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Flora bacteriana e citoquínas pró-inflamatórias no trato digestório exclusivo após cirurgia de derivação em Y de Roux para obesidade mórbida; Microbial flora and proinflammatory cytokines in excluded digestive tract after Roux en-Y gastric bypass for morbid obesity

Ishida, Robson Kiyoshi
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 10/10/2007 PT
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Introdução: Em estudo prospectivo, os efeitos da gastroplastia redutora com reconstrução em Y de Roux sobre a flora bacteriana e produção de citoquinas nas câmaras gástricas proximal e excluída foram estudados. Métodos: pacientes bariátricos (n=37) foram submetidos à avaliação endoscópica em ambos reservatórios gástricos,7,3+-1,4 anos após a gastroplastia. Idade foi de 42,4+-9,9 anos (70,2% sexo feminino), IMC pré-operatório de 53,5+-10,6, e IMC atual de 32,6+-7,8kg/m2. TNFalfa e TGF-beta foram medidos pelo método ELISA em biópsias da mucosa gástrica., assim como cultura quantitativa da secreção gástrica, com pH gástrico e teste respiratório lactulose/hidrogênio.Resultados: Nenhum dos pacientes apresentou queixas sugestivas de supercrescimento bacteriano gastrointestinal. Todavia, contagens elevadas de bactérias e fungos foram identificadas nas duas câmaras, principalmente no estômago proximal. Gram-positivos representaram a maioria dos isolados. O pH foi neutro na câmara proximal, enquanto que também na câmara distal nem sempre conservou-se em níveis esperados. Conclusões: 1)Produção elevadas de TNF-alfa e TGF-beta, com a colonização de aeróbios, anaeróbios e fungos em ambas câmaras gástricas foram identificadas; 2)O pH gástrico como a contagem bacteriana foram maiores no estômago proximal funcionante; 3)Teste respiratório foi positivo para supercrescimento bacteriano em 40...

Caracterização da microbiota mesófila aeróbia isolada de um sistema de lagoas de estabilização para o tratamento de dejetos de suínos; Characterization of the aerobic mesophilic bacterial flora isolated from a stabilization ponds system processing pig slurry

Schmidt, Veronica; Santin, Katiane; Cardoso, Marisa Ribeiro de Itapema
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
POR
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O comportamento de populações microbianas mesófilas, presentes em dejetos suínos submetidos a tratamento em lagoas de estabilização, ainda é pouco conhecido. O objetivo deste estudo foi avaliar a variação quantitativa e qualitativa da microbiota mesófila aeróbia presente ao longo de um sistema de lagoas de estabilização para tratamento de dejetos suínos. A população média de mesófilos aeróbios manteve-se constante ao longo das lagoas, apesar de ter havido uma variação nas contagens entre as diferentes coletas realizadas. A diversidade média da microbiota do efluente foi menor que a encontrada no afluente do sistema, não havendo diferença na equitabilidade entre os pontos amostrados. No início do sistema houve um predomínio de bactérias com características compatíveis com as enterobactérias, enquanto no efluente predominaram grupos semelhantes aos enterococos.; The mesophilic bacteria species pattern, found in pig slurry submited to treatment in stabilization ponds, is still poorly understood. The aim of this study was to evaluate the quantitative and qualitative variation of the aerobic mesophilic flora isolated throughout a stabilization ponds system, that receives pig slurry. The mesophilic population counts didn’t show any variantion among the sampled ponds but different counts have been found among the sampling periods. The bacterial flora of the effluent showed a lower diversity than that found in the affluent. There was no difference in the equitability among the sampled points of the system. Most of the affluent isolates showed morphological and biochemical characteristics similar to the Enterobacteriaceae family...

