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MEM 004

Curso de Cinema, UFSC
Fonte: Universidade Federal de Santa Catarina Publicador: Universidade Federal de Santa Catarina
PT_BR
Relevância na Pesquisa
26.33%

A comparison of the effects of SCA40, NS 004 and NS 1619 on large conductance Ca(2+)-activated K+ channels in bovine tracheal smooth muscle cells in culture.

Macmillan, S.; Sheridan, R. D.; Chilvers, E. R.; Patmore, L.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1995 EN
Relevância na Pesquisa
26.64%
1. The effects of imidazopyrazine derivative, SCA40, on the activity of single large conductance, Ca(2+)-activated K+ (BKCa) channels in inside-out and outside-out patches from bovine tracheal smooth muscle (BTSM) cells in culture have been compared with those of two established BKCa channel openers, NS 004 and NS 1619. 2. The presence of BKCa channels on inside-out patches of BTSM membranes was confirmed by the single channel conductance (240 pS), selectivity for K+, dependence of channel activity on [Ca2+]i, and sensitivity to the selective BKCa channel blocker, iberiotoxin. 3. NS 004 and ND 1619 (3-30 microM) induced concentration-related increases in open state probability of BKCa channels when applied to either inside-out or outside-out BTSM patches, thus confirming that these compounds are activators of the BKCa channel in this preparation. 4. SCA40 (0.1-10 microM) had no effect on the activity of BKCa channels when applied to either inside-out or outside-out patches which subsequently responded to the application of NS 004 (10-20 microM). 5. It is concluded that SCA40 does not have a direct effect on BKCa channel activity in BTSM patches and that the previously reported relaxant action of SCA40 on tracheal smooth muscle is unlikely to be mediated by this mechanism.

Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention

Taner, Sabrina B.; Pando, Marcelo J.; Roberts, Allison; Schellekens, Jennifer; Marsh, Steven G. E.; Malmberg, Karl-Johan; Parham, Peter; Brodsky, Frances M.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
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Variable interaction between the Bw4 epitope of HLA-B and the polymorphic KIR3DL1/S1 system of inhibitory and activating NK cell receptors diversifies the development, repertoire formation and response of human NK cells. KIR3DL1*004, a common KIR3DL1 allotype, in combination with Bw4+, HLA-B slows progression of HIV infection to AIDS. Analysis here of KIR3DL1*004 membrane traffic in NK cells shows this allotype is largely misfolded but stably retained in the endoplasmic reticulum, where it binds to the chaperone calreticulin and does not induce the unfolded protein response. A small fraction of KIR3DL1*004 folds correctly and leaves the endoplasmic reticulum to be expressed on the surface of primary NK and transfected NKL cells, in a form that can be triggered to inhibit NK cell activation and secretion of interferon-γ. Consistent with this small proportion of correctly-folded molecules, trace amounts of MHC Class I co-immunoprecipitated with KIR3DL1*004. There was no indication of any extensive intracellular interaction between unfolded KIR3DL1*004 and cognate Bw4+ HLA-B. A similarly limited interaction of Bw4 with KIR3DL1*002, when both were expressed by the same cell, was observed despite the efficient folding of KIR3DL1*002 and its abundance on the NK cell surface. Several positions of polymorphism modulate KIR3DL1 abundance at the cell surface...

One Percent Tenofovir Applied Topically to Humanized BLT Mice and Used According to the CAPRISA 004 Experimental Design Demonstrates Partial Protection from Vaginal HIV Infection, Validating the BLT Model for Evaluation of New Microbicide Candidates▿

