Página 1 dos resultados de 3911 itens digitais encontrados em 0.010 segundos

Signatures of selection in the Iberian honey bee: a genome wide approach using single nucleotide polymorphisms (SNPs)

Chavez-Galarza, Julio; Johnston, J. Spencer; Azevedo, João; Muñoz, Irene; De la Rúa, Pilar; Patton, John C.; Pinto, M. Alice
Fonte: Apimondia Publicador: Apimondia
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
37.19%
Dissecting genome-wide (expansions, contractions, admixture) from genome-specific effects (selection) is a goal of central importance in evolutionary biology because it leads to more robust inferences of demographic history and to identification of adaptive divergence. The publication of the honey bee genome and the development of high-density SNPs genotyping, provide us with powerful tools, allowing us to identify signatures of selection in the honey bee genome. These signatures will be an important first step towards understanding the transition of genotype into phenotype and the basis of adaptive divergence. The Iberian Peninsula harbours the greatest honey bee genetic diversity and complexity in Europe. The challenge of deciphering the mechanisms underlying such complexity has led to numerous morphological and molecular marker-based surveys of the Iberian honey bee. Yet, in spite of the numerous studies, the evolutionary processes underlying patterns of Iberian honey bee genetic diversity remain poorly understood. The evolutionary process in the Iberian Peninsula has been dynamic and the genetic consequences are too complex to be addressed piecemeal, using few markers with unknown or poorly known linkage relationships. Accordingly...

Signatures of selection in the Iberian honey bee: a genome wide approach using single nucleotide polymorphisms (SNPs)

Chavez-Galarza, Julio; Johnston, J. Spencer; Azevedo, João; Muñoz, Irene; De la Rúa, Pilar; Patton, John C.; Pinto, M. Alice
Fonte: Apimondia Publicador: Apimondia
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
37.19%
Dissecting genome-wide (expansions, contractions, admixture) from genome-specific effects (selection) is a goal of central importance in evolutionary biology because it leads to more robust inferences of demographic history and to identification of adaptive divergence. The publication of the honey bee genome and the development of high-density SNPs genotyping, provide us with powerful tools, allowing us to identify signatures of selection in the honey bee genome. These signatures will be an important first step towards understanding the transition of genotype into phenotype and the basis of adaptive divergence. The Iberian Peninsula harbours the greatest honey bee genetic diversity and complexity in Europe. The challenge of deciphering the mechanisms underlying such complexity has led to numerous morphological and molecular marker-based surveys of the Iberian honey bee. Yet, in spite of the numerous studies, the evolutionary processes underlying patterns of Iberian honey bee genetic diversity remain poorly understood. The evolutionary process in the Iberian Peninsula has been dynamic and the genetic consequences are too complex to be addressed piecemeal, using few markers with unknown or poorly known linkage relationships. Accordingly...

The impact of apiculture on the structure of Atlantic island honey bee populations

Muñoz, Irene; Pinto, M. Alice; De la Rúa, Pilar
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
37.08%
Beekeeping practices such as importation of non-native honeybee queens may interact with the conservation of honey bee subspecies and ecotypes biodiversity. Island honey bee populations are especially appropriate to test the impact of the introduction of foreign subspecies into their genetic diversity and structure. Here we used microsatellite markers to test whether genetic introgression from introduced honey bee queens is taken place in the honey bee populations from the Canary Islands, Azores and Madeira (Macaronesian region). We found introgression signals from foreign honeybee populations on Atlantic islands based on Bayesian structure analysis and Hardy-Weinberg equilibrium test. Fst pairwise comparisons with Iberian, North African and other European populations and structure analyses suggest different sources of honey bee queens into the islands: while honey bees from Canary Islands showed introgression from European honey bees, the honey bees from Azores and Madeira showed a closer relationship with those from the Iberian Peninsula. The existence of endemic honey bee populations on the Canary Islands prone to be conserved can still be depicted from the aforementioned analyses.

