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Evaluation of anti-inflammatory activity of derivatives from aerial parts of Baccharis uncinella

ZALEWSKI, Cynthia A.; PASSERO, Luiz Felipe D.; MELO, Alexis S. R. B.; CORBETT, Carlos Eduardo P.; LAURENTI, Marcia D.; TOYAMA, Marcos H.; TOYAMA, Daniela O.; ROMOFF, Paulete; FAVERO, Oriana A.; LAGO, Joao Henrique G.
Fonte: INFORMA HEALTHCARE Publicador: INFORMA HEALTHCARE
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
66.15%
Context: Species of Baccharis exhibit antibiotic, antiseptic, and wound-healing properties, and have been used in the traditional medicine of South America for the treatment of inflammation, headaches, diabetes, and hepatobiliary disorders. Objective: To investigate the anti-inflammatory activity of organic phases from EtOH extract of the aerial parts of Baccharis uncinella DC (Asteraceae). Materials and methods: The crude EtOH extract from the aerial parts of B. uncinella was subjected to partition procedures and the corresponding CH(2)Cl(2) and EtOAc phases were subjected to several chromatographic separation procedures. Thus, these phases and their purified compounds were assayed for evaluation of anti-inflammatory activity. Results: The CH(2)Cl(2) phase from EtOH extract from B. uncinella contained two triterpenoids (oleanolic and ursolic acids) and one flavonoid (pectolinaringenin), whereas the respective EtOAc phase showed to be composed mainly by two phenylpropanoid derivatives (caffeic and ferulic acids). The CH(2)Cl(2) and EtOAc phases as well as their isolated compounds exhibited anti-inflammatory effects against inflammatory reactions induced by phospholipase A2 (from Crotalus durissus terrificus venom) and by carrageenan. Discussion and conclusion: The results suggested that the components obtained from partition phases of EtOH extract of B. uncinella could represent lead molecules for the development of anti-inflammatory agents. Additionally...

Atividade anti-inflamatória e caracterização fitoquímica do chá e de diferentes extratos de Tithonia diversifolia (Asteraceae); Anti-inflammatory activity and phytochemical characterization of infuse and different extracts from Tithonia diversifolia (Asteraceae)

Paula, Daniela Aparecida Chagas de
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 04/10/2010 PT
Relevância na Pesquisa
66.25%
Tithonia diversifolia Hemsl. A. Gray (margaridão, Asteraceae) é uma planta medicinal com propriedade anti-inflamatória bastante promissora, embora tenha sido parcialmente investigada do ponto de vista químico e farmacológico. Neste trabalho, a partir de suas folhas foram obtidos um infuso (chá), um extrato de lavagem foliar em acetona rico em lactonas sesquiterpênicas (LSTs), um extrato em metanol-H2O das folhas livres de tricomas (ELT) e o óleo essencial, incluindo das inflorescências. Pela primeira vez os constituintes polares das folhas de T. diversifolia foram identificados e o seu mecanismo de ação anti-inflamatório foi investigado. Através dos perfis químicos dos extratos obtidos por CLAE-UV-DAD e CG-EM, comparação com dados da literatura e de uma biblioteca de padrões, juntamente com a identificação de alguns constituintes isolados, foi possível identificar mais de 20 substâncias desta planta e efetuar a caracterização química de todos seus extratos. O sucesso na obtenção de extratos que refletem diferentes classes de metabólitos secundários de T. diversifolia, avaliados por ensaios anti-inflamatórios in vivo (tópico e oral) e in vitro, permitiu concluir que as LSTs não são as únicas substâncias que contribuem para atividade anti-inflamatória da planta como se acreditava anteriormente. O extrato polar (ELT)...

Avaliação do processo de reparo tecidual em tendão de Aquiles de ratos após indução de tendinopatia por colagenase: efeito do laser de baixa intensidade e de drogas anti-inflamatórias.; Evaluation of tissue repair process in rats Achilles tendon after tendinopathy induced by collagenase: effect of low-level laser and anti-inflammatory drugs.

