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O impacto macroeconómico das contribuições para a Segurança Social

Lopes, Suéli Domingues
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Dissertação de Mestrado
Publicado em //2013 POR
Relevância na Pesquisa
27.24%
Dissertação de mestrado em Economia Monetária, Bancária e Financeira; Neste trabalho, pretende-se estudar o efeito das contribuições para a Segurança Social sobre o crescimento económico e o seu papel em termos de estabilização económica. Adicionalmente, procura-se analisar o impacto das contribuições para a Segurança Social para diferentes horizontes temporais. Finalmente, avalia-se a existência de efeitos crowding-in e/ou de efeitos crowding-out das contribuições para a Segurança Social sobre a despesa do sector privado. Para a elaboração deste trabalho, utilizou-se uma amostra de 32 países com dados relativos ao período de 1960 a 2007. Os resultados sugerem que as contribuições para a Segurança Social desempenham um papel importante enquanto estabilizadores automáticos nos países Europeus, nos países da OCDE e nos países de rendimento elevado. A evidência empírica mostra ainda que, embora um aumento da componente discricionária das contribuições para a Segurança Social não afecte significativamente o crescimento do produto, o impacto sobre a composição deste é relevante, em particular, dado o efeito positivo sobre o crescimento do investimento privado no longo-prazo.; This paper aims at studying the effect of Social Security contributions on economic growth and their role in terms of economic stabilization. Additionally...

Crowding is directed to the fovea and preserves only feature contrast

Petrov, Yury; Popple, Ariella V.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 06/03/2007 EN
Relevância na Pesquisa
27.24%
The abundant literature on crowding offers fairly simple explanations for the phenomenon, such as position uncertainty or feature pooling, but convincing evidence to support these explanations is lacking. In part, this is because the stimuli used for crowding studies are usually letters or other complex shapes, which makes it hard to determine exactly what kind of information is lost. In our experiment, we asked observers to identify simultaneously the slants (left or right) of three horizontally aligned Gabor targets. The targets were presented at 6° in the periphery, and their size and separation were chosen to incur strong crowding. The loss of information about the position or orientation of individual members of the Gabor triads does not explain our results. Instead, crowding appears to be a particular form of collective information loss. Firstly, the outmost target was crowded much less than the other targets, which rules out explanations based on simple pooling and shows that crowding has a pronounced foveal directionality. Secondly, the specific pattern of confusion shown by all the observers indicates that the only reliable information available to them was orientation contrast, that is, the number (and, to a lesser degree...

Influence of Macromolecular Crowding on Protein-Protein Association Rates—a Brownian Dynamics Study

Wieczorek, Grzegorz; Zielenkiewicz, Piotr
Fonte: The Biophysical Society Publicador: The Biophysical Society
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.24%
The high total concentration of macromolecules, often referred to as macromolecular crowding, is one of the characteristic features of living cells. Macromolecular crowding influences interactions between many types of macromolecules, with consequent effects on, among others, the rates of reactions occurring in the cell. Simulations to study the influence of crowding on macromolecular association rate were performed using a modified Brownian dynamics protocol. The calculated values of the time-dependent self-diffusion coefficients in different crowding conditions are in a very good agreement with those obtained by other authors. Simulations of the complex formation between the monoclonal antibody HyHEL-5 and its antigen hen egg lysozyme, both represented at atomic level detail, show that the calculated association rates strongly depend on the volume excluded by crowding. The rate obtained for the highest concentration of crowding particles is greater than twofold higher than the rate for proteins without crowding.

Effect of Macromolecular Crowding on Protein Folding Dynamics at the Secondary Structure Level