Introgression levels of the Italian and carniolan honey bee subspecies into the black honey bee: a comparison between microsatellite and single nucleotide polymorphism (SNP) markers

Henriques, Dora; Jara, Laura; Chavez-Galarza, Julio; Rufino, José; De la Rúa, Pilar; Pinto, M. Alice
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
37.11%
Human activities have been shaping the distribution of honey bee (Apis mellifera) subspecies in Europe. In fact, during the last decades there has been an extensive introduction of the beekeepers’ favorite eastern European (lineage C) subspecies A. m. ligustica (Italian honey bee) and A. m. carnica (carniolan honey bee) into western Europe. Resulting from these introductions, there has been gene flow, and in some regions even replacement, of the native western European subspecies A. m. mellifera (black honey bee), which belongs to lineage M. Assessing levels of introgression is an important activity in breeding programs, especially when conservation of native subspecies is a major concern. Previous surveys of the A. m. mellifera populations estimated the introgression of lineage C into lineage M honey bees by using mtDNA and microsatellite markers. In addition to these markers others, such as SNPs, can be used. SNPs have some advantages over other molecular markers as they provide a genome wide coverage, higher quality data, and at the same time they are suitable for automatic and standardization in high throughput technologies. Previous studies indicate that the discriminatory power of SNPs to detect population structure is lower than that of microsatellites; about 100 SNPs are needed to provide the same power of 10-20 microsatellites. In this study we will compare introgression levels between microsatellites and SNPs as estimated by the software STRUCTURE using the admixture model and correlated allele frequencies in a black honey bee collection originating from several countries across western Europe. This collection was genotyped for 12 microsatellites and 1183 SNPs. The introgression levels were first estimated using both full datasets. Then...

Introgression levels of the Italian and carniolan honey bee subspecies into the black honey bee: a comparison between microsatellite and single nucleotide polymorphism (SNP) markers

Henriques, Dora; Jara, Laura; Chavez-Galarza, Julio; Rufino, José; De la Rúa, Pilar; Pinto, M. Alice
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
37.11%
Human activities have been shaping the distribution of honey bee (Apis mellifera) subspecies in Europe. In fact, during the last decades there has been an extensive introduction of the beekeepers’ favorite eastern European (lineage C) subspecies A. m. ligustica (Italian honey bee) and A. m. carnica (carniolan honey bee) into western Europe. Resulting from these introductions, there has been gene flow, and in some regions even replacement, of the native western European subspecies A. m. mellifera (black honey bee), which belongs to lineage M. Assessing levels of introgression is an important activity in breeding programs, especially when conservation of native subspecies is a major concern. Previous surveys of the A. m. mellifera populations estimated the introgression of lineage C into lineage M honey bees by using mtDNA and microsatellite markers. In addition to these markers others, such as SNPs, can be used. SNPs have some advantages over other molecular markers as they provide a genome wide coverage, higher quality data, and at the same time they are suitable for automatic and standardization in high throughput technologies. Previous studies indicate that the discriminatory power of SNPs to detect population structure is lower than that of microsatellites; about 100 SNPs are needed to provide the same power of 10-20 microsatellites. In this study we will compare introgression levels between microsatellites and SNPs as estimated by the software STRUCTURE using the admixture model and correlated allele frequencies in a black honey bee collection originating from several countries across western Europe. This collection was genotyped for 12 microsatellites and 1183 SNPs. The introgression levels were first estimated using both full datasets. Then...

Brazilian samples of bee samples pollen: palynological origin, phenolic content, antioxidant properties and antimicrobial activity