Rossi, Rafael Paolo
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 23/08/2011 PT
Relevância na Pesquisa
66.15%
Introdução: A tendinopatia de Aquiles caracteriza-se pela presença de sinais clássicos da resposta inflamatória, como o edema e a dor, além de alterações estruturais no tecido. Neste estudo avaliamos o processo de reparação e reorganização tecidual de tendões de ratos submetidos a tendinopatia por colagenase, sendo posteriormente tratados com laserterapia de baixa intensidade, anti-inflamatório esteroidal e anti-inflamatório não-esteroidal. Materiais e Método: Ratas Wistar fêmeas foram submetidas a injeção transcutânea de colagenase (100mg/tendão) na região peritendínea das patas e posteriormente divididas e tratadas nos seguintes grupos: laser (3J), diclofenaco potássico (1,1mg/kg) e dexametasona (0,02mg/kg). Foram realizadas análises histomorfológicas, incluindo o score de achados, quantidade de colágeno presente no tecido e nível de agregação/organização das fibras colágenas. Resultados e Discussão: A colagenase produziu o aumento de células inflamatórias, extravasamento plasmático e hemorragia. Entre os tratamentos testados, o laser mostrou-se a melhor opção, atenuando a resposta inflamatória, aumentando a concentração de colágeno e mantendo a organização das fibras colágenas.; Introduction: Achilles tendinopathy is characterized by presence of classical signs of inflammatory response such as edema...

Avaliação de sintomas e lesões esôfago-gastroduodenais secundários ao uso de antiinflamatórios; Evaluation of symptoms and esophageal-gastroduodenal lesions, secondary to the use of anti-inflammatory drugs

Dib, Ricardo Anuar
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 22/08/2013 PT
Relevância na Pesquisa
66.23%
Introdução: Os antiinflamatórios não esteróides (AINEs), incluindo a aspirina, são drogas largamente utilizadas para tratamento das doenças inflamatórias e da dor, e que podem causar efeitos colaterais sérios, causando considerável morbidade e mortalidade, relacionadas á doença ulcerosa, duodenal e gástrica, particularmente ao sangramento gastrointestinal. O risco relativo global de complicações gastroduodenais é de três a dez vezes, maior nos usuários de AINEs, quando comparado com indivíduos sadios. Cerca de 25% dos usuários crônicos dos antiinflamatórios não esteroides (AINEs) deverão desenvolver doença ulcerosa, e de 2 a 4% deverão apresentar sangramento ou perfuração. Mais de 17.000.000 de norte americanos utilizam vários tipos de drogas antiinflamatórios não esteróides (AINEs) diariamente e que provocam mais de 100.000 hospitalizações e cerca de 7000 a 10.000 mortes por ano nos Estados Unidos da América do Norte, fazendo desta família de drogas uma das mais comumente usadas em todo planeta. Cerca de 50% das lesões observadas em endoscopias de controle, ocorrem sem que o paciente tenha qualquer tipo de sintoma. Acredita-se que houve recrudescimento da prevalência de lesões digestivas pela substituição dos antiinflamatórios COX-2 pelos antiinflamatórios tradicionais...

Atividade anti-inflamatória de extrato fenólico de tomate roxo (Solanum Lycopersicum L.) em camundongo em modelo de peritonite induzido pelo LPS; Anti-inflammatory activity of phenolic extract of purple tomato (Solanum lycopersicum L.) in mouse model of peritonitis induced by LPS.

Maia, Afonso Pinho da Silva
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 11/03/2015 PT
Relevância na Pesquisa
66.19%
Visando a produção de um alimento que possua elevados teores de compostos bioativos, a piramidação de genes é uma técnica capaz de estimular o acúmulo e a expressão de novas classes de flavonoides em tecidos vegetais, como por exemplo, o tomate roxo, rico em antocianinas. As antocianinas podem atenuar o processo inflamatório através da modulação da cascata de sinalização e da expressão de enzimas, sendo este um dos possíveis mecanismos de ação que leva a promoção da saúde, atribuído a esta classe de compostos. Assim, o objetivo deste trabalho foi avaliar a ação anti-inflamatória do extrato de tomate roxo, obtido por piramidação dos genes Anthocyanin Fruit (Aft), Aubergine (Abg) e atroviolaceum (atv), em camundongos submetidos ao modelo de peritonite induzida por lipopolissacarídeo (LPS). O fruto tomate vermelho - Micro Tom (MT) e o transformado foram caracterizados quanto ao seu perfil de compostos fenólicos. A casca do tomate roxo, rica em antocianinas, apresentou conteúdo de fenólicos totais dez vezes maior quando comparado à casca do MT, apresentando também maiores quantidades de ácido ascórbico e capacidade antioxidante avaliado nos métodos DPPH e ORAC; em relação à polpa e casca do tomate vermelho e a polpa do tomate roxo. Os principais flavonoides identificados na casca do tomate roxo...

