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Abrindo caixas de memórias: escritas inventivas em pesquisas sobre educação e meio ambiente

Sezerino, Manoela
Fonte: Florianópolis, SC. Publicador: Florianópolis, SC.
Tipo: Trabalho de Conclusão de Curso Formato: 74
PT_BR
Relevância na Pesquisa
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TCC(graduação) - Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Biologia.; Eis aqui, enfim, o meu Trabalho de Conclusão de Curso (TCC). Apresento a você leitor(a) uma pesquisa que desvenda uma parte da história do curso de Ciências Biológicas na UFSC e revela memórias de biólogos em formação, quando estes começam a se pensar educadores ambientais. “Abrindo caixas de memórias: escritas inventivas em pesquisas sobre educação e meio ambiente” mostra quando os trabalhos em educação começaram a ser produzidos no Centro de Ciências Biológicas e a multiplicidade de escritas e estruturas neles encontrados – trabalhos redigidos em primeira pessoa, marcados fortemente pela subjetividade; trabalhos redigidos em terceira pessoa, totalmente impessoais; trabalhos com e sem referência à trajetória pessoal ou acadêmica; diferentes formas de narrar esta trajetória; trabalhos que seguem o padrão e a estrutura de pesquisas no bacharelado; trabalhos que seguem um padrão e estrutura próprios, etc. O primeiro capítulo do meu TCC apresenta a parte metodológica da pesquisa. Nele eu conto do momento em que procurei a orientação, passando pelos caminhos e descaminhos que me levaram a escolher o tema e desenvolver este trabalho...

Terapia cognitivo-comportamental: A preparação do paciente com transtorno de pânico para as exposições agorafóbicas interoceptivas e in vivo

King, Anna Lucia Spear; Psicóloga, Mestre em Saúde Mental, Doutoranda em Saúde Mental e Pesquisadora do Laboratório de Pânico e Respiração (LABPR) do Instituto de Psiquiatria (IPUB) da Universidade Federal do Rio de Janeiro (UFRJ). Instituto Nacion
Fonte: ISPA - Instituto Universitário Publicador: ISPA - Instituto Universitário
Tipo: Artigo de Revista Científica Formato: application/pdf
Publicado em 23/11/2012 POR
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Objetivo: O estudo com terapia cognitivo-comportamental (TCC) procurou demonstrar a preparaçãodo paciente com transtorno do pânico para exposições agorafóbicas. Foram usadas as técnicas dasexposições interoceptivas, às próprias sensações corporais, e in vivo, a locais ou situações temidas.Metodologia: 50 indivíduos, divididos em dois grupos de 25 participantes cada um. O grupo 1 realizou 10 sessões de TCC semanais e individuais com uma hora de duração e fez uso de medicamentos e o grupo 2, controle, usou apenas medicação sem TCC. A medicação prescrita consistiu em antidepressivos tricíclicos e inibidores seletivos de recaptação da serotonina. Foram aplicados instrumentos de avaliação no início e ao fim das intervenções médica e psicológica. Resultados: Observamos uma diferença significativa entre a avaliação inicial e final no grupo 1, como: redução nos ataques de pânico, ansiedade antecipatória, esquiva agorafobia e medo das sensações corporais. Na escala de avaliação global do funcionamento, um aumento do bem-estar global de 60.8% a 72.5% entre pacientes do grupo 1 com terapia, diferentemente do grupo 2 sem terapia. Conclusões: As técnicas de TCC para as exposições foram consideradas essenciais na preparação dos pacientes com transtorno de pânico...

