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Arnica: a multivariate analysis of the botany and ethnopharmacology of a medicinal plant complex in the Iberian Peninsula and the Balearic Islands

Obón, Concepcion; Rivera, Diego; Alonso, Verde; Fajardo, José; Valdés, Arturo; Alcaraz, Francisco; Carvalho, Ana Maria
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
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36.08%
Ethnopharmacological relevance Medicinal plant complexes of different species sharing vernacular names, morphological and aromatic characteristics and uses are common in traditional medicine of different cultures. A quantitative methodology as a tool for ethnopharmacological studies is presented for systematically analyzing morphological and therapeutic features shared by several species integrating such complexes. Aim of the study The aim of this paper is to apply a novel methodology to determine whether complexes are homogeneous and species within the complexes are completely interchangeable or not. Moreover, to find out if those species giving the name to the complex are also those which provide a large number of complex descriptors. For this purpose we study the complex of medicinal plant species which share the vernacular name “Árnica” in the Iberian Peninsula and the Balearic Islands, through the systematic recording of past and current local therapeutic uses and administration forms, plant-parts and localities where the different species are used. Being a newly introduced name in the region, “Árnica” offers an interesting field to study the genesis and dynamics of ethnopharmacological categories and medicinal plant complexes. Methods A systematic review of the botanical...

Improving the antioxidant activity of medicinal and aromatic plants by applying electron beam irradiation

Pereira, Eliana; Barreira, João C.M.; Barros, Lillian; Antonio, Amilcar L.; Bento, Albino; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
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36.07%
Irradiation techniques have been applied for decontamination of aromatic and medicinal herbs. Their popularity in the pharmaceutical and food industry requires specific criteria in terms of microbiological safety [1, 2]. In this study the objective was to evaluate the effects of different doses of electron beam (EB) irradiation (0 kGy – control, 1 kGy and 10 kGy) on the antioxidant activity of Aloysia citrodora P., Melissa officinalis L., Melittis melissophyllum L. and Mentha piperita L.. The antioxidant properties of their infusions and methanolic extracts were evaluated through free radicals scavenging activity, reducing power and inhibition of lipid peroxidation in brain homogenates (TBARS assay). By comparing the results obtained from control and irradiated samples, it was evident that EB induced an increase in the ability to scavenge DPPH radicals and in the reducing power, independently of plant species and extract type. Nevertheless, a similar conclusion could not be performed for TBARS assay. In this case, the infusions from EB irradiated samples have also showed a higher effectiveness as lipid peroxidation inhibitors; however, some of the methanolic extracts (particularly those obtained from A. citrodora and M. piperita) did not allow an activity as high as the one observed in control samples. Even so...

Chemical characterization of a dye processing plant effluent - Identification of the mutagenic components

Oliveira, Danielle P.; Carneiro, Patricia A.; Sakagami, Maureen K.; Zanoni, Maria Valnice Boldrin; Umbuzeiro, Gisela A.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 135-142
ENG
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36.11%
This work shows the chemical characterization of a dye processing plant effluent that was contributing to the mutagenicity previously detected in the Cristais river, São Paulo, Brazil, that had an impact on the quality of the related drinking water. The mutagenic dyes Disperse Blue 373, Disperse Orange 37 and Disperse Violet 93, components of a Black Dye Commercial Product (BDCP) frequently used by the facility, were detected by thin layer chromatography (TLC). The blue and orange dyes were quantified by high performance liquid chromatography (HPLC/DAD) in a raw and treated effluent samples and their contribution to the mutagenicity was calculated based on the potency of each dye for the Salmonella YG1041. In the presence of S9 the Disperse Blue 373 accounted for 2.3% of the mutagenic activity of the raw and 71.5% of the treated effluent. In the absence of S9 the Disperse Blue 373 accounted for 1.3% of the mutagenic activity of the raw and 1.5% of the treated effluent. For the Disperse Orange 37, in the presence of S9, it contributed for 0.5% of the mutagenicity of the raw and 6% of the treated effluent. In the absence of S9; 11.5% and 4.4% of the raw and treated effluent mutagenicity, respectively. The contribution of the Disperse Violet 93 was not evaluated because this compound could not be quantified by HPLC/DAD. Mutagenic and/or carcinogenic aromatic amines were also preliminary detected using gas chromatograph/mass spectrometry in both raw and treated and are probably accounting for part of the observed mutagenicity. The effluent treatment applied by the industry does not seem to remove completely the multagenic compounds. The Salmomella/microsome assay coupled with TLC analysis seems to be an important tool to monitor the efficiency of azo dye processing plant effluent treatments. (c) 2006 Elsevier B.V. All rights reserved.

