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Efici??ncia reprodutiva com insemina????o artificial intra-cervical e p??s-cervical, em f??meas su??nas, associada ?? concentra????o esperm??tica e perfil estral; Reproductive efficiency with intra-cervical and post-cervical artificial insemination in female swine associated with spermatozoa concentration and estrus profile

SERRET, Carolina Gon??alves
Fonte: Universidade Federal de Pelotas; Biotecnologia; Programa de P??s-Gradua????o em Biotecnologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Biotecnologia; Programa de P??s-Gradua????o em Biotecnologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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Artificial insemination (AI) is a technique intensively used in the swine farms nowadays, due to its benefits related to genetic improvement and health status, allowing the industry to market high-quality healthy products. The post-cervical AI (PCAI) allows the deposition of semen in the uterine body, using 1 x 10 9 spermatozoa dose. Thus, with PCAI the fertility of one particular boar can influence the reproductive performance of a much higher number of females than in ICAI-based systems. The objective of this study was to compare the subsequent reproductive performance of swine breeding females submitted to either intra-cervical artificial insemination (ICAI) with sperm concentrations of 3.5 x 10 9 spermatozoa per dose or post-cervical artificial insemination (PCAI) with 2, 1, or 0.5 x 10 9 spermatozoa per dose. The study was conducted in a commercial farm including 338 F1 females. Among them, 232 were first inseminated 12 h after estrus detection, receiving the subsequent doses after 12 h intervals, whereas 106 were inseminated right after ovulation diagnosis through real-time ultrasound. Conception and farrowing (FR) rates for ICAI (98.9% e 97.9%, respectively) did not differ (P < 0,001) from those observed for PCAI (87.1% e 84.3%...

Efeito do jejum e da insulina ex??gena sobre par??metros metab??licos e express??o g??nica do receptor do horm??nio do crescimento (GH) e fator de crescimento semelhante ?? insulina tipo I (IGF-I), no fol??culo pr??ovulat??rio de ovelhas; Effects of fasting and exogenous insulin on metabolic parameters and gene expression of growth hormone receptor (GHR) and insulin like growth factor I (IGF-I), in the pre-ovulatory follicles of ewes

SCHNEIDER, Augusto
Fonte: Universidade Federal de Pelotas; Biotecnologia; Programa de P??s-Gradua????o em Biotecnologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Biotecnologia; Programa de P??s-Gradua????o em Biotecnologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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In non-ovarian tissues GHR expression is regulated by insulin concentrations and is correlated to IGF-I expression. In the ovary IGF-I expression is also related to insulin levels, however no correlation with GHR was demonstrated until now. The aim of this study was to investigate the effect of fasting or insulin administration on blood concentrations of glucose, nonsterified fatty acids, insulin, insulin like growth factor I (IGF-I) and estradiol and on follicular expression of growth hormone receptor (GHR) and IGF-I mRNA in ewes. Fifteen ewes received an intravaginal progesterone releasing device that was removed 6 days later (Day 0). In Day -2 the ewes were divided in: control group, which received maintenance diet, insulin group, which received insulin injections every 12 hours from Day -2 to 2 and fasting group, which was submitted to fasting from Day -2 to 2. The results of the current study revealed that insulin administration or fasting during the development of a follicular wave did not affect (P=0.22) follicular diameter, although insulin injection increased (P=0.02) estradiol production. There was no difference among groups for GHR or IGF-I mRNA expression in granulosa (P=0.62, 0.43) or theca cells (P=0.92, 0.43). For fasting group there was a positive correlation between glucose and estradiol (r=0.97...

Variabilidade gen??tica e a efici??ncia de sele????o no car??ter dorm??ncia de sementes em aveia-preta(Avena strigosa Schreb.); Genetic variability and breeding efficiency for seed dormancy in blac oat (Avena strigosa Schreb.)