Effects of an Antibacterial Soap on the Ecology of Aerobic Bacterial Flora of Human Skin

Voss, J. G.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /10/1975 EN
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The effects of ad lib use of an antibacterial soap containing 1.0% trichlorocarbanilide and 0.5% trifluoromethyldichlorocarbanilide on the bacterial flora of six skin sites of 132 subjects were measured by comparison with the flora of 93 control subjects who avoided the use of topical antibacterials. Each subject was examined once. The test soap produced significant reductions in geometric mean counts of the total aerobic flora on the back, chest, forearm, calf, and foot; counts were also reduced in the axilla, but not to a significant extent. The overall reduction by the test soap on all sites was 62% (P < 0.001). Neither age nor sex influenced the effect of the soap on the flora. The antibacterial soap also reduced the prevalence of Staphylococcus aureus on the skin, mostly by virtually eliminating it from areas other than the axilla. Partial inhibition of the gram-positive flora was not accompanied by an increase in gram-negative species. The latter were found principally in the axilla; Klebsiella pneumoniae and Enterobacter aerogenes were the species most frequently found.

Cecal and fecal bacterial flora of the Mongolian gerbil and the chinchilla.

Worthington, J M; Fulghum, R S
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1988 EN
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The Mongolian gerbil is being increasingly used as a laboratory animal and as a pet. Both chinchillas and gerbils are used as animal models for otitis media and other otic research. Previously, only incomplete information was available regarding the indigenous bacterial flora of the lower intestinal tracts of these coprophagic animals. Using the strict anaerobic methodology of the Virginia Polytechnic Institute Anaerobe Laboratory, we studied the predominant bacterial flora of the cecum and fecal pellets of the gerbil and the chinchilla and the bacterial flora of digesta pellets in the proximal colon. We found species of the following anaerobic genera in high dilutions of gerbil fecal pellets: Bifidobacterium, Clostridium, Propionibacterium, Lactobacillus, and Bacteroides. Only lactobacilli were found in high dilutions of digesta from the upper colon, although the cecum yielded Peptostreptococcus, Bifidobacterium, Clostridium, Lactobacillus, Propionibacterium, and Bacteroides species from high dilutions of cecal contents. The facultatively anaerobic and aerobic flora isolated consisted of species of Bacillus, Streptococcus, Staphylococcus, Acinetobacter, Alcaligenes, Escherichia, Pasteurella, and Pseudomonas plus several unidentifiable organisms. Species of Bifidobacterium...

Bacterial flora in spontaneously occurring aural cholesteatomas in Mongolian gerbils.

Fulghum, R S; Chole, R A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /12/1985 EN
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Bacteria were isolated from 29 Mongolian gerbils, Meriones unguiculatus, with spontaneous aural cholesteatomas. We identified 148 cultures, 44 from the middle ear surface of cholesteatomas and 104 from cholesteatoma contents. We could only identify 63 cultures to the genus level, although we identified 85 cultures as belonging to 21 different species. We found on the surfaces of cholesteatomas representatives of 9 genera, from which 8 species could be identified, and representatives of 19 genera within the cholesteatoma sac, from which 21 species could be identified. The most common bacterial genera isolated were Staphylococcus, Pseudomonas, and Corynebacterium among the aerobic genera, and Bacteroides and Peptococcus among the anaerobic genera. The bacterial flora of gerbilline cholesteatomas was found to be diverse, resembling the flora found to be associated with human cholesteatomas. The flora also resembled the organisms found transitorily within the normal middle ear cavity of gerbils, except for a higher incidence of pseudomonads.

Intestinal Bacterial Flora and Transit Time of Three Neotropical Bat Species

Klite, P. D.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /08/1965 EN
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Klite, P. D. (Middle America Research Unit, Balboa Heights, Canal Zone). Intestinal bacterial flora and transit time of three neotropical bat species. J. Bacteriol. 90:375–379. 1965.—Quantitative studies on the intestinal bacterial flora of three neotropical bat species revealed the following average bacterial populations: Molossus major, 104.8 bacteria per intestinal contents; Carollia perspicillata, 103.3; Chilonycteris rubiginosa, 103.9. In comparison, laboratory mice had an average of 109.7 bacteria per intestinal contents. Of 236 bacterial isolates obtained from 60 bats, bacteria of the Klebsiella-Aerobacter-Serratia group were found most frequently, followed by enterococci and Proteus spp. Bacteria of eight other groups were less frequently recovered. A large intestine, cecum, or appendix was absent in all three bat species, and the intestinal length was one-third to one-fifth of that in a mouse of comparable weight. The transit time through the short bat intestine was 15 min. The possible relationship of these unusual anatomical and physiological phenomena to the ability of Histoplasma capsulatum to survive in bat feces is discussed.

