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Unhas humanas como marcadores biológicos de exposição ao flúor: correlação com a saliva da parótida e influência da idade; Human nails as biological markers of fluoride exposure: correlation to parotid ductal saliva and influence of age

Fukushima, Rejane
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 07/12/2007 PT
Relevância na Pesquisa
65.96%
Avaliou-se a influência da exposição ao flúor (F) através da água de beber, da velocidade de crescimento das unhas, da idade e do gênero na concentração deste elemento nas unhas das mãos e dos pés. Em adição, verificou-se a correlação entre as concentrações de F na saliva total, saliva do ducto da parótida e unhas das mãos e dos pés. Participaram do estudo 300 indivíduos, das faixas etárias de 3-7,14-20, 30-40 e 50-60 anos, residentes de cinco comunidades brasileiras, sendo duas no Estado de São Paulo (Pirajuí e Bauru, com água não fluoretada e 0,7 mgF/L na água de abastecimento, respectivamente) e três no Estado da Paraíba (Cajazeirinhas, Brejo dos Santos e Brejo das Freiras, com 0,2, 0,7 e 1,7 mgF/L na água de consumo, respectivamente). Foram coletadas duas ou três (apenas em Bauru) amostras de água de beber, além de duas amostras de: unhas dos pés, unhas das mãos, saliva total e saliva do ducto da parótida de cada indivíduo. O F nas amostras foi analisado com eletrodo íon-específico. Os dados obtidos foram analisados estatisticamente através de análise de variância e regressão linear (p<0,05). A exposição ao F através da água de beber, a velocidade de crescimento das unhas, a idade e o gênero influenciaram a concentração de F nas unhas das mãos e dos pés...

Desenvolvimento de metodologia analítica para a determinação de indicador biológico de exposição ao benzeno; Development of analytical methodology for the determination of biological marker of exposure to benzene

Coutrim, Mauricio Xavier
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 08/10/1998 PT
Relevância na Pesquisa
46.03%
Os limites de exposição ocupacional ao benzeno, um agente carcinogênico, vêm diminuindo drasticamente nos últimos anos. Por outro lado, a concentração de benzeno em ambientes não ocupacionais tem aumentado devido à emissão biogênica e antropogênica, como exaustão de motores a gasolina e fumaça de cigarro. Indicadores Biológicos de Exposição (IBE) são utilizados como ferramentas importantes na avaliação da exposição humana ao benzeno. Com a diminuição dos limites de exposição, se faz necessário o desenvolvimento de metodologias analíticas com sensibilidade adequada para a determinação de IBE em fluidos biológicos que se correlacionem com baixas concentrações de benzeno absorvido pelo organismo. A utilização do fenol urinário como IBE ao benzeno, embora reconhecida mundialmente, tem a desvantagem de não apresentar boa correlação com a concentração de benzeno ambiental quando esta é menor do que 10 ppm (32 mg/m3). Os ácidos trans,trans-mucônico e S-fenilmercaptúrico, metabólitos do benzeno encontrados na urina, estão entre os compostos mais estudados como IBE ao benzeno. Neste trabalho, o ácido trans,trans-mucônico foi determinado na urina de indivíduos expostos ao benzeno utilizando as técnicas de Eletroforese Capilar (CE) e HPLC...

Ácidos graxos como marcadores biológicos da ingestão de gorduras; Fatty acids as biological markers of fat intake

