be greatly improved. This thesis is focused on investigation of the impacts of androgen receptor (AR) and vitamin D signaling on BCa development and response to therapy, which would lead to the development of novel therapeutic approaches. Men have an approximately 3-fold higher risk of BCa than women in the USA, which indicates that sex hormones may be involved in BCa development. Previous studies suggested that both androgen and AR are involved in BCa development by using the general AR knockout (ARKO) mouse model. However, since 90% of BCa are derived from urothelium, it would be of interest to determine if urothelial AR plays a role in bladder tumorigenesis. To address this question, we generated the urothelial ARKO mouse model and applied two BCa mouse models: chemical N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN)-induced BCa and UPII-SV40T transgenic mouse BCa models. We found that knocking out urothelial AR decreased bladder tumorigenesis and tumor growth in the BBN-induced BCa mice. Consequently...

Gene products involved in metastasis of bladder cancer

Davies, B.R.
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
ENG
Relevância na Pesquisa
66.22%
Metastasis is usually responsible for mortality in patients suffering from muscle invasive bladder cancer. Whilst expression of a great number of genes and their protein products have been associated with metastasis and/or poor prognosis in bladder cancer, evidence that they actively drive the metastatic process, and hence make potentially good therapeutic targets, is often lacking. This is due to the limited number and application of effective animal models which reflect the pathogenesis of the human disease. In this review I will discuss the processes involved in metastasis, consider the established animal models of bladder cancer progression and metastasis, and review the evidence for a role of various gene products in this process. Consideration of clinical studies in conjunction with evidence from experimental animal models reveals that the tyrosine kinase receptor erbB1/EGFR, the calcium binding protein S100A4 and the the cell cycle arrest/apoptosis-inducing p53 protein are amongst the most promising targets for therapy against metastatic disease in patients with bladder cancer.

The S100 proteins for screening and prognostic grading of bladder cancer

Yao, R.; Davidson, D.D.; López Beltrán, A.; MacLennan, G.T.; Montironi, R.; Cheng, L.
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
ENG
Relevância na Pesquisa
66.23%
The S100 gene family, which is composed of at least 24 members carrying the Ca2+ binding EF-hand motif, has been implicated in both intracellular and extracellular functions, including enzyme activities, immune responses, cytoskeleton dynamics, Ca2+ homeostasis, cell growth and cell differentiation. Altered S100 protein levels are associated with a broad range of diseases, including cardiomyopathy, inflammatory and immune disorders, neurodegenerative disorders and cancer. Although the precise role of S100 protein in carcinogenesis is poorly understood, it seems that formation of homo- and hetero-dimers, binding of Ca2+ and interaction with effector molecules are essential for the development and progression of many cancers. Several studies have suggested that S100 proteins promote cancer progression and metastasis through cell survival and apoptosis pathways. In animal models of bladder cancer, several S100 proteins are differentially expressed in bladder tumors relative to normal urothelium. In human bladder cancer, overexpression of S100A4, S100A8 or S100A11 are associated with stage progression, invasion, metastasis and poor survival. This review summarizes these findings and evaluates their implications for human bladder cancer management

Estimating the burden of occupational bladder cancer in Ontario using the CAREX Canada database

Angeles, Joy
Fonte: Quens University Publicador: Quens University
Tipo: Tese de Doutorado Formato: 704807 bytes; application/pdf
EN; EN
Relevância na Pesquisa
66.34%
Objective: This study attempts to estimate the proportion of incident cases of bladder cancer in Ontario, Canada that is due to exposure to occupational carcinogens. Methods: The population attributable risk approach is used to estimate the proportion of bladder cancer in Ontario that is due to occupation. Risk ratios were obtained from a review of epidemiologic literature using a priori inclusion and exclusion criteria. Summary risk estimates for each bladder carcinogen included were calculated using RevMan 4.2. The CAREX Canada database provided Ontario-specific estimates of the proportion of workers exposed to bladder carcinogens. Results: In Ontario, the proportion of bladder cancer due to occupational exposure is approximately 5.6% (95% CI 0.2% to 14%). Based on the incident number of bladder cancer cases in 2001 in Ontario, it is estimated that approximately 52 new cases of bladder cancer were due to occupational exposure to polycyclic aromatic hydrocarbons (PAHs), diesel exhaust, aromatic amines and 2-naphthylamine. An alternate interpretation is if these occupational exposures were eliminated, 52 cases of bladder cancer per year in Ontario alone could be avoided. Conclusion and Recommendations: The current study advances our knowledge of the extent to which specific occupational bladder carcinogens contribute to the overall bladder cancer burden in Ontario. The current study highlights the utility of the CAREX Canada database in advancing current knowledge on the burden of occupational cancer in Ontario. The methods used to estimate the proportion of bladder cancer attributable to occupational exposure in Ontario may be replicated to estimate the proportion of cancer in Ontario that is due to occupational exposure.; Thesis (Master...