MOLITERNO, Enrique Alfredo Parachu
Fonte: Universidade Federal de Pelotas; Agronomia; Programa de P??s-gradua????o em Ci??ncia e Tecnologia de Sementes; UFPel; BR Publicador: Universidade Federal de Pelotas; Agronomia; Programa de P??s-gradua????o em Ci??ncia e Tecnologia de Sementes; UFPel; BR
Tipo: Tese de Doutorado Formato: application/pdf
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Seed dormancy is a trait shown by a large variety of weedy plants, which helps the purpose of perpetuating the species through space and time by delaying germination until specific environmental cues happen. Black oat, a temperate forage grass, is widely used for pasture and as a cover crop in minimum tillage systems in Southern Brazil. However, the largest portion of the seed sown belongs to an old variety, which has no genetic identity, contributing to the appearance of undesirable agronomic traits in a crop species such as seed dormancy. This trait is hold responsible for turning black oat into a potential weedy species in areas sown to other cool season cereals, such as wheat and barley. Three methods were used to screen and select for black oat genotypes expressing low seed dormancy, i.e. screening of lines collected throughout different agricultural regions of the state of Rio Grande do Sul; subjecting a specific line of the species to the effects of two chemical and one physical mutagens and crossbreeding between selected lines and commercial cultivars of the species. All three methods were undertaken under a glasshouse environment (without temperature control), and since there are no known vegetative morphological traits associated to seed dormancy the procedure consisted on selecting seedlings from non dormant seeds. These were grown in the glasshouse environment and their progeny seeds tested for germinability...

Jovens e a produ????o de subjetividades: vidas na festa, vidas na escola; Jovens e a produ????o de subjetividades: vidas na festa, vidas na escola

Balinhas, Vera L??cia Gainssa
Fonte: Universidade Federal de Pelotas; Educa????o; Programa de P??s-Gradua????o em Educa????o; UFPel; BR Publicador: Universidade Federal de Pelotas; Educa????o; Programa de P??s-Gradua????o em Educa????o; UFPel; BR
Tipo: Tese de Doutorado Formato: application/pdf
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This thesis focuses on the relationship between parties and school in the constitution of subjectivities of the youth. This is an ethnographic research conducted with students at the final levels of the basic education in a public school, in Cassino Beach, southern Brazil. The study is inspired primarily by the readings of Foucault and several contemporary authors dealing with the youth theme. The students pointed out to dichotomous perceptions between the school and the party: the party as a place of pleasure, fun, freedom and experimentation, while the school meant continuity, routine, obligation to do something, a demand coming from the family, a place where there is more standardization of conduct than acceptance of difference. The learning and school knowledge proved related with a promising future, engaged with speeches of sacrifices and rewards, with the postponement of satisfaction of desires and needs, and rarely with the will and pleasure for knowledge. The satisfaction in space and time appeared more linked to experiences and knowledge, which referred to the elements, contexts and festive situations. By unravelling the boundaries of the parties and the school it was possible to think and realize that, at the school, the promises that are made to motivate the pace...

A Escola de Belas Artes de Pelotas: da funda????o ?? federaliza????o (1949-1972) uma contribui????o para a hist??ria da educa????o em Pelotas; The present study is a historic research about an institution of university level: the Escola de Belas Artes de Pelotas (EBA), nowadays Instituto de Artes e Design of the Universidade Federal de Pelotas (IAD UFPEL). The investigation focus is the origin and the first 23 years of the institution, that is, from 1949, the year of its foundation, as a private course, until 1972, when it became a federal public educational institution. The work is carried out within the scope of the Cultural History theory proposition. The method adopted is that of documental research, mainly of the institution archives, of a private archive and through personal interviews conducted by the researcher. The findings of the research are treated according to the theoretic references adopted. The work investigates and analyses the conditions that leaded to the creation of this institution and also its trajectory until it became a federal institution (thus, in a parallel way, the study reveals elements that constructed the identity of the institution). To attempt to understand such a process, the work makes a recollection of the history of Pelotas, of the history of art teaching institutions, and investigates the participation of personalities that probably had decisive influence over the implementation of the studied art course. With the information obtained through this research it has been possible to infer that the advent of the school in 1949 occurred as a result of a combination of factors, such as the context ( the city of Pelotas ) and the action of individuals, as Dona Marina de Moraes Pires - whose efforts to offer to the city an art course of university level made the difference - and the Italian painter Aldo Locatelli the first painting teacher of the art course.