Arruda, Vanilda Aparecida Soares de; Santos, Alexandre Vieira dos; Figueiredo, Davi; Meira, Sampaio; Estevinho, Leticia M.; Barth, Ortrud Monika; Freitas, Alex da Silva de; Almeida-Muradian, Ligia Bicudo
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
37.08%
Total phenolic and flavonoids phytochemical concentration was measured in bee pollen samples, Appis Mellifera , as well as their botanical origin, antioxidant activity and antimicrobial activity. Pollen loads were washed with 70% ethanol and identified using a 400x magnification. The content of total polyphenols and flavonoids was measured spectrophotometrically with gallic acid and quercetin as standard. The antioxidant activity was evaluated by the linking capacity of (i)the free radical DPPH (2,2-Diphenyl-1-picrylhydrazyl), (ii)ORAC and (iii)system β-carotene/linoleic acid. The experiments were performed in triplicate and the results expressed as mean ± standard deviation. All statistical analysis were performed using the program STATISTICA 8.0 and adopting the significance level of 5% (p<0.05). Were using six samples of dehydrated bee pollen collected in Ribeirao Preto, SP, Brazil during the years of 2010 and 2011. Five families were found in the mixture of bee pollen: Mimosa caesalpiniaefolia , Mimosa scabrella, Asteraceae, Poaceae, Zea mays. Each dried bee pollen sample was composed mainly of Mimosa caesalpiniaefolia (Fabaceae-Mimosoideae). The variations were (dry basis): 17.64±1.57 to 32.14±2,88 mg GAE/g of bee pollen for phenolic compounds; 2.51±0.28 to 3.71±0.25 mg quercetin/g of pollen for flavonoids concentration; 77.15±0.72 to 90.56% for antioxidant activity (DPPH) and 3.19±0.25 to 4.61±0.33 mg/mL for EC50; 183.42±16.26 to 338.32± 33.38 μmols eq. Trolox/g for ORAC and 68.96±2.75 to 85.90±2.27% for β -carotene/linoleic acid. Samples of pollen...

Palynological and physicochemical characterization of Apis mellifera L. bee pollen in the Southern region of Brazil

CARPES, Solange T.; CABRAL, Ingridy S. R.; LUZ, Cynthia Fernandez P.; CAPELETTI, Jailson P.; ALENCAR, Severino Matias; MASSON, Maria Lucia
Fonte: WFL PUBL Publicador: WFL PUBL
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
37.08%
Bee pollen has been used for many years in both traditional medicine and supplementary nutrition, as well as in alternative diets, mainly due to its nutritional properties and health benefits. Bee pollen production is a recent activity in Brazil, having begun in the late 1980s. However, the country has the potential of being a large world producer of high quality pollen, particularly because of the great diversity of tropical flora and the resistance of the Brazilian Apis mellifera bee races. Thirty-six samples of bee pollen from the Southern region of Brazil were analyzed regarding pollen types and physicochemical and nutritional composition. Only one sample was considered monofloral, which was exclusively composed by pollen from the Asteraceae family). The State of Parana showed a greater variety of pollen types, 18 in total, representing 82% of the total number identified in this study. The bee pollen in the States of Rio Grande do Sul and Parana showed a higher number of samples with humidity content above the standard permitted by the Brazilian legislation, i.e. over 4%. The bee pollen was characterized by its high protein content with average values of 20.47%. The analysis regarding humidity, lipids and sugar showed no statistical differences among the samples (p<0.05). The pollen samples had a high concentration of reducible sugars (48%). The predominant minerals in the samples PR...

Pollen substitutes increase honey bee haemolymph protein levels as much as or more than does pollen

JONG, David De; SILVA, Eduardo Junqueira da; KEVAN, Peter G.; ATKINSON, James L.
Fonte: INT BEE RESEARCH ASSOC Publicador: INT BEE RESEARCH ASSOC
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
47.16%
Adequate substitutes for pollen are necessary for maintaining healthy bee colonies during periods of pollen dearth, but testing them objectively is both time consuming and expensive. We compared two commercial diets with bee collected pollen and acacia pod flour (used by beekeepers in some parts of Brazil) by measuring their effect on haemolymph protein contents of young bees exclusively fed on these diets, which is a fast and inexpensive assay. The commercial diets included a new, non-soy-based, pollen substitute diet (named Feed-Bee (R)) and a soy-based diet, named Bee-Pro (R). The diets were each given in patty form to groups of 100 Africanized honey bees in hoarding cages, maintained and fed from emergence until six days of age. Sucrose, in the form of sugar syrup, was used as a protein free control. Feed-Bee (R), Bee-Pro (R), pollen and acacia pod flour diets increased protein titers in the haemolymph by factors of 2.65, 2.51, 1.76 and 1.69, respectively, over protein titers in bees fed only sucrose solution. The bees fed Feed-Bee (R) and Bee-Pro (R) had their haemolymph significantly enriched in protein compared to the controls and those fed acacia pod flour and to titers slightly higher than those fed pollen. All four proteinaceous diets were significantly superior to sucrose alone.; CNPq; FAPESP

Sistema automatizado de classificação de abelhas baseado em reconhecimento de padrões.; Automated bee classification system based on pattern recognition.