Predicting bladder cancer death amongst Australian Veterans

Plagakis, S.; Edwards, S.; O'Callaghan, M.; Foreman, D.
Fonte: Australian Military Medicine Association Publicador: Australian Military Medicine Association
Tipo: Artigo de Revista Científica
Publicado em //2015 EN
Relevância na Pesquisa
66.31%
Background: The purpose of this study was to compare the veteran and non-veteran cohorts of patients diagnosed with bladder cancer in order to determine if veterans have a worse clinical outcome, as has previously been demonstrated in prostate cancer. Methods: Using the Bladder Cancer Outcomes Database at the Repatriation General Hospital, South Australia, all bladder cancer cases between January 1984 and December 2011 were identified. This data was used to identify independent predictors of death in these populations and to contrast their five year bladder cancerspecific and overall survival. A subgroup of muscle-invasive bladder cancer was also analysed. There were a total of 1177 patients studied. Results: Overall, there was no significant difference in bladder cancer specific outcomes for veteran compared to non-veteran subjects. In both groups, the staging of disease at diagnosis was the strongest independent predictor of outcome, followed by the patient’s age at diagnosis. Veterans were generally older at diagnosis than non-veterans, and they did demonstrate worse all cause mortality outcomes. In the muscle invasive bladder cancer subgroup, outcomes were similar between veterans and non-veterans Conclusion: The independent predictors of outcome and bladder cancer specific survival rates in our South Australian cohort were similar to those described in the international literature and do not demonstrate poorer outcomes in our veteran population. All cause mortality was worse in the veteran population...

Laparoscopic partial cystectomy for urachal and bladder cancer

Colombo Jr., Jose R.; Desai, Mihir; Canes, David; Frota, Rodrigo; Haber, Georges-Pascal; Moinzadeh, Alireza; Tuerk, Ingolf; Desai, Mahesh R.; Gill, Inderbir S.
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/01/2008 ENG
Relevância na Pesquisa
66.23%
PURPOSE: To report our initial experiences with laparoscopic partial cystectomy for urachal and bladder malignancy. MATERIALS AND METHODS: Between March 2002 and October 2004, laparoscopic partial cystectomy was performed in 6 cases at 3 institutions; 3 cases were urachal adenocarcinomas and the remaining 3 cases were bladder transitional cell carcinomas. All patients were male, with a median age of 55 years (45-72 years). Gross hematuria was the presenting symptom in all patients, and diagnosis was established with trans-urethral resection bladder tumor in 2 patients and by means of cystoscopic biopsy in the remaining 4 patients. Laparoscopic partial cystectomy was performed using the transperitoneal approach under cystoscopic guidance. In each case, the surgical specimen was removed intact entrapped in an impermeable bag. One patient with para-ureteral diverticulum transitional cell carcinoma required concomitant ureteral reimplantation. RESULTS: All six procedures were completed laparoscopically without open conversion. The median operating time was 110 minutes (90-220) with a median estimated blood loss of 70 mL (50-100). Frozen section evaluations of bladder margins were routinely obtained and were negative for cancer in all cases. The median hospital stay was 2.5 days (2-4) and the duration of catheterization was 7 days. There were no intraoperative or postoperative complications. Final histopathology confirmed urachal adenocarcinoma in 3 cases and bladder transitional cell carcinoma in 3 cases. At a median follow-up of 28.5 months (range: 26 to 44 months)...

Serum adenosine deaminase, catalase and carbonic anhydrase activities in patients with bladder cancer

Pirinççi, Necip; Geçit, Ilhan; Güneş, Mustafa; Yüksel, Mehmet Bilgehan; Kaba, Mehmet; Tanık, Serhat; Demir, Halit; Aslan, Mehmet
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/12/2012 ENG
Relevância na Pesquisa
66.22%
OBJECTIVES: The relationship between adenosine deaminase and various cancers has been investigated in several studies. However, serum adenosine deaminase activity and carbonic anhydrase and catalase activities in patients with bladder cancer have not previously been reported. Therefore, the aim of this study was to measure serum adenosine deaminase, carbonic anhydrase and catalase activities in patients with bladder cancer. MATERIALS AND METHODS: Forty patients with bladder cancer and 30 healthy controls were enrolled in the study. Serum adenosine deaminase, carbonic anhydrase and catalase activities were measured spectrophotometrically. RESULTS: Serum adenosine deaminase, carbonic anhydrase and catalase activities were significantly higher in patients with bladder cancer than controls (all significant, p

Prima-1 induces apoptosis in bladder cancer cell lines by activating p53

Piantino, Camila B.; Reis, Sabrina T.; Viana, Nayara I.; Silva, Iran A.; Morais, Denis R.; Antunes, Alberto A.; Dip, Nelson; Srougi, Miguel; Leite, Katia R.
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/01/2013 ENG
Relevância na Pesquisa
66.27%
OBJECTIVES: Bladder cancer represents 3% of all carcinomas in the Brazilian population and ranks second in incidence among urological tumors, after prostate cancer. The loss of p53 function is the main genetic alteration related to the development of high-grade muscle-invasive disease. Prima-1 is a small molecule that restores tumor suppressor function to mutant p53 and induces cancer cell death in various cancer types. Our aim was to investigate the ability of Prima-1 to induce apoptosis after DNA damage in bladder cancer cell lines. METHOD: The therapeutic effect of Prima-1 was studied in two bladder cancer cell lines: T24, which is characterized by a p53 mutation, and RT4, which is the wild-type for the p53 gene. Morphological features of apoptosis induced by p53, including mitochondrial membrane potential changes and the expression of thirteen genes involved in apoptosis, were assessed by microscopic observation and quantitative real-time PCR (qRT-PCR). RESULTS: Prima-1 was able to reactivate p53 function in the T24 (p53 mt) bladder cancer cell line and promote apoptosis via the induction of Bax and Puma expression, activation of the caspase cascade and disruption of the mitochondrial membrane in a BAK-independent manner. CONCLUSION: Prima-1 is able to restore the transcriptional activity of p53. Experimental studies in vivo may be conducted to test this molecule as a new therapeutic agent for urothelial carcinomas of the bladder...