MAGALH??ES, Clarice Rego
Fonte: Universidade Federal de Pelotas; Educa????o; Programa de P??s-Gradua????o em Educa????o; UFPel; BR Publicador: Universidade Federal de Pelotas; Educa????o; Programa de P??s-Gradua????o em Educa????o; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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O presente estudo constitui-se em pesquisa hist??rica a respeito de uma institui????o de ensino superior: A Escola de Belas Artes de Pelotas (EBA), atual Instituto de Artes e Design da Universidade Federal de Pelotas (IAD-UFPEL). O foco da investiga????o ?? o surgimento e os primeiros 23 anos da institui????o, ou seja, do ano de 1949, data da funda????o, como curso particular, at?? a federaliza????o, em 1972, quando passa a ser uma institui????o p??blica de ensino. O trabalho ?? realizado dentro da proposta te??rica da Hist??ria Cultural. O m??todo empregado ?? a pesquisa documental, e s??o utilizados principalmente os arquivos da institui????o, um arquivo particular e entrevistas realizadas pela pesquisadora. Estes achados da pesquisa s??o abordados de acordo com o referencial te??rico adotado. O trabalho investiga e analisa as condi????es que proporcionaram a cria????o desta institui????o e tamb??m a sua trajet??ria at?? a federaliza????o (assim, paralelamente, o estudo traz elementos construtores de identidade da institui????o). Para tentar entender este processo, faz um apanhado da hist??ria da cidade de Pelotas, da hist??ria das institui????es de ensino de arte e investiga a participa????o de personalidades que teriam influ??do decisivamente na implementa????o do curso em estudo. Com as informa????es trazidas por esta pesquisa...

No meio do caminho: entre o discurso adolescente e a norma; In the middle of the way: between the adolescent speech and the norm

TEIXEIRA, Fabiane Lopes
Fonte: Universidade Federal de Pelotas; Educa????o; Programa de P??s-Gradua????o em Educa????o; UFPel; BR Publicador: Universidade Federal de Pelotas; Educa????o; Programa de P??s-Gradua????o em Educa????o; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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Esta disserta????o teve como objetivo investigar como alguns/algumas adolescentes v??m construindo suas diversas possibilidades identit??rias e dentre elas as de g??nero a partir do dispositivo da sexualidade. Para tanto, analisei narrativas de alguns/algumas adolescentes, que eram meus alunos e minhas alunas das oitavas s??ries, no ano de 2007, numa Escola Municipal de Ensino Fundamental da cidade do Rio Grande/RS. Essas narrativas foram produzidas a partir de estrat??gias de fazer falar , por mim adotadas, que consistiram de atividades desenvolvidas com os/as alunos/as a respeito da tem??tica da Sexualidade. No estudo, estabeleci conex??es com os Estudos Culturais, nas suas vertentes p??s-estruturalistas, dialogando com autores como Bauman, Fischer, Hall, Louro, Silva, Veiga-Neto, entre outros; al??m de tomar empr??stimo de algumas preposi????es de Foucault. Entendo que as diversas institui????es tais como a fam??lia e a escola, al??m dos artefatos culturais que funcionam como uma pedagogia cultural direcionados aos/??s adolescentes acabam produzindo esses corpos adolescentes, instituindo-lhes marcas e identidades, como tamb??m (re)posicionando-os nos diversos espa??os sociais em que circulam. Essas pr??ticas discursivas v??m interpelando de modo especial...

O escrever e o fotografar de quem aprende : curr??culo, experi??ncia, artes visuais

VIEIRA, Sandra Corr??a
Fonte: Universidade Federal de Pelotas; Educa????o; Programa de P??s-Gradua????o em Educa????o; UFPel; BR Publicador: Universidade Federal de Pelotas; Educa????o; Programa de P??s-Gradua????o em Educa????o; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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Cette dissertation a comme th??matique l??enseignement des Arts Visuels dans l????ducation formelle en remarquant comment les ??l??ves donnent du sens aux exp??riences v??cues dans cette discipline du curriculum. Les proc??dures adopt??es au d??veloppement de la recherche ont ??t?? inspir??es de la cartographie et ont utilis??es l????criture des propres ??l??ves ?? propos de l??Art et de son enseignement et une prise de photographies faite par les ??l??ves eux-m??mes. Le travail analytique a utilis?? quelques ??tudes du champ de la philosophie de la Diff??rence en ayant comme cat??gories les notions de curriculum et de l??exp??rience ?? partir de cette perspective. ?? l??aide des proc??dures, la recherche a pu ??tre d??velopp??e avec une classe de huiti??me, le soir, dans l??ambiance de travail de la rechercheuse. Parmi les r??sultats de la recherche, on discute la place de l??art au sein d??une ??ducation dont les objectifs sont instrumentaux et utilitaires. On discute aussi le temps concernant ?? la discipline au curriculum des ??coles. On a remarqu?? que les ??l??ves poss??dent dans leur quotidien des exp??riences artistiques qui ne sont pas consider??es dans le curriculum des ??coles; Esta disserta????o tem como tem??tica o ensino de Artes Visuais na educa????o formal...

