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Produção de nanofibras de celulose por hidrólise enzimática; Cellulose nanofibers produced by enzymatic hydrolysis

Heloisa Tibolla
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 26/03/2014 PT
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A presente dissertação objetivou estudar o potencial da técnica de hidrólise enzimática na produção de nanofibras de celulose (NFCs) a partir da casca de bananas verdes da variedade "Terra" (Musa paradisiaca). Na primeira etapa do trabalho, o farelo da casca da banana foi caracterizado com base em suas propriedades físico-químicas, funcional e estrutural. Na segunda etapa, testou-se combinações de tratamentos (químico, hidrólise enzimática e tratamento mecânico) para isolar nanofibras de celulose. Na terceira etapa do trabalho avaliou-se a influência das condições de processo (pH, temperatura, concentração de enzima e concentração de substrato) na hidrólise enzimática com xilanase. Os experimentos foram realizados empregando-se um planejamento fatorial fracionado 24-1 com três pontos centrais. As NFCs foram caracterizadas quanto ao diâmetro, distribuição do comprimento, potencial zeta, grupos funcionais por FTIR, cristalinidade por difração de raios-X (DRX), concentração de NFCs produzidas e característica morfológica por microscopia eletrônica de transmissão (MET). A quarta e última etapa, foi realizada com o intuito de estudar a adição de mais uma hidrólise enzimática, usando o complexo celulolítico...

Caracterização de genótipos de Musa com base na reação a Radopholus similis e de genótipos contrastantes para resistência com base em marcadores moleculares RAPD

Santos, Jansen Rodrigo Pereira
Fonte: Universidade de Brasília Publicador: Universidade de Brasília
Tipo: Dissertação
PT_BR
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Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Fitopatologia, 2007.; A bananeira é uma ótima hospedeira de vários nematóides importantes, sendo os principais, Radopholus similis e Helicotylenchus multicinctus. Um método eficiente, de baixo custo para o produtor e que tem mostrado grande potencial para o controle de fitonematóides é a resistência genética. Uma etapa importante do melhoramento genético é a avaliação da variabilidade genética da planta hospedeira. Neste trabalho objetivou-se avaliar e caracterizar 26 genótipos de bananeira com relação à resistência ao nematóide cavernícola, Radopholus similis e, caracterizar sete genótipos contrastantes para os fenótipos de resistência e suscetibilidade a R. similis utilizando marcadores moleculares RAPD. As plantas (uma por vaso) foram inoculadas, em de casa de vegetação, com uma suspensão contendo 100 juvenis, machos e fêmeas do nematóide. O delineamento experimental foi inteiramente casualizado com quatro repetições e os genótipos foram avaliados 120 dias após a inoculação. Foram avaliados número de nematóides por grama de raiz, em todo o sistema radicular, no solo e o número total de nematóides. Além destes...

Differential gene expression in ripening banana fruit.

Clendennen, S K; May, G D
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /10/1997 EN
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During banana (Musa acuminata L.) fruit ripening ethylene production triggers a developmental cascade that is accompanied by a massive conversion of starch to sugars, an associated burst of respiratory activity, and an increase in protein synthesis. Differential screening of cDNA libraries representing banana pulp at ripening stages 1 and 3 has led to the isolation of 11 nonredundant groups of differentially expressed mRNAs. Identification of these transcripts by partial sequence analysis indicates that two of the mRNAs encode proteins involved in carbohydrate metabolism, whereas others encode proteins thought to be associated with pathogenesis, senescence, or stress responses in plants. Their relative abundance in the pulp and tissue-specific distribution in greenhouse-grown banana plants were determined by northern-blot analyses. The relative abundance of transcripts encoding starch synthase, granule-bound starch synthase, chitinase, lectin, and a type-2 metallothionein decreased in pulp during ripening. Transcripts encoding endochitinase, beta-1,3-glucanase, a thaumatin-like protein, ascorbate peroxidase, metallothionein, and a putative senescence-related protein increased early in ripening. The elucidation of the molecular events associated with banana ripening will facilitate a better understanding and control of these processes...

