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Genetically modified animals for use in research and biotechnology

CHAIBLE, Lucas Martins; CORAT, M. A; ABDELHAY, Eliana; DAGLI, Maria Lúcia Zaidan
Fonte: Ribeirão Preto Publicador: Ribeirão Preto
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
36.22%
Transgenic animals are used extensively in the study of in vivo gene function, as models for human diseases and in the production of biopharmaceuticals. The technology behind obtaining these animals involves molecular biology techniques, cell culture and embryo manipulation; the mouse is the species most widely used as an experimental model. In scientific research, diverse models are available as tools for the elucidation of gene function, such as transgenic animals, knockout and conditional knockout animals, knock-in animals, humanized animals, and knockdown animals. We examined the evolution of the science for the development of these animals, as well as the techniques currently used in obtaining these animal models. We review the phenotypic techniques used for elucidation of alterations caused by genetic modification. We also investigated the role of genetically modified animals in the biotechnology industry, where they promise a revolution in obtaining heterologous proteins through natural secretions, such as milk, increasing the scale of production and facilitating purification, thereby lowering the cost of production of hormones, growth factors and enzymes

Modelos de regeneração hepática em animais em crescimento: estudos histológicos, moleculares e avaliação de efeitos de imunossupressores; Experimental models of liver regeneration in growing animals. Histological and molecular studies, and evaluation of the effects of immunosuppressants

Tannuri, Ana Cristina Aoun
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 14/06/2007 PT
Relevância na Pesquisa
36.19%
INTRODUÇÃO: Transplantes parciais de fígado em crianças têm sido realizados com maior freqüência, enfatizando a importância do estudo da regeneração hepática, bem como dos efeitos de drogas imunossupressoras sobre a mesma. A regeneração do parênquima é o resultado do balanço entre multiplicação celular e apoptose, esta última definida como morte celular programada. Neste processo, estão envolvidas expressões de genes pró-apoptóticos (Bak e Bax) e anti-apoptóticos (Bcl-XL e Bcl-2). Dentre as proteínas relacionadas à proliferação hepatocitária, destaca-se a interleucina-6 (IL-6). Embora o modelo de ressecção de 70% da massa hepática de ratos adultos seja amplamente utilizado para estudos de regeneração, não há trabalhos com animais em crescimento. MÉTODOS: Na presente pesquisa, foi padronizado o modelo de hepatectomia parcial em ratos recém-nascidos e em recém-desmamados: realizou-se ligadura com fio de algodão do pedículo dos lobos esquerdo lateral, esquerdo medial e direito medial, seguida da ressecção do parênquima desses lobos. Os fígados remanescentes foram imediatamente pesados e comparados com os pesos dos fígados de animais controles. Para caracterização dos modelos de regeneração...

Conexão In Silico entre Plantas Medicinais e Animais Venenosos; In silico Connection between Medicinal Plants and Animals Venom

Puga, Renato David
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 15/04/2008 PT
Relevância na Pesquisa
36.17%
Na grande diversidade de plantas encontrada em todo o mundo, encontram-se as plantas medicinais com propriedades antivenenos. O estudo da relação dessas plantas com venenos de animais contribui muito para o desenvolvimento de novos medicamentos. A quantidade de dados a ser armazenada e a relação dessas informações é um processo que deve ser administrado por um sistema computacional. O desenvolvimento de sistemas de computadores tem se destacado nos últimos anos na Bioinformática e são muito úteis para organizar diferentes tipos de dados e, juntamente, com o uso de gerenciadores de conteúdo, eles contribuem, significantemente, no processo de desenvolvimento de softwares. O presente projeto teve como objetivo o desenvolvimento de um sistema computacional para Web, o qual relaciona dados de plantas medicinais com propriedades antivenenos e de animais venenosos, permitindo a integração dos mesmos, através de diferentes aplicativos de busca. O sistema foi denominado de Venom e está disponível no site http://gbi.fmrp.usp.br/venom/. Foram criadas categorias para a classificação dos dados de plantas e de animais. Essa categorização das informações é muito importante, pois possibilita o relacionamento das mesmas nas buscas por categorias. Os dados...