S??ndrome de Burnout: um estudo com professores de Educa????o F??sica das escolas municipais de Pelotas; Burnout Syndrome: a study with Physical Education teachers from the municipal schools of Pelotas

SINOTT, Edilene Cunha
Fonte: Universidade Federal de Pelotas; Educa????o F??sica; Programa de P??s-Gradua????o em Educa????o F??sica; UFPel; BR Publicador: Universidade Federal de Pelotas; Educa????o F??sica; Programa de P??s-Gradua????o em Educa????o F??sica; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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The Burnout Syndrome (BS) is related to the laboral exercise, and occurs mainly in professions which present as a characteristic the intense contact with other people, like in teaching as well. This research aimed to investigate the presence of BS in Physical Education teachers from the municipal schools of Pelotas. The investigation featured as a research of transversal descriptive matters. From a population of 118 teachers, the sample was composed by 94 teachers from the 40 schools of the municipal ring. In the data acquisition, the Maslach Burnout Inventory was utilized for the evaluation of the syndrome and a questionnaire for the socio-demographic characterization, and the context of teaching intervention. The collected data were stored in Excel s spreadsheet, version 2007, and the analyses were carried out by STATA version 12. The data has had normal distribution, and the parametric statistics was performed in the analysis, with a significance level of p<00.5. The Chi-Square Test and The Exact of Fisher were used to verify the difference among the variable which characterize the BS: Emotional Exhaustion (EE), Depersonalization (DE), and Professional Fulfillment (PF). It was testified that 36.2% belonged to the male, and 63.8%...

Efeito do est??mulo ??s rela????es interpessoais na aprendizagem de uma tarefa motora; Effect of stimulation of interpersonal relations in learning a motor task

SANTOS, Leontine Lima dos
Fonte: Universidade Federal de Pelotas; Educa????o F??sica; Programa de P??s-Gradua????o em Educa????o F??sica; UFPel; BR Publicador: Universidade Federal de Pelotas; Educa????o F??sica; Programa de P??s-Gradua????o em Educa????o F??sica; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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The classroom is a perfect micro system, where the teacher interferes in the students training through the manipulation of learning variables and interpersonal relations (SCHILD 1999). The present study is characterized as an experimental study that had the objective of verifying the effect of interpersonal relations on the learning of a motor task in adolescents. The sample was intentionally made of 72 students from a school in Pelotas-RS, divided into two groups: G1 and G2. As treatment of the independent variable, G1 was motivated to interpersonal relations through observation dyads and team action guided by the teacher, while G2 was not. To verify the learning, pre and post tests were performed, using as a tool a Matrix Analysis of Long Jump. The results of the intragroup analysis showed that learning occurred in G1 and G2, with level of significance of p<0,001. The intergroup analysis showed that there was no significant difference between learning scores, allowing concluding that the stimulus to interpersonal relations did not interfere on learning; A sala de aula constitui um microssistema perfeito, onde o professor interfere na forma????o dos seus educandos, atrav??s da manipula????o das vari??veis de aprendizagem e das rela????es interpessoais (SCHILD...

Dor de origem dent??ria e fatores associados: um estudo de base populacional em adultos - Pelotas, RS, 2005.; Toothache prevalence and associated factors: a population-based study in Southern Brazil, 2005.