Petunia Floral Defensins with Unique Prodomains as Novel Candidates for Development of Fusarium Wilt Resistance in Transgenic Banana Plants

Ghag, Siddhesh B.; Shekhawat, Upendra K. Singh; Ganapathi, Thumballi R.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 22/06/2012 EN
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Antimicrobial peptides are a potent group of defense active molecules that have been utilized in developing resistance against a multitude of plant pathogens. Floral defensins constitute a group of cysteine-rich peptides showing potent growth inhibition of pathogenic filamentous fungi especially Fusarium oxysporum in vitro. Full length genes coding for two Petunia floral defensins, PhDef1 and PhDef2 having unique C- terminal 31 and 27 amino acid long predicted prodomains, were overexpressed in transgenic banana plants using embryogenic cells as explants for Agrobacterium–mediated genetic transformation. High level constitutive expression of these defensins in elite banana cv. Rasthali led to significant resistance against infection of Fusarium oxysporum f. sp. cubense as shown by in vitro and ex vivo bioassay studies. Transgenic banana lines expressing either of the two defensins were clearly less chlorotic and had significantly less infestation and discoloration in the vital corm region of the plant as compared to untransformed controls. Transgenic banana plants expressing high level of full-length PhDef1 and PhDef2 were phenotypically normal and no stunting was observed. In conclusion, our results suggest that high-level constitutive expression of floral defensins having distinctive prodomains is an efficient strategy for development of fungal resistance in economically important fruit crops like banana.

Predicting the Benefits of Banana Bunchy Top Virus Exclusion from Commercial Plantations in Australia

Cook, David C.; Liu, Shuang; Edwards, Jacqueline; Villalta, Oscar N.; Aurambout, Jean-Philippe; Kriticos, Darren J.; Drenth, Andre; De Barro, Paul J.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 07/08/2012 EN
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Benefit cost analysis is a tried and tested analytical framework that can clearly communicate likely net changes in producer welfare from investment decisions to diverse stakeholder audiences. However, in a plant biosecurity context, it is often difficult to predict policy benefits over time due to complex biophysical interactions between invasive species, their hosts, and the environment. In this paper, we demonstrate how a break-even style benefit cost analysis remains highly relevant to biosecurity decision-makers using the example of banana bunchy top virus, a plant pathogen targeted for eradication from banana growing regions of Australia. We develop an analytical approach using a stratified diffusion spread model to simulate the likely benefits of exclusion of this virus from commercial banana plantations over time relative to a nil management scenario in which no surveillance or containment activities take place. Using Monte Carlo simulation to generate a range of possible future incursion scenarios, we predict the exclusion benefits of the disease will avoid Aus$15.9-27.0 million in annual losses for the banana industry. For these exclusion benefits to be reduced to zero would require a bunchy top re-establishment event in commercial banana plantations three years in every four. Sensitivity analysis indicates that exclusion benefits can be greatly enhanced through improvements in disease surveillance and incursion response.

Banana-Associated Microbial Communities in Uganda Are Highly Diverse but Dominated by Enterobacteriaceae

Rossmann, Bettina; Müller, Henry; Smalla, Kornelia; Mpiira, Samuel; Tumuhairwe, John Baptist; Staver, Charles; Berg, Gabriele
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /07/2012 EN
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Bananas are among the most widely consumed foods in the world. In Uganda, the country with the second largest banana production in the world, bananas are the most important staple food. The objective of this study was to analyze banana-associated microorganisms and to select efficient antagonists against fungal pathogens which are responsible for substantial yield losses. We studied the structure and function of microbial communities (endosphere, rhizosphere, and soil) obtained from three different traditional farms in Uganda by cultivation-independent (PCR-SSCP fingerprints of 16S rRNA/ITS genes, pyrosequencing of enterobacterial 16S rRNA gene fragments, quantitative PCR, fluorescence in situ hybridization coupled with confocal laser scanning microscopy, and PCR-based detection of broad-host-range plasmids and sulfonamide resistance genes) and cultivation-dependent methods. The results showed microhabitat-specific microbial communities that were significant across sites and treatments. Furthermore, all microhabitats contained a high number and broad spectrum of indigenous antagonists toward identified fungal pathogens. While bacterial antagonists were found to be enriched in banana plants, fungal antagonists were less abundant and mainly found in soil. The banana stem endosphere was the habitat with the highest bacterial counts (up to 109 gene copy numbers g−1). Here...

