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N-acetylchitooligosaccharides elicit expression of a single (1→3)-β-glucanase gene in suspension-cultured from barley (Hordeum vulgare); N-acetylchitooligosaccharides elicit expression of a single (1->3)-beta-glucanase gene in suspension-cultured from barley (Hordeum vulgare)

Kaku, Hanae; Shibuya, Naoto; Xu, P.; Aryan, Arun Prakash; Fincher, Geoffrey B.
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Artigo de Revista Científica
Publicado em //1997 EN
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N-acetylchitooligosaccharides of degree of polymerization 6 to 8 elicit the synthesis of (1→3)-β-glucan endohydrolase (EC 3.2.1.39) activity in suspension-cultured cells derived from immature barley (Hordeum vulgare) embryos. Corresponding de-acety-lated chitooligosaccharides have no effect. Concentrations of N-acetylchitoheptaose in the 200 nM range are sufficient to elicit the response. A 2- to 3-fold increase in (1→3)-β-glucanase activity is detected 24 h after the addition of the oligosaccharide. Non-denaturing gel electrophoresis, coupled with the in situ detection of (1→3)-β-glucanase activity in the gels, has enabled the separation of specific isoforms of the enzyme. The increase in (1→3)-β-glucanase activity following N-acetylchitoheptaose induction is attributable predominantly to enhanced levels of isoenzyme GII, although the barley (1→3)-β-glucanase gene family encodes at least seven different isoforms of the enzyme. Northern hybridization analyses with gene-specific probes confirmed the presence of mRNA encoding isoenzyme GII as the major mRNA in cells treated with the oligosaccharide elicitor. The results therefore demonstrate a specific induction of the (1→3)-β-glucanase isoenzyme GII gene following stimulation of barley cells with oligosaccharides of fungal cell wall origin...

Radiolabeling of lipo-chitooligosaccharides using the NodH sulfotransferase: a two-step enzymatic procedure

Gressent, Frédéric; Cullimore, Julie V; Ranjeva, Raoul; Bono, Jean-Jacques
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 13/04/2004 EN
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Changes in Soybean Global Gene Expression after Application of Lipo-Chitooligosaccharide from Bradyrhizobium japonicum under Sub-Optimal Temperature

Wang, Nan; Khan, Wajahatullah; Smith, Donald L.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 13/02/2012 EN
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Lipo-chitooligosaccharides (LCOs), signal compounds produced by N2-fixing rhizobacteria after isoflavone induction, initiate nodule formation in host legumes. Given LCOs' structural similarity to pathogen-response-eliciting chitin oligomers, foliar application of LCOs was tested for ability to induce stress-related genes under optimal growth conditions. In order to study the effects of LCO foliar spray under stressed conditions, soybean (Glycine max) seedlings grown at optimal temperature were transferred to sub-optimal temperature. After a 5-day acclimation period, the first trifoliate leaves were sprayed with 10−7 M LCO (NodBj-V (C18∶1, MeFuc)) purified from genistein-induced Bradyrhizobium japonicum culture, and harvested at 0 and 48 h following treatment. Microarray analysis was performed using Affymetrix GeneChip® Soybean Genome Arrays. Compared to the control at 48 h after LCO treatment, a total of 147 genes were differentially expressed as a result of LCO treatment, including a number of stress-related genes and transcription factors. In addition, during the 48 h time period following foliar spray application, over a thousand genes exhibited differential expression, including hundreds of those specific to the LCO-treated plants. Our results indicated that the dynamic soybean foliar transcriptome was highly responsive to LCO treatment. Quantitative real-time PCR (qPCR) validated the microarray data.

Chitooligosaccharides modified by maillard reaction with glucose inhibit adhesion of enteropathogenic bacteria to mucin

Montenegro, M. I.; Cardelle-Cobas, A.; Gullón, P.; Tavaria, Freni; Pintado, M. E.; Gullón, B.
Fonte: Universidade Católica Portuguesa Publicador: Universidade Católica Portuguesa
Tipo: Artigo de Revista Científica
Publicado em //2013 ENG
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