Vaz, Juliana dos Santos; Deboni, Fabíola; Azevedo, Mirela Jobim de; Gross, Jorge Luiz; Zelmanovitz, Themis
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
POR
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Os acidos graxos da dieta tem sido associados ao desenvolvimento de doencas cronicas. Os inqueritos alimentares, utilizados em estudos clinicos e epidemiologicos para estimativa da ingestao de nutrientes, apresentam limitacoes na coleta de informacoes. Nesse sentido, a utilizacao da composicao de acidos graxos do plasma e do tecido adiposo como marcadores do tipo de gordura alimentar pode fornecer uma medida mais acurada da ingestao de gorduras. Esta pesquisa tem como objetivo evidenciar aspectos metabolicos de alguns acidos graxos e o papel como marcadores da ingestao de gorduras, e apresentar as tecnicas analiticas empregadas na sua determinacao. A biopsia do tecido adiposo, com determinacao da composicao de acidos graxos, fornece uma informacao a longo prazo da ingestao de gorduras, enquanto que a avaliacao da composicao das fracoes lipidicas sericas representa a ingestao a curto e medio prazos. Os acidos graxos essenciais, os acidos graxos saturados com numero impar de carbonos (15:0 e 17:0) e os acidos graxos trans, por nao apresentarem sintese endogena, sao utilizados como marcadores biologicos da ingestao de gorduras ou de sua propria ingestao. As principais tecnicas utilizadas para a determinacao de acidos graxos sao a cromatografia gasosa e a cromatografia liquida de alta precisao. No presente momento...

Correlation of biological serum markers with the degree of hepatic fibrosis and necroinflammatory activity in hepatitis C and schistosomiasis patients

Morais,Clarice Neuenschwander Lins de; Carvalho,Bruno de Melo; Melo,Wlademir Gomes de; Melo,Fábio Lopes de; Lopes,Edmundo Pessoa de Almeida; Domingues,Ana Lúcia Coutinho; Jucá,Norma; Martins,João Roberto Maciel; Diniz,George Tadeu Nunes; Montenegro,Si
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/07/2010 EN
Relevância na Pesquisa
56.07%
Liver biopsy is the gold-standard method to stage fibrosis; however, it is an invasive procedure and is potentially dangerous. The main objective of this study was to evaluate biological markers, such as cytokines IL-13, IFN-γ, TNF-α and TGF-β, platelets, bilirubins (Bil), alanine aminotransferase (ALT) and aspartate aminotransferase (AST), total proteins, γ-glutamil transferase (γ-GT) and alkaline phosphatase (AP), that could be used to predict the severity of hepatic fibrosis in schistosomiasis and hepatitis C (HC) as isolated diseases or co-infections. The following patient groups were selected: HC (n = 39), HC/hepatosplenic schistosomiasis (HSS) (n = 19), HSS (n = 22) and a control group (n = 13). ANOVA and ROC curves were used for statistical analysis. P < 0.05 was considered significant. With HC patients we showed that TNF-α (p = 0.020) and AP (p = 0.005) could differentiate mild and severe fibrosis. With regard to necroinflammatory activity, AST (p = 0.002), γ-GT (p = 0.034) and AP (p = 0.001) were the best markers to differentiate mild and severe activity. In HC + HSS patients, total Bil (p = 0.008) was capable of differentiating between mild and severe fibrosis. In conclusion, our study was able to suggest biological markers that are non-invasive candidates to evaluate fibrosis and necroinflammatory activity in HC and HC + HSS.

Ultrasound versus biological markers in the evaluation of periportal fibrosis in human Schistosoma mansoni

Domingues,Ana Lúcia Coutinho; Medeiros,Tibério Batista de; Lopes,Edmundo Pessoa de Almeida
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2011 EN
Relevância na Pesquisa
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In this paper, the authors review the literature and share their experience of the principal biological markers of fibrosis for the evaluation of periportal fibrosis (PPF) caused by mansoni schistosomiasis. These biological markers are compared to diagnostic ultrasound (US) scans as means of grading PPF. We also review procollagen type I and III, collagen type IV, laminin, hyaluronic acid (HA), immunoglobulin G, platelets, aspartate aminotransferase to platelet ratio index (APRI) and gamma-glutamyl transpeptidase as markers of the disease. Although there are several good markers for evaluating PPF and portal hypertension, such as HA, platelets or APRI, none can yet replace US. These markers may, however, be used to identify patients at greater risk of developing advanced disease in endemic areas and determine who will need further care and US studies.