Effect of modified atmosphere packaging on quality and shelf life of ‘Robusta’ banana (Musa sp.) stored at low temperature

Kudachikar, V. B.; Kulkarni, S. G.; Prakash, M. N. Keshava
Fonte: Springer-Verlag Publicador: Springer-Verlag
Tipo: Artigo de Revista Científica
EN
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Banana (Musa sp var. ‘Robusta’) stored under active and passive modified atmosphere packaging (MAP) at 12 ± 1°C and 85–90% RH for 2 seasons were evaluated for fruit quality and shelf-life. A steady state of about 8.6 and 8.2% of CO2 and 2.8 and 2.6% of O2 in passive MAP and MAP+GK (Green Keeper) packages, respectively, were established after 3 weeks of storage. Passive MAP and MAP+GK treatments of banana resulted in reduction in physiological loss in weight (PLW) of 0.7 and 0.8% after 5 and 7 weeks of storage, respectively as against 5% PLW in openly kept green banana after 3 weeks. Both MAP and MAP+GK treatments delayed colour, texture, pulp to peel ratio and total soluble solids (TSS) content as compared to openly kept control banana. Results indicated that the shelf life of fruits packed under MAP and MAP+GK could be extended up to 5 and 7 weeks, respectively as compared to 3 weeks for openly kept control fruits. Sensory quality of fully ripe fruits of both passive MAP and MAP+GK treatments, 5 days after ethrel dip was very good. Thus, MAP+GK at 12 ± 1°C and 85–90% RH could be commercially used for long term storage and long distance transportation of banana with maximum shelf-life of 7 weeks.

MusaWRKY71 Overexpression in Banana Plants Leads to Altered Abiotic and Biotic Stress Responses

Shekhawat, Upendra K. S.; Ganapathi, Thumballi R.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 08/10/2013 EN
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WRKY transcription factors are specifically involved in the transcriptional reprogramming following incidence of abiotic or biotic stress on plants. We have previously documented a novel WRKY gene from banana, MusaWRKY71, which was inducible in response to a wide array of abiotic or biotic stress stimuli. The present work details the effects of MusaWRKY71 overexpression in transgenic banana plants. Stable integration and overexpression of MusaWRKY71 in transgenic banana plants was proved by Southern blot analysis and quantitative real time PCR. Transgenic banana plants overexpressing MusaWRKY71 displayed enhanced tolerance towards oxidative and salt stress as indicated by better photosynthesis efficiency (Fv/Fm) and lower membrane damage of the assayed leaves. Further, differential regulation of putative downstream genes of MusaWRKY71 was investigated using real-time RT-PCR expression analysis. Out of a total of 122 genes belonging to WRKY, pathogenesis-related (PR) protein genes, non-expressor of pathogenesis-related genes 1 (NPR1) and chitinase families analyzed, 10 genes (six belonging to WRKY family, three belonging to PR proteins family and one belonging to chitinase family) showed significant differential regulation in MusaWRKY71 overexpressing lines. These results indicate that MusaWRKY71 is an important constituent in the transcriptional reprogramming involved in diverse stress responses in banana.

Physicochemical and Microbial Properties of the Korean Traditional Rice Wine, Makgeolli, Supplemented with Banana during Fermentation