Animais humanos ou humanos animais?: um estudo sobre a representação dos animais antropomorfizados nos filmes de animação; Human animals or animals humans? A study on the representation of anthropomorphized in animated movies.

Vizachri, Tania Regina
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 07/10/2014 PT
Relevância na Pesquisa
36.17%
Atualmente as animações, antes destinadas somente ao público infantil, são sucesso de público inclusive entre adultos. Esses desenhos modernos têm atraído pela tecnologia, pela criatividade e pela mensagem que desejam passar. Crianças e até mesmo adultos são influenciados no imaginário por essas tramas. Raras são as animações que não utilizam animais como personagens principais que interagem com humanos ou que substituem completamente os próprios humanos. Nelas os animais são aproximados dos humanos tanto que até ganham voz e podem ser compreendidos por todos quanto aos seus interesses que, em algumas vezes, são mostrados de forma semelhante ao dos humanos. Adultos costumam compreender essas representações de animais como metáforas de nossas sociedades humanas. Então, nós podemos nos perguntar: antropomorfizar é eticamente correto? O que isso diz sobre nossa cultura? E sobre nossa relação com os animais? Tal dissertação objetiva investigar o que a representação desses animais antropomorfizados diz a respeito de nossa cultura, especialmente sobre o modo como vemos e lidamos com os animais.; Nowadays both, children and adults appreciate animated movies. Families do not need to have children to go to the movies to watch cartoons. These cartoons keep a high appeal due to their technology...

Detection of antibodies to Toxoplasma gondii in wild animals in Brazil

Silva, Rodrigo Costa Da; Machado, Gustavo Puglia; Cruvinel, Tatiane Morosini De Andrade; Cruvinel, Ciro Alexandre; Langoni, Helio
Fonte: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP Publicador: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Tipo: Artigo de Revista Científica Formato: 01-04
ENG
Relevância na Pesquisa
36.17%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite, Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008. Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10 (4; 66.7%) and 40 (2; 33.3%). The species Pseudalopex vetulus, Cerdocyon thous, Hydrochoerus hydrochaeris and Tapyrus terrestris had titers of 10, while Alouatta caraya and Puma concolor had titers of 40. There was no significant association regarding age, gender or purpose of care (p > 0.05). Conclusions Carnivorous...

Brazilian law for scientific use of animals

Marques,Ruy Garcia; Morales,Marcelo Marcos; Petroianu,Andy
Fonte: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia Publicador: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2009 EN
Relevância na Pesquisa
36.17%
The Brazilian scientific community claimed for a definitive systematization and for comprehensive and realistic national rules, to provide guidance and regulation, instead of sanctions, so that the question of scientific research involving animals could be better contemplated. This is beginning to occur now with Law n.º 11.794, sanctioned by the President of the Republic on November 8, 2008. PURPOSE: To describe the evolution of Brazilian regimentation for scientific use of animals and to analyze Law n.º 11.794. METHODS: The legislation about the use of animals in teaching and in scientific research in Brazil and in Rio de Janeiro State was identified and discussed. RESULTS: Until now, there was no updated general and systematizing rule regarding animal vivisection and experimentation for didactic or scientific purposes. The only specific law dates back to1979 and was not regimented. More recent laws equated the practice of scientific experiments to acts of abuse and mistreatment of animals, when alternative technology was available. Municipal laws that restricted the scientific practice of vivisection and experimentation with animals were created in the cities of Rio de Janeiro and Florianopolis. CONCLUSION: With the claim and collaboration of the scientific community...

Detection of antibodies to Toxoplasma gondii in wild animals in Brazil

Silva,Rodrigo Costa da; Machado,Gustavo Puglia; Cruvinel,Tatiane Morosini de Andrade; Cruvinel,Ciro Alexandre; Langoni,Helio
Fonte: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP Publicador: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2014 EN
Relevância na Pesquisa
36.17%
Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite,Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008. Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10 (4; 66.7%) and 40 (2; 33.3%). The species Pseudalopex vetulus, Cerdocyon thous, Hydrochoerus hydrochaeris and Tapyrus terrestris had titers of 10, while Alouatta caraya and Puma concolor had titers of 40. There was no significant association regarding age, gender or purpose of care (p > 0.05). Conclusions Carnivorous, herbivorous and omnivorous wild animals are potential sentinels of human toxoplasmosis, especially when wild felids are present, maintaining the environmental contamination.