Markers and mediators of inflammatory response in infection and sepsis

Lobo,Suzana Margareth; Lobo,Francisco Ricardo Marques
Fonte: Associação de Medicina Intensiva Brasileira - AMIB Publicador: Associação de Medicina Intensiva Brasileira - AMIB
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2007 EN
Relevância na Pesquisa
46.12%
BACKGROUND AND OBJECTIVES: Sepsis is the expression of a complex network of mediators. Multiple organ dysfunction and septic shock indeed remain a major cause of death among ICU patients worldwide. Prompt recognition of both the diagnosis and the complicated evolution are essential, hence the importance of using biological markers. The main pro-inflammatory and anti-inflammatory cytokines as well as hundreds of others cellular markers, circulating bioactive molecules or coagulation products are potential biological markers that could help to characterize the presence of infection and sepsis. We aimed to review the main biological markers that could be used nowadays or possibly in the future, either in clinical or research fields. CONTENTS: A selective review of biologic markers of sepsis focusing on markers of the coagulation cascade, C-reactive protein and procalcitonin. CONCLUSIONS: Most of the available biological markers is still not a practical method to be used at the bed-side and is currently restricted to research works. Nowadays the determination of CRP or PCT serum levels can be of great help in the critically ill patient care along with the conventional parameters.

Biological markers in reproductive epidemiology: prospects and precautions.

Stein, Z; Hatch, M
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /10/1987 EN
Relevância na Pesquisa
46.15%
We begin by defining "biological markers" for the purposes of the present review, distinguishing markers from other types of information, such as subject reports or conventional clinical data. We find the distinctions to be hazy. Next, from the standpoint of epidemiologists, we set out circumstances in which exposure markers might be needed, suggesting requirements for useful markers. We give two instances (lead, PCB), drawn from studies of female reproduction, where the use of exposure markers is compared to environmental or anamnestic data. Effect markers are considered in turn. It is argued that their usefulness (if they are to be more informative than exposure markers) depends on their sensitivity and specificity in relation to the disease outcome. Also, their timeliness, and the use that can be made of the gain in time, for individuals and populations is discussed. In this context, we consider markers of events before and around fertilization; more specifically, we consider those events that precede the clinical marker of the first missed period. In returning to the potential uses of biological markers in discovering or interpreting female reproductive disorders that might be owed to environmental causes, we compare markers of the pre- and peri-implantation phases with markers of the postimplantation phase...

Validation of biological markers for quantitative risk assessment.

Schulte, P; Mazzuckelli, L F
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1991 EN
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46.09%
The evaluation of biological markers is recognized as necessary to the future of toxicology, epidemiology, and quantitative risk assessment. For biological markers to become widely accepted, their validity must be ascertained. This paper explores the range of considerations that compose the concept of validity as it applies to the evaluation of biological markers. Three broad categories of validity (measurement, internal study, and external) are discussed in the context of evaluating data for use in quantitative risk assessment. Particular attention is given to the importance of measurement validity in the consideration of whether to use biological markers in epidemiologic studies. The concepts developed in this presentation are applied to examples derived from the occupational environment. In the first example, measurement of bromine release as a marker of ethylene dibromide toxicity is shown to be of limited use in constructing an accurate quantitative assessment of the risk of developing cancer as a result of long-term, low-level exposure. This example is compared to data obtained from studies of ethylene oxide, in which hemoglobin alkylation is shown to be a valid marker of both exposure and effect.