Bueno, Jésus Franco
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 08/10/2010 PT
Relevância na Pesquisa
37.21%
A crescente perda mundial de biodiversidade tem sido uma das preocupações da comunidade científica internacional que motivaram a criação em 1992 da Convenção sobre a Diversidade Biológica, tornando-se um tratado de compromisso aceito pelos governantes de 187 países e pela Comunidade Européia. A redução da biodiversidade, devido a vários fatores, como a ação antrópica e o aquecimento global, compromete a capacidade do planeta de sustentação da vida humana em face do esgotamento dos recursos e serviços por ela prestados. A conservação e uso sustentável da biodiversidade passa necessariamente pela aquilatação e conhecimento das espécies. Entre essas espécies as abelhas polinizadoras têm merecido especial atenção, pois a polinização das plantas é um serviço de ecossistema muito importante. Cerca de três quartos das mais de 240 mil espécies de plantas do mundo dependem de polinizadores e estima-se que as abelhas sejam responsáveis por mais de 70% do serviço global de polinização. Existem quase 20.000 espécies de abelhas descritas no mundo. No Brasil são conhecidas quase 400 espécies de abelhas (cerca de 300 são abelhas sem ferrão) e o número estimado é de mais de 3.000 espécies. No entanto...

Pólen apícola desidratado: composição físico-química, qualidade microbiologica, compostos fenólicos e flavonoides, capacidade antioxidante e origem botânica; Dehydrtated bee pollen: physicochemical, microbiological quality, phenolic and flavonoids compounds, antioxidant and botanical origin.

Arruda, Vanilda Aparecida Soares de
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 10/10/2013 PT
Relevância na Pesquisa
37.18%
O pólen apícola, produto da aglutinação do pólen das flores com néctar e substâncias salivares das abelhas, tem sido promovido como suplemento da dieta humana por apresentar propriedades nutricionais e bioativas. Sessenta e duas amostras de pólen apícola desidratado foram avaliadas para as análises de compostos fenólicos, flavonoides, atividade antioxidante por três métodos (DPPH, sistema β-caroteno e ORAC), origem botânica, qualidade comercial (umidade, cinzas, lipídeos, proteínas e carboidratos) e sanitária (bolores e leveduras, aeróbios mesófilos, coliformes totais, E.coli, clostrídios sulfito redutores e S.aureus.), além da atividade antimicrobiana (Candida albicans, Escherichia coli, Staphylococcus aureus). Os valores obtidos para compostos fenólicos e flavonoides totais variaram de 12,60 a 84,22 mg GAE/g de pólen apícola (GAE: equivalentes em ácido gálico) e 1,90 a 36,85 mg de quercetina/g de pólen apícola respectivamente. O EC50, determinado pelo método do DPPH, variou de 0,35 a 13,42 mg pólen apícola/mL de extrato. Os extratos de pólen apícola apresentaram valores entre 52,58 e 98,37 % para o método do β-caroteno. Quando quantificada por ORAC, a atividade antioxidante medida ficou entre 132...

Nutritive value and apparent digestibility of bee-collected and bee-stored pollen in the stingless bee, Scaptotrigona postica Latr. (Hymenoptera, Apidae, Meliponini)

Fernandes-da-Silva, P. G.; Serrao, J. E.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 39-45
ENG
Relevância na Pesquisa
37.08%
In Meliponini bees, pollen is stored inside the hive. We compared the nutritional value and apparent digestibility of bee-collected and bee-stored pollen of Scaptotrigona postica. The results showed no differences in nutritional value and apparent digestibility between bee-collected and bee-stored pollen, suggesting that the storage process of pollen grains do not play a role in these parameters. These results are discussed in relation to other aspects of pollen storage, such as food conservation and/or organoleptic properties of stored pollen.