Analysis of aromatic amines in surface waters receiving wastewater from a textile industry by liquid chromatographic with electrochemical detection

Mazzo, T. M.; Saczk, A. A.; Umbuzeiro, G. A.; Zanoni, Maria Valnice Boldrin
Fonte: Taylor & Francis Inc Publicador: Taylor & Francis Inc
Tipo: Artigo de Revista Científica Formato: 2671-2685
ENG
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36.11%
A high performance liquid chromatography ( HPLC) method with electrochemical detection (ED) was developed for the determination of benzidine, 3,3-dimethylbenzidine, o-toluidine and 3,3-dichlorobenzidine in the wastewater of the textile industry. The aromatic amines were eluted on a reversed phase column Shimadzu Shimpack C-18 using acetonitrile + ammonium acetate (1 x 10(-4) mol L-1) at a ratio 46: 54 v/v as mobile phase, pumped at a flow rate of 1.0 mL min(-1). The electrochemical oxidation of the aromatic amines exhibits well-defined peaks at a potential range of +0.45 to +0.78 V on a glassy carbon electrode. Optimum working potentials for amperometric detection were from 0.70 V to +1.0 V vs. Ag/AgCl. Analytical curves for all the aromatic amines studied using the best experimental conditions present linear relationship from 1 x 10(-8) mol L-1 to 1.5 x 10(-5) mol L-1, r = 0.99965, n = 15. Detection limits of 4.5 nM (benzidine), 1.94 nM (o-toluidine), 7.69 nM (3,3-dimethylbenzidine), and 5.15 nM (3,3-dichlorobenzidine) were achieved, respectively. The detection limits were around 10 times lower than that verified for HPLC with ultra violet detection. The applicability of the method was demonstrated by the determination of benzidine in wastewater from the textile industry dealing with an azo dye processing plant.

A convenient method for the determination of moisture in aromatic plants

Pimentel,Flávio A.; Cardoso,Maria das Graças; Salgado,Ana Paula S. P.; Aguiar,Priscila M.; Silva,Vanisse de F.; Morais,Augusto Ramalho de; Nelson,David Lee
Fonte: Sociedade Brasileira de Química Publicador: Sociedade Brasileira de Química
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2006 EN
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46.02%
A method is proposed for the determination of the moisture content of aromatic plants. This method is based on the co-distillation of the starting material in a modified Clevenger apparatus with four organic solvents (toluene, cyclohexane, dichloromethane and carbon tetrachloride). The results were compared with those obtained by oven drying at 105 ºC and steam distillation of the essential oil. The efficiencies of the methods were shown to be equivalent. The solvent distillation method was more practical, especially with respect to operating time (2 h).

Light intensity on growth, leaf micromorphology and essential oil production of Ocimum gratissimum

Fernandes,Valéria Ferreira; Almeida,Laís B. de; Feijó,Emily V. R. da S.; Silva,Delmira da C.; Oliveira,Rosilene A. de; Mielke,Marcelo S.; Costa,Larissa C. do B.
Fonte: Sociedade Brasileira de Farmacognosia Publicador: Sociedade Brasileira de Farmacognosia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2013 EN
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36.15%
Light conditions can promote the growth and development of plants and contribute to increase the essential oil production of commercially cultivated medicinal and aromatic species. In view of the great importance of Ocimum gratissimum L., Lamiaceae, as an aromatic plant, the objective of this work was to determine the effect of light intensities (approximately 4, 7, 11 and 20 mol m-2 d-1) on growth, foliar micromorphology, essential oil content, yield and chemical composition of O. gratissimum. Biomass production of different organs, root:shoot ratio and leaf mass per area were found to linearly increase with increased light availability, whereas stem dry matter fraction, number of leaves, leaf area and plant height have increased up to 10 mol m-2 d-1 and decreased from this value. The tector trichomes density increased with increased light availability, but there was no effect of light treatments on the glandular trichomes density and essential oil content. Regardless of the light level, the major component of the essential oil was eugenol. The essential oil yield per plant increased linearly with light intensity as a direct effect of increased leaf biomass under similar conditions.