An Abundance of Escherichia coli Is Harbored by the Mucosa- Associated Bacterial Flora of Interleukin-2-Deficient Mice

Schuppler, M.; Lötzsch, K.; Waidmann, M.; Autenrieth, I. B.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /04/2004 EN
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Mice deficient in interleukin-2 are well suited for use as an animal model for inflammatory bowel disease. Raised under specific-pathogen-free conditions, interleukin-2-deficient mice develop an inflammatory bowel disease resembling ulcerative colitis in humans. The finding that colitis was attenuated when the mice were kept under germfree conditions implies that the resident intestinal flora is involved in the pathogenesis of colitis. The present study addresses the composition of the mucosa-associated bacterial flora in colon samples from interleukin-2-deficient mice that developed colitis. This was investigated by comparative 16S ribosomal DNA (rDNA) sequence analysis and fluorescence in situ hybridization using rRNA-targeted fluorescent probes to quantify the bacterial populations of the mucosa-associated flora. The investigations revealed distinct differences in the bacterial composition of the mucosa-associated flora between interleukin-2-deficient mice and healthy controls. Fluorescence in situ hybridization identified up to 10% of the mucosa-associated flora in interleukin-2-deficient mice as Escherichia coli, whereas no E. coli was detected in the mucosa from healthy wild-type mice. This finding was consistent with the results from comparative 16S rDNA analysis. About one-third of the clones analyzed from 16S rDNA libraries of interleukin-2-deficient mice represented Enterobacteriaceae...

Susceptibility to adjuvant arthritis: relative importance of adrenal activity and bacterial flora.

van de Langerijt, A G; van Lent, P L; Hermus, A R; Sweep, C G; Cools, A R; van den Berg, W B
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /07/1994 EN
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Previous studies on the regulation of bacterial-induced arthritis in rats have focused on endocrine aspects as well as differences in T cell immunity against bacterial epitopes. We analysed the role of both adrenal activity and bacterial flora in determining susceptibility to bacterial-induced arthritis. Outbred Wistar rats show a low incidence of adjuvant arthritis. Moderate sensitivity to adjuvant arthritis was found in a selected, stress-resistant line of the Wistar rat, whereas no arthritis was found in a stress-susceptible Wistar line. Plasma corticosterone responses after IL-1 alpha exposure were, however, identical in these two lines, excluding a direct correlation between susceptibility and corticosterone levels. In line with previous findings in germ-free (GF) F344 rats, GF Wistars also appeared highly susceptible to arthritis. We further analysed the corticosterone responses in GF and conventional (CV) rats. Administration of IL-1 alpha induced identical corticosterone responses in both CV and GF F344 rats. In addition, plasma corticosterone levels were measured around the time of onset of arthritis. Whereas no rise was seen in the arthritis-resistant CV rats, a significant increase was observed from day 14 in GF rats, at the moment of onset of arthritis. Although this corticosterone response was insufficient to prevent arthritis...

Alterations in the conjunctival bacterial flora following a single dose of azithromycin in a trachoma endemic area

Chern, K.; Shrestha, S; Cevallos, V.; Dhami, H; Tiwari, P.; Chern, L.; Whitcher, J.; Lietman, T.
Fonte: BMJ Group Publicador: BMJ Group
Tipo: Artigo de Revista Científica
Publicado em /12/1999 EN
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BACKGROUND/AIMS—The World Health Organisation has recommended repeated mass treatment of children in trachoma endemic areas with oral azithromycin. While chlamydia, the causative agent of trachoma, remains universally sensitive to azithromycin, there is concern that large scale programmes may alter the bacterial flora and induce resistance in streptococcal species. In this study the effect of a single dose of azithromcyin on the prevalence, species distribution, and resistance of conjunctival bacterial flora was determined.
METHODS—Baseline and 14 day follow up bacterial cultures were taken from the conjunctivae of 121 children who reside in a trachoma endemic area of Nepal. 91 children were treated with azithromycin at baseline and 31 children received deferred treatment at the 14 day follow up.
RESULTS—Although the prevalence of bacterial pathogens decreased significantly with azithromycin treatment, a significant change in the distribution of specific bacterial pathogens could not be demonstrated. Streptococcal resistance to azithromycin was found significantly more frequently after treatment. No change in the prevalence, distribution, or resistance pattern was found in the untreated control group.
CONCLUSION—Repeated mass treatment of trachoma endemic areas with oral azithromycin will have an effect on bacterial flora. However...