Potenciais biomarcadores da terapia cognitivo-comportamental para o transtorno de estresse pós-traumático: uma revisão sistemática

Gonçalves,Raquel; Lages,Ana Cristina; Rodrigues,Helga; Pedrozo,Ana Lúcia; Coutinho,Evandro Silva Freire; Neylan,Tom; Figueira,Ivan; Ventura,Paula
Fonte: Faculdade de Medicina da Universidade de São Paulo Publicador: Faculdade de Medicina da Universidade de São Paulo
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2011 PT
Relevância na Pesquisa
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CONTEXTO: O estabelecimento de biomarcadores relacionados à terapia cognitivo-comportamental (TCC) é uma maneira de consolidar a eficácia do tratamento de forma mais objetiva, sendo crítico para o avanço desse campo de pesquisa. OBJETIVOS: Foi realizada uma revisão sistemática de estudos que: 1) utilizaram parâmetros biológicos para verificar a eficácia da TCC no tratamento do transtorno de estresse pós-traumático (TEPT); e 2) utilizaram esses parâmetros como preditores de resposta à CBT. MÉTODOS: Foram conduzidas buscas eletrônicas nas bases PubMed/Medline, ISI/Thompson Reuters e Pilot. RESULTADOS: Doze artigos foram selecionados para esta revisão, sendo quatro deles estudos sobre predição de resposta ao tratamento. Foi observada relação entre a eficácia da TCC e modificações nesses parâmetros, sendo a resposta da frequência cardíaca à evocação de sintomas o parâmetro mais utilizado até o presente momento. Sua redução está associada à melhora nos sintomas de TEPT. Potenciais biomarcadores de predição de resposta encontrados incluem 5α-redutase, ativação da amígdala, ativação e volume do córtex cingulado anterior e frequência cardíaca. CONCLUSÃO: Apesar da escassez de estudos e das limitações metodológicas neles observadas...

Efeitos do Tai Chi Chuan na força dos músculos extensores dos joelhos e no equilíbrio em idosas

Pereira,MM; Oliveira,RJ; Silva,MAF; Souza,LHR; Vianna,LG
Fonte: Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia Publicador: Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2008 PT
Relevância na Pesquisa
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CONTEXTUALIZAÇÃO: Alguns estudos têm indicado que o Tai Chi Chuan (TCC) é capaz de melhorar o condicionamento físico, a força muscular e o equilíbrio entre os praticantes idosos, prevenindo quedas, fraturas e dependência física. OBJETIVO: Verificar os efeitos do TCC no equilíbrio (EQ) e na força dos músculos extensores dos joelhos (F) em mulheres idosas. MATERIAIS E MÉTODOS: Participaram do estudo 77 mulheres saudáveis, não praticantes de atividade física orientada. No Grupo Experimental (G1) foram incluídas 38 voluntárias (68 ± 5 anos) e no Grupo Controle (G2), 39 voluntárias (69 ± 7 anos). O G1 praticou o TCC estilo Yang de 24 movimentos durante 12 semanas, três vezes por semana, com duração de 50 minutos. O G2 não realizou atividades físicas orientadas. A força foi mensurada pelo teste de 1-RM na cadeira extensora e o equilíbrio foi avaliado utilizando o teste de apoio unipodal com os olhos fechados. Na análise estatística, utilizou-se teste de normalidade, split-plot análise de variância (ANOVA) e correlação de Pearson. RESULTADOS: O Grupo Experimental apresentou incrementos de 17,83% na F e 26,10% no EQ. O Grupo Controle não apresentou alteração significativa em nenhuma variável. Não foi observada correlação significativa entre estas duas variáveis no G1 (r= 0...

Efeito da prática do Tai Chi Chuan sobre a resistência aeróbia de idosas sedentárias

Chao,Cheng Hsin Nery; Costa,Eduardo Caldas; Elsangedy,Hassan Mohamed; Savir,Paulo André Holanda; Alves,Emanuel Augusto; Okano,Alexandre Hideki
Fonte: Universidade do Estado do Rio Janeiro Publicador: Universidade do Estado do Rio Janeiro
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2012 PT
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OBJETIVO: Investigar o efeito da prática do Tai Chi Chuan (TCC) sobre a resistência aeróbia de idosas sedentárias. Métodos: Fizeram parte do estudo 11 idosas sedentárias (67,9 ± 6,8 anos; 25,9 ± 2,8 kg/m²), as quais foram submetidas aos testes de marcha estacionária de dois minutos e teste de caminhada de seis minutos pré e pós-intervenção de 12 semanas de TCC. RESULTADOS: Houve aumento da resistência aeróbia das idosas analisadas após 12 semanas de prática de TCC, demonstrado tanto por meio do teste de caminhada de seis minutos (510 ± 64 vs. 536 ± 63 metros; p = 0,006) quanto no teste de marcha estacionária de dois minutos (83 ± 20 vs. 110 ± 19 execuções; p = 0,001). CONCLUSÃO: De acordo com os resultados obtidos, é possível concluir que a prática do TCC é capaz de promover aumento da resistência aeróbia de idosas sedentárias.