Evaluation of anti-inflammatory activity of derivatives from aerial parts of Baccharis uncinella

Zalewski, Cynthia A.; Passero, Luiz Felipe D.; Melo, Alexis S. R. B.; Corbett, Carlos Eduardo P.; Laurenti, Marcia D.; Toyama, Marcos H.; Toyama, Daniela O.; Romoff, Paulete; Favero, Oriana A.; Lago, Joao Henrique G.
Fonte: Informa Healthcare Publicador: Informa Healthcare
Tipo: Artigo de Revista Científica Formato: 602-607
ENG
Relevância na Pesquisa
66.15%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Context: Species of Baccharis exhibit antibiotic, antiseptic, and wound-healing properties, and have been used in the traditional medicine of South America for the treatment of inflammation, headaches, diabetes, and hepatobiliary disorders.Objective: To investigate the anti-inflammatory activity of organic phases from EtOH extract of the aerial parts of Baccharis uncinella DC (Asteraceae).Materials and methods: The crude EtOH extract from the aerial parts of B. uncinella was subjected to partition procedures and the corresponding CH(2)Cl(2) and EtOAc phases were subjected to several chromatographic separation procedures. Thus, these phases and their purified compounds were assayed for evaluation of anti-inflammatory activity.Results: The CH(2)Cl(2) phase from EtOH extract from B. uncinella contained two triterpenoids (oleanolic and ursolic acids) and one flavonoid (pectolinaringenin), whereas the respective EtOAc phase showed to be composed mainly by two phenylpropanoid derivatives (caffeic and ferulic acids). The CH(2)Cl(2) and EtOAc phases as well as their isolated compounds exhibited anti-inflammatory effects against inflammatory reactions induced by phospholipase A2 (from Crotalus durissus terrificus venom) and by carrageenan.Discussion and conclusion: The results suggested that the components obtained from partition phases of EtOH extract of B. uncinella could represent lead molecules for the development of anti-inflammatory agents. Additionally...

Anti-inflammatory activity of Lychnophora passerina, Asteraceae (Brazilian arnica)

Capelari-Oliveira, P.; Paula, C. A.; Rezende, S. A.; Campos, F. T.; Grabe-Guimarães, A.; Lombardi, J. A.; Saúde-Guimarães, D. A.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 393-398
ENG
Relevância na Pesquisa
66.23%
Ethnopharmacological relevance: Lychnophora passerina (Asteraceae), popularly known as arnica, is used to treat inflammation, pain, rheumatism, contusions, bruises and insect bites in Brazilian traditional medicine. Materials and methods: The anti-inflammatory activity of crude ethanolic extract of aerial parts of L. passerina and its ethyl acetate and methanolic fractions had their abilities to modulate the production of NO, TNF-α and IL-10 inflammatory mediators in LPS/IFN-γ-stimulated J774.A1 macrophages evaluated. Moreover, the crude ethanolic extract and derived fractions were also in vivo assayed by carrageenan-induced paw oedema in mice. Results: In vitro assays showed remarkable anti-inflammatory activity of L. passerina crude ethanolic extract (EE) and its ethyl acetate (A) and methanolic (M) fractions, through the inhibition of production of NO and TNF-α inflammatory mediators and induction of production of IL-10 anti-inflammatory cytokine. In vivo assays showed anti-inflammatory activity for EE 10% ointment, similar to the standard drug diclofenac gel. The A and M fraction ointments 20% presented anti-inflammatory activity. Conclusion: The results obtained showed that possible anti-inflammatory effects of EE and its A and M fractions may be attributed to inhibition pro-inflammatory cytokines production...

Pharmacological Evaluation and Preparation of Nonsteroidal Anti-Inflammatory Drugs Containing an N-Acyl Hydrazone Subunit