Denton, Paul W.; Othieno, Florence; Martinez-Torres, Francisco; Zou, Wei; Krisko, John F.; Fleming, Elisa; Zein, Sima; Powell, Daniel A.; Wahl, Angela; Kwak, Youn Tae; Welch, Brett D.; Kay, Michael S.; Payne, Deborah A.; Gallay, Philippe; Appella, Ettore;
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /08/2011 EN
Relevância na Pesquisa
26.52%
Recent iPrEx clinical trial results provided evidence that systemic preexposure prophylaxis (PrEP) with emtricitabine (FTC) and tenofovir disoproxil fumarate (TDF) can partially prevent rectal HIV transmission in humans. Similarly, we have previously demonstrated that systemic administration of the same FTC-TDF combination efficiently prevented rectal transmission in humanized bone marrow/liver/thymus (BLT) mice. The CAPRISA 004 trial recently demonstrated that topical application of the tenofovir could partially prevent vaginal HIV-1 transmission in humans. To further validate the usefulness of the BLT mouse model for testing HIV prevention strategies, we evaluated the topical administration of tenofovir as used in CAPRISA 004 to prevent vaginal HIV transmission in BLT mice. Our results demonstrate that vaginally administered 1% tenofovir significantly reduced HIV transmission in BLT mice (P = 0.002). Together with the results obtained after systemic antiretroviral PrEP, these topical inhibitor data serve to validate the use of humanized BLT mice to evaluate both systemic and topical inhibitors of HIV transmission. Based on these observations, we tested six additional microbicide candidates for their ability to prevent vaginal HIV transmission: a C-peptide fusion inhibitor (C52L)...

Antioxidant effects of D-004, a lipid extract from the Roystonea regia fruit, on the plasma of healthy men

López, Ernesto; Molina, Vivian; Illnait, José; Oyarzábal, Ambar; Fernández, Lilia C.; Más, Rosa; Gámez, Rafael; Fernández, Julio C.; Jiménez, Sonia; Mesa, Meilis; Hollands, Ivón; Mendoza, Sarahí
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
26.82%
The aim of this study was to conduct a randomized, double-blind and placebo-controlled study to investigate the effects of D-004, a lipid extract of the Roystonea regia fruit that prevents testosterone- and phenylepinephrine-induced prostate hyperplasia in rodents, on plasma oxidative markers in healthy men. We enrolled male volunteers (20–55 years) in good health and without lower urinary tract symptoms. Thirty-four eligible participants were randomized to placebo or D-004 (320 mg) capsules administered daily for 6 weeks. An interim check-up and a final visit were conducted after 3 and 6 weeks of therapy, respectively. Physical examinations were performed at each visit, and laboratory tests were performed at baseline and at treatment completion. Oxidative variables included plasma malondialdehyde (MDA), total hydroxyperoxides (TOH), sulphydryl (SH) groups and total antioxidant status (TAS). We assessed treatment compliance and addressed adverse experiences (AEs) at weeks 3 and 6. At week 6, with D-004, the mean reductions of plasma MDA (26.7%), TOH (18.8%) and SH groups (31.6%), and the mean increase of TAS (35.3%) were significantly different from those of placebo (P < 0.001 for plasma TAS, P < 0.0001 for all other comparisons). D-004 did not differ from the placebo in safety indicators. There were two withdrawals (both in the D-004 group)...

Assessing Adherence in the CAPRISA 004 Tenofovir Gel HIV Prevention Trial: Results of a Nested Case–Control Study

MacQueen, Kathleen M.; Weaver, Mark A.; van Loggerenberg, Francois; Succop, Stacey; Majola, Nelisle; Taylor, Doug; Karim, Quarraisha Abdool; Karim, Salim Abdool
Fonte: Springer US Publicador: Springer US
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
26.52%
Adherence undeniably impacts product effectiveness in microbicide trials, but the connection has proven challenging to quantify using routinely collected behavioral data. We explored this relationship using a nested case–control study in the CAPRISA 004 Tenofovir (TFV) gel HIV prevention trial. Detailed 3-month recall data on sex events, condom and gel use were collected from 72 incident cases and 205 uninfected controls. We then assessed how the relationship between self-reported adherence and HIV acquisition differed between the TFV and placebo gel groups, an interaction effect that should exist if effectiveness increases with adherence. The CAPRISA 004 trial determined that randomization to TFV gel was associated with a significant reduction in risk of HIV acquisition. In our nested case–control study, however, we did not observe a meaningful decrease in the relative odds of infection—TFV versus placebo—as self-reported adherence increased. To the contrary, exploratory sub-group analysis of the case–control data identified greater evidence for a protective effect of TFV gel among participants reporting less than 80 % adherence to the protocol-defined regimen (odds ratio (OR) 0.30; 95 % CI 0.11–0.78) than among those reporting ≥80 % adherence (Odds Ratio 0.81; 95 % CI 0.34–1.92). The small number of cases may have inhibited our ability to detect the hypothesized interaction between adherence and effectiveness. Nonetheless...