Nutritive value and apparent digestibility of bee-collected and bee- stored pollen in the stingless bee, Scaptotrigona postica Latr. (Hymenoptera, Apidae, Meliponini)

Fernandes-Da-Silva, Pedro Guilherme; Serrão, José Eduardo
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 39-45
ENG
Relevância na Pesquisa
37.11%
In Meliponini bees, pollen is stored inside the hive. We compared the nutritional value and apparent digestibility of bee-collected and bee-stored pollen of Scaptotrigona postica. The results showed no differences in nutritional value and apparent digestibility between bee-collected and bee- stored pollen, suggesting that the storage process of pollen grains do not play a role in these parameters. These results are discussed in relation to other aspects of pollen storage, such as food conservation and/or organoleptic properties of stored pollen.

Use of bee-attractants in increasing crop productivity in Niger (Guizotia abyssinica. L)

Sivaram,Venkataramegowda; Jayaramappa,Koragandahalli Venkateshappa; Menon,Anita; Ceballos,Ruben Michael
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2013 EN
Relevância na Pesquisa
37.16%
The use of bee attractants, Bee-Q and Fruit BoostTM in the pollination of niger was evaluated. Bee visitations to niger flowers were observed for two weeks and an estimation of seed yield was determined. Different concentrations of Bee-Q and Fruit boostTM were evaluated to understand honeybee visitation patterns on the target crop and pollination efficiency. Results indicate that applications of Bee-Q at 12.5 g/l and Fruit boost at 0.75 ml/l on niger plots significantly increased the number of bee foragers over control plots. In addition, plots sprayed with these bee attractants significantly enhanced the seed set, seed weight, and germination of niger. This study suggests that pheromone-based bee attractants applied to niger can increase the marginal percentage of bee visitation, seed yield, and percent germination.

Proteomic analyses of male contributions to honey bee sperm storage and mating

Collins, A M; Caperna, T J; Williams, V; Garrett, W M; Evans, J D
Fonte: Blackwell Publishing Ltd Publicador: Blackwell Publishing Ltd
Tipo: Artigo de Revista Científica
Publicado em 01/10/2006 EN
Relevância na Pesquisa
37.08%
Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against three databases, general species, honey bees and fruit flies, were performed. Spectra were used to query the recently generated honey bee genome protein list as well as general species and fruit fly databases. Of the 69 unique honey bee proteins found, 66 are also in Drosophila melanogaster. Two proteins only matched honey bee genes and one is a widespread protein lost from the fly genome. There is over-representation of genes implicated in the glycolysis pathway. Metabolism-associated proteins were found primarily in the seminal vesicle. Male accessory gland proteins as identified in Drosophila rarely had orthologs among proteins found in the honey bee. A complete listing of gel spots chosen including honey bee genome matches and Mascot searches of MALDI-TOF results with statistics is in the Supplementary table. MALDI-TOF spectra and more complete Mascot peptide mass fingerprinting data are available on request. Supplementary figs 1–3 show the stained protein gels.

Pólen apícola do estado de Alagoas : composição físico-química, origem botânica e atividade antioxidante.; Bee pollen of Alagoas State : physico-chemical composition, botanical origin and antioxidant activity.