Kim, Eunkyung; Chang, Yoon Hyuk; Ko, Jae Youn; Jeong, Yoonhwa
Fonte: The Korean Society of Food Science and Nutrition Publicador: The Korean Society of Food Science and Nutrition
Tipo: Artigo de Revista Científica
Publicado em /09/2013 EN
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The objective of the present study was to evaluate the physicochemical and microbial properties of the Korean traditional rice wine Makgeolli, supplemented with banana during 6 day fermentation. The alcohol contents of the control and banana Makgeolli were 17.0 and 16.5%, respectively. The pH values decreased while total acidity, total soluble solids, and color values increased throughout the fermentation process. An increase in microorganism counts throughout the 6-day fermentation period was noted in all samples. The major free sugar and organic acid detected in all samples were glucose and succinic acid, respectively. There were 39 volatile compounds detected in the control and banana Makgeolli. The major ester detected was ethyl acetate (20.037 and 22.604% for the control and banana Makgeolli, respectively). The major alcohol compounds detected were 3-methylbutanol (20.933%) and 3-methyl-1-butanol (34.325%) in the control. 2-mtehyl-1-propanol (22.289%) and 3-methyl-1-butanol (39.851%) were the highest alcohol compounds detected in the banana Makgeolli.

Production of bioethanol using agricultural waste: Banana pseudo stem

Ingale, Snehal; Joshi, Sanket J.; Gupte, Akshaya
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica
Publicado em 09/10/2014 EN
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India is amongst the largest banana (Musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars. The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to produce ethanol. Fermentation of cellulosic hydrolysate (4.1 g%) gave maximum ethanol (17.1 g/L) with yield (84%) and productivity (0.024 g%/h) after 72 h. Some critical aspects of fungal pretreatment for saccharification of cellulosic substrate using A. ellipticus and A. fumigatus for ethanol production by S. cerevisiae NCIM 3570 have been explored in this study. It was observed that pretreated banana pseudo stem can be economically utilized as a cheaper substrate for ethanol production.

Small RNA Profiling of Two Important Cultivars of Banana and Overexpression of miRNA156 in Transgenic Banana Plants

Ghag, Siddhesh B.; Shekhawat, Upendra K. S.; Ganapathi, Thumballi R.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 11/05/2015 EN
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Micro RNAs (miRNAs) are a class of non-coding, short RNAs having important roles in regulation of gene expression. Although plant miRNAs have been studied in detail in some model plants, less is known about these miRNAs in important fruit plants like banana. miRNAs have pivotal roles in plant growth and development, and in responses to diverse biotic and abiotic stress stimuli. Here, we have analyzed the small RNA expression profiles of two different economically significant banana cultivars by using high-throughput sequencing technology. We identified a total of 170 and 244 miRNAs in the two libraries respectively derived from cv. Grand Naine and cv. Rasthali leaves. In addition, several cultivar specific microRNAs along with their putative target transcripts were also detected in our studies. To validate our findings regarding the small RNA profiles, we also undertook overexpression of a common microRNA, MusamiRNA156 in transgenic banana plants. The transgenic plants overexpressing the stem-loop sequence derived from MusamiRNA156 gene were stunted in their growth together with peculiar changes in leaf anatomy. These results provide a foundation for further investigations into important physiological and metabolic pathways operational in banana in general and cultivar specific traits in particular.

Comparative Susceptibility of Two Banana Cultivars to Banana Bunchy Top Virus Under Laboratory and Field Environments

Hooks, C.R.R.; Manandhar, R.; Perez, E. P.; Wang, K.-H.; Almeida, R.P.P.
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica Formato: text/html
EN
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Field and laboratory experiments were carried out on the island of Oahu, HI, to compare the susceptibility of the two most commonly grown banana (Musa sp.) cultivars in the state (‘Dwarf Brazilian’ or Santa Catarina [locally known as dwarf apple] and ‘Williams’) to the aphid-borne Banana bunchy top virus (genus Babuvirus, family Nanoviridae, BBTV). Several morphological and physiological features of the two cultivars were monitored to determine whether the banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae), transmits BBTV to both cultivars at a similar rate; and whether after successful inoculation, does each cultivar respond similarly to viral infection. Results from the laboratory experiment showed that a similar percentage of both cultivars were infected with BBTV by aphid vectors (>90% for both cultivars). However, field results showed a significantly lower percentage of dwarf apple (39%) infected with BBTV compared with Williams (79%). We also found that all physiological and morphological features measured (i.e., plant height, leaf area, canopy, chlorophyll level, and moisture content) for both cultivars were impacted similarly by BBTV. The incubation period, or the time between plant infection and initial appearance of disease symptoms...