Percepção subjetiva do esforço, resposta afetiva e hipotensão pós-exercício em sessão de Tai Chi Chuan

Chao,Cheng Hsin Nery; Okano,Alexandre Hideki; Savir,Paulo André Holanda; Alves,Emanuel Augusto; Elsangedy,Hassan Mohamed; Cyrino,Edilson Serpeloni; Farias Junior,Luiz Fernando de; Costa,Eduardo Caldas
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2013 PT
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O objetivo do estudo foi analisar a percepção subjetiva do esforço (PSE), resposta afetiva (RA) e pressão arterial pós-exercício em sessão aguda de Tai Chi Chuan (TCC). Vinte e quatro mulheres (47-79 anos; 9 normotensas e 15 hipertensas) foram submetidas a uma sessão de TCC e uma sessão controle (delineamento cruzado). A PSE foi avaliada pela escala de Borg (CR10), a RA pela escala de valência afetiva (+5/-5) e a pressão arterial (PA) pelo método oscilométrico (5, 10, 25, 20, 25 e 30 min pós-sessão). Redução significante da PA sistólica foi identificada aos 20 e 25 min após a sessão de TCC somente nas hipertensas. Os valores de RA e PSE foram +2,8 ± 1,9 e 3,5 ± 1,2, respectivamente. Os resultados sugerem que uma sessão de TCC de intensidade moderada foi prazerosa para as voluntárias (hipertensas e normotensas), embora tenha induzido discreto efeito hipotensor agudo na PA sistólica somente das hipertensas.

Characterization of Human CD4+ T-Cell Clones Recognizing Conserved and Variable Epitopes of the Lassa Virus Nucleoprotein

ter Meulen, Jan; Badusche, Marlis; Kuhnt, Kristiane; Doetze, Andrea; Satoguina, Judith; Marti, Thomas; Loeliger, Cornelius; Koulemou, Kekoura; Koivogui, Lamine; Schmitz, Herbert; Fleischer, Bernhard; Hoerauf, Achim
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /03/2000 EN
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T cells must play the major role in controlling acute human Lassa virus infection, because patients recover from acute Lassa fever in the absence of a measurable neutralizing antibody response. T cells alone seem to protect animals from a lethal Lassa virus challenge, because after experimental vaccination no neutralizing antibodies are detectable. In order to study human T-cell reactivity to single Lassa virus proteins, the nucleoprotein (NP) of Lassa virus, strain Josiah, was cloned, expressed in Escherichia coli, and affinity purified. Peripheral blood mononuclear cells (PBMC) obtained from 8 of 13 healthy, Lassa virus antibody-positive individuals living in the Republic of Guinea, western Africa, were found to proliferate in response to the recombinant protein (proliferation index ≥10). PBMC obtained from one individual with a particularly high proliferative response were used to generate 50 NP-specific T-cell clones (TCC). For six of these the epitopes were mapped with overlapping synthetic peptides derived from the sequence of the NP. These CD4+ TCC displayed high specific proliferation and produced mainly gamma interferon upon stimulation with NP. Because variation of up to 15% in the amino acid sequences of the structural proteins of naturally occurring Lassa virus variants has been observed...

Terminal complement complexes and C1/C1 inhibitor complexes in rheumatoid arthritis and other arthritic conditions.