Vasconcelos, Maria Raphaella dos Santos
Fonte: Universidade Federal de Alagoas; BR; Nutrição; Programa de Pós-Graduação em Nutrição; UFAL Publicador: Universidade Federal de Alagoas; BR; Nutrição; Programa de Pós-Graduação em Nutrição; UFAL
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
37.19%
Beekeeping is an activity known worldwide. In Brazil, it has found favorable conditions for its establishment. In the State of Alagoas, where it is still a practicing of beekeeping craft, require official data on the quality of their products to become more professional. Among the bee products, bee pollen resulting from the agglutination of flower pollen and nectar with salivary substances, emerge as food for worker-bees, with therapeutic properties given its composition. It is rich in protein, carbohydrates, lipids, minerals and vitamins, phenols and growth factors. In apiaries, bee pollen is the main source of food and protection for the larvae of bees. As human food, it requires for marketing monitoring of its physico-chemical and microbiological quality, with limits established by the health legislation. In this work, it was assessed the floral origin and the physico-chemical, and possible antioxidant and antimicrobial activity, of bee pollen from three experimental apiaries representative of the mesoregions of Zona da Mata , Backlands and Seaside of Alagoas State, in the dry season of 2008/09. Physico-chemical data were submitted to tests of normality of Kolmogorov-Smirnov and Shapiro-Wilk, and then the non-parametric test of Kruskal-Wallis. The pollen spectrum was more abundant in the Zona da Mata region...

Bee Communities in Restored Landfill Sites of Niagara Region

Kutby, Rola
Fonte: Brock University Publicador: Brock University
Tipo: Electronic Thesis or Dissertation
ENG
Relevância na Pesquisa
37.24%
This study examined the impact of habitat restoration on bee communities (Hymenoptera: Apidae) of the Niagara Region, Ontario, Canada. Bee abundance and diversity was studied in three restored landfill sites: the Glenridge Quarry Naturalization Site (GQNS) in St. Catharines, Elm Street Naturalization Site in Port Colborne, and Station Road Naturalization Site in Wainfleet during 2011 and 2012. GQNS represented older sites restored from 2001-2003. Elm and Station sites represented newly restored landfills as of 2011. These sites were compared to control sites at Brock University where bee communities are well established and again to other landfills where no stable habitat was available before restoration. The objective of this study is to investigate the impact of restoration level on bee abundance and diversity in restored landfill sites of the Niagara Region. Based on the increased disturbance hypothesis (InDH) and the intermediate disturbance hypothesis (IDH), I hypothesized that bee abundance and diversity will follow two patterns. First pattern according to InDH suggest that as the disturbance decrease the bee abundance and diversity will increased. Second pattern according to the IDH bee abundance and diversity will be the highest at the intermediate level of disturbance. A total of 7 173 bees were collected using pan traps and flower collections...

Hochregulierung des Aktivierungsmarkers E-NPP3 (CD203c) auf der Oberfläche basophiler Granulozyten nach In-vitro-Stimulation mit definierten Bienen- und Wespengiftallergenen bei Insektengiftallergikern; Individual bee and wasp venom compounds induce upregulation of the basophil activation marker E-NPP3 (CD203c) in patients with hymenoptera venom allergy

Binder, Marc
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
DE_DE
Relevância na Pesquisa
37.11%
Einleitung: Der Einsatz definierter Einzelallergene anstelle von Allergenextrakten hat in der Diagnostik von IgE-Antikörper-vermittelten Soforttypallergien zunehmend an Bedeutung gewonnen. Die Gifte der Biene (Apis mellifera) und der Wespe (Vespula vulgaris) enthalten zahlreiche verschiedene Einzelkomponenten. Eine allergene Wirkung ist vor allem für die hochmolekularen Proteine der Insektengifte bekannt. Um die Sensibilisierung gegenüber definierten Bienen- und Wespengiftallergenen zu untersuchen, wurde in dieser Studie ein kürzlich beschriebener In-vitro-Allergietest verwendet und weiterentwickelt. Er basiert auf der Allergen-induzierten Hochregulierung der CD203c-Expression auf basophilen Granulozyten. Patienten und Methoden: Vollblutproben von 41 Patienten mit Verdacht auf Bienen- und/ oder Wespengiftallergie sowie von 9 Kontrollpersonen wurden mit den gereinigten Bienengiftallergenen Phospholipase A2 (Api m1), Hyaluronidase (Api m2) und Melittin (Api m4) oder den gereinigten Wespengiftallergenen Phospholipase A1 (Ves v1), Hyaluronidase (Ves v2) und rekombinantem Antigen 5 (Ves v5) stimuliert. Zusätzlich wurden zwei gereinigte Allergene der Papierwespe sowie herkömmliches Bienen- und Wespengiftextrakt eingesetzt. PBS diente als Negativ-...