Stability and esterification of lutein in bread wheat during post harvest storage in comparison with banana.

Tufail Ahmad, Fauziah
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2013
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Lutein is an important micronutrient for humans as well as being the primary contributor to the pale creamy to yellow colour of bread wheat and durum based products but tends to be unstable against heat and UV light. During post harvest storage of bread wheat grain some of the lutein may be converted to mono- and di-fatty acid esters that appear to be more stable forms of lutein. The aims of the work presented in this thesis were: to study the effects of temperature on lutein esterification; to compare the relative stability of free lutein and lutein esters in grain stored under wide temperatures and conditions; to confirm that esterification is an enzymic process; to examine the genetic control mechanisms; to attempt to identify the enzyme and the endogenous substrate source of fatty acids; and finally to compare esterification in wheat grain with the same process in banana fruit tissues. This study utilised a high lutein, ester forming bread wheat, Triticum aestivum L. cv DM5685*B12, a non-ester forming bread wheat cv Haruhikari and a high lutein durum wheat, Triticum durum L cv Kamilaroi, that like many durum cultivars does not form lutein esters. Reverse phase high pressure liquid chromatography (RP-HPLC) was used to quantify the lutein and lutein ester concentrations. Lutein esterification was strongly favoured by low relative humidity (8% RH) and followed a first order reaction rate. The maximum rate of lutein esterification was at ≈80°C...

A Socio-Economic History of the International Banana Trade, 1870-1930

ABBOTT, Roderick
Fonte: Instituto Universitário Europeu Publicador: Instituto Universitário Europeu
Tipo: Trabalho em Andamento Formato: application/pdf; digital
EN
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The genesis, and even more the growth, of the international banana industry is intimately bound up with the development of steamships (from 1850 onwards) and with the spread of railway construction around the world. The coming of steam, which ensured consistent and swifter passage from the Caribbean and Central America to the United States, and later to Europe, meant that bananas could be delivered in good condition rather than rotten, as had happened in earlier days. Later, when refrigerated techniques became available and specialised vessels were built, transport of bananas ceased to be a major problem. Railways were an essential counterpart in the transport chain, being the adaptable way of moving the crop from the plantation to the shipside; and increasingly the profits from banana exports became the preferred method of financing rail construction in Central America. The working paper traces developments from the earliest commercial days, probably in Panama, closely followed by Jamaica, certainly by 1870. This was very much a pioneer industry, with firms set up and joint ventures created, only to collapse as rapidly when a ship or its cargo was lost at sea. At the plantation end it was literally jungle clearance and thousands of workers lost to disease...

Impacto de ingrediente funcional sobre a saúde intestinal; Impact of functional ingredient on intestinal health

Souza, Gabriela Santos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 20/05/2014 PT
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O microbioma intestinal influencia de maneira positiva e/ou negativa o bem estar e o equilíbrio da saúde no organismo humano. Carboidratos não disponíveis, tais como frutanos e amido resistente (encontrado na farinha de banana verde), são ingredientes funcionais que resistem à digestão, estimulam o peristaltismo e o trânsito intestinal, e podem aumentar população de bactérias benéficas. O presente estudo teve como objetivo avaliar o impacto do consumo regular e descontinuado (durante 6 semanas e 3 vezes por semana) de sopas adicionadas de inulina (8 g) ou farinha de banana verde (FBV) (8 g), sobre o funcionamento intestinal em voluntários saudáveis. Para a avaliação do funcionamento intestinal, o questionário semanal Gastrointestinal Symptom Rating Scale foi validado para o idioma português (Brasil), apresentando alfa de Cronbach de 0,82 e correlação intraclasse de 0,83. Além desta ferramenta, também foi utilizado um questionário diário de hábito gastrintestinal, que inclui a Escala de Bristol. Após o consumo das sopas adicionadas de inulina, os voluntários apresentaram redução na consistência das fezes, aumento na frequência de evacuações e na necessidade de evacuar, entretanto foi observado aumento leve de flatulência. O grupo que consumiu sopas adicionadas com FBV apresentou redução na consistência das fezes...