Adherence in the CAPRISA 004 Tenofovir Gel Microbicide Trial

Mansoor, Leila E
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/2014 EN
Relevância na Pesquisa
26.52%
High adherence is key to microbicide effectiveness. Here we provide a description of adherence interventions and the adherence rates achieved in the CAPRISA 004 Tenofovir Gel Trial. Adherence support for the before-and-after dosing strategy (BAT 24) was provided at enrolment and at each monthly study visit. This initially comprised individual counselling and was replaced midway by a structured theory-based adherence support program (ASP) based on motivational interviewing. The 889 women were followed for an average of 18 months and attended a total of 17031 monthly visits. On average women reported 5 sex acts and returned 5.9 empty applicators per month. The adherence rate based on applicator count in relation to all reported sex acts was 72.2% compared to the 82.0% self-reported adherence during the last sex act. Adherence support activities, which achieve levels of adherence similar to or better than those achieved by the CAPRISA 004 ASP, will be critical to the success of future microbicide trials.

Sensitive Tenofovir Resistance Screening of HIV-1 From the Genital and Blood Compartments of Women With Breakthrough Infections in the CAPRISA 004 Tenofovir Gel Trial

Wei, Xierong; Hunt, Gillian; Abdool Karim, Salim S.; Naranbhai, Vivek; Sibeko, Sengeziwe; Abdool Karim, Quarraisha; Li, Jin-fen; Kashuba, Angela D. M.; Werner, Lise; Passmore, Jo-Ann S.; Morris, Lynn; Heneine, Walid; Johnson, Jeffrey A.
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
26.52%
The Centre for the AIDS Programme of Research in South Africa 004 (CAPRISA 004) study demonstrated that vaginally applied tenofovir gel is a promising intervention for protecting women from sexually acquiring human immunodeficiency virus (HIV). However, the potential for emergence of tenofovir resistance remains a concern in women who seroconvert while using the gel despite the lack of plasma virus resistance as assessed by population sequencing during the trial. We applied highly sensitive polymerase chain reaction–based assays to screen for tenofovir resistance in plasma and vaginal swab specimens. The absence of mutation detection suggested little immediate risk of tenofovir-resistant HIV-1 emergence and forward transmission in settings in which gel users are closely monitored for HIV seroconversion.

Design and production of a prototype wheeled pendulum for the new 2.004 laboratory

Yoon, Jane Sujin
Fonte: Massachusetts Institute of Technology Publicador: Massachusetts Institute of Technology
Tipo: Tese de Doutorado Formato: 45 leaves
ENG
Relevância na Pesquisa
26.52%
The goal of this thesis was to create a piece of physical hardware that would be suited for a multi-week design project for the design component of the Dynamics and Control II (M.I.T. course number: 2.004) laboratory. The wheeled pendulum model was chosen as an appropriate system to use in the second half of the laboratory component of 2.004 because of its clearly defined and well understood system. The inverted (or wheeled) pendulum is a classic controls problem-in the absence of a controls component, the system is non-linear and unstable. This thesis analyzes the system dynamics by deriving the equations of motion for the wheeled pendulum, and uses mathematical modeling (MatlabTM) to further understand the instability of the system. Based on the Matlab models, several design iterations were developed, and a robust, functional prototype of the wheeled pendulum was created.; by Jane Sujin Yoon.; Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006.; Includes bibliographical references (leaf 32).

Los órganos de la quiebra : con las modificaciones introducidas por la ley no. 20.004 y por la ley no. 20.073