Ferreira de Melo, Thais Regina; Chelucci, Rafael Consolin; Lopes Pires, Maria Elisa; Dutra, Luiz Antonio; Barbieri, Karina Pereira; Bosquesi, Priscila Longhin; Goulart Trossini, Gustavo Henrique; Chung, Man Chin; Santos, Jean Leandro dos
Fonte: Mdpi Ag Publicador: Mdpi Ag
Tipo: Artigo de Revista Científica Formato: 5821-5837
ENG
Relevância na Pesquisa
66.16%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 11/15204-5; Processo FAPESP: 12/50359-2; A series of anti-inflammatory derivatives containing an N-acyl hydrazone subunit (4a-e) were synthesized and characterized. Docking studies were performed that suggest that compounds 4a-e bind to cyclooxygenase (COX)-1 and COX-2 isoforms, but with higher affinity for COX-2. The compounds display similar anti-inflammatory activities in vivo, although compound 4c is the most effective compound for inhibiting rat paw edema, with a reduction in the extent of inflammation of 35.9% and 52.8% at 2 and 4 h, respectively. The anti-inflammatory activity of N-acyl hydrazone derivatives was inferior to their respective parent drugs, except for compound 4c after 5 h. Ulcerogenic studies revealed that compounds 4a-e are less gastrotoxic than the respective parent drug. Compounds 4b-e demonstrated mucosal damage comparable to celecoxib. The in vivo analgesic activities of the compounds are higher than the respective parent drug for compounds 4a-b and 4d-e. Compound 4a was more active than dipyrone in reducing acetic-acid-induced abdominal constrictions. Our results indicate that compounds 4a-e are anti-inflammatory and analgesic compounds with reduced gastrotoxicity compared to their respective parent non- steroidal anti-inflammatory drugs.

Anti-inflammatory activities of the hydroalcoholic extracts from Erythrina velutina and E. mulungu in mice

Vasconcelos,Silvânia M. M.; Sales,Gabriel T. M.; Lima,Natália; Lobato,Rodrigo de Freitas G.; Macêdo,Danielle Silveira; Barbosa-Filho,José Maria; Leal,Luzia Kalyne A. M.; Fonteles,Marta M. F.; Sousa,Francisca Cléa F.; Oliveira,Jeania Lima; Viana,Glauc
Fonte: Sociedade Brasileira de Farmacognosia Publicador: Sociedade Brasileira de Farmacognosia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2011 EN
Relevância na Pesquisa
66.15%
This work studied the anti-inflammatory activities of the hydroalcoholic extracts (HAEs) from Erythrina velutina Willd. (Ev) and E. mulungu Mart. ex Benth. (Em) in the carrageenan- and dextran-induced mice hind paw edema models. These medicinal plants belonging to the Fabaceae family are used in some Brazilian communities to treat pain, inflammation, insomnia and disorders of the central nervous system. In the present work, the extracts were administered orally in male mice at the doses of 200 or 400 mg/kg. In the carrageenan-induced test, only Em showed anti-inflammatory activity, decreasing the paw edema, at the doses of 200 and 400 mg/kg. No effect was observed with Ev in this model. On the other hand, in the dextran model, Ev demonstrated anti-inflammatory effect, showing decrease of the paw edema at the 1, 2, 3, 4 and 24th h. Em (200 or 400 mg/kg) presented anti-inflammatory effect at the 2, 3 and 4th h after administration of dextran, as compared to control. In conclusion, the work showed that Ev and Em present anti-edematous actions, which possibly occurs by distinct mechanisms. While Ev seems to interfere especially in inflammatory processes in which mast cells have an important role, Em exerts greater activity in the inflammatory process that depends mainly on polymorphonuclear leucocytes. However...

Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propolis: a brief review

Araujo,Marcio A. R.; Libério,Silvana A.; Guerra,Rosane N. M.; Ribeiro,Maria Nilce S.; Nascimento,Flávia R. F.
Fonte: Sociedade Brasileira de Farmacognosia Publicador: Sociedade Brasileira de Farmacognosia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2012 EN
Relevância na Pesquisa
66.17%
Many biological properties have been attributed to various types of propolis, including anti-inflammatory, antimicrobial, antioxidant, antitumor, wound healing, and immunomodulatory activities. This article reviewed studies published that investigated the anti-inflammatory activity of propolis of different origins and/or its isolated components, focusing on the mechanisms of action underlying this activity and also addressing some aspects of immunomodulatory effects. The search was performed of the following databases: PubMed, Science Direct, HighWire Press, Scielo, Google Academics, Research Gate and ISI Web of Knowledgement. The anti-inflammatory activity was associated with propolis or compounds such as polyphenols (flavonoids, phenolic acids and their esters), terpenoids, steroids and amino acids. CAPE is the most studied compounds. The main mechanisms underlying the anti-inflammatory activity of propolis included the inhibition of cyclooxygenase and consequent inhibition of prostaglandin biosynthesis, free radical scavenging, inhibition of nitric oxide synthesis, reduction in the concentration of inflammatory cytokines and immunosuppressive activity. Propolis was found to exert an anti-inflammatory activity in vivo and in vitro models of acute and chronic inflammation and others studies...