Arabidopsis CYP98A3 Mediating Aromatic 3-Hydroxylation. Developmental Regulation of the Gene, and Expression in Yeast1

Nair, Ramesh B.; Xia, Qun; Kartha, Cyril J.; Kurylo, Eugen; Hirji, Rozina N.; Datla, Raju; Selvaraj, Gopalan
Fonte: American Society of Plant Physiologists Publicador: American Society of Plant Physiologists
Tipo: Artigo de Revista Científica
Publicado em /09/2002 EN
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The general phenylpropanoid pathways generate a wide array of aromatic secondary metabolites that range from monolignols, which are ubiquitous in all plants, to sinapine, which is confined to crucifer seeds. The biosynthesis of these compounds involves hydroxylated and methoxylated cinnamyl acid, aldehyde, or alcohol intermediates. Of the three enzymes originally proposed to hydroxylate the 4-, 3-, and 5-positions of the aromatic ring, cinnamate 4-hydroxylase (C4H), which converts trans-cinnamic acid to p-coumaric acid, is the best characterized and is also the archetypal plant P450 monooxygenase. Ferulic acid 5-hydroxylase (F5H), a P450 that catalyzes 5-hydroxylation, has also been studied, but the presumptive 3-hydroxylase converting p-coumarate to caffeate has been elusive. We have found that Arabidopsis CYP98A3, also a P450, could hydroxylate p-coumaric acid to caffeic acid in vivo when expressed in yeast (Saccharomyces cerevisiae) cells, albeit very slowly. CYP98A3 transcript was found in Arabidopsis stem and silique, resembling both C4H and F5H in this respect. CYP98A3 showed further resemblance to C4H in being highly active in root, but differed from F5H in this regard. In transgenic Arabidopsis, the promoters of CYP98A3 and C4H showed wound inducibility and a comparable developmental regulation throughout the life cycle...

Chemotaxis of Azospirillum Species to Aromatic Compounds

Lopez-de-Victoria, Geralyne; Lovell, Charles R.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1993 EN
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Chemotaxis of Azospirillum lipoferum Sp 59b and Azospirillum brasilense Sp 7 and Sp CD to malate and to the aromatic substrates benzoate, protocatechuate, 4-hydroxybenzoate, and catechol was assayed by the capillary method and direct cell counts. A. lipoferum required induction by growth on 4-hydroxybenzoate for positive chemotaxis to this compound. Chemotaxis of Azospirillum spp. to all other substrates did not require induction. Maximum chemotactic responses for most aromatic compounds occurred at concentrations of 1 to 10 mM for A. lipoferum and 100 μM to 1 mM for A. brasilense. Threshold levels of these chemoattractants ranged from nanomolar to micromolar, with A. brasilense Sp CD showing the lowest threshold levels for the substrates tested. Benzoate was the strongest chemoattractant tested, with threshold concentrations in the nanomolar to picomolar range for all strains. Azospirillum spp. clearly have more sensitive chemosensory mechanisms for certain aromatic substrates than previously reported in some other soil bacteria. This sensitivity allows Azospirillum spp. to detect and respond to aromatic substrates at concentrations relevant to the soil and rhizosphere environments. The ability to detect such low concentrations of aromatic compounds in soils may confer advantages in survival and colonization of the rhizosphere by Azospirillum species.