Mukherjee, Smita; Waegele, Matthias M.; Chowdhury, Pramit; Guo, Lin; Gai, Feng
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
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Macromolecular crowding is one of the key characteristics of the cellular environment and therefore, is intimately coupled to the process of protein folding in vivo. While previous studies have provided invaluable insight into the effect of crowding on the stability and folding rate of protein tertiary structures, very little is known about how crowding affects protein folding dynamics at the secondary structure level. Herein, we examine the thermal stability and folding-unfolding kinetics of three small folding motifs, i.e., a 34-residue α-helix, a 34-residue cross-linked helix-turn-helix, and a 16-residue β hairpin, in the presence of two commonly used crowding agents, Dextran 70 (200 g/L) and Ficoll 70 (200 g/L). We find that these polymers do not induce any appreciable changes in the folding kinetics of the two helical peptides, which is somewhat surprising as the helix-coil transition kinetics have been shown to depend on viscosity. Also to our surprise and in contrast to what has been observed for larger proteins, we find that crowding leads to an appreciable decrease in the folding rate of the shortest β-hairpin peptide, indicating that besides the excluded volume effect, other factors also need to be considered when evaluating the net effect of crowding on protein folding kinetics. A model considering both the static and dynamic effects arising from the presence of the crowding agent is proposed to rationalize these results.

Dissociable effects of attention and crowding on orientation averaging

Dakin, Steven C.; Bex, Peter J.; Cass, John R.; Watt, Roger J.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 29/10/2009 EN
Relevância na Pesquisa
27.24%
It has been proposed that visual crowding—the breakdown in recognition that occurs when objects are presented in cluttered scenes—reflects a limit imposed by visual attention. We examined this idea in the context of an orientation averaging task, having subjects judge the mean orientation of a set of oriented signal elements either in isolation, or “crowded” by nearby randomly oriented elements. In some conditions, subjects also had to perform an attentionally demanding secondary task. By measuring performance at different levels of signal orientation variability, we show that crowding increases subjects’ local uncertainty (about the orientation of individual elements) but that diverting attention reduces their global efficiency (the effective number of elements they can average over). Furthermore, performance with the same stimulus-sequence, presented multiple times, reveals that crowding does not induce more stimulus-independent variability (as would be predicted by some accounts based on attention). We conclude that crowding and attentional load have dissociable perceptual consequences for orientation averaging, suggesting distinct neural mechanisms for both. For the task we examined, attention can modulate the effects of crowding by changing the efficiency with which information is analyzed by the visual system but since crowding changes local uncertainty...

Visual Crowding: a fundamental limit on conscious perception and object recognition

Whitney, David; Levi, Dennis M.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.24%
Crowding, the inability to recognize objects in clutter, sets a fundamental limit on conscious visual perception and object recognition throughout most of the visual field. Despite how widespread and essential it is to object recognition, reading, and visually guided action, a solid operational definition of what crowding is has only recently become clear. The goal of this review is to provide a broad-based synthesis of the most recent findings in this area, to define what crowding is and is not, and to set the stage for future work that will extend crowding well beyond low-level vision. Here we define five diagnostic criteria for what counts as crowding, and further describe factors that both escape and break crowding. All of these lead to the conclusion that crowding occurs at multiple stages in the visual hierarchy.

Reexamining the possible benefits of visual crowding: dissociating crowding from ensemble percepts

Bulakowski, Paul F.; Post, Robert B.; Whitney, David
Fonte: Springer-Verlag Publicador: Springer-Verlag
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.24%
Peripheral objects and their features become indistinct when closely surrounding but nonoverlapping objects are present. Most models suggest that this phenomenon, called crowding, reflects limitations of visual processing, but an intriguing idea is that it may be, in part, adaptive. Specifically, the mechanism generating crowding may simultaneously facilitate ensemble representations of features, leaving meaningful information about clusters of objects. In two experiments, we tested whether visual crowding and the perception of ensemble features share a common mechanism. Observers judged the orientation of a crowded bar, or the ensemble orientation of all bars in the upper and lower visual fields. While crowding was predictably stronger in the upper relative to the lower visual field, the ensemble percept did not vary between the visual fields. Featural averaging within the crowded region does not always scale with the resolution limit defined by crowding, suggesting that dissociable processes contribute to visual crowding and ensemble percepts.