Anti-inflammatory and antinociceptive effects of the hydroethanolic extract of the flowers of Pyrostegia venusta in mice

Veloso,Clarice C.; Cabral,Layla D. M.; Bitencourt,Andressa D.; Franqui,Lidiane S.; Santa-Cecília,Flávia V.; Dias,Danielle F.; Soncini,Roseli; Vilela,Fabiana C.; Giusti-Paiva,Alexandre
Fonte: Sociedade Brasileira de Farmacognosia Publicador: Sociedade Brasileira de Farmacognosia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2012 EN
Relevância na Pesquisa
66.15%
Pyrostegia venusta (Ker Gawl.) Miers, Bignoniaceae, is native to the Brazilian Cerrado and popularly known as "cipó-de-são-joão". In Brazilian folk medicine, the flowers of P. venusta are used as a general tonic and a treatment for diarrhea, vitiligo, cough, and common infections and inflammatory diseases of the respiratory system. Nevertheless, there are still no studies on its possible anti-inflammatory and antinociceptive effects. The P. venusta hydroethanolic extract (PvHE) was used to evaluate the anti-inflammatory and analgesic effects in carrageenan-induced paw edema, peritonitis induced by lipopolysaccharide, acetic acid-induced writhing, and formalin-induced paw-licking tests in Swiss male mice. PvHE at doses of 30-300 mg/kg p.o. demonstrated anti-inflammatory effect. PvHE reduced paw edema induced by carrageenan and inhibited leukocyte recruitment into the peritoneal cavity. The extracts showed antinociceptive activity in acetic acid-induced writhing and formalin tests. Our results showed that the PvHE demonstrated anti-inflammatory and antinociceptive action in mice. All the anti-inflammatory actions obtained are also suggested to due the presence of acacetin-7-O-β-glucopyranoside.

Avaliação da atividade antinociceptiva e antiinflamatória de novos derivados N-acilidrazônicos (NAH) pirazínicos.; Evaluation of antinociceptive and anti-inflammatory activity of new derivatives N-acilidrazônicos (NAH) pyrazine.

Silva, Yolanda Karla Cupertino da
Fonte: Universidade Federal de Alagoas; BR; Biologia; Programa de Pós-Graduação em Ciências da Saúde; UFAL Publicador: Universidade Federal de Alagoas; BR; Biologia; Programa de Pós-Graduação em Ciências da Saúde; UFAL
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
66.15%
A new series of derivatives N-acihidrazone (NAH) pirazinic was rationally planned be applied the strategy of simplification molecular on the unit quinoxalinic of prototype LASSBio-1018, identified as a potent anti-inflammatory and analgesic in LASSBio® designed by application the biososterism of rings on LASSBio-319. The new series of 21 derivatives were synthesized and subjected to experiments to determine the antinociceptive and anti-inflammatory activity. Were used mice of swiss line, of both sexes (20 to 35 g) and rats Wistar of both sexes (100-220 g). The substances were administered by p.o. (100 μmol/kg), except morphine which was administered pathway i.p. (15 μmol/kg). The indomethacin, celecoxib, thalidomide and morphine were used as standart drugs. For evaluation antinociceptive were performed the constriction abdominal assay, hyperalgesia induced-formalin and hot plate test. The anti-inflammatory action were investigated by the assay capsaicin-induced ear edema, peritonitis zymosan A-induced and assay of induction of syndrome arthritis experimental. On the constriction abdominal assay all compounds inhibited the constriction significantly standing out the prototypes LASSBio-1181 e LASSBio-1188 (97% and 86%) (p < 0...

How and by whom care is delivered influences anti-inflammatory use in asthma: Results of a national population survey

Adams, R.; Weiss, S.; Fuhlbrigge, A.
Fonte: Mosby Inc Publicador: Mosby Inc
Tipo: Artigo de Revista Científica
Publicado em //2003 EN
Relevância na Pesquisa
66.18%
BACKGROUND: Studies examining the influence of provider behavior and patterns of care delivery on the use of anti-inflammatory asthma therapy have been limited to selected populations or have been unable to assess the appropriateness of therapy for individuals. We have previously reported the influence of sociodemographic variables and asthma severity on reported use of asthma medications in the United States. Objective: We sought to examine the influence of patterns of care delivery and clinician behavioral factors on the use of anti-inflammatory medication by patients with asthma. METHODS: We performed a cross-sectional national random digit dial household telephone survey in 1998 of adult patients and parents of children with current asthma. Respondents were classified as having current asthma if they had a physician's diagnosis of asthma and were either taking medication for asthma or had asthma symptoms during the past year. Results: One or more persons met the study criteria for current asthma in 3273 (7.8%) households in which a screening questionnaire was completed. Of the 2509 persons (721 children <16 years of age) with current asthma interviewed, 507 (20.1%) reported current use of anti-inflammatory medication. In a multiple logistic regression model controlling for asthma symptoms...