An Examination of the Relationship between Humans and Animals in the Hebrew Bible

Windham, Mary
Fonte: Harvard University Publicador: Harvard University
Tipo: Thesis or Dissertation
EN_US
Relevância na Pesquisa
36.25%
The purpose of this dissertation is to explore the relationship between humans and animals in the Hebrew Bible. Although the Hebrew Bible contains a multitude of different perspectives on animals, I argue that there are two basic categories into which these perspectives fall. One group of biblical texts, most notably Gen 1:28, affirms humans’ dominion over animals. Despite the ubiquity of this perspective in scholarly literature, an examination of the entire biblical corpus suggests that these texts represent the minority. A larger group of biblical texts, most notably Job 39:9, depict animals as beyond the dominion of human beings. Rather than attempting to account for every mention of animals in the Hebrew Bible, I will examine a select group of passages that represent each of the two positions. Regarding passages that depict animals as under humans’ dominion, I conclude that while these passages suggest that humans have some authority over animals, that dominion is neither complete nor absolute. Regarding passages that depict animals as beyond humans’ dominion, I will conclude that they in fact indicate that animals are under the direct dominion of God. The Hebrew Bible nowhere grants humans absolute dominion over animals but instead typically presents humans as having little...

Behaving Like Animals: Human Cruelty, Animal Suffering, and American Culture, 1900-present

McGrath, Timothy Stephen
Fonte: Harvard University Publicador: Harvard University
Tipo: Thesis or Dissertation
EN_US
Relevância na Pesquisa
36.24%
What does it mean to be cruel to an animal? What does it mean for an animal to suffer? These are the questions embedded in the term "cruelty to animals," which has seemed, at first glance, a well defined term in modern America, in so far as it has been codified in anti-cruelty statutes. Cruelty to animals has been a disputed notion, though. What some groups call cruel, others call business, science, culture, worship, and art. Contests over the humane treatment of animals have therefore been contests over history, ideology, culture, and knowledge in which a variety of social actors-- animal scientists, cockfighters, filmmakers, FBI agents, members of Congress, members of PETA, and many, many others--try to decide which harms against animals and which forms of animal suffering are justifiable. Behaving Like Animals examines these contests in the United States from the beginning of the twentieth century to the present, focusing on four practices that modern American animal advocates have labeled cruel: malicious animal abuse, cockfighting, intensive animal agriculture, and the harming of animals on film. These case studies broadly trace the contours of American attitudes toward human cruelty and animal suffering over the last century. They also trace the historical evolution of the ideas embedded in the term “cruelty to animals.” Cruelty to animals has been the structuring logic of animal advocacy for two centuries...

Fungos e micoses em animais silvestres recebidos por Centros de Triagem; Fungi and mycosis in wild animals received by Screening Centers

Albano, Ana Paula Neuschrank
Fonte: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR Publicador: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
36.25%
The study of the infectious diseases in wild animals, in special the illnesses caused by fungi, have a few stories related with the incidence and distribution of the diverse ethiologic agents in captive populations and especially in the free ranging animals. The identification of the fungical species that are part of microbiota in healthful animals is primordial condition for the recognition of causers of pathological processes. The objective of this work was the isolation and the identification of fungi that is present in healthy wild animals or not, received in Screening Centers, and the respective study of mycosis caused by the fungi in wild animals in the states of the Rio Grande do Sul and Mato Grosso do Sul. The material collections had been carried through sterilized swabs for the external acoustic meatus and of the technique of "square of the carpet for the tegument of the wild animals in evaluation. The samples had been collected from 83 animals and the sorts of isolated fungi in this study had been Aspergillus sp., Candida spp., Penicillium sp., Geotrichum sp., Malassezia sp., Trichophyton sp., Trichophyton mentagrophytes, Fusarium sp. e Scopulariopsis sp. In 33 animals that had presented injuries it had fungical isolation in 97%. Amongst the birds...