Object crowding

Wallace, Julian M.; Tjan, Bosco S.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.24%
Crowding occurs when stimuli in the peripheral fields become harder to identify when flanked by other items. This phenomenon has been demonstrated extensively with simple patterns (e.g., Gabors and letters). Here, we characterize crowding for everyday objects. We presented three-item arrays of objects and letters, arranged radially and tangentially in the lower visual field. Observers identified the central target, and we measured contrast energy thresholds as a function of target-to-flanker spacing. Object crowding was similar to letter crowding in spatial extent but was much weaker. The average elevation in threshold contrast energy was in the order of 1 log unit for objects as compared to 2 log units for letters and silhouette objects. Furthermore, we examined whether the exterior and interior features of an object are differentially affected by crowding. We used a circular aperture to present or exclude the object interior. Critical spacings for these aperture and “donut” objects were similar to those of intact objects. Taken together, these findings suggest that crowding between letters and objects are essentially due to the same mechanism, which affects equally the interior and exterior features of an object. However, for objects defined with varying shades of gray...

Perceptual Learning Reduces Crowding in Amblyopia and in the Normal Periphery

Hussain, Zahra; Webb, Ben S.; Astle, Andrew T.; McGraw, Paul V.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 11/01/2012 EN
Relevância na Pesquisa
27.27%
Amblyopia is a developmental visual disorder of cortical origin, characterized by crowding and poor acuity in central vision of the affected eye. Crowding refers to the adverse effects of surrounding items on object identification, common only in normal peripheral but not central vision. We trained a group of adult human amblyopes on a crowded letter identification task to assess whether the crowding problem can be ameliorated. Letter size was fixed well above the acuity limit, and letter spacing was varied to obtain spacing thresholds for central target identification. Normally sighted observers practiced the same task in their lower peripheral visual field. Independent measures of acuity were taken in flanked and unflanked conditions before and after training to measure crowding ratios at three fixed letter separations. Practice improved the letter spacing thresholds of both groups on the training task, and crowding ratios were reduced after posttest. The reductions in crowding in amblyopes were associated with improvements in standard measures of visual acuity. Thus, perceptual learning reduced the deleterious effects of crowding in amblyopia and in the normal periphery. The results support the effectiveness of plasticity-based approaches for improving vision in adult amblyopes and suggest experience-dependent effects on the cortical substrates of crowding.

Effect of crowding by Ficolls on OmpA and OmpT refolding and membrane insertion

Ye, Cui; Chai, Qian; Zhong, Meng; Wei, Yinan
Fonte: Wiley Subscription Services, Inc., A Wiley Company Publicador: Wiley Subscription Services, Inc., A Wiley Company
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.24%
Folding of outer membrane proteins (OMPs) has been studied extensively in vitro. However, most of these studies have been conducted in dilute buffer solution, which is different from the crowded environment in the cell periplasm, where the folding and membrane insertion of OMPs actually occur. Using OmpA and OmpT as model proteins and Ficoll 70 as the crowding agent, here we investigated the effect of the macromolecular crowding condition on OMP membrane insertion. We found that the presence of Ficoll 70 significantly slowed down the rate of membrane insertion of OmpA while had little effect on those of OmpT. To investigate if the soluble domain of OmpA slowed down membrane insertion in the presence of the crowding agent, we created a truncated OmpA construct that contains only the transmembrane domain (OmpA171). In the absence of crowding agent, OmpA171 refolded at a similar rate as OmpA, although with decreased efficiency. However, under the crowding condition, OmpA171 refolded significantly faster than OmpA. Our results suggest that the periplasmic domain slows down the rate, while improves the efficiency, of OmpA folding and membrane insertion under the crowding condition. Such an effect was not obvious when refolding was studied in buffer solution in the absence of crowding.

The same binding in contour integration and crowding

Chakravarthi, Ramakrishna; Pelli, Denis G.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 14/07/2011 EN
Relevância na Pesquisa
27.24%
Binding of features helps object recognition in contour integration, but hinders it in crowding. In contour integration, aligned adjacent objects group together to form a path. In crowding, flanking objects make the target unidentifiable. But, to date, the two tasks have only been studied separately. May and Hess (2007) suggested that the same binding mediates both tasks. To test this idea, we ask observers to perform two different tasks with the same stimulus. We present oriented grating patches that form a “snake letter” in the periphery. Observers report either the identity of the whole letter (contour integration task) or the phase of one of the grating patches (crowding task). We manipulate the strength of binding between gratings by varying the alignment between them, i.e. the Gestalt goodness of continuation, measured as “wiggle”. We find that better alignment strengthens binding, which improves contour integration and worsens crowding. Observers show equal sensitivity to alignment in these two very different tasks, suggesting that the same binding mechanism underlies both phenomena. It has been claimed that grouping among flankers reduces their crowding of the target. Instead, we find that these published cases of weak crowding are due to weak binding resulting from target-flanker misalignment. We conclude that crowding is mediated solely by the grouping of flankers with the target and is independent of grouping among flankers.