Carrasco Delgado, Nicolás Ignacio
Fonte: Universidad de Chile Publicador: Universidad de Chile
Tipo: Tesis
ES
Relevância na Pesquisa
36.64%
Memoria (licenciado en ciencias jurídicas y sociales); La preocupación primera que surge sobre el derecho orgánico de la quiebra dice relación con el poco desarrollo doctrinario a nivel nacional que al respecto existe. En efecto, sólo un libro se ha dedicado completamente al tema, que es el libro del profesor Oscar Torres, titulado: “Los órganos de la quiebra”. A ello, debemos sumar algunas tesis que se refieren a materias vinculadas a los órganos de la quiebra, pero que no lo tratan en su generalidad, sino que parcialmente. Este escenario no nos debe sorprender, y más aún en Chile, en donde el desarrollo dogmático del derecho ha quedado atrás en relación con lo que ocurre en el derecho comparado. En definitiva debemos concluir que el desarrollo doctrinal en materia de derecho mercantil es escaso, experiencia que se comparte en especial en el derecho concursal. Constatando lo anterior, pretendemos humildemente contribuir al desarrollo de este aspecto del derecho concursal, referido como ya lo hemos indicado, al derecho orgánico de la quiebra. El primer objetivo, es en consecuencia, incorporar raciocinio jurídico a una discusión académica que se encuentra dormida. Asimismo, surge una segunda problemática...

A separação de irmãos no caso de colocação de criança em família substituta para guarda e adoção, conforme o ECA e artigo 28 §4º da lei 12010/2009: um estudo de caso referente aos autos 004.09.004020-5 e 004.12.009206-2, que tramitaram na 3˚ vara cível na comarca de Araranguá

Borges, Cândida de Moraes
Fonte: Universidade do Extremo Sul Catarinense Publicador: Universidade do Extremo Sul Catarinense
Tipo: Trabalho de Conclusão de Curso - TCC
PT_BR
Relevância na Pesquisa
26.64%
Trabalho de Conclusão de Curso, apresentado para obtenção do grau de Bacharel no Curso de Direito da Universidade do Extremo Sul Catarinense, UNESC.; De acordo com o artigo 28 §4˚ do Estatuto da Criança e do Adolescente, os irmãos serão colocados em mesma família substituta sob guarda, tutela e adoção. O presente trabalho tem como problema de pesquisa a questão da violação do dispositivo mencionado. Seu objetivo geral é compreender os critérios judiciais para a adoção e guarda, quando há separação de irmãos, sob ótica do mesmo Estatuto. Apresentará especialmente os princípios do melhor interesse da criança e do adolescente e o princípio da proteção integral. Analisará a possibilidade da colocação de apenas um dos irmãos em família substituta, bem como a possibilidade da separação de irmãos em família substituta, em confronto com os princípios mencionados. A primeira hipótese apresentada seria quanto à violação do dispositivo citado em caso de separação de irmãos. De outro lado a colocação de apenas um dos irmãos em família substituta, causando a separação de irmãos pode tornar-se exequível se considerados os princípios do melhor interesse da criança e do adolescente, princípio da prioridade absoluta e a doutrina da proteção integral. Este trabalho foi dividido em três capítulos...

VLT/X-shooter observations of blue compact galaxies Haro 11 and ESO 338-IG 004

Guseva, N. G.; Izotov, Y. I.; Fricke, K. J.; Henkel, C.
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 23/03/2012
Relevância na Pesquisa
26.52%
(abridged) Strongly star-forming galaxies of subsolar metallicities are typical of the high-redshift universe. Here we therefore provide accurate data for two low-z analogs, the well-known low-metallicity emission-line galaxies Haro 11 and ESO 338-IG 004. On the basis of Very Large Telescope/X-shooter spectroscopic observations in the wavelength range 3000-24000\AA, we use standard direct methods to derive physical conditions and element abundances. Furthermore, we use X-shooter data together with Spitzer observations in the mid-infrared range to attempt to find hidden star formation. We derive interstellar oxygen abundances of 12 + log O/H = 8.33+/-0.01, 8.10+/-0.04, and 7.89+/-0.01 in the two HII regions B and C of Haro 11 and in ESO 338-IG 004, respectively. The observed fluxes of the hydrogen lines correspond to the theoretical recombination values after correction for extinction with a single value of the extinction coefficient C(Hbeta) across the entire wavelength range from the near-ultraviolet to the NIR and mid-infrared for each of the studied HII regions. Therefore there are no emission-line regions contributing to the line emission in the NIR range, which are hidden in the optical range. The agreement between the extinction-corrected and CLOUDY-predicted fluxes implies that a HII region model including only stellar photoionisation is able to account for the observed fluxes...