Contacts with animals and humans as risk factors for adult brain tumours. An international case-control study

Menegoz, F.; Little, J.; Colonna, M.; Arslan, A.; Preston-Martin, S.; Schlehofer, B.; Blettner, M.; Howe, G.; Ryan, P.; Giles, G.; Rodvall, Y.; Choi, W.
Fonte: Pergamon-Elsevier Science Ltd Publicador: Pergamon-Elsevier Science Ltd
Tipo: Artigo de Revista Científica
Publicado em //2002 EN
Relevância na Pesquisa
36.2%
While numerous studies have addressed the possible role of farming and related exposures as risk factors for brain tumours in adults, few of them have examined the potential effect of exposure to farm animals or pets. In an international multicentre case–control study, we investigated whether residence on a farm, contact with animals, or working in occupations with a high degree of potential contact with animals or humans were associated with brain tumours. Using a common questionnaire, 1177 cases of glioma, 330 with meningioma and 2478 controls from eight centres were interviewed about the exposures and, in particular, about their contacts with nine species of animals: dairy cattle, beef cattle, pigs, horses, sheep, goats, poultry, dogs and cats. Living or working on a farm was not a risk factor, for either glioma or meningioma. Except in some centres, there was no relationship between having contacts with farm animals or pets and the risk of brain tumour, for either type of tumour or either sex. In relation to seven industrial groups involving frequent human and/or animal contacts, no association was apparent for either glioma or meningioma. In relation to 25 occupational groups with potential frequent contact with humans and/or animals...

The "Right to Autonomous Agency" and the "Right to Exit/ Sever Relationships": Theorizing our Obligations to Companion Animals in a Post-Animal Rights World

Roy, Shitangshu
Fonte: Quens University Publicador: Quens University
Tipo: Tese de Doutorado
EN; EN
Relevância na Pesquisa
36.23%
This thesis expands on the model presented in Sue Donaldson and Will Kymlicka’s Zoopolis for how companion animals might be treated as co-citizens in a post-Animal Rights world. I will attempt to clarify the distinct political obligations owed to companion animals throughout their lifetimes by individual caregivers and by the state. In particular, I argue that there is nothing in the genetic make-up of most companion animals that precludes them from being “autonomous agents” in adulthood, meaning that if allowed to develop their agency, most animals would be able to lead flourishing lives independent of human companions. I suggest that, for young companion animals, guardians have political obligations to develop the autonomous agency of their dependents, with help from the state. That said, for adult animals which develop autonomous agency, I argue that both a human caregiver and the animal have a right to sever their relationship with each other, just as we give adult children the right to leave their parents’ care and also give parents the “right to kick out” adult children who are capable of supporting themselves. However, while advocating for human caregivers’ rights to sever relationships with autonomous pets, I nonetheless maintain that the state will always retain obligations to its citizens to provide them with a basic level of welfare...

Animals, Animality, and Violence: Reading Across Species in J. M. Coetzee's Writing

Denike, Jaime
Fonte: Quens University Publicador: Quens University
Tipo: Tese de Doutorado
EN; EN
Relevância na Pesquisa
36.2%
This thesis examines the writings of Nobel Laureate J. M. Coetzee in order to explore pressing issues that have emerged in literary, philosophical, and theoretical approaches to animal studies. These include animals as disputed objects in claims to territorial, national, and cultural belonging; and the use of animality to manage cultural difference and mobilize identity-based violence. I investigate the roles that hierarchical discourses of species, and the rhetorics of animality that mobilize them, play in cultural and social inscription, cross-cultural conflict, and cultures of violence in the writing of J.M. Coetzee. My dissertation provides historical, material, and cultural context and specificity to the entanglements of race, gender, and culture with the rhetoric and hermeneutics of species, by demonstrating how colonial, Enlightenment, and traditional humanist thought mobilizes speciesism for the cultural work of violence. Intervening in assumptions about the irreconcilability of animal- and human-endorsing approaches to animal studies, I demonstrate that human and non-human animals alike are mutually implicated in conceptual economies that employ animality as a trope; and in the material logistics that mobilize discourses that surround nonhuman animals to do violence to human and nonhuman animals. Coetzee embeds questions about what nonhuman animals mean...