Planejamento, síntese e avaliação farmacológica de novos candidatos a protótipos de fármacos anti-inflamatórios; Planning, synthesis and pharmacological evaluation of new candidates for prototype anti-inflammatory drugs

GOMES, Marcelo do Nascimento
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Ciências Farmacêuticas; Ciências da Saúde - Farmácia Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Ciências Farmacêuticas; Ciências da Saúde - Farmácia
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
66.16%
In the field of a research line that seeks the planning, the synthesis and the pharmacologycal evaluation of new candidates to prototypes of anti-inflammatory drugs, we will describe in this project the planning of derived new pyrazolics (LQFM 002-003), originally drawn starting from the nerolidilcatecol (24) and arylsulfonilpiperazines, (25) that present profile inhibition of the enzyme sPLA2. The compounds (E)-N-(3,7-dimethylocta-2,6-dienyl)-1,3-dimethtyl-1-H-pyrazol-5-amine (LQFM 002) and (E)-N-(3,7-dimethylocta-2,6-dienyl)-1,3-dimethyl-4-((4-methylpiperazin-1-il) methyl)-1H-pyrazol-5-amine (LQFM 003), were submitted to pharmacologycal rehearsals in vitro, seeking to evaluate the enzymatic inhibition activity of the sPLA2. For the prototype (LQFM 002) the inhibition halos were 14,43 ± 6,28%, 16,68 ± 2,45%, 23,61 ± 2,62%, 37,06 ± 3,25%, in the doses of 250, 500, 1000 and 2000 μg/mL respectively. For the compound (LQFM 003), the halos were 1,85 ± 1,38%, 9,29 ± 3,33%, 7,82 ± 3,32%, 13,21 ± 3,22%, respectively. Subsequently the rehearsal in vivo was accomplished to evaluate the profile of cellular migration. Being also analyzed the concentration of plasmatic protein by the methodology of the Evans of blue once the inflammatory process was induced. The compound (LQFM 002) presented inhibition on cellular migration of 50...

Modulação da via anti-inflamatória colinérgica no fígado e tecido adiposo branco da prole pelo consumo materno de dieta rica em gordura; Modulation of cholinergic anti-inflammatory pathway in the liver and white adipose tissue of the offspring by maternal high fat diet consumption

Tanyara Baliani Payolla
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 17/08/2015 PT
Relevância na Pesquisa
66.17%
A obesidade materna está relacionada ao desenvolvimento de distúrbios metabólicos na prole. O processo inflamatório desencadeado pelo consumo materno de dieta rica em gordura parece envolver a ativação da via TLR4/NFkB. O reflexo anti-inflamatório colinérgico é ativado por meio do nervo vago e através da subunidade α7 dos receptores nicotínicos de acetilcolina (α7nAChR) que reduz a ativação do NF-kB e diminui a produção de citocinas inflamórias e a intensidade da resposta imune. O bloqueio destes receptores ou a denervação da via eferente provoca intensa produção de mediadores inflamatórios em resposta ao lipopolissacarídeo (LPS). Neste sentido, estudos com modelos experimentais têm mostrado que a prole de mães obesas apresenta a resposta inflamatória exacerbada após um desafio com (LPS) ou dieta hiperlipídica (HFD). Assim, é possível que estes animais apresentem o controle do ajuste fino da resposta inflamatória afetado pelo consumo materno de HFD. O objetivo central foi avaliar a funcionalidade da via anti-inflamatória colinérgica (AIC) na prole de mães obesas. Para isso prole de camundongos swiss machos com 28 dias de vida (d28) obtidos de mães alimentadas com dieta HFD (HFD-O) ou dieta controle (SC-O) durante a gestação e lactação foram empregados no estudo. O LPS e a nicotina foram administrado via intraperitoneal (IP) e intracerebroventricular (i.c.v)...