Clinical application of biological markers for treatments of resectable non-small-cell lung cancers

Huang, C; Liu, D; Masuya, D; Nakashima, T; Kameyama, K; Ishikawa, S; Ueno, M; Haba, R; Yokomise, H
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
EN
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46%
We performed a clinical study to identify biological markers useful for the treatment of resectable non-small-cell lung cancers (NSCLCs). In all, 173 patients were studied. By immunohistochemistry, we evaluated the Ki-67 proliferation index, tumour vascularity, thymidylate synthase (TS), vascular endothelial growth factor (VEGF)-A, VEGF-C, and E (epithelial)-cadherin. Concerning the survival of NSCLC patients, tumour vascularity (P<0.01), VEGF-A status (P=0.03), VEGF-C status (P=0.03), and E-cadherin status (P=0.03) were significant prognostic factors in patients with stage I NSCLCs. The Ki-67 proliferation index (P=0.02) and TS status (P<0.01) were significant prognostic factors in patients with stage II–III NSCLCs. In patients with stage II–III NSCLCs, furthermore, the survival of UFT (a combination of tegafur and uracil)-treated patients with TS-negative tumours was significantly better than those of any other patients. Biological markers associated with tumour angiogenesis or metastasis are useful for the detection of aggressive tumours among early-stage NSCLCs. Postoperative chemotherapy might be necessary in such tumours even in stage I. In contrast, tumour proliferation rate and TS status are useful markers for identifying less aggressive tumours in locally advanced NSCLCs. Thymidylate synthase expression is also a useful marker to evaluate responsiveness of UFT-based chemotherapy for these tumours.

Pathogenetic Significance of Biological Markers of Ventilator-Associated Lung Injury in Experimental and Clinical Studies*

Frank, James A.; Parsons, Polly E.; Matthay, Michael A.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /12/2006 EN
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46.11%
For patients with acute lung injury, positive pressure mechanical ventilation is life saving. However, considerable experimental and clinical data have demonstrated that how clinicians set the tidal volume, positive end-expiratory pressure, and plateau airway pressure influences lung injury severity and patient outcomes including mortality. In order to better identify ventilator-associated lung injury (VALI), clinical investigators have sought to measure blood-borne and airspace biological markers of VALI. At the same time, several laboratory-based studies have focused on biological markers of inflammation and organ injury in experimental models in order to clarify the mechanisms of ventilator-induced lung injury (VILI) and VALI. This review summarizes data on biological markers of VALI and VILI from both clinical and experimental studies with an emphasis on markers identified in patients and in the experimental setting. This analysis suggests that measurement of some of these biological markers may be of value in diagnosing VALI and in understanding its pathogenesis.

Biological Markers in DCIS and Risk of Breast Recurrence: A Systematic Review

Lari, Sara A.; Kuerer, Henry M.
Fonte: Ivyspring International Publisher Publicador: Ivyspring International Publisher
Tipo: Artigo de Revista Científica
Publicado em 01/05/2011 EN
Relevância na Pesquisa
46.15%
Understanding of the biology and clinical behavior of ductal carcinoma in situ (DCIS) is currently inadequate. The aim of this comprehensive review was to identify important molecular biological markers associated with DCIS and candidate markers associated with increased risk of ipsilateral recurrence after diagnosis of DCIS. A comprehensive systematic review was performed to identify studies published in the past 10 years that investigated biological markers in DCIS. To be included in this review, studies that investigated the rate of biological expression of markers had to report on at least 30 patients; studies that analyzed the recurrence risk associated with biomarker expression had to report on at least 50 patients. There were 6,252 patients altogether in our review. Biological markers evaluated included steroid receptors, proliferation markers, cell cycle regulation and apoptotic markers, angiogenesis-related proteins, epidermal growth factor receptor family receptors, extracellular matrix-related proteins, and COX-2. Although the studies in this review provide valuable preliminary information regarding the expression and prognostic significance of biomarkers in DCIS, common limitations of published studies (case-series, cohort...