Students (ages 6, 10, and 15 years) in six countries knowledge of animals

Patrick, Patricia; Byrne, Jenny; Tunnicliffe, Sue Dale; Asunta, Tuula; Carvalho, Graça Simões de; Havu-Nuutinen, Sari; Sigurjónsdóttir, Hrefna; Óskarsdóttir, Gunnhildur; Tracana, Rosa Branca
Fonte: University of Oslo Publicador: University of Oslo
Tipo: Artigo de Revista Científica
Publicado em //2013 ENG
Relevância na Pesquisa
36.19%
This article considers the knowledge students (ages 6, 10, and 15 years) have of animals from a cross-cultural perspective. Students from six countries (Brazil, England, Finland, Iceland, Portugal, and the United States of America) were asked to free-list as many animals as possible and state where they had seen or learned about the animals. The results were analyzed and they indicate that 1) Students are aware of animals. 2) Students are more aware of mammals as examples of animals. 3) There is a globally shared folk biological knowledge of animals. 4) Students learn about animals during socio-cultural interactions. The educational implications are discussed.; CIEC – Research Centre on Child Studies, UM (FCT R&D 317)

Acute phase proteins in wild boar, Pyrenean chamois and Spanish ibex method validation and reference values /

Bach Iraich, Ester
Fonte: [Barcelona] : Universitat Autònoma de Barcelona, Publicador: [Barcelona] : Universitat Autònoma de Barcelona,
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em //2012 ENG; ENG
Relevância na Pesquisa
36.21%
Hi ha un grup de proteïnes plasmàtiques, anomenades proteïnes de fase aguda (PFAs), la concentració de les quals canvia quan es produeixen lesions tissulars induïdes per traumes o estrès i per condicions infeccioses i inflamatòries. La funció principal de les PFAs és recuperar la homeòstasi i limitar el creixement microbià. La quantificació de la concentració de les PFAs pot ser utilitzada com a eina pel diagnòstic i pronòstic, així com pel seguiment de tractaments. A la bibliografia hi ha molts articles que descriuen els diferents patrons de les proteïnes enfront diferents malalties. No obstant, la utilitat de les APPs i la seva dinàmica en malalties pròpies de poblacions salvatges no ha estat determinada. Els mètodes de laboratori que s'utilitzen actualment per mesurar les APPs s'haurien de validar abans de proporcionar cap resultat. Això és especialment important en les espècies salvatges, on les tècniques dels animals domèstics s'extrapolen sense validació prèvia, i per tant la fiabilitat dels resultats es desconeguda. Els objectius principals d'aquest treball són validar els mètodes analítics disponibles per la determinació de proteïnes de fase aguda en ungulats domèstics per al seu ús en ungulats salvatges...

Epidemiologia de la influença porcina estudis seroepidemiològics i dinàmica de la infecció en explotacions porcines /

Simon Grifé, Meritxell
Fonte: [Barcelona] : Universitat Autònoma de Barcelona, Publicador: [Barcelona] : Universitat Autònoma de Barcelona,
Tipo: Tesis i dissertacions electròniques; info:eu-repo/semantics/doctoralThesis Formato: application/pdf
Publicado em //2012 SPA; CAT
Relevância na Pesquisa
36.22%
A portada: Centre de Recerca en Sanitat Animal (CRESA); Des de l'any 1931, s'han aïllat diferents tipus de virus influença en l'espècie porcina i en l'actualitat es considera que certs subtipus circulen de forma endèmica entre la població porcina mundial. Degut a que els porcs poden infectar-se tant per virus influença d'origen aviar com d'origen humà, l'espècie porcina es considera la font de generació de nous virus influença recombinants, que podrien incloure gens de virus de diferents orígens. Per aquest motiu, més enllà de les conseqüències productives que comporta la malaltia pel sector porcí, la grip porcina prèn importància degut a les implicacions que pot tenir per la salut pública. En el primer estudi d'aquesta tesi es va examinar la seroprevalença enfront els virus influença en el porcí d'Espanya i els factors de risc associats. Es van recollir informació i mostres de sèrum (2.151 animals) de 98 explotacions distribuïdes arreu del país. Els resultats obtinguts mitjançant la tècnica d' inhibició de la hemaglutinació (IH), utilitzant els subtipus H1N1, H1N2 i H3N2 com antígens, van mostrar que el 75.4% dels animals presentaven anticossos enfront algun dels subtipus. A totes les granges estudiades es va detectar com a mínim un animal seropositiu enfront algun dels subtipus...