The stellar velocity dispersion of the lens galaxy in MG2016+112 at z=1.004

Koopmans, L. V. E.; Treu, T.
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
26.52%
We present a direct measurement of the stellar velocity dispersion of the early-type lens galaxy D in the system MG2016+112 (z=1.004), determined from a spectrum obtained with the Echelle Spectrograph and Imager (ESI) on the W.M. Keck-II Telescope, as part of the Lenses Structure and Dynamics (LSD) Survey. We find a velocity dispersion of sigma_ap=304+-27 km/s inside an effective circular aperture with a radius of 0.65'', corresponding to a central velocity dispersion of sigma=328+-32 km/s. From a Hubble Space Telescope F160W-band image, we measure the effective radius and effective surface brightness in order to determine the offset of the lens galaxy with respect to the local Fundamental Plane. The offset corresponds to an evolution of the rest-frame effective mass-to-light ratio of Delta log(M/L_B)=-0.62+-0.08 from z=0 to z=1.004. By interpreting colors and offset of the FP with two independent stellar population synthesis models, we obtain a single-burst equivalent age of 2.8+-0.8 Gyr (i.e. z_f>1.9) and a supersolar metallicity of log[Z/Z_sun]=0.25+-0.25. The lens galaxy is therefore a massive elliptical dominated by an old and metal rich stellar population at z>1. The excellent agreement of the stellar velocity dispersion with that predicted from recent lens models confirms that the angular separation of the multiple images of the background QSO is predominantly due to the lens galaxy...

Decreto n. 2.004, 13 fev. 1911, SP.

Lins, Manoel Joaquim Albuquerque
Fonte: Universidade Federal de Santa Catarina Publicador: Universidade Federal de Santa Catarina
Tipo: Documento
PT_BR
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26.52%
O decreto estabelece o programa das escolas modelos isoladas, anexas às Escolas Normais do estado, com discriminação dos seguintes elementos dos saberes matemáticos: números, tornos, cópia de Praker. SÃO PAULO, Decreto n. 2.004, de 13 de fevereiro de 1911. Aprova o regimento interno das escolas modelos isoladas, anexas à Escola Normal de São Paulo, 1911. Disponível em: http://www.al.sp.gov.br/repositorio/legislacao/decreto/1911/decreto-2004-13.02.1911.html Acesso em: 26. out. 2015; Aprova o Regimento Interno das Escolas Modelos Isoladas, anexas á Escola Normal de São Paulo. Trecho do decreto n. 2.004, de 13 de fevereiro de 1911, aprovado pelo presidente do estado, Manoel Joaquim Albuquerque Lins

Evaluación genotóxica del D-004, extracto del fruto de Roystonea regia, mediante el ensayo de micronúcleos

Arencibia Arrebola,Daniel Francisco; Gutiérrez Martínez,Ariadne; Gámez Menéndez,Rafael; Pardo Acosta,Balia; Curveco Sánchez,Dayisell; García Cambián,Haydeé
Fonte: Revista Cubana de Farmacia Publicador: Revista Cubana de Farmacia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2009 ES
Relevância na Pesquisa
36.77%
El D-004 es un extracto lipídico del fruto de la palma real (Roystonea regia), que ha demostrado ser eficaz en prevenir la hiperplasia prostática inducida por testosterona y por fenilefrina en modelos experimentales en ratas. El objetivo fue determinar si el D-004 induce cambios en la frecuencia de aparición de micronúcleos al realizar la administración oral a dosis repetida durante 8 semanas. Se formaron 5 grupos experimentales (7 animales/grupo): un grupo control solvente, tres tratados con D-004 (500, 1 000 y 1 500 mg/kg) y un control positivo tratado con ciclofosfamida. No ocurrieron muertes ni se detectaron signos clínicos de toxicidad. No hubo diferencias significativas entre controles y tratados en cuanto a la frecuencia de eritrocitos policromatófilos micronucleados y el índice de citotoxicidad. En conclusión, el D-004 administrado por vía oral a las dosis empleadas no presenta actividad clastogénica ni citotóxica en médula ósea de ratones OF-1 machos in vivo.