Bastos, Jo??o Luiz Dornelles
Fonte: Universidade Federal de Pelotas; Medicina; Programa de P??s-Gradua????o em Epidemiologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Medicina; Programa de P??s-Gradua????o em Epidemiologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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The aim of this study was to estimate toothache prevalence and associated factors among adult residents in a Southern Brazilian city, named Pelotas. A cross-sectional population-based study was carried out with individuals aged 20 years and over (n=3353). A multi-stage sampling scheme was adopted, and data collection was performed at participants homes through standardised pre-tested questionnaires. Toothache in the past six months was regarded as the outcome. Socio-economic and demographic data as well as health related behaviours were collected. All analyses were undertaken with a Poisson regression model, following a hierarchical conceptual model. The response rate was 93.5% and toothache prevalence was 17.7% (CI95% 16.0-19.3). Multivariable analyses revealed that toothache was more likely to be reported by those with low educational attainment and low family income. Younger subjects, women and those with black skin colour were also more likely to report toothache. Current smokers and problem drinkers were at increased risk of experiencing toothache in the past six months. The toothache prevalence reported in the present study is not negligible and should stimulate preventive policies and support the planning of local oral health services.; Nos ??ltimos seis meses...

Avalia????o biol??gica de estruturas florais em macieira e sua rela????o com a qualidade do fruto

MADAIL, Rafael Hansen
Fonte: Universidade Federal de Pelotas; Biologia; Programa de P??s-Gradua????o em Fisiologia Vegetal; UFPel; BR Publicador: Universidade Federal de Pelotas; Biologia; Programa de P??s-Gradua????o em Fisiologia Vegetal; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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The apple tree is characterized by the different floral structures. Several researches show a relationship between the production and fruit quality and the original fruiting structure. The aim of this study was to evaluate the relationship between the floral structure and the fruit quality in the cultivars Gala, Fuji and Daiane in the mild climatic conditions in the south of Brazil. Six types of fruiting structures were characterized according to their nature and the bud size: spur of weak bud and spur of vigorous bud, short twig of terminal growth of weak bud and short twig of terminal growth of vigorous bud and long twig of terminal growth of weak spur and vigorous spur. For these different structures, were evaluated characteristics related to the phenological development, like the average dates of each phenological state and the percentage of buds that reached the different phenological phases. Patterns related to the productive potential and the fruit quality were evaluated, like the sprout and fruit set index, bud abortion index and percentage of vegetative buds, leaf area, number of flower per cluster and the length / diameter ratio of the fruits. In Gala and Fuji cultivars, the long twigs had the earlier phenological development and in Daiane...

Efeito do laser de baixa pot??ncia na prolifera????o de fibroblastos pulpares humanos e na atividade das metaloproteinases 2 e 9 in vitro; Effect of low power laser in the human pulp fibroblasts proliferation and in the activity of metalloproteinases 2 and 9 in vitro

CARVALHO, Rodrigo Varella de
Fonte: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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The aim of the present study was to evaluate the gelatinase activity (MMP-2 and 9), and proliferation of human pulp fibroblasts in vitro, after irradiation with a low level diode laser (780nm). The activity was evaluated with zymography and the proliferation rate was obtained through growth curves made in SigmaPlot (8.0), after the counting in Neubauer chamber.in light microscope. Cells used to zimography were cultived in DMEM, 10% fetal bovine serum and incubated at 37??C in atmosphere of 5% CO2 and 95% air, in 100% humidity. Cells used to proliferation rate were cultivated in nutritional deficit (5% SFB). Groups to zymography and cell proliferation were determined: zymography - Z1 = not irradiated, Z2 = 3J/cm2 e Z3 = 6J/cm2, cell proliferation: L1 = not irradiated, L2 = 3J/cm2 e L3 = 6J/cm2. The counting was made after 2, 4 and 6 days. Assays were performed in triplicate. The zymography showed that the MMP-2 activity was major with MMP-9, and, Z2 e Z3 produced a higher activity than MMP-2, when compared to Z1. Results of the zymography and proliferation test were compared by either ANOVA complemented by Tukey??s test. The level of significance was 5% (p<0,05). Statistical analysis demonstrate difference between the groups in different days. The L3 obtained higher proliferation in the finish of the six days. L2 demonstrated higher growth than L1. Irradiated fibroblasts with the low power laser expressed a higher amount of MMP-2 activity and 6J/cm2 produced a higher proliferation rate in the initial days; O objetivo do presente estudo foi avaliar a atividade das gelatinases (MMP-2 e MMP-9)...