Pulmonary Infection Due to Disruption of the Pharyngeal Bacterial Flora by Antibiotics in Hamsters

Dalton, Harry P.; Muhovich, Michaeleen; Escobar, Mario R.; Allison, Marvin J.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1974 EN
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An animal model was used to determine the effect of oxacillin on the pharyngeal bacterial flora and the relationship of this flora to pneumonia. The pharyngeal bacterial flora of 68 healthy Golden Syrian hamsters was determined. A quantitative comparison between Streptococci and Escherichia, Proteus, Klebsiella and Enterobacter from 70 hamsters was made before and at 4, 24, 48 and 72 hours after oxacillin administration. Lung cultures were positive in 22 of 25 hamsters, yielding K pneumoniae type 1 most frequently. Lung histology from 25 hamsters revealed bronchopneumonia. Intestinal postmortem cultures of treated and untreated animals were similar. The importance of throat cultures in diagnosing pneumonia and the value of the hamster model to study the effect of other antibiotics on the temporary flora are demonstrated.

The development of bacterial flora of premature neonates.

Rotimi, V. O.; Olowe, S. A.; Ahmed, I.
Fonte: Cambridge University Press Publicador: Cambridge University Press
Tipo: Artigo de Revista Científica
Publicado em /06/1985 EN
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The sequential acquisition of bacterial flora by premature neonates was studied during a 10 month period. Mean gestational age of the babies was 29.01 weeks and the mean birth weight was 1.728 kg. Escherichia coli and group B streptococci (GBS) colonized the umbilicus of 7 and 6 babies respectively, out of 23 studied, on the first day of life. E. coli and staphylococci were the predominant flora on the 6th day and they colonized 12 and 13 respectively. The oral flora was predominantly Gram-positive cocci, mainly Streptococcus salivarius which was isolated from 17 out of 22 babies on the 6th day, viridans streptococci were isolated from 14 babies, Staphylococcus albus from 16 babies and group D streptococci from 11 babies. Candida spp. also colonized the oral cavities of 17 out of 22 babies on the 6th day. At the end of the first week of life, the faecal flora was predominantly anaerobic represented by Bifidobacterium spp., Bacteroides spp. and Clostridium spp. The commonest facultative faecal flora were E. coli, which was isolated from all the babies, and Strept. faecalis isolated from 20 babies. Early gut colonization by GBS, Bacteroides spp. and Clostridium spp. was noticed in more babies delivered vaginally than by caesarean section where colonization by these bacteria was relatively delayed. The use of prophylactic penicillin plus gentamicin in the special neonatal unit probably prevented systemic spread of any of the potential opportunistic pathogens during the study.

STUDIES IN THE COMMON COLD : I. OBSERVATIONS OF THE NORMAL BACTERIAL FLORA OF NOSE AND THROAT WITH VARIATIONS OCCURRING DURING COLDS.

Shibley, G. S.; Hanger, F. M.; Dochez, A. R.;
Fonte: The Rockefeller University Press Publicador: The Rockefeller University Press
Tipo: Artigo de Revista Científica
Publicado em 28/02/1926 EN
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1. The normal bacterial flora of the nose and throat of thirteen individuals has been studied over periods ranging from 5 to 9 months. 2. Observations have been made of qualitative and quantitative changes in the flora occurring in the course of colds and of throat infections appearing in the group. 3. The normal basic nasal flora includes Staphylococcus albus, diphtheroids, and for certain individuals Staphylococcus aureus and citreus. Occasional transient bacteria are Gram-negative cocci and non-hemolytic streptococci. 4. The normal basic throat flora includes Gram-negative cocci, non-hemolytic streptococci, and for certain individuals "large Gram-positive cocci," B. influenzæ, Bacillus "X," and diphtheroids. Transient organisms are Staphylococcus albus, hemolytic streptococci,Staphylococcus aureus and citreus, and pneumococci. 5. No bacteria were found in early cold cultures to which a causative rôle could be assigned. 6. In the course of colds the basic flora of the nose was often scanty in the early stages. The throat showed reduction of prominence or alterations in predominance of the basic flora. 7. Certain organisms were prominent in colds, usually as late or secondary invaders, these included Staphylococcus aureus, hemolytic streptococci...