Quantificação das vitaminas do complexo B (B1, B2) e vitâmeros das vitaminas B3 e B6 em amostras de pólen apícola desidratado provenientes da Região Sul do Brasil; Quantification of B complex vitamins (B1, B2) and vitamers of vitamins B3 and B6 in dehydrated bee pollen samples from Southern Brazil

Souza, Bianca Rodrigues de
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 22/09/2014 PT
Relevância na Pesquisa
37.08%
Entende-se por pólen apícola o resultado da aglutinação do pólen das flores, efetuado pelas abelhas operárias, mediante néctar e substâncias salivares, o qual é recolhido no ingresso da colmeia. A literatura descreve que esse alimento contém proteínas, carboidratos, lipídeos, vitaminas e minerais. De acordo com estudo prévio, amostras de pólen apícola in natura e desidratado, da cidade de Pariquera-Açu (São Paulo), apresentaram teores significativos de vitamina B1(tiamina) e B2 (riboflavina), além da presença dos vitâmeros da vitamina B3 (ácido nicotínico e nicotinamida) e B6 (piridoxal, piridoxol e piridoxamina) em sua composição o que foi associado à flora local explorada pelas abelhas. A região Sul do Brasil possui clima, relevo e vegetação diferenciados de outras regiões, necessitando-se assim da verificação do potencial vitamínico deste produto local. Destaca-se, ainda, o fato de que nesta região encontra-se um dos dois maiores produtores nacionais de pólen apícola (estado de Santa Catarina). O presente trabalho teve como objetivo principal quantificar os teores das vitaminas do complexo B: vitaminas B1 e B2, assim como os vitâmeros das vitaminas B3 e B6. Foram coletados 28 lotes de pólen apícola desidratado de diferentes localidades da região Sul durante o período de agosto de 2011 a dezembro de 2012 que posteriormente foram armazenados...

A review of methods for discrimination of honey bee populations as applied to European beekeeping

Bouga, Maria; Alaux, Cedric; Bienkowska, Malgorzata; Büchler, Ralph; Carreck, Norman; Cauia, Eliza; Chlebo, Robert; Dahle, Bjorn; Dall'Olio, Raffaele; De la Rúa, Pilar; Gregorc, Ales; Ivanova, Evgeniya; Kence, Aykut; Kence, Meral; Kezic, Nikola; Kiprija
Fonte: International Bee Research Association Publicador: International Bee Research Association
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
46.93%
Here, scientists from 19 European countries, most of them collaborating in Working Group 4: “Diversity and Vitality” of COST Action FA 0803 “Prevention of honey bee COlony LOSSes” (COLOSS), review the methodology applied in each country for discriminating between honey bee populations. Morphometric analyses (classical and geometric) and different molecular markers have been applied. Even if the approach has been similar, however, different methodologies regarding measurements, landmarks or molecular markers may have been used, as well as different statistical procedures. There is therefore the necessity to establish common methods in all countries in order to have results that can be directly compared. This is one of the goals of WG4 of the COLOSS project.

Nutritive value and apparent digestibility of bee-collected and bee- stored pollen in the stingless bee, Scaptotrigona postica Latr. (Hymenoptera, Apidae, Meliponini)

Fernandes-da-Silva, Pedro Guilherme; Serrão, José Eduardo
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 39-45
ENG
Relevância na Pesquisa
37.11%
In Meliponini bees, pollen is stored inside the hive. We compared the nutritional value and apparent digestibility of bee-collected and bee-stored pollen of Scaptotrigona postica. The results showed no differences in nutritional value and apparent digestibility between bee-collected and bee- stored pollen, suggesting that the storage process of pollen grains do not play a role in these parameters. These results are discussed in relation to other aspects of pollen storage, such as food conservation and/or organoleptic properties of stored pollen.