PRT1 of Arabidopsis Is a Ubiquitin Protein Ligase of the Plant N-End Rule Pathway with Specificity for Aromatic Amino-Terminal Residues1

Stary, Susanne; Yin, Xiao-jun; Potuschak, Thomas; Schlögelhofer, Peter; Nizhynska, Victoria; Bachmair, Andreas
Fonte: The American Society for Plant Biologists Publicador: The American Society for Plant Biologists
Tipo: Artigo de Revista Científica
Publicado em /11/2003 EN
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36.25%
The gene PRT1 of Arabidopsis, encoding a 45-kD protein with two RING finger domains, is essential for the degradation of F-dihydrofolate reductase, a model substrate of the N-end rule pathway of protein degradation. We have determined the function of PRT1 by expression in yeast (Saccharomyces cerevisiae). PRT1 can act as a ubiquitin protein ligase in the heterologous host. The identified substrates of PRT1 have an aromatic residue at their amino-terminus, indicating that PRT1 mediates degradation of N-end rule substrates with aromatic termini but not of those with aliphatic or basic amino-termini. Expression of model substrates in mutant and wild-type plants confirmed this substrate specificity. A ligase activity exclusively devoted to aromatic amino-termini of the N-end rule pathway is apparently unique to plants. The results presented also imply that other known substrates of the plant N-end rule pathway are ubiquitylated by one or more different ubiquitin protein ligases.

Glycosylphosphatidylinositol Lipid Anchoring of Plant Proteins. Sensitive Prediction from Sequence- and Genome-Wide Studies for Arabidopsis and Rice1

Eisenhaber, Birgit; Wildpaner, Michael; Schultz, Carolyn J.; Borner, Georg H.H.; Dupree, Paul; Eisenhaber, Frank
Fonte: The American Society for Plant Biologists Publicador: The American Society for Plant Biologists
Tipo: Artigo de Revista Científica
Publicado em /12/2003 EN
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36.07%
Posttranslational glycosylphosphatidylinositol (GPI) lipid anchoring is common not only for animal and fungal but also for plant proteins. The attachment of the GPI moiety to the carboxyl-terminus after proteolytic cleavage of a C-terminal propeptide is performed by the transamidase complex. Its four known subunits also have obvious full-length orthologs in the Arabidopsis and rice (Oryza sativa) genomes; thus, the mechanism of substrate protein processing appears similar for all eukaryotes. A learning set of plant proteins (substrates for the transamidase complex) has been collected both from the literature and plant sequence databases. We find that the plant GPI lipid anchor motif differs in minor aspects from the animal signal (e.g. the plant hydrophobic tail region can contain a higher fraction of aromatic residues). We have developed the “big-Π plant” program for prediction of compatibility of query protein C-termini with the plant GPI lipid anchor motif requirements. Validation tests show that the sensitivity for transamidase targets is approximately 94%, and the rate of false positive prediction is about 0.1%. Thus, the big-Π predictor can be applied as unsupervised genome annotation and target selection tool. The program is also suited for the design of modified protein constructs to test their GPI lipid anchoring capacity. The big-Π plant predictor Web server and lists of potential plant precursor proteins in Swiss-Prot...

Homology Modeling of Representative Subfamilies of Arabidopsis Major Intrinsic Proteins. Classification Based on the Aromatic/Arginine Selectivity Filter1[w]

Wallace, Ian S.; Roberts, Daniel M.
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Artigo de Revista Científica
Publicado em /06/2004 EN
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36.11%
Major intrinsic proteins (MIPs) are a family of membrane channels that facilitate the bidirectional transport of water and small uncharged solutes such as glycerol. The 35 full-length members of the MIP family in Arabidopsis are segregated into four structurally homologous subfamilies: plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin 26-like intrinsic membrane proteins (NIPs), and small basic intrinsic proteins (SIPs). Computational methods were used to construct structural models of the putative pore regions of various plant MIPs based on homology modeling with the atomic resolution crystal structures of mammalian aquaporin 1 and the bacterial glycerol permease GlpF. Based on comparisons of the narrow selectivity filter regions (the aromatic/Arg [ar/R] filter), the members of the four phylogenetic subfamilies of Arabidopsis MIPs can be classified into eight groups. PIPs possess a uniform ar/R signature characteristic of high water transport aquaporins, whereas TIPs are highly diverse with three separate conserved ar/R regions. NIPs possess two separate conserved ar/R regions, one that is similar to the archetype, soybean (Glycine max) nodulin 26, and another that is characteristic of Arabidopsis NIP6;1. The SIP subfamily possesses two ar/R subgroups...