Biofilmes gerados a partir da saliva de crian??as sem e com c??rie na primeira inf??ncia: resposta ao desafio cariog??nico e correla????o com os de suas m??es; Biofilms generated from saliva of children without and with early childhood caries: response of cariogenic challenge and correlation with their mothers

Azevedo, Marina Sousa
Fonte: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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Micro-organisms belonging to the resident oral microflora are organized into complex communities, the biofilms, and are the direct cause of the caries process. It is necessary to understand the degree to which biofilm physicochemical and nutrient environments affect the plaque development in individuals differing in their oral environments. In this context, microcosm biofilm models could be used to answer specific questions related to caries. The aim of this study was to test the hypothesis that microcosm biofilms growth from inoculums of caries free children (CF), childrenwith early childhood caries (ECC) and with severe early childhood caries (S-ECC) would provide the same cariogenic response under regular sucrose exposure. Also, microcosm biofilms formed from saliva of children and their mothers considering cariogenic response and microbiota composition were compared. Three children of each group were selected: CF, ECC and S-ECC and their mothers. The mother-child pairs were examined and the dental status was recorded. Saliva samples were collected and screened for microbial composition. Microcosm plaque biofilms were initiated from saliva, growth on bovine enamel discs in artificial saliva enriched with mucin (DMM) for up to 10 days in 24-well microplates under anaerobic conditions. Biofilms were growth with and without sucrose under a semi-dynamic regimen previously tested. DMM was replaced daily and pH measurements were recorded for DMM with sucrose or DMM without sucrose supernatants.On the 10th day...

Citotoxicidade e genotoxicidade do percarbonato de s??dio: compara????o com agentes clareadores comumente utilizados no tratamento de dentes despolpados; Cytotoxicity and genotoxicity of sodium percarbonate: a comparison with bleaching agents commonly used in discolored pulpless teeth

MOR??NIGO, Maria Raquel Fern??ndez
Fonte: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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Aim: To evaluate the cytotoxicity and genotoxicity of sodium percarbonate in comparison with bleaching agents used in discolored pulpless teeth from a mouse fibroblast cell culture. Methodology: The bleaching agents cytotoxicity and genotoxity were evaluated both in their pure form of products as well as in concentration commonly used in clinical practice. Hydrogen peroxide (HP), carbamide peroxide (CP), sodium perborate (SP) and sodium percarbonate (SPC) were diluted in DMEM in series. To evaluate the cytotoxicity, the survival of 3T3 mouse fibroblasts were measured photometrically by using MTT assay after a 24h exposure period. Genotoxicity was indicated by the micronuclei (MN) formation and the modification of the normal cell was analyzed by light microscopy (400x). The statistical analysis was performed by one-way ANOVA, followed by a multiplecomparison Tukey post hoc test (p < 0.05). Results: All tested groups exhibited a dose-dependent cytotoxicity. However, CP has showed a similar cytotoxic effect when compared with DMEM untreated control (UC) group. PH and SPC were significantly more cytotoxic than SP. Genotoxicity test has showed that SPC and SP have presented an intermediate rate of MN frequency over the UC group. The mean rate of MN frequency to HP was higher and statistically more significant than other groups tested. No difference was observed when CP and UC groups were compared. Conclusions: The results suggest that the cytotoxicity of SPC was similar to HP and significantly more cytotoxic than SP and CP. On the other hand...

Investiga????o da presen??a de determinadas esp??cies bacterianas anaer??bias em dentes dec??duos necrosados e avalia????o de duas t??cnicas de instrumenta????o endod??ntica em Odontopediatria; An investigation on the presence of certain anaerobic bacterial species in necrotic primary teeth and an evaluation of two endodontic instrumentation techniques in pediatric dentistry