Comportamento reologico dos pures de banana, goiaba, manga e mamão a temperatura ambiente e teste do desempenho de um viscosimetro de tubos

Leoncio Nicanor Otoya Palomino
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em //1971 PT
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Foram estudadas as características reologicas dos purês de quatro frutas tropicais e as características do desempenho de um viscosxmetro de tubos, cujo desenho era uma modificação daquele apresentado por Saravacos. As determinações experimen tais em cada purê foram correlacionadas ao modelo da Lei da Potência , determinando-se os seus parâmetros característicos pelo método dos mínimos quadrados. Na verificação da validade da re gressão linear foi empregado o Teste de Fisher, sendo que os raios de correlação indicaram 95% de probabilidade para os dados do purê de banana e 99% para os dados dos purês de goiaba, manga e mamão* Os parâmetros do comportamento do fluxo n foram menores que a unidade (banana 0 ,492 , goiaba 0,475, manga 0 ,329 e mamão 0,480), indicando desta, maneira que todos eles possuíam comporta mento pseudoplãstico. Os índices de consistência K1 foram 40,91 no purê de banana, goiaba 47,98, manga 24,60 e 12,50 no caso do purê de mamão. Ho referente ao desempenhe do visoosímetro, a análise das fontes de erro indicaram que elas possuíam influência despresível nas determinações experimentais. A análise geral dos erros permitiu estimar que os dados experimentais eram re produzíveis na faixa de + 5%.; The rheological behavior of four tropical fruit purees...

A bananicultura e a transição agroecológica

Caldeira, Maurício Antônio Doro
Fonte: Florianópolis, SC Publicador: Florianópolis, SC
Tipo: Dissertação de Mestrado Formato: 106 f.| il., tabs.
POR
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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-Graduação em Agroecossistemas.; O trabalho foi desenvolvido em Dom Pedro de Alcântara (RS), onde 90% da população habitam a área rural. A estrutura fundiária se caracteriza por pequenas propriedades, com 88% menores que 10 hectares. A população é formada por agricultores familiares, que têm como fonte de trabalho e renda a bananicultura. O município localiza-se em área de domínio da "Mata Atlântica". O cultivo da banana começou a se desenvolver no município a partir de 1950, com intensificação sistema produtivo. A tecnologia exigia dos agricultores maior aporte de recursos financeiros para a manutenção do cultivo de banana. Por outro ângulo, em Dom Pedro de Alcântara, a cobertura original da Floresta Tropical Atlântica era de 100%. Hoje, permanecem 15% da cobertura original, na forma de áreas remanescentes florestais. O aproveitamento de recursos florestais ainda existe nas propriedades, traduzindo uma importante relação econômica, social e cultural com a floresta. Frente a isso, foi importante analisar a agricultura praticada em áreas próximas a remanescentes florestais, em Dom Pedro de Alcântara...

Atraso do amadurecimento de banana 'Maçã' pelo 1-MCP, aplicado previamente à refrigeração

Almeida,Gustavo Costa; Vilas Boas,Eduardo Valério de Barros; Rodrigues,Luiz José; Paula,Nélio Ranieli Ferreira de
Fonte: Sociedade Brasileira de Fruticultura Publicador: Sociedade Brasileira de Fruticultura
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2006 PT
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O objetivo deste trabalho foi avaliar o efeito de diferentes períodos de exposição da banana 'Maçã' a 50 ppb de 1-MCP (0; 3; 6; 9; 12 e 24 horas) sobre sua vida pós-colheita e qualidade. Após exposição ao 1-MCP, os frutos verde-maturos foram armazenados por 30 dias em câmaras com temperatura de 13ºC ± 0,5 e umidade relativa de 95%. Em seguida, as bananas foram armazenadas à temperatura de 20ºC ± 1, até amarelecimento completo da casca. A exposição de banana 'Maçã' a 50 ppb de 1-MCP, por 9 horas, retardou em 7 dias o seu amadurecimento, em comparação a frutos não expostos ao 1-MCP, após 30 dias de armazenamento refrigerado (13ºC), sem prejuízos à sua aparência e composição química. A exposição de banana 'Maçã' a 50 ppb de 1-MCP, por 3 e 6 horas, não estendeu sua vida pós-colheita, tampouco alterou sua composição química. Embora a exposição de banana 'Maçã' a 50 ppb de 1-MCP, por 12 e 24 horas, tenha retardado o seu amadurecimento, promoveu alterações indesejáveis na casca do fruto. Logo, a aplicação de 50 ppb de 1-MCP, por 9 horas, antes da refrigeração, constitui-se numa alternativa viável para prolongar o período de comercialização da banana.