Holistic crowding: Selective interference between configural representations of faces in crowded scenes

Louie, Elizabeth G.; Bressler, David W.; Whitney, David
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 26/11/2007 EN
Relevância na Pesquisa
27.27%
It is difficult to recognize an object that falls in the peripheral visual field; it is even more difficult when there are other objects surrounding it. This effect, known as crowding, could be due to interactions between the low-level parts or features of the surrounding objects. Here, we investigated whether crowding can also occur selectively between higher level object representations. Many studies have demonstrated that upright faces, unlike most other objects, are coded holistically. Therefore, in addition to featural crowding within a face (M. Martelli, N. J. Majaj, & D. G. Pelli, 2005), we might expect an additional crowding effect between upright faces due to interference between the higher level holistic representations of these faces. In a series of experiments, we tested this by presenting an upright target face in a crowd of additional upright or inverted faces. We found that recognition was more strongly impaired when the target face was surrounded by upright compared to inverted flanker (distractor) faces; this pattern of results was absent when inverted faces and non-face objects were used as targets. This selective crowding of upright faces by other upright faces only occurred when the target–flanker separation was less than half the eccentricity of the target face...

Applying Molecular Crowding Models to Simulations of Virus Capsid Assembly In Vitro

Smith, Gregory R.; Xie, Lu; Lee, Byoungkoo; Schwartz, Russell
Fonte: The Biophysical Society Publicador: The Biophysical Society
Tipo: Artigo de Revista Científica
Publicado em 07/01/2014 EN
Relevância na Pesquisa
27.24%
Virus capsid assembly has been widely studied as a biophysical system, both for its biological and medical significance and as an important model for complex self-assembly processes. No current technology can monitor assembly in detail and what information we have on assembly kinetics comes exclusively from in vitro studies. There are many differences between the intracellular environment and that of an in vitro assembly assay, however, that might be expected to alter assembly pathways. Here, we explore one specific feature characteristic of the intracellular environment and known to have large effects on macromolecular assembly processes: molecular crowding. We combine prior particle simulation methods for estimating crowding effects with coarse-grained stochastic models of capsid assembly, using the crowding models to adjust kinetics of capsid simulations to examine possible effects of crowding on assembly pathways. Simulations suggest a striking difference depending on whether or not a system uses nucleation-limited assembly, with crowding tending to promote off-pathway growth in a nonnucleation-limited model but often enhancing assembly efficiency at high crowding levels even while impeding it at lower crowding levels in a nucleation-limited model. These models may help us understand how complicated assembly systems may have evolved to function with high efficiency and fidelity in the densely crowded environment of the cell.

Macromolecular Crowding as a Regulator of Gene Transcription

Matsuda, Hiroaki; Putzel, Gregory Garbès; Backman, Vadim; Szleifer, Igal
Fonte: The Biophysical Society Publicador: The Biophysical Society
Tipo: Artigo de Revista Científica
Publicado em 15/04/2014 EN
Relevância na Pesquisa
27.24%
Studies of macromolecular crowding have shown its important effects on molecular transport and interactions in living cells. Less clear is the effect of crowding when its influence is incorporated into a complex network of interactions. Here, we explore the effects of crowding in the cell nucleus on a model of gene transcription as a network of reactions involving transcription factors, RNA polymerases, and DNA binding sites for these proteins. The novelty of our approach is that we determine the effects of crowding on the rates of these reactions using Brownian dynamics and Monte Carlo simulations, allowing us to integrate molecular-scale information, such as the shapes and sizes of each molecular species, into the rate equations of the model. The steady-state cytoplasmic mRNA concentration shows several regimes with qualitatively different dependences on the volume fraction, ϕ, of crowding agents in the nucleus, including a broad range of parameter values where it depends nonmonotonically on ϕ, with maximum mRNA production occurring at a physiologically relevant value. The extent of this crowding dependence can be modulated by a variety of means, suggesting that the transcriptional output of a gene can be regulated jointly by the local level of macromolecular crowding in the nucleus...