Oleesky, D A; Daniels, R H; Williams, B D; Amos, N; Morgan, B P
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1991 EN
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Terminal complement complex (TCC) and C1r-C1s-C1 inhibitor complex (C1/C1 INH) concentrations were measured in plasma and synovial fluid from patients with arthritis and related to other measures of disease activity. Both TCC and C1/C1 INH concentrations were significantly increased in patients with rheumatoid arthritis (RA) compared with patients with osteoarthritis (plasma and synovial fluid, P less than 0.05) and normal subjects (plasma only, P less than 0.001). In the patients with RA, there was no correlation between plasma or synovial fluid TCC concentrations and IgM rheumatoid factor, immune complex or C1/C1 INH levels. However, in 10 patients with seronegative RA, C1/C1 INH and immune complex levels correlated significantly in synovial fluid (r = 0.69, P less than 0.05) although not in plasma (r = 0.52). Plasma and synovial fluid TCC and C1/C1 INH concentrations did not differ in rheumatoid patients with severe compared with mild joint disease (categorized by the Ritchie score). These results confirm a role for complement activation in RA but suggest that several mechanisms are involved in its pathogenesis.

Spontaneous production of various cytokines except IL-4 from CD4+ T cells in the affected organs of sarcoidosis patients.

Hoshino, T; Itoh, K; Gouhara, R; Yamada, A; Tanaka, Y; Ichikawa, Y; Azuma, M; Mochizuki, M; Oizumi, K
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1995 EN
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We investigated surface antigens and spontaneous cytokine production of T cells from bronchoalveolar lavage fluid (BALF) and aqueous humor (AH) from pulmonary sarcoidosis patients for a better understanding of the role of T cells in granuloma formation. The levels of CD3, CD11b, and CD28 antigen expression on freshly isolated T cells in the BALF of patients were significantly lower than those in peripheral blood lymphocytes (PBL) of either sarcoidosis patients or healthy donors (HD). In contrast, the levels of CD80 (B7/B7-1) and CD86 (B70/B7-2) antigen expression were significantly higher on these T cells and alveolar macrophages in the BALF of patients. Fifty-three T cell clones (TCC) established from the BALF and AH of the three sarcoidosis patients displayed primarily either CD4+ CD11b+ CD28+ or CD4+ CD11b- CD28- phenotypes. Most (61-90%) of these TCC spontaneously produced greater amounts of IL-1 alpha, IL-10, tumour necrosis factor (TNF), and granulocyte-macrophage colony-stimulating factor (GM-CSF) than did TCC from the PBL from sarcoidosis patients or HD (P < 0.05). Interferon-gamma (IFN-gamma), IL-6, and IL-2, but not IL-4, were also produced by 40-48% of these TCC. These results suggest that CD4+ T cells of the affected organs of sarcoidosis patients are activated and involved in the immunopathogenesis of sarcoidosis through production of various cytokines.

Clinical significance of DNA ploidy and S-phase fraction and their relation to p53 protein, c-erbB-2 protein and HCG in operable muscle-invasive bladder cancer.

Fosså, S. D.; Berner, A. A.; Jacobsen, A. B.; Waehre, H.; Kvarstein, B.; Urnes, T.; Ogreid, P.; Johansen, T. E.; Silde, J.; Nesland, J. M.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1993 EN
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DNA ploidy and S-phase fraction (SPF), determined by flow cytometry were studied in 118 patients with muscle-invasive transitional cell carcinoma (TCC) of the urinary bladder, scheduled for cystectomy after pre-operative radiotherapy (20 Gy/1 week) with or without systemic cisplatin-based neo-adjuvant chemotherapy. The correlation between these parameters and immunohistochemically demonstrated p53, c-erbB-2 and HCG was also investigated. There were 16 DNA diploid and 102 DNA non-diploid tumours. DNA ploidy was not related to the T (all 118 patients) or pN (58 patients) category, occurrence of stage reduction or cancer-related 5 years survival. Patients with high SPF tumours tended, however, to have a better prognosis than those with low SPF TCC reaching the level of significance (P < 0.05) for those patients who had high SPF tumours and received neo-adjuvant chemotherapy. Fifty-one of the tumours were p53 positive. p53 positive tumours were significantly more often found in TCC with low SPFs than in those with high SPFs. Respectively 12 and 9% of the tumours were HCG and c-erbB-2 positive, without correlation to DNA ploidy or SPF. We conclude that DNA ploidy does not represent a prognostic parameter in muscle-invasive operable bladder carcinomas. A high SPF...