Effect of D-004, a lipid extract from royal palm (Roystonea regia) fruits, on phenylephrine-induced contractions of isolated rat prostate

Arruzazabala Valmaña,Lourdes; Más Ferreiro,Rosa; Pérez Guerra,Yohani; Ravelo Calzado,Yazmin; Molina Cuevas,Vivian; Carbajal Quintana,Daisy
Fonte: Revista Cubana de Farmacia Publicador: Revista Cubana de Farmacia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2009 ES
Relevância na Pesquisa
36.85%
D-004, un extracto lipídico de las frutas de Roystonea regia, inhibe la contracción provocada por la norepinefrina en el vas deferens de las ratas. No obstante, el efecto de D-004 sobre las respuestas contráctiles adrenérgicas a1 en las preparaciones aisladas de la próstata no ha sido estudiado. Por lo tanto, este estudio investigó los efectos de D-004 sobre las contracciones provocadas por la fenilefrina en las extracciones aisladas de la próstata de la rata. D-004 (125-500 mg/mL) fue adicionado a las preparaciones a partir de la próstata en la que las contracciones fueron provocadas por la fenilefrina (10-6-10-4 M) o por CIK (50 mM). D-004 (250-500 mg/mL) en forma significativa y la dosis en forma dependiente inhibieron las contracciones provocadas por la fenilefrina mediante un mecanismo no competetitivo, reduciendo así (12-60 %) la contracción máxima por la fenilefrina con respecto a las preparaciones controles. D-004 a 500 µg/mL inhibieron de la misma forma tanto CIK como las respuestas contráctiles-fenilefrina. Tamsulosin (0,01 mg/mL) eliminó la acción de la fenilefrina (100 % de inhibición), pero no afectó las contracciones provocadas por CIK.

Longitud del ciclo estral en ratas Sprague Dawley tratadas in útero con extracto de Roystonea regia

Gutiérrez Martínez,Ariadne; Gámez Menéndez,Rafael; Pardo Acosta,Balia; Marrero Cofiño,Gisela
Fonte: Revista Cubana de Farmacia Publicador: Revista Cubana de Farmacia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2009 ES
Relevância na Pesquisa
26.71%
El D-004 consiste en una mezcla de ácidos grasos que inhibe significativamente la hiperplasia prostática inducida por testosterona en roedores. El objetivo del presente estudio fue evidenciar los posibles efectos adversos sobre el ciclo estral de hembras F1 expuestas in útero al D-004. Se utilizaron ratas Sprague Dawley, distribuidas aleatoriamente en 4 grupos: un control y 3 tratados con D-004 a las dosis de 500, 750 y 1 000 mg/kg; las hembras recibieron la administración de la dosis por vía oral desde 15 días antes del apareo y hasta el fin de la lactancia. A una hembra por camada de la generación F1 se le estudió la citología vaginal y se calculó la longitud aproximada del ciclo, la cual no se vio afectada ya que no existieron diferencias significativas (p= 0,1537) entre los grupos tratados y el control. Estos resultados indican que el D-004 no reveló alteraciones del ciclo estral de las crías hembras expuestas in útero.

Comparación de los efectos del D-004, imipramina y sertralina en el modelo de nado forzado en ratones

Carbajal Quintana,Daisy; Molina Cuevas,Vivian; Ravelo Calzado,Yazmin; Mas Ferreiro,Rosa
Fonte: Revista Cubana de Farmacia Publicador: Revista Cubana de Farmacia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2012 ES
Relevância na Pesquisa
36.82%
Introducción: La finasterida, inhibidor de la 5 a-reductasa, se emplea en el tratamiento de la hiperplasia prostática benigna, y ocasiona entre sus efectos adversos un aumento de cuadros de depresión. El D-004 es un extracto lipídico extraído a partir del fruto de la palma real (Roystonea regia), inhibe la 5 a-reductasa y previene la hiperplasia prostática benigna, y muestra un efecto antidepresivo moderado en el ensayo de nado forzado y suspensión por la cola. Objetivo: comparar el efecto del D-004 con la imipramina y la sertralina sobre la duración de las conductas de inmovilidad, nado y escalado en el ensayo de nado forzado. Métodos: se distribuyeron los ratones en ocho grupos: control (vehículo), tres tratados con D-004 (100, 250 y 500 mg/kg), dos con sertralina y dos con imipramina (30 y 50 mg/kg) respectivamente. Estos se colocaron en un cilindro de cristal que contenía agua a una altura de 6 cm y se cuantificaron las conductas. Resultados: la administración oral de D-004 (100, 250 y 500 mg/kg) durante 14 días redujo significativamente el tiempo de inmovilidad con respecto al grupo control (17, 22 y 25 %) y aumentó significativamente la conducta de nado en 1,58, 1,68 y 1,74 veces. Este efecto resulta moderado (25 %) comparado con las reducciones alcanzadas por la sertralina y la imipramina (³ 60 %). Las dosis mayores (250 y 500 mg/kg) ocasionaron incrementos de la conducta de escalado...