Evolution of bacterial flora in burn wounds: key role of environmental disinfection in control of infection

Taneja, Neelam; Chari, PS; Singh, Malkit; Singh, Gagandeep; Biswal, Manisha; Sharma, Meera
Fonte: e-Century Publishing Corporation Publicador: e-Century Publishing Corporation
Tipo: Artigo de Revista Científica
Publicado em 18/04/2013 EN
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Bacterial flora in burn patients undergoes change over period of time and is dependent upon many factors. Study of burn flora is not only helpful in locating entry of multidrug resistant bacterial strains into the unit’s usual flora but also in determining current antibiotic susceptibilities. Since no studies are available from India that have studied sequential emergence of different microorganisms in burn wound, present study was carried out to study evolution of bacterial flora in burn wounds and its correlation with invasive wound infection. Environmental sampling was also carried out for possible sources of infection. Patients with 20-70% of total burn surface were enrolled and followed up for entire duration of stay. Clinical & treatment details were noted. Surface wound swabs were collected on first, third, seventh, tenth and fourteenth day post admission. Environmental sampling was done every three months. Of 215 wound swabs collected from 71 patients, 72 were sterile and 143 yielded 214 isolates. Colonization rates were 33% on first day, 94% on 7th day and 100% by 14th day. 42% swabs grew gram negative bacteria. Overall Staphylococcus aureus was the predominant isolate (45%) followed by Pseudomonas aeruginosa (13.9%), beta hemolytic Streptococci (9.4%). Maximum invasive infections were seen at the seventh day. A high level of environmental contamination was seen with S. aureus...

Bioleaching of Arsenic-Rich Gold Concentrates by Bacterial Flora before and after Mutation

Xie, Xuehui; Yuan, Xuewu; Liu, Na; Chen, Xiaoguang; Abdelgadir, Awad; Liu, Jianshe
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
EN
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In order to improve the bioleaching efficiency of arsenic-rich gold concentrates, a mixed bacterial flora had been developed, and the mutation breeding method was adopted to conduct the research. The original mixed bacterial flora had been enrichedin acid mine drainage of Dexing copper mine, Jiangxi Province, China. It was induced by UV (ultraviolet), ultrasonic, and microwave, and their combination mutation. The most efficient bacterial flora after mutation was collected for further bioleaching of arsenic-rich gold concentrates. Results indicated that the bacterial flora after mutation by UV 60 s combined with ultrasonic 10 min had the best oxidation rate of ferrous, the biggest density of cells, and the most activity of total protein. During bioleaching of arsenic-rich gold concentrates, the density of the mutant bacterial cells reached to 1.13 × 108 cells/mL at 15 days, more than 10 times compared with that of the original culture. The extraction of iron reached to 95.7% after 15 days, increased by 9.9% compared with that of the original culture. The extraction of arsenic reached to 92.6% after 12 days, which was increased by 46.1%. These results suggested that optimum combined mutation could improve leaching ability of the bacterial flora more significantly.

Effects of Bacillus subtilis KN-42 on Growth Performance, Diarrhea and Faecal Bacterial Flora of Weaned Piglets