CaMKII Binding to GluN2B Is Differentially Affected by Macromolecular Crowding Reagents

Goodell, Dayton J.; Eliseeva, Tatiana A.; Coultrap, Steven J.; Bayer, K. Ulrich
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 05/05/2014 EN
Relevância na Pesquisa
27.27%
Binding of the Ca2+/calmodulin(CaM)-dependent protein kinase II (CaMKII) to the NMDA-type glutamate receptor (NMDAR) subunit GluN2B controls long-term potentiation (LTP), a form of synaptic plasticity thought to underlie learning and memory. Regulation of this interaction is well-studied biochemically, but not under conditions that mimic the macromolecular crowding found within cells. Notably, previous molecular crowding experiments with lysozyme indicated an effect on the CaMKII holoenzyme conformation. Here, we found that the effect of molecular crowding on Ca2+/CaM-induced CaMKII binding to immobilized GluN2B in vitro depended on the specific crowding reagent. While binding was reduced by lysozyme, it was enhanced by BSA. The ATP content in the BSA preparation caused CaMKII autophosphorylation at T286 during the binding reaction; however, enhanced binding was also observed when autophosphorylation was blocked. Importantly, the positive regulation by nucleotide and BSA (as well as other macromolecular crowding reagents) did not alleviate the requirement for CaMKII stimulation to induce GluN2B binding. The differential effect of lysozyme (14 kDa) and BSA (66 kDa) was not due to size difference, as both dextran-10 and dextran-70 enhanced binding. By contrast...

Macaque monkeys experience visual crowding

Crowder, Erin A.; Olson, Carl R.
Fonte: The Association for Research in Vision and Ophthalmology Publicador: The Association for Research in Vision and Ophthalmology
Tipo: Artigo de Revista Científica
Publicado em 24/04/2015 EN
Relevância na Pesquisa
27.27%
In peripheral vision, objects that are easily discriminated on their own become less discriminable in the presence of surrounding clutter. This phenomenon is known as crowding.The neural mechanisms underlying crowding are not well understood. Better insight might come from single-neuron recording in nonhuman primates, provided they exhibit crowding; however, previous demonstrations of crowding have been confined to humans. In the present study, we set out to determine whether crowding occurs in rhesus macaque monkeys. We found that animals trained to identify a target letter among flankers displayed three hallmarks of crowding as established in humans. First, at a given eccentricity, increasing the spacing between the target and the flankers improved recognition accuracy. Second, the critical spacing, defined as the minimal spacing at which target discrimination was reliable, was proportional to eccentricity. Third, the critical spacing was largely unaffected by object size. We conclude that monkeys, like humans, experience crowding. These findings open the door to studies of crowding at the neuronal level in the monkey visual system.

Image correlates of crowding in natural scenes

Wallis, Thomas S. A.; Bex, Peter J.
Fonte: The Association for Research in Vision and Ophthalmology Publicador: The Association for Research in Vision and Ophthalmology
Tipo: Artigo de Revista Científica
Publicado em 13/07/2012 EN
Relevância na Pesquisa
27.27%
Visual crowding is the inability to identify visible features when they are surrounded by other structure in the peripheral field. Since natural environments are replete with structure and most of our visual field is peripheral, crowding represents the primary limit on vision in the real world. However, little is known about the characteristics of crowding under natural conditions. Here we examine where crowding occurs in natural images. Observers were required to identify which of four locations contained a patch of “dead leaves' (synthetic, naturalistic contour structure) embedded into natural images. Threshold size for the dead leaves patch scaled with eccentricity in a manner consistent with crowding. Reverse correlation at multiple scales was used to determine local image statistics that correlated with task performance. Stepwise model selection revealed that local RMS contrast and edge density at the site of the dead leaves patch were of primary importance in predicting the occurrence of crowding once patch size and eccentricity had been considered. The absolute magnitudes of the regression weights for RMS contrast at different spatial scales varied in a manner consistent with receptive field sizes measured in striate cortex of primate brains. Our results are consistent with crowding models that are based on spatial averaging of features in the early stages of the visual system...