AraPerox. A Database of Putative Arabidopsis Proteins from Plant Peroxisomes1[w]

Reumann, Sigrun; Ma, Changle; Lemke, Steffen; Babujee, Lavanya
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Artigo de Revista Científica
Publicado em /09/2004 EN
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36.05%
To identify unknown proteins from plant peroxisomes, the Arabidopsis genome was screened for proteins with putative major or minor peroxisome targeting signals type 1 or 2 (PTS1 or PTS2), as defined previously (Reumann S [2004] Plant Physiol 135: 783–800). About 220 and 60 proteins were identified that carry a putative PTS1 or PTS2, respectively. To further support postulated targeting to peroxisomes, several prediction programs were applied and the putative targeting domains analyzed for properties conserved in peroxisomal proteins and for PTS conservation in homologous plant expressed sequence tags. The majority of proteins with a major PTS and medium to high overall probability of peroxisomal targeting represent novel nonhypothetical proteins and include several enzymes involved in β-oxidation of unsaturated fatty acids and branched amino acids, and 2-hydroxy acid oxidases with a predicted function in fatty acid α-oxidation, as well as NADP-dependent dehydrogenases and reductases. In addition, large protein families with many putative peroxisomal isoforms were recognized, including acyl-activating enzymes, GDSL lipases, and small thioesterases. Several proteins are homologous to prokaryotic enzymes of a novel aerobic hybrid degradation pathway for aromatic compounds and proposed to be involved in peroxisomal biosynthesis of plant hormones like jasmonic acid...

A Rapid, High Resolution High Performance Liquid Chromatography Profiling Procedure for Plant and Microbial Aromatic Secondary Metabolites 1

Graham, Terrence L.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1991 EN
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36.11%
High performance liquid chromatography protocols have been developed to allow the simultaneous analysis of a very wide range of soluble aromatic secondary metabolites in unfractionated biological extracts. The methods are simple, sensitive, and highly reproducible. They are applicable to a wide variety of natural product investigations in both plants and microorganisms. High resolution of metabolites is achieved in 25 minutes by chromatography on a reverse phase C18 column in a gradient of 0 to 55% acetonitrile in water at pH 3. For example, near-baseline resolution of over 20 phenylpropanoid metabolites and 18 naturally occurring metabolites of indole-3-acetic acid can be obtained. The methods can be applied directly to whole tissue extracts without prepurification or enrichment. Moreover, the simplicity and sensitivity of the protocols allow their application to a large number of very small tissue samples, such as those encountered in research on host-microbe interactions. Such profiles allow one to monitor simultaneously the various alternative metabolic fates of a complex array of molecules. Examination of the profiles over time thus provides one with a powerful tool to correlate many concurrent molecular events that may relate to a given biological phenomenon. The final protocol requires as little as 1 milligram of tissue...

Decomposers and root feeders interactively affect plant defence in Sinapis alba

Lohmann, Maité; Scheu, Stefan; Müller, Caroline
Fonte: Springer-Verlag Publicador: Springer-Verlag
Tipo: Artigo de Revista Científica
EN
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36.23%
Aboveground herbivory is well known to change plant growth and defence. In contrast, effects of soil organisms, acting alone or in concert, on allocation patterns are less well understood. We investigated separate and combined effects of the endogeic earthworm species Aporrectodea caliginosa and the root feeding nematode species Pratylenchus penetrans and Meloidogyne incognita on plant responses including growth and defence metabolite concentrations in leaves of white mustard, Sinapis alba. Soil biota had a strong impact on plant traits, with the intensity varying due to species combinations. Nematode infestation reduced shoot biomass and nitrogen concentration but only in the absence of earthworms. Earthworms likely counteracted the negative effects of nematodes. Infestation with the migratory lesion-nematode P. penetrans combined with earthworms led to increased root length. Earthworm biomass increased in the presence of this species, indicating that these nematodes increased the food resources of earthworms—presumably dead and decaying roots. Nitrogen-based defence compounds, i.e. glucosinolates, did not correlate with nitrogen levels. In the presence of earthworms, concentrations of aromatic glucosinolates in leaves were significantly increased. In contrast...