Hu, Yuanliang; Dun, Yaohao; Li, Shenao; Zhao, Shumiao; Peng, Nan; Liang, Yunxiang
Fonte: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) Publicador: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
Tipo: Artigo de Revista Científica
Publicado em /08/2014 EN
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This research focused on the effects of different doses of Bacillus subtilis KN-42 on the growth performance, diarrhea incidence, faecal bacterial flora, and the relative number of Lactobacillus and Escherichia coli in faeces of weaned piglets to determine whether the strain can serve as a candidate antimicrobial growth promoter. A total of 360 piglets (initial body weight 7.14±0.63 kg) weaned at 26±2 days of age were randomly allotted to 5 treatment groups (4 pens per treatment with 18 pigs per pen) for a 28-day trial. Dietary treatments were basal diet without any antimicrobial (negative control; NC), basal diet supplemented with 120 mg/kg feed of neomycin sulfate (positive control; PC) and basal diet supplemented with 2×109 (L), 4×109 (M) and 20×109 (H) CFU/kg feed of B. subtilis KN-42. During the overall period, average daily gain and feed efficiency of piglets were higher in groups PC, M, and H than those in group NC (p<0.05), and all probiotics and antibiotics groups had a lower diarrhea index than group NC (p<0.05). The 16S rDNA gene-based methods were used to analyze faecal bacterial flora on day 28 of experiment. The result of denaturing gradient gel electrophoresis analysis showed that supplementation of B. subtilis KN-42 to the diet changed the bacterial communities...

Estudo da flora bacteriana de Gram-positivo das mãos dos alunos de Enfermagem integrados nos ensinos clínicos e os conhecimentos sobre a higiene das mãos

Ribeiro, Sílvia Patrícia Guimarães
Fonte: [s.n.] Publicador: [s.n.]
Tipo: Trabalho de Conclusão de Curso
Publicado em //2011 POR
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Trabalho apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Licenciada em Enfermagem; A falta de higiene das mãos ou a sua má prática constitui o principal motivo de desenvolvimento de infecções associadas aos cuidados de saúde (IACS). Para além do aumento substancial da mortalidade dos doentes internados em contexto hospitalar, as IACS constituem uma das maiores causas de morte em todo o mundo. Tendo noção da importância que a higiene das mãos constitui na actualidade desenvolveu-se o presente Projecto de Graduação subordinado ao tema “Estudo da flora bacteriana de Gram-positivo das mãos dos alunos de Enfermagem integrados nos ensinos clínicos e os conhecimentos sobre a higiene das mãos”. Este teve como principais objectivos caracterizar a flora bacteriana de Gram-positivo presente nas mãos dos alunos do CLE da UFP integrados nos EC, identificar o conhecimento dos alunos do CLE da UFP integrados nos EC acerca da higiene das mãos e descrever a relação entre a flora bacteriana de Gram-positivo das mãos dos alunos do CLE da UFP integrados nos EC e os conhecimentos sobre a higiene das mãos. Neste sentido, a investigação desenvolvida pretendeu dar resposta às questões: Qual a flora bacteriana de Gram-positivo das mãos dos alunos do CLE da UFP integrados nos EC?...

Evaluation of conjunctival bacterial flora in patients with Stevens-Johnson Syndrome

Frizon,Luciana; Araujo,Marilia Cavalcante; Andrade,Larissa; Yu,Maria Cecilia Zorat; Wakamatsu,Tais Hitomi; Hofling-Lima,Ana Luisa; Gomes,Jose Alvaro Pereira
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2014 EN
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OBJECTIVE: To determine the conjunctival bacterial flora present in patients with Stevens-Johnson syndrome. METHODS: A prospective study of the conjunctival bacterial flora was performed in 41 eyes of 22 patients with Stevens-Johnson syndrome. The information gathered included the patient's sex and age, the duration of disease, the cause of Stevens-Johnson syndrome, and treatments. Scrapings of the inferior conjunctival fornix were performed in both eyes. Fourteen days before scraping, the patients were asked to interrupt all topical medication and start using 0.5% nonpreserved methylcellulose. The microbiological evaluation included microorganism identification and determination of antibiotic sensitivity. RESULTS: Of 22 patients (41 eyes), 14 (64%) were females, and eight (36%) were males. The mean age was 33.2 years, and the mean duration of disease was 15.6 years. Visual acuity ranged from light perception to 20/25 (1.57 logMar). The treatment received by most patients consisted of tear substitutes, topical antibiotics, and contact lenses. Bacterial identification was positive in 39 eyes (95%) and negative in two eyes (5%). Gram-positive cocci accounted for 55.5% of the microorganisms, whereas gram-positive bacilli and gram-negative bacilli accounted for 19% and 25.5%...