Biological Markers Predictive of Invasive Recurrence in DCIS

Nofech-Mozes, Sharon; Spayne, Jacqueline; Rakovitch, Eileen; Kahn, Harriette J; Seth, Arun; Pignol, Jean-Phillippe; Lickley, Lavina; Paszat, Lawrence; Hanna, Wedad
Fonte: Libertas Academica Publicador: Libertas Academica
Tipo: Artigo de Revista Científica
Publicado em 22/01/2008 EN
Relevância na Pesquisa
46%
DCIS is a heterogeneous group of non-invasive cancers of the breast characterized by various degrees of differentiation and unpredictable propensity for transformation into invasive carcinoma. We examined the expression and prognostic value of 9 biological markers with a potential role in tumor progression in 133 patients with pure DCIS treated with breast conserving surgery alone, between 1982–2000. Histology was reviewed and immunohistochemical staining was performed. Pearson correlation coefficient was used to determine the associations between markers and histopathological features. Univariate and multivariate analysis examined associations between time to recurrence and clinicopathologic features and biological markers.

Impact of Chronic Lead Exposure on Selected Biological Markers

Jangid, Ambica P.; John, P. J.; Yadav, D.; Mishra, Sandhya; Sharma, Praveen
Fonte: Springer-Verlag Publicador: Springer-Verlag
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.04%
Lead poisoning remains a major problem in India due to the lack of awareness of its ill effects among the clinical community. Blood lead, δ-aminolevulinic acid dehydratase (δ-ALAD) and zinc protoporphyrin (ZPP) concentrations are widely used as biomarkers for lead toxicity The present study was designed to determine the impact of chronic lead exposure on selected biological markers. A total of 250 subjects, of both sexes, ranging in age from 20 to 70 years, were recruited. On the basis of BLLs, the subjects were categorized into four groups: Group A (BLL: 0–10 μg/dl), Group B (BLL: 10–20 μg/dl). Group C (BLL: 20–30 μg/dl) and Group D (BLL: 30–40 μg/dl) having BLLs of 3.60 ± 2.71 μg/dl, 15.21 ± 2.65 μg/dl, 26.82 ± 2.53 μg/dl and 36.38 ± 2.83 μg/dl, respectively. Significant changes in biological markers due to elevated BLLs were noted. The relation of BLL and biological markers to demographic characteristics such as sex, habits, diet and substances abuse (smoking effect) were also studied in the present investigation. Males, urban population, non-vegetarians, and smokers had higher blood lead levels. δ-ALAD activity was found to be significantly lower with increased BLL (P < 0.001), while the ZPP level was significantly higher with increased BLL (P < 0.001). Further...

Physical Activity, Physical Performance, and Biological Markers of Health among Sedentary Older Latinos

Moreno, Gerardo; Mangione, Carol M.; Wang, Pin-Chieh; Trejo, Laura; Butch, Anthony; Tseng, Chi-Hong; Sarkisian, Catherine A.
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
EN
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46.03%
Background. Physical activity is associated with better physical health, possibly by changing biological markers of health such as waist circumference and inflammation, but these relationships are unclear and even less understood among older Latinos—a group with high rates of sedentary lifestyle. Methods. Participants were 120 sedentary older Latino adults from senior centers. Community-partnered research methods were used to recruit participants. Inflammatory (C-reactive protein) and metabolic markers of health (waist circumference, HDL-cholesterol, triglycerides, insulin, and glucose), physical activity (Yale physical activity survey), and physical performance (short physical performance NIA battery) were measured at baseline and 6-month followup. Results. Eighty percent of the sample was female. In final adjusted cross-sectional models, better physical activity indices were associated with faster gait speed (P < 0.05). In adjusted longitudinal analyses, change in self-reported physical activity level correlated inversely with change in CRP (β = −0.05; P = 0.03) and change in waist circumference (β = −0.16; P = 0.02). Biological markers of health did not mediate the relationship between physical activity and physical performance. Conclusion. In this community-partnered study...