GOMES, Genara Brum
Fonte: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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Maintaining the integrity and function of primary dentition is one of the main purposes of pediatric dentistry. Bacteria and their products are responsible for the development of endodontic pathologies in primary teeth. This study aimed to identify the presence of specific anaerobic bacteria in infected pulp chambers (PC) and root canals (RC) of necrotic primary teeth by the PCR method and to make an in vivo comparison of the effectiveness between two molar tooth endodontic instrumentation techniques manual and rotary with reference to the time instrumentation and the time to perform the filling. The study consisted in the microbial investigation of primary teeth with pulp necrosis of 15 patients. Samples were collected by using cotton balls (PC) and sterile absorbent paper points (RC). Bacterial DNA was extracted by means of DNA extraction kits. After DNA extraction, PCR reaction was performed through the use of specific primers to detect the occurrence of the following microorganisms: Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, P. gingivalis, Prevotella intermedia, P. nigrescens, P. tannerae, Tanerella forsythia, Treponema denticola and T. socranskii. PCR products were analized by 1% agarose gel stained by red gel and viewed under ultraviolet transillumination. To compare instrumentation techniques...

Soropreval??ncia de Toxoplasma gondii em bubalinos e bovinos que compartilham a mesma ??rea no sul do Rio Grande do Sul; Seroprevalence of Toxoplasma gondii in buffaloes and cattle that share the same area in southern Rio Grande do Sul state, Brazil

SANTOS, Laura Maria Jorge de Faria
Fonte: Universidade Federal de Pelotas; Biologia; Programa de P??s-gradua????o em Parasitologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Biologia; Programa de P??s-gradua????o em Parasitologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
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Toxoplasmosis is a common zoonosis worldwide caused by Toxoplasma gondii, and high infection prevalence in humans and animals. It is usually asymptomatic, however in immunocompromised individuals and in fetuses it may cause severe injury. Wild and domestic felids are the definitive hosts for the parasite, and they shed oocysts in their faeces. Carnivorism, fecal-oral and congenital is the main route of toxoplasmosis transmission. Further studies are needed to investigate the sanity of buffaloes in relation to T. gondii infection, because of the growing demand for buffalo meat and meat products. This work aims to know the prevalence of T. gondii infection in buffaloes and cattle that share the same area in southern Rio Grande do Sul state, and correlate data obtained, including age factor, to identify possible sources of infection. Blood samples were collected by means of jugular venipuncture in 169 buffaloes and 121 beef cattle of different ages, living in a farm in southern Brazil. T. gondii were performed by the indirect fluorescent-antibody (IFA) technique, according to Camargo (1964), using a WAMA Diagnostic?? kit. Positive and negative control serum to T. gondii and conjugated Anti-bovine IgG (whole molecule) FITC produced in rabbits...

Avalia????o radiol??gica da motilidade g??strica de ratos wistar, submetidos a a????o da eletroacupuntura e de extratos de Rosmarinus officinalis; Radiological evaluation of gastric motility of Wistar rats, subjected to the action of electroacupuncture and extract of Rosmarinus officinalis

CARNEVALI, Taiane Rita
Fonte: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR Publicador: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR
Tipo: Dissertação Formato: application/pdf
POR
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The treatment of gastrointestinal disorders using natural methods such as electroacupuncture and phytotherapy, has been deployed worldwide in human and veterinary medicine. Thus, the work was to determine the action of electroacupuncture and the aqueous and ethanol extracts of Rosmarinus officinalis on gastric motility of rats assessed by radiographic examination, in addition to reporting a case of Situs Inversus Totalis in a Wistar albino rat, diagnosed during the experiment. For evaluation of electroacupuncture on gastric motility, we used 24 Wistar rats, divided into four treatments, T1: electroacupuncture point E36 and BP6; T2: electroacupuncture in falses stitches; T3: sterile distilled water and T4: metoclopramide. Five metallic spheres were administered per animal and performed serial radiographs to monitor the gastric emptying time in the different treatments. By analyzing the time of exit the first spheres, the animals of group T1 ha d an average of 3,5h; in T2 the average was greater than 6h; T3 was 5,3h and the T4 observed average of 4,6h. the action of electroacupuncture was comparable to the action of metoclopramide, which is know to increase peristalsis, whereas the response of the Shan group (T2) was close to the negative control group. In the determination of gastric motility with the use of aqueous and ethanol extracts of R. officinalis...