Le rôle des fréquences spatiales dans l’effet d’encombrement en identification de lettres

Zahabi, Sacha
Fonte: Université de Montréal Publicador: Université de Montréal
Tipo: Thèse ou Mémoire numérique / Electronic Thesis or Dissertation
FR
Relevância na Pesquisa
27.24%
L’effet d’encombrement, qui nous empêche d’identifier correctement un stimulus visuel lorsqu’il est entouré de flanqueurs, est omniprésent à travers une grande variété de classes de stimuli. L’excentricité du stimulus cible ainsi que la distance cible-flanqueur constituent des facteurs fondamentaux qui modulent l’effet d’encombrement. La similarité cible-flanqueur semble également contribuer à l’ampleur de l’effet d’encombrement, selon des données obtenues avec des stimuli non-linguistiques. La présente étude a examiné ces trois facteurs en conjonction avec le contenu en fréquences spatiales des stimuli, dans une tâche d’identification de lettres. Nous avons présenté des images filtrées de lettres à des sujets non-dyslexiques exempts de troubles neurologiques, tout en manipulant l’excentricité de la cible ainsi que la similarité cible-flanqueurs (selon des matrices de confusion pré-établies). Quatre types de filtrage de fréquences spatiales ont été utilisés : passe-bas, passe-haut, à large bande et mixte (i.e. élimination des fréquences moyennes, connues comme étant optimales pour l’identification de lettres). Ces conditions étaient appariées en termes d’énergie de contraste. Les sujets devaient identifier la lettre cible le plus rapidement possible en évitant de commettre une erreur. Les résultats démontrent que la similarité cible-flanqueur amplifie l’effet d’encombrement...

FEELING OF CROWDING AND PSYCHIATRIC DISORDERS

Kamal, Preet; Gupta, I.D.
Fonte: Medknow Publications Publicador: Medknow Publications
Tipo: Artigo de Revista Científica
Publicado em //1988 EN
Relevância na Pesquisa
27.24%
Relationship of high population density to mental illness has been well known. The feeling of crowding has also been found to have a causal influence on individual's mental health. Recently, it has been reported that even in high population density, the individuals w ho have a high feeling of crowding are more prone to mental illness. However, no attempt has been made by Indian researchers to study whether the amount of feeling of crowding which is supposed to be a cause of mental illness in high dense areas differs in various, psychiatric disorders. Thus, a prospective study which included 150 psychiatric patients suffering from schizophrenia, affective disorders and neuroses and 50 normal subjects, selected from high dense area of Bikaner city was taken up to find out (1) whether the amount of feeling of crowding differs in schizophrenia, affective disorders, neuroses and normal, and (2) whether the severity of illness in these subjects has any relationship of the feeling of crowding experienced by them. Results revealed that feeling of crowding differs in schizophrenics, patients with affective disorders, neurotics and normal subjects. The scores of feeling of crowding and severity of illness of all these subjects had a positive relationship. Results and their implications are discussed.

Entropic stabilization of the folded states of RNA due to macromolecular crowding

Denesyuk, Natalia A.; Thirumalai, D.
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 15/04/2013
Relevância na Pesquisa
27.27%
We review the effects of macromolecular crowding on the folding of RNA by considering the simplest scenario when excluded volume interactions between crowding particles and RNA dominate. Using human telomerase enzyme as an example, we discuss how crowding can alter the equilibrium between pseudoknot and hairpin states of the same RNA molecule - a key aspect of crowder-RNA interactions. We summarize data showing that the crowding effect is significant only if the size of the spherical crowding particle is smaller than the radius of gyration of the RNA in the absence of crowding particles. The implication for function of the wild type and mutants of human telomerase is outlined by using a relationship between enzyme activity and its conformational equilibrium. In addition, we discuss the interplay between macromolecular crowding and ionic strength of the RNA buffer. Finally, we briefly review recent experiments which illustrate the connection between excluded volume due to macromolecular crowding and the thermodynamics of RNA folding.