In vivo and in vitro anti-inflammatory activity of red clover Trifolium pratense dry extract

Ramos,Graziele P.; Apel,Miriam A.; Morais,Cláudia B. de; Ceolato,Paula C.; Schapoval,Elfrides E. S.; Dall'Agnol,Miguel; Zuanazzi,José A. S.
Fonte: Sociedade Brasileira de Farmacognosia Publicador: Sociedade Brasileira de Farmacognosia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2012 EN
Relevância na Pesquisa
66.16%
Red clover Trifolium pratense L., Fabaceae, contains four isoflavones, mainly formononetin and biochanin A, and in smaller concentrations, daidzein and genistein. These compounds have gained a lot interest due to its human health benefits, such as estrogenic and progestogenic activities, antioxidant, anti-cancer and others. The objective of this study was to determine in vivo and in vitro anti-inflammatory activity of red clover dry extract. The in vitro anti-inflammatory activity was assayed by the technique using the Boyden chamber method, evaluating the leukocyte migration inhibition (chemotaxis). The in vivo anti-inflammatory activity was tested by a carrageenan-induced rat paw edema test. The results of anti-inflammatory in vitro test showed that there was a significant inhibition of leukocyte migration at the concentrations of 100, 50, 25, 10 and 5 µg/mL of red clover dry extract, these doses resulted in 94.73, 95.39, 94.73, 84.68 and 78.75% of inhibition for each dose, respectively. The anti-inflammatory in vivo test resulted in a significant activity in both tested doses (100 and 50 mg/kg of red clover dry extract) and at each tested time. The average percentage of edema inhibition was 63.37%. The findings of this study suggested that red clover extract might be suitable for the treatment of inflammatory diseases.

In vivo and in vitro anti-inflammatory activity of red clover Trifolium pratense dry extract

Ramos, Graziele Pereira; Apel, Miriam Anders; Morais, Cláudia Borges de; Ceolato, Paula Casagrande; Schapoval, Elfrides Eva Scherman; Dall'Agnol, Miguel; Zuanazzi, Jose Angelo Silveira
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
ENG
Relevância na Pesquisa
66.16%
Red clover Trifolium pratense L., Fabaceae, contains four isoflavones, mainly formononetin and biochanin A, and in smaller concentrations, daidzein and genistein. These compounds have gained a lot interest due to its human health benefits, such as estrogenic and progestogenic activities, antioxidant, anti-cancer and others. The objective of this study was to determine in vivo and in vitro antiinflammatory activity of red clover dry extract. The in vitro anti-inflammatory activity was assayed by the technique using the Boyden chamber method, evaluating the leukocyte migration inhibition (chemotaxis). The in vivo anti-inflammatory activity was tested by a carrageenan-induced rat paw edema test. The results of anti-inflammatory in vitro test showed that there was a significant inhibition of leukocyte migration at the concentrations of 100, 50, 25, 10 and 5 μg/mL of red clover dry extract, these doses resulted in 94.73, 95.39, 94.73, 84.68 and 78.75% of inhibition for each dose, respectively. The anti-inflammatory in vivo test resulted in a significant activity in both tested doses (100 and 50 mg/kg of red clover dry extract) and at each tested time. The average percentage of edema inhibition was 63.37%. The findings of this study suggested that red clover extract might be suitable for the treatment of inflammatory diseases.

Avaliação da atividade antinociceptiva e anti-inflamatória do extrato etanólico de Punica granatum L. (ROMÃ); Evaluation of antinociceptive and anti-inflammatory activities of Punica granatum ethanolic extract (POMEGRANATE)

Vieira, Ana Carolina Santana
Fonte: Universidade Federal de Alagoas; Brasil; Programa de Pós-Graduação em Enfermagem; UFAL Publicador: Universidade Federal de Alagoas; Brasil; Programa de Pós-Graduação em Enfermagem; UFAL
Tipo: Dissertação
POR
Relevância na Pesquisa
66.18%
Despite existing several pharmacological alternatives, it is growing the number of researchers interested in studying the pathophysiology and mediators of inflammation, because the drugs that are currently available on the market have limited efficiency and/ or have adverse effects that limit their use. Thus, it is necessary to continue the research in the search for more effective options. One alternative is the use of Punica granatum (pomegranate) that in the literature there are reports of the use of many of its parts in the treatment of infectious and inflammatory diseases. Considering the results obtained in previous studies on the actions of P. granatum and the need to increase the knowledge about their applications, this study aimed to evaluate the anti-nociceptive and anti-inflammatory potencial effects of ethanolic extract from fruits peels's Punica granatum (EEPG). Peels, from Maragogi-AL, were lyophilized, grinded, macerated in ethanol PA, filtered and the final product rotaevaporated, obtaining 65 g EEPG in which the phytochemical analysis revealed the presence of flavonoids, tannins and saponins. Toxicity tests were performed in vitro (MTT assay at doses of 1, 10 and 100 mg/ mL) and in vivo (acute toxicity test at doses of 500 and 1000 mg/ kg). To evaluate the antinociceptive activity were used as experimental models: writhing test induced by acetic acid...