The Biosynthetic Pathways for Shikimate and Aromatic Amino Acids in Arabidopsis thaliana

Tzin, Vered; Galili, Gad
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Artigo de Revista Científica
Publicado em 17/05/2010 EN
Relevância na Pesquisa
36.29%
The aromatic amino acids phenylalanine, tyrosine and tryptophan in plants are not only essential components of protein synthesis, but also serve as precursors for a wide range of secondary metabolites that are important for plant growth as well as for human nutrition and health. The aromatic amino acids are synthesized via the shikimate pathway followed by the branched aromatic amino acid metabolic pathway, with chorismate serving as a major branch point intermediate metabolite. Yet, the regulation of their synthesis is still far from being understood. So far, only three enzymes in this pathway, namely, chorismate mutase of phenylalanine and tyrosine synthesis, tryptophan synthase of tryptophan biosynthesis and arogenate dehydratase of phenylalanine biosynthesis, proved experimentally to be allosterically regulated. The major biosynthesis route of phenylalanine in plants occurs via arogenate. Yet, recent studies suggest that an alternative route of phynylalanine biosynthesis via phenylpyruvate may also exist in plants, similarly to many microorganisms. Several transcription factors regulating the expression of genes encoding enzymes of both the shikimate pathway and aromatic amino acid metabolism have also been recently identified in Arabidopsis and other plant species.

Flavonoid Biosynthesis Genes Putatively Identified in the Aromatic Plant Polygonum minus via Expressed Sequences Tag (EST) Analysis

Roslan, Nur Diyana; Yusop, Jastina Mat; Baharum, Syarul Nataqain; Othman, Roohaida; Mohamed-Hussein, Zeti-Azura; Ismail, Ismanizan; Noor, Normah Mohd; Zainal, Zamri
Fonte: Molecular Diversity Preservation International (MDPI) Publicador: Molecular Diversity Preservation International (MDPI)
Tipo: Artigo de Revista Científica
Publicado em 28/02/2012 EN
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46.05%
P. minus is an aromatic plant, the leaf of which is widely used as a food additive and in the perfume industry. The leaf also accumulates secondary metabolites that act as active ingredients such as flavonoid. Due to limited genomic and transcriptomic data, the biosynthetic pathway of flavonoids is currently unclear. Identification of candidate genes involved in the flavonoid biosynthetic pathway will significantly contribute to understanding the biosynthesis of active compounds. We have constructed a standard cDNA library from P. minus leaves, and two normalized full-length enriched cDNA libraries were constructed from stem and root organs in order to create a gene resource for the biosynthesis of secondary metabolites, especially flavonoid biosynthesis. Thus, large-scale sequencing of P. minus cDNA libraries identified 4196 expressed sequences tags (ESTs) which were deposited in dbEST in the National Center of Biotechnology Information (NCBI). From the three constructed cDNA libraries, 11 ESTs encoding seven genes were mapped to the flavonoid biosynthetic pathway. Finally, three flavonoid biosynthetic pathway-related ESTs chalcone synthase, CHS (JG745304), flavonol synthase, FLS (JG705819) and leucoanthocyanidin dioxygenase, LDOX (JG745247) were selected for further examination by quantitative RT-PCR (qRT-PCR) in different P. minus organs. Expression was detected in leaf...

Biochemical Evaluation of the Decarboxylation and Decarboxylation-Deamination Activities of Plant Aromatic Amino Acid Decarboxylases*

Torrens-Spence, Michael P.; Liu, Pingyang; Ding, Haizhen; Harich, Kim; Gillaspy, Glenda; Li, Jianyong
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: Artigo de Revista Científica
EN
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36.11%
Background: Plant arylalkylamine and aldehyde synthesizing aromatic amino acid decarboxylases (AAAD) are effectively indistinguishable.