I microbi dell'ape regina (Apis mellifica L.) — La flora microbica del mesointestino

Vecchi, Maria Adelaide; Zambonelli, Carlo
Fonte: INRA - Instituto Nacional de Investigação Agronômica da França Publicador: INRA - Instituto Nacional de Investigação Agronômica da França
Tipo: Journal Article-postprint
IT; ENGLISH
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The bacterial flora of the mid-gut of a lot of 100 queen-bees in normal health conditions and taken from different hives was investigated. From 44 queens, 62 isolates of aerobic bacteria were obtained: among them 41 strains were recognized as representatives of the genus Achromobacter while other 18 belong undoutebly to the genus Brevibacterium. The frequency of their incidence in the midguts, the diversity in hosts provenience and the largerly variable conditions of their sampling and expedition are elements sufficient to warrant the conclusion that the bacteria are not harboured incidentally in the mid-gut of these insects but they may multiply although moderately or at least survive in this habitat without any damage fr host organism.

Evaluation of conjunctival bacterial flora in patients with Stevens-Johnson Syndrome

Frizon, Luciana; Araujo, Marilia Cavalcante; Andrade, Larissa; Yu, Maria Cecilia Zorat; Wakamatsu, Tais Hitomi; Hofling-Lima, Ana Luisa; Gomes, Jose Alvaro Pereira
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/03/2014 ENG
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46.41%
OBJECTIVE: To determine the conjunctival bacterial flora present in patients with Stevens-Johnson syndrome. METHODS: A prospective study of the conjunctival bacterial flora was performed in 41 eyes of 22 patients with Stevens-Johnson syndrome. The information gathered included the patient's sex and age, the duration of disease, the cause of Stevens-Johnson syndrome, and treatments. Scrapings of the inferior conjunctival fornix were performed in both eyes. Fourteen days before scraping, the patients were asked to interrupt all topical medication and start using 0.5% nonpreserved methylcellulose. The microbiological evaluation included microorganism identification and determination of antibiotic sensitivity. RESULTS: Of 22 patients (41 eyes), 14 (64%) were females, and eight (36%) were males. The mean age was 33.2 years, and the mean duration of disease was 15.6 years. Visual acuity ranged from light perception to 20/25 (1.57 logMar). The treatment received by most patients consisted of tear substitutes, topical antibiotics, and contact lenses. Bacterial identification was positive in 39 eyes (95%) and negative in two eyes (5%). Gram-positive cocci accounted for 55.5% of the microorganisms, whereas gram-positive bacilli and gram-negative bacilli accounted for 19% and 25.5%...

AVALIAÇÃO DA EFICIÊNCIA DE ANTISSÉPTICOS NA LIMPEZA DAS MÃOS; EVALUATION OF ANTISEPTIC EFFICIENCY IN HANDS CLEANING

SILVEIRA MARTINS, SUZANA CLÁUDIA; Universidade Federal do Ceará; SOARES, JUAREZ BRAGA; Universidade Federal do Ceará
Fonte: UFPR Publicador: UFPR
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Formato: application/pdf
Publicado em 12/05/2009 POR
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Avalia a eficiência bactericida de dois antissepticos (solução de etanol e álcool isopropÍlico e solução de. polivinilpirrolidona-iodo - com 10% de iodo ativo) e do sabão em barra, na flora bacteriana cutânea das mãos. Grupos de pessoas foram submetidas à lavagem das mãos com os agentes citados- durante certo período de tempo, através da fricção de uma das mãos, numa área de 30 cm', fazendo-se posterior- contagem em placas e relacionando-se- tal determinação com a flora da outra mão não tratada com os antissépticos.; The purpose of this paper was to evaluate the bactericidal efficiency of two antiseptics (ethyl and isopropyl alcohol solution and polyvinilpirrolidine - iodine solution) against soup on the bacterial flora of the skin. The region treated was the hand of 18 people, where the antiseptics were applied for one minute. The percentage of bacterial reduction were 99,4% for alcohol solution, 98,7% for PVP-I and 83,3% for soap.