Estudio del D-004 sobre la defensa antioxidante endógena en ratas con hiperplasia prostática inducida por inyección de testosterona

Pérez Guerra,Yohani; Oyarzábal Yera,Ambar; Molina Cuevas,Vivian; Jiménez Despaigne,Sonia; Curveco Sánchez,Dayisell; Mas Ferreiro,Rosa
Fonte: Revista Cubana de Farmacia Publicador: Revista Cubana de Farmacia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2010 ES
Relevância na Pesquisa
36.82%
La hiperplasia prostática benigna, enfermedad común en hombres mayores de 50 años de edad, se caracteriza por el crecimiento incontrolado de la glándula prostática y la presencia de síntomas del tracto bajo urinario. El estrés oxidativo ha sido recientemente asociado con la causa de esta enfermedad. El D-004, extracto lipídico del fruto de la Roystonea regia, ha mostrado reducir la hiperplasia prostática inducida por testosterona en roedores y producir efectos antioxidantes in vitro e in vivo, pero sus efectos sobre las enzimas del sistema antioxidante endógeno no han sido estudiados. Este trabajo investigó los efectos del tratamiento oral con D-004, durante 14 días, sobre las enzimas superóxido dismutasa y catalasa en ratas con hiperplasia prostática inducida por testosterona. Los animales se distribuyeron en 4 grupos: un control negativo y tres inyectados con testosterona: uno tratado con el vehículo (control positivo) y dos con D-004 (400 y 800 mg/kg, respectivamente). Se determinó la capacidad antioxidante total del plasma y las actividades de las enzimas superóxido dismutasa y catalasa en eritocitos lisados y plasma, respectivamente. El tratamiento oral con D-004 (400 y 800 mg/kg) previno de modo marcado y significativo el agrandamiento de la próstata inducido con testosterona en ratas...

Efecto del D-004, un extracto del fruto de Roystonea regia y omega-3 en el modelo de nado forzado en ratones

Carbajal Quintana,Daisy; Ravelo Calzado,Yazmín; Molina Cuevas,Vivian; Más Ferreiro,Rosa; Arruzazabala Valmaña,María de Lourdes
Fonte: Revista Cubana de Farmacia Publicador: Revista Cubana de Farmacia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2010 ES
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La depresión se encuentra entre las principales causas de morbilidad y mortalidad de la población adulta y su manejo farmacológico incluye, entre otras opciones alternativas, el uso de la medicina complementaria, como es el caso del aceite de pescado rico en ácidos grasos omega-3 (AGw3). El D-004, extracto lipídico de los frutos de la palma real (Roystonea regia) que consiste en una mezcla de ácidos grasos, ha mostrado poseer una moderada acción antidepresiva. El objetivo de este trabajo consistió en comparar los efectos del tratamiento oral con D-004, con los AGw3 y con su terapia combinada en el modelo del nado forzado en ratones. Para ello, ratones machos se distribuyeron en 8 grupos: uno control tratado con el vehículo, dos con D-004 (250 y 500 mg/kg ), dos con AGw3 (250 y 500 mg/kg), dos con la combinación y uno con imipramina (10 mg/kg i.p.). Todos los tratamientos disminuyeron significativamente el tiempo de inmovilidad con respecto al control, sin diferencias entre dosis similares de los respectivos tratamientos. En conclusión, el D-004, los AGw3 y su terapia combinada resultaron igualmente efectivos en reducir el tiempo de inmovilidad de los ratones, sin manifestarse efectos aditivos o sinérgicos con la administración conjunta de ambas sustancias.