Expressão de marcadores biológicos em câncer de mama antes e após a quimioterapia neoadjuvante. I- Correlações com desfechos clínicos e entre marcadores; Expression of biological markers in breast cancer before and after neoadjuvant chemotherapy. I – Correlations with clinical endpoints and between markers

Gabriel, Augusto Ribeiro
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Ciências da Saúde (FM); Faculdade de Medicina - FM (RG) Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa de Pós-graduação em Ciências da Saúde (FM); Faculdade de Medicina - FM (RG)
Tipo: Tese de Doutorado Formato: application/pdf
POR
Relevância na Pesquisa
46.09%
With the exception of skin cancer, breast cancer remains the most common malignant neoplasm affecting women both in Brazil and worldwide, inflicting severe economic, social and emotional consequences on patients and their families. Despite advances made in diagnostic and therapeutic techniques over recent decades, mortality rates from breast cancer remain expressive. To be able to treat tumors appropriately, not only profound knowledge of the cell mechanisms involved in their genesis, but also knowledge of the mechanisms involved in the success or failure of treatment is crucial. Various methods have been developed for this purpose, including the evaluation of biological tumor markers and the genetic studies. The objective of the present study was to evaluate some biological markers involved in the differentiation and evolution of breast cancer, using immunohistochemistry on tissue arrays. A retrospective study was conducted between 2006 and 2012 in which clinical data were obtained from patient charts, and tissue samples conserved in paraffin blocks were prospectively analyzed and correlated with each other and with the patient’s response to neoadjuvant chemotherapy. The results were presented in two papers. In the first article...

Socioeconomic status and health: exploring biological pathways

Lucas, Robyn
Fonte: Universidade Nacional da Austrália Publicador: Universidade Nacional da Austrália
Tipo: Thesis (PhD); Doctor of Philosophy (PhD)
EN
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55.97%
The cross-sectional Biomarkers Study was undertaken in Canberra, Australia (2000-2002) to examine the role of psychosocial factors in the socioeconomic health gradient, via physiological changes consequent upon activation of the neuroendocrine stress response.¶ The study population was derived from healthy 40-44 year old men and women already participating in a longitudinal cohort study. Using data from the cohort study, four groups with similar occupational status were formed. The study sample was randomly selected within these groups, thus representing the socioeconomic spectrum.¶ A pilot study involved 60 participants with blood and saliva samples measured on two occasions. A further 302 people had blood and saliva samples taken on one occasion. Socioeconomic status was measured by occupational code and status, personal and household income, education and perceived position in the community and in Australia. Psychosocial and behavioural factors, including job strain, job security, coping style, anxiety, depression, optimism, self-esteem, sense of belonging and trust, social support, smoking, exercise and alcohol intake were assessed by selfreport. Five biological parameters: plasma fibrinogen, glycated haemoglobin, waisthip ratio...

Seeking for biological markers in suicidal behaviour.; A procura de marcadores biológicos no comportamento suicidário.

Arantes-Gonçalves, Filipe; Serviço de Psiquiatria e Saúde Mental, Hospital de São João, Porto.; Coelho, Rui
Fonte: Ordem dos Médicos Publicador: Ordem dos Médicos
Tipo: info:eu-repo/semantics/article; article; article; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em 18/04/2008 POR
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Suicidal behaviour still represents a serious public health problem. Although the existence of some consultations on this subject, their efficacy remains very far from what we wish. In that sense, the research on the biological markers of suicidal behaviour might be of considerable importance. The aim of this paper is to present the biological systems involved on suicidal behaviour and to discuss if they can be used as a group of biological tests which could help clinical interview in predicting and preventing this kind of behaviour. It was done a Medline search between 1989 and 2007, considering the key-words Neurobiology and Suicide and therefore forty original or review articles were selected after reading each abstract content. From all the biological systems studied the one which shows more convincing data about its involvement in suicide is the serotoninergic system. At this level, we can say that there is a decreased neurotransmission of this monoamine in the Central Nervous System and Platelets as well as a compensatory increased binding of ligands to the serotoninergic receptors. At the same time, we have an hyperactivation of the HPA axis with lack of normalization of the Dexametasone Supression Test, decrease of neurotrophic genes like CREB...