Invisible light, visible results: gamma irradiation effects on aromatic, edible and medicinal plants

Antonio, Amilcar L.; Pereira, Eliana; Pinela, José; Pereira, Carla; Cabo Verde, Sandra; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
46.4%
Aromatic, edible and medicinal plants require effective conservation technologies to expand their use. During the processing and storage, they can be easily exposed to contamination that can lead to a microbial deterioration or insect infestation compromising its quality and shelf life. In this study, one of the most promising decontamination methods for many foodstuffs and plant materials was applied. The effects of gamma irradiation in chemical (aromatic plant- Aloysia citrodora Palàu), nutritional (edible plant- Nasturtium officinale W.T. Aiton) and anti-hepatocellular carcinoma (medicinal plant- Cochlospermum angolensis Welw.) properties were evaluated for doses up to 10 kGy. The results showed that each plant was differentially affected by irradiation treatment. For aromatic and edible plants, the effects of gamma irradiation in chemical and nutritional properties proved to have statistical significance in some particular cases. However, when analyzed under an integrated approach, non-irradiated and irradiated samples were grouped indiscriminately, indicating that irradiation treatment did not cause sufficient changes to define a specific chemical profile. Regarding the medicinal plant, irradiated samples kept the anti-hepatocellular carcinoma activity...

Herbal infusions for medicinal purposes: comparative study of the nutritional and energetic contribution of twenty-seven aromatic plants

Pereira, Carla; Barros, Lillian; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
36.14%
Aromatic plants have been used worldwide for centuhes for nutritional and medicinal purposes. They are traditionally used as herbal infusions for their attractive aroma and specific taste but also in folk medicine as carminative agents against bronchitis and ulcers, as diuretics, depuratives and vermifuges, as also for their antiscorbutic, antispasmodic, tonic, antimicrobial, anti-inflammatory, antimutagenic and anti-carcinogenic properties [1, 2]. Nevertheless, despite the increasing recognition of their beneficial effects, there is a growing loss of diversity of these herbs [3]. Furthermore, the majority of the studies with plants consumed as infusions that are available in literature do not include analyses in the infusion (which is the real consumed form), but in dry material. Thus, in the present work, we aimed to provide scientific information concerning the nutritional value and energetic contribution of infusions prepared from twenty-seven widely used plant species in order to systematize the results obtained for an easier comparison. Most of the herbal infusions analysed presented fructose, glucose and sucrose in very low amounts, with concentrations ranging from 6. 15 to 26. 80 mg/100 mL, and eight of these infusions did not reveal the presence of any carbohydrate. Chamaespartium tridentatum (L.) P. E. Gibbs. subsp. cantabrícum (Spach) and Equisetum giganteum L. revealed the highest content of fructose (13.60 mg/100 mL) and glucose (12. 65 mg/100 mL)...

Síntesis de análogos de salpichrólidos con anillo D aromático y determinación de su actividad antiestrogénica y antifúngica; Synthesis, antiestrogenic and antifugal activities of salpichrolides analogues with an aromatic D ring

Sonego, Juan Manuel
Fonte: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires Publicador: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
Tipo: info:eu-repo/semantics/doctoralThesis; tesis doctoral; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2012 SPA
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Los withanólidos son un grupo de esteroides naturales de 28 carbonos aislados de plantas pertenecientes al género Solanacea. En particular un pequeño grupo de withanólidos con un anillo D aromático de seis miembros se aisló de la planta Salpichroa origanifolia. Estos compuestos presentan actividad antialimentaria y antiproliferativa. Para determinar la relevancia del anillo D aromático en las propiedades biológicas de los salpichrólidos, en este trabajo de Tesis se sintetizaron ocho esteroides con anillo D aromático, análogos de los salpichrólidos con cadena lateral simplificada. Se describe una nueva metodología para la preparación de esteroides con anillo D aromático, la cual involucra la abstracción de HBr a partir de un homoesteroide dibromado por tratamiento con DABCO. Todos los nuevos compuestos fueron caracterizados usando técnicas de RMN 2D y espectrometría de masas. Se determinaron las actividades antiestrogénica y antifúngica de los análogos sintéticos y de tres salpichrólidos naturales.; Withanolides are a group of naturally occurring C-28 steroids isolated from several genera of Solanacea. In particular a small group of withanolides with a six membered aromatic D ring, the salpichrolides, were isolated from the plant Salpichroa organfiolia. These compounds exhibit antifeedant and antiproliferative activities. To determine the relevance of the aromatic D ring in the biological properties of salpichrolides...