Epidemiological investigation of bovine tuberculosis causes of herd breakdowns and persistence in Spain

Guta Debela, Sintayehu
Fonte: [Bellaterra] : Universitat Autònoma de Barcelona, Publicador: [Bellaterra] : Universitat Autònoma de Barcelona,
Tipo: Tesis i dissertacions electròniques; info:eu-repo/semantics/doctoralThesis Formato: application/pdf
Publicado em //2013 ENG; ENG
Relevância na Pesquisa
36.17%
A Espanya, la prevalença de ramats amb tuberculosi ha disminuït del 11,1% el 1986 a 1,3% el 2011. Malgrat aquesta reducció en la prevalença, en els últims anys s'ha moderat el descens i l'actual situació epidemiològica de bTB planteja un desafiament cara a la consecució del estatus de país oficialment indemne de tuberculosi. Entre el 50 i el 60% dels ramats positius detecta cada any, són nous ramats positius, i la resta són ramats on la infecció persisteix des de l'any anterior. No es coneixen quines circumstàncies epidemiològiques condueixen a la introducció o la persistència de la infecció. Per tant, l'objectiu d'aquesta tesi és millorar el coneixement de l'epidemiologia de la bTB a Espanya mitjançant l'avaluació de les causes més probables d'infecció i els factors relacionats amb els ramats infectats de manera persistents. Per a l'estimació de les causes més probables d'introducció de la infecció en un ramat hem desenvolupat una metodologia basada en les diferents vies de transmissió de la bTB entre els ramats. S'han considerat set vies i per cada una s'ha realitzat un arbre de decisió sobre possibles riscos. A cada risc se l'hi ha assignat una probabilitat basada en opinió d'experts. Per a la gestió de dades i per al càlcul de les probabilitats de cada una de les diferents causes possibles s'han creat un parell de macros. D'acord amb els resultats dels 816 ramats estudiats (casos detectades principalment els anys 2010 i 2011)...

Sistemes de monitoratge per a cultius de cèl·lules animals adherents desenvolupament i aplicacions em models cel·lulars i tissulars

Sarró Casanovas, Enric
Fonte: [Barcelona] : Universitat Autònoma de Barcelona, Publicador: [Barcelona] : Universitat Autònoma de Barcelona,
Tipo: Tesis i dissertacions electròniques; info:eu-repo/semantics/doctoralThesis; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2010 CAT
Relevância na Pesquisa
36.2%
El cultiu de cèl·lules animals és una tecnologia que disposa d'un gran potencial econòmic i social, cada cop més elevat degut a la pròpia importància de les aplicacions en les que es troben relacionats aquests productes, que es troba enfocat al descobriment, desenvolupament i ús de nous productes biotecnològics. Degut a la major complexitat del cultiu de cèl·lules animals i a les limitacions que presenten envers els cultius de cèl·lules procariotes per a l'obtenció d'elevades concentracions cel·lulars, és necessari dotar el cultiu de cèl·lules animals de tecnologies que permetin optimitzar‐ne la seva expansió cel·lular i poder evitar l'actual suboptimització de la majoria dels processos de cultius de cèl·lules animals, que s'han basat en estratègies de cultiu simples (discontinu) i que limiten fortament les concentracions finals obtingudes. Un dels paràmetres clau en un cultiu de cèl·lules és la concentració cel·lular, essent la màxima concentració cel·lular un dels objectius primordials dels cultius cel·lulars a la fi de poder obtenir la màxima productivitat. No és estrany, doncs, que aquest paràmetre es consideri el més important a monitorar en línia per a aquest tipus de processos. Tot i que la majoria de productes biotecnològics son produïts mitjançant cultius de cèl·lules animals que creixen en suspensió...