Efeito da Somatotrofina Su??na (pST) sobre o desenvolvimento testicular, idade ?? puberdade e qualidade esperm??tica de machos su??nos; Effect of porcine somatotropin (pST) on male pig testicular development, age to puberty and semen quality

RABASSA, Viviane Rohrig
Fonte: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR Publicador: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR
Tipo: Tese de Doutorado Formato: application/pdf
POR
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The somatotropin (ST), which has its effects mediated by insulin-like growth factor I, has positive effects on gametogenesis and steroidogenesis, and can be used as an alternative treatment for infertile men. Exogenous somatotropin supplementation improves testicular development, gametogenesis and steroidogenesis, anticipating onset of puberty. However, no studies aiming to determine the mechanisms of action of this hormone on testicular function and age at puberty in healthy young boars were performed. Thus the hypothesis of this study were: 1) prepubertal male pigs receiving exogenous ST have accelerated testicular growth due to the mitogenic actions of IGF-I; 2) an increase in gametogenesis and teroidogenesis with consequent antecipation of age at puberty and 3) improvement of semen quality after the establishment of puberty. To confirm this hypothesis four experiments were performed. The Experiment 1 aimed to determine the interval between applications of porcine ST (pST), based on the determination of IGF-I. Experiment 2 aimed to measure the proliferation of testes cells in boars subjected to pST administration, as well as its effect on the metabolism. In Experiment 1, the mean concentration of serum IGF-I for pST Group was higher (P<0.05) than for Control Group...

Controle de Senecio spp. com pastoreio ovino de acordo com a infesta????o e fenologia da planta e a influ??ncia da cobertura vegetal; Sheep grazing for control of Senecio spp. according to infestation level and phenology and the influence of vegetation cover

KARAM, Fernando S??rgio Castilhos
Fonte: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR Publicador: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR
Tipo: Tese de Doutorado Formato: application/pdf
POR
Relevância na Pesquisa
35.46%
The present study yielded a review article on the intoxication by Senecio spp. (seneciosis) and on the control measures for this important cause of death among cattle in the state of Rio Grande do Sul (RS), Brazil. Given that no efficient treatment exists, measures that combine the biological control of the plant and the proper management of agricultural land can reduce economic losses. The study also included two experiments one on the influence of vegetation cover on the germination and emergence of Senecio spp. from seeds in the soil, using three management practices in naturally infested areas: 1) plowing with planting; 2) plowing without planting and 3) slashing and mulching; observed fortnightly for one year. Germination was higher (73.1%) where the land was exposed, without planting. In the three areas, June was the month in which emergence was higher (75.6%). The results reveal the importance of vegetation cover for germination and maintenance of the plant in the environment, in addition to the special care needed at this stage and in this region, when the analyzed species are potentially more toxic. This experiment was presented in a seminar (poster session) and will be replicated in other areas for analysis in different environments and regions. Given that sheep are more resistant to the toxic alkaloids released by Senecio spp. and are natural controls against this plant...

Atividade antibacteriana in vitro e in vivo de Schinus terebinthifolius Raddi no controle da mastite bovina; Atividade antibacteriana in vitro e in vivo de Schinus terebinthifolius Raddi no controle da mastite bovina

FACCIN, ??ngela
Fonte: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR Publicador: Universidade Federal de Pelotas; Veterin??ria; Programa de P??s-Gradua????o em Veterin??ria; UFPel; BR
Tipo: Dissertação Formato: application/pdf
POR
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The scientific interest in herbal medicine has led to the development of research based on the popular practices. This study aimed to evaluate the antibacterial activity of the hydroalcoholic extract and essential oil from the leaves and fruits of Schinus terebinthifolius Raddi at two growth stages against bacteria related to bovine mastitis, besides testing a plant extract with antibacterial action, to be used antisepsis in milking. The thesis is presented in two papers. The first refers to the in vitro antibacterial activity of hydroalcoholic extract and essential oil from leaves and fruits at two growth stages, tested by serial microdilution plates against bovine mastitis bacteria. The results were expressed as Minimal Bactericidal Concentration. All extracts showed antibacterial activity. The second paper shows results for the in vivo experiment, which tested the hydroalcoholic extract of the leaves of the plant before and after dipping for twelve consecutive weeks, was used as control iodine commercial, weekly data were collected regarding clinical mastitis, subclinical and new intramammary infections, and biweekly health data sphincter and skin from the ceiling. None of the indices analyzed apresentraram difference between treatments...