Climatização de frutos de bananeiras com características de resistência à sigatoka-negra

Saes Junior, Luiz Alberto
Fonte: Universidade Federal do Paraná Publicador: Universidade Federal do Paraná
Tipo: Dissertação Formato: 74f. : il., tabs., grafs., color.; application/pdf
PORTUGUêS
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Orientadora : Profª. Drª. Francine Lorena Cuquel; Co-orientador : Dr. Erval Rafael Damatto Junior; Dissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências Agrárias, Programa de Pós-Graduação em Agronomia. Defesa: Curitiba, 10/06/2014; Inclui referências; Área de concentração: Produção vegetal; Resumo: Mundialmente a banana é o fruto de maior consumo in natura, e por isso a bananeira é cultivada em uma grande área e em diversos países no mundo todo. Bananas do subgrupo Cavendish são preferidas pelo mercado externo e do subgrupo Prata pelo brasileiro. Entretanto a produção da banana sofreu considerável declínio devido à presença de uma doença fúngica, a Sigatoka negra, que reduz a produção e afeta negativamente a qualidade dos frutos. Atualmente, um dos grandes desafios da pesquisa é a introdução de cultivares com características de cultivo desejadas, como resistência às doenças e características sensoriais semelhantes aos frutos com aceitação no mercado. O presente trabalho objetivou estudar os parâmetros adequados para a climatização dos frutos das cultivares ‘Nanicão IAC 2001’ e a ‘PA 94-01’, avaliar suas qualidades físicas e químicas e compará-las com as mesmas dos frutos padrão de comercialização. Para tanto foram desenvolvidos um experimento com a cultivar Nanicão IAC 2001 (subgrupo Cavendish)...

Plant regeneration from long-term callus culture of aaa-group dessert banana.

CONCEICAO, A. da S.; MATSUMOTO, K.; BAKRY, F.; BERND-SOUZA, R.B.
Fonte: Pesquisa Agropecuaria Brasileira, Brasilia, v.33, n.8, p.1291-96, ago.1998. Publicador: Pesquisa Agropecuaria Brasileira, Brasilia, v.33, n.8, p.1291-96, ago.1998.
Tipo: Artigo em periódico indexado (ALICE)
EN
Relevância na Pesquisa
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The banana plant is one of the most widely cultivated crops in the world. However, banana breeding has been a slow process, due to the low seed set and low germination rates. Selection of useful somaclonal variations and genetic transformation in cells or calluses are promising techniques to accelerate the breeding process. Therefore, callus culture was carried out, aiming the establishment of one protocol for plant regeneration, to be used in banana breeding program. Leaf sheath disks of 'Nanicao' banana (Musa sp., AAA group, Cavendish subgroup) were cultured on a Murashige and Skoog (MS) basal medium supplemented with activated charcoal (0.2%), MES (2 [N-morpholino] ethanesulfonic acid) (15.3 mM) arginine (300 mM), Picloram (414 M) and 2iP (2-isopenteny adenine) (492 M). Globular calluses developed on the leaf tissue were subcultured in the same medium, acquiring a friable and translucid appearance after one and a half year of culture. The friable calluses were transferred to the medium without growth regulators and arginine, and supplemented with casein hydrolysate (0.05%), where they formed embryo-like structures after transference to light. From these structures, shoots with roots were obtained and plantlets developed. The plant regeneration protocol shown here may be useful to banana breeding via somaclonal variation.; 1998