ASSESSMENT OF THE RESPONSE OF PATIENTS WITH CROHN'S DISEASE TO BIOLOGICAL THERAPY USING NEW NON-INVASIVE MARKERS: lactoferrin and calprotectin

NOGUEIRA,Islaine Martins; MISZPUTEN,Sender Jankiel; AMBROGINI Jr.,Orlando; ARTIGIANI-NETO,Ricardo; CARVENTE,Cláudia Teresa; ZANON,Maria Ivani
Fonte: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED Publicador: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2013 EN
Relevância na Pesquisa
46.09%
Context The use of fecal markers to monitor Crohn's disease is crucial for assessing the response to treatment. Objective To assess the inflammatory activity of Crohn's disease by comparing fecal markers (calprotectin and lactoferrin), colonoscopy combined with biopsy, and the Crohn's disease activity index (CDAI), as well as serum markers, before treatment with infliximab, after the end of induction, and after the end of maintenance. Methods Seventeen patients were included who had been previously diagnosed with Crohn's disease and were using conventional treatment but required the introduction of biological therapy with infliximab. Each patient underwent a colonoscopy with biopsy, serum, and fecal (calprotectin and lactoferrin) tests to assess inflammatory activity, and CDAI assessments before treatment with infliximab, after induction (week 8), and after maintenance (week 32). Results The calprotectin levels exhibited significant reductions (P = 0.04) between the assessment before treatment with infliximab and the end of induction, which did not occur after the end of the maintenance phase. Lactoferrin remained positive throughout the three phases of the study. Regarding the histological assessment, a significant difference was found only between the assessment before treatment and after the end of maintenance (P = 0.036)...

Role of biological markers in the clinical outcome of colon cancer

Nanni, O; Volpi, A; Frassineti, G L; De Paola, F; Granato, A M; Dubini, A; Zoli, W; Scarpi, E; Turci, D; Oliverio, G; Gambi, A; Amadori, D
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
EN
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We investigated a number of biological markers, evaluated under strict intralaboratory quality control conditions, in terms of their role in predicting clinical outcome of patients with colon cancer treated with 5-FU-containing regimens. Colon cancer tissue from 263 patients enrolled onto two randomised clinical trials were studied for their cytofluorimetrically determined DNA content and their immunohistochemically evaluated microvessel density, vascular endothelial growth factor expression, thymidylate synthase expression and tumour lymphocyte infiltration. Disease-free survival and overall survival of patients were analysed as a function of the different variables. At a median follow up of 57 months, age, gender and Dukes' stage showed an impact on disease-free survival, whereas no biological marker emerged as an indicator of better or worse disease-free survival. Only histological grade and Dukes' stage were found to influence overall survival. The different biological variables, studied with particular attention for determination reliability, proved to have no impact on the clinical outcome of patients with colon cancer. Therefore, other markers must be identified to complement clinico-pathological variables in the management of this disease.

Biological markers of intermediate outcomes in studies of indoor air and other complex mixtures.

Wilcosky, T C
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /12/1993 EN
Relevância na Pesquisa
46.04%
Biological markers of intermediate health outcomes sometimes provide a superior alternative to traditional measures of pollutant-related disease. Some opportunities and methodologic issues associated with using markers are discussed in the context of exposures to four complex mixtures: environmental tobacco smoke and nitrogen dioxide, acid aerosols and oxidant outdoor pollution, environmental tobacco smoke and radon, and volatile organic compounds. For markers of intermediate health outcomes, the most important property is the positive predictive value for clinical outcomes of interest. Unless the marker has a known relationship with disease, a marker response conveys no information about disease risk. Most markers are nonspecific in that various exposures cause the same marker response. Although nonspecificity can be an asset in studies of complex mixtures, it leads to problems with confounding and dilution of exposure-response associations in the presence of other exposures. The timing of a marker's measurement in relation to the occurrence of exposure influences the ability to detect a response; measurements made too early or too late may underestimate the response's magnitude. Noninvasive markers, such as those measured in urine...