Anticorpos anti-Leptospira spp. em animais selvagens do zoológico municipal de Ribeirão Preto, estado de São Paulo, Brasil; Anti-Leptospira spp. antibodies in wild animals from Ribeirão Preto city zoo in São Paulo State, Brazil

Silva, Carolina dos Santos; Gírio, Raul José Silva; Guerra Neto, Guilherme; Brich, Michelle; Santana, Lucas Alves de Souza; Amâncio, Fábio Henrique; Mariani, Julia Rassi; Wessort, Pedro Meirelles Favaretto
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; ; Formato: application/pdf
Publicado em 01/06/2010 POR
Relevância na Pesquisa
36.17%
A leptospirose acomete todos os animais domésticos, selvagens e os seres humanos. Alguns estudos sorológicos realizados têm demonstrado o envolvimento de espécies selvagens na epidemiologia da doença. Uma vez que populações cativas de animais selvagens são pouco estudadas, principalmente no Brasil, o presente estudo teve como objetivo a detecção de anticorpos anti-Leptospira spp. em animais selvagens de cativeiro e de vida livre do Zoológico Municipal de Ribeirão Preto, estado de São Paulo, Brasil. Durante o período de março a outubro de 2006 foram colhidas 403 amostras de sangue das quais 388 animais (110 répteis, 143 aves, 110 mamíferos e 25 peixes) e 15 amostras de funcionários do zoológico Dentre as 388 amostras de animais, 339 eram animais cativos e 49 eram animais de vida livre capturados pelo uso de armadilhas. Os soros sanguíneos foram analisados por meio da prova de Soroaglutinação Microscópica (SAM) utilizando-se antígenos de 22 sorovares de leptospiras patogênicas e dois sorovares de leptospiras não patogênicas. Foram reagentes para leptospirose 103 (103/388 = 26,5%) amostras, sendo 92 (92/339 = 27,1%) amostras de animais cativos e 11 (11/49 = 22,4%) de animais de vida livre. As 15 amostras de soro humanas foram negativas. Os títulos sorológicos nas amostras reagentes variaram de 40 a 5.120 com predominância dos títulos 40 e 80 e os sorovares Patoc...

Fungal microbiota of the hair coat of laboratory animals

Couto, Manuela Soares; Laboratory of Microbiology – LAMIC, Department of Biology, State University of Ceará, Av. Paranjana, 1700. CEP: 60.740-903, Fortaleza-CE, Brazil.; Pantoja, Lydia Dayanne Maia; Laboratory of Microbiology – LAMIC, Department of B
Fonte: Universidade Federal Fluminense Publicador: Universidade Federal Fluminense
Tipo: info:eu-repo/semantics/article; CMC; Artigo Original; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em 29/08/2013 POR
Relevância na Pesquisa
36.2%
With the refining of handling techniques for laboratory animals kept in animal rooms in which different work rules are followed,it is fundamental to evaluate the microbiota of the raising environment, as well as of the animals in this environment. It isknown that several saprobe fungi are isolated from hair and skin of experimental animals, being potentially pathogenic inspecific situations. The aim of this research was to determine the fungal microbiota on the coats of laboratory animals fromthe Central Animal Room of the University of Ceará. Samples of hair and skin of 355 healthy animals (150 rats, 150 mice, 40guinea pigs and 15 hamsters) were analyzed. Samples were collected by vigorously brushing the back of the animals andthen cultured on Sabouraud’s dextrose and Mycosel agar. Species identification was based on the analysis of the macroscopicappearance of colonies and microscopy features of the fungi. Development of fungi was observed in 63.95% of the animals.The most frequent fungi were: Scopulariopsis brevicaulis (34.7%), Aspergillus sp. (30%) and Penicillium sp. (16.7%). Inconclusion, fungal microbiota on coat of these animals is diversified, and saprobe fungi occurs in a great number of experimentalanimals.