Antinociceptive, anti-inflammatory and antipyretic effects of lapidin, a bicyclic sesquiterpene

Valencia, Emir; Feria, Manuel; Díaz, Jesús G.; González, Antonio G.; Bermejo, Jaime
Fonte: Thieme Publicador: Thieme
Tipo: Artículo Formato: 141452 bytes; application/pdf
ENG
Relevância na Pesquisa
66.16%
5 pages.-- PMID: 7997463 [PubMed].-- A preliminary account of some of these results has been presented at the I Simposio Internacional de Química de Productos Naturales y sus Aplicaciones (Universidad Técnica Federico Santa María, Valparaíso, Chile, Sep 24-26, 1992).; Lapidin, a bicyclic sesquiterpene from Ferula linkii Webb, has been evaluated for antinociceptive, anti-inflammatory, and antipyretic activities in mice and rats. Lapidin, vehicle, or the appropriate reference analgesic and anti-inflammatory compounds (acetylsalicylic acid [ASA] and indomethacin) were injected i.p. The more conspicuous effects of lapidin were: 1) a significant protective effect (17-62%; 2.5-20 mg/kg) towards writhings and stretchings induced by acetic acid in mice; 2) a significant antinociceptive effect on jumping responses in the hot-plate test (120 seconds at 15 mg/kg vs. 40 and 85 seconds in vehicle and ASA-treated mice, respectively); 3) a significant dose-dependent (5-15 mg/kg) antinociceptive effect against mechanical noxious stimuli; 4) a dose-dependent anti-inflammatory effect in the carrageen-an-induced edema which, at the higher dose (20 mg/kg), was comparable (65% inhibition of paw swelling at 3 hours) to that obtained after indomethacin (10 mg/kg); and 5) a moderate antipyretic effect against yeast-induced hyperthermia. Present results demonstrate that lapidin possesses antinociceptive...

Antinociceptive and Anti-inflammatory Effects of Caulerpa kempfii (Caulerpaceae); Efeito Antinociceptivo e Anti-inflamatório de Caulerpa kempfii (Caulerpaceae)

Carolina B. B. da Matta; Universidade Federal de Alagoas; Luiz Henrique A. Cavalcante-Silva; Universidade Federal de Alagoas; João Xavier de Araújo Júnior; Escola de Enfermagem e Farmácia; George Emmanuel C. de Miranda; Universidade Federal da Paraíb
Fonte: Revista Virtual de Química Publicador: Revista Virtual de Química
Tipo: ; Formato: binary/octet-stream
Publicado em 25/03/2015 PT
Relevância na Pesquisa
66.15%
Marine organisms are a rich source of new prototype drugs, and among them, the seaweeds are a very diverse group with different morphological, structural and metabolic features. The present work was designed to evaluate the anti-inflammatory and antinociceptive properties of the hexane (HE), ethyl acetate (EA) and hydroalcoholic (HA) fractions of Caulerpa kempfii in mice. The acetic acid-induced writhing, hot plate and formalin-induced nociception tests were carried out to evaluate the antinociceptive potential of these fractions, while the carrageenan-induced peritonitis test was used to investigate the anti-inflammatory activity of C. kempfii. In the acetic acid-induced writhing test, HE, EA, HA and dipyrone reduced the number of writhings by 76.7, 83.9, 90.8 and 89.3%, respectively. In the hot plate test, C. kempfii fractions did not increase the latency time of the animals in the time evaluated. In the neurogenic phase of the formalin test, the fractions significantly inhibited the pain response by 28.0% (HE), 37.4% (EA), and 35.9% (HA). While in the inflammatory phase, the inhibition was 55.1% (HE), 44.5% (EA) and 54.9% (HA), and indomethacin caused a 62.6% decrease in response. Moreover, in the carrageenan-induced peritonitis test...