Alterations in expression of the multidrug resistance-associated protein (MRP) gene in high-grade transitional cell carcinoma of the bladder.

Clifford, S. C.; Neal, D. E.; Lunec, J.
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em /03/1996 EN
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Expression of the MRP gene has been demonstrated in vitro to be a casual factor in non-P-glycoprotein-mediated multidrug resistance, and is implicated in resistance to a number of the chemotherapeutic agents currently used in the treatment of high-grade transitional cell carcinoma (TCC) of the bladder (doxorubicin, epirubicin and vinblastine). Using a sensitive RT-PCR-based technique, we have quantified MRP mRNA levels in a series of untreated TCC (n=24), normal bladder (n=5) and control tissue and cell line samples. MRP mRNA was widely expressed and detectable in all samples analysed, with considerable (up to 190-fold) variation observed between individual tumour samples. MRP mRNA levels found in TCC samples were lower than those determined for normal peripheral mononucleocyte (2.3-fold) and testis (4.1-fold) samples, previously reported to be high-expressing tissues, and varied over a similar range to that observed in normal bladder samples. Results indicate that MRP mRNA levels in a greater proportion of high-grade (G3) bladder tumours (55%, 6/11) are significantly reduced (P=0.018) compared with low- and moderate-grade (G1/2) bladder tumours (8%, 1/13), and suggest that MRP mRNA levels frequently become reduced as a consequence of tumour progression to advanced...

Is chromosome 9 loss a marker of disease recurrence in transitional cell carcinoma of the urinary bladder?

Bartlett, J. M.; Watters, A. D.; Ballantyne, S. A.; Going, J. J.; Grigor, K. M.; Cooke, T. G.
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em /06/1998 EN
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Investigation of transitional cell carcinoma of the urinary bladder (TCC) patients classified by recurrence and/or progression has demonstrated that loss of chromosome 9, as detected by FISH analysis of the pericentromeric classical satellite marker at 9q12, occurs early. A total of 105 TCCs from 53 patients were analysed in situ by two independent observers for loss of chromosome 9 using quantitative fluorescence in situ hybridization (FISH). All 53 primary tumours were evaluated for chromosomes 9, 7 and 17. Normal ranges for chromosomal copy number were defined for normal skin epidermis and bladder epithelium. Values for chromosome 9 copy number outwith the range 1.51-2.10 (mean +/- 3 x s.d. of normal values) were significantly abnormal. Twenty-five TCCs were detected with consistent monosomic scores. Of 89 TCCs, in which multiple tumour areas were analysed, 85 tumours (96%) demonstrated the same chromosome 9 copy number in all areas (2-6) analysed; only three tumours demonstrated heterogeneity for this locus. A total of 36% (12 out of 33) of patients with subsequent disease recurrence demonstrated loss of chromosome 9 in their primary and all subsequent TCCs analysed. Only a single patient (n = 20) with non-recurrent TCC showed loss of chromosome 9 (P = 0.0085). Of 53 primary tumours...

Destabilization of chromosome 9 in transitional cell carcinoma of the urinary bladder

Kimura, F; Florl, A R; Seifert, H-H; Louhelainen, J; Maas, S; Knowles, M A; Schulz, W A
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em /12/2001 EN
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The most frequent genetic alteration in transitional cell carcinoma of the urinary bladder (TCC) is loss of chromosome 9 which targets CDKN2A on 9p. The targets on 9q are not confirmed. Here, 81 advanced TCC specimens were investigated for loss of heterozygosity (LOH) and homozygous deletions (HD) on chromosome 9q using multiplex analysis of microsatellite markers. 41/81 tumours (51%) showed LOH on 9q, with LOH at all markers in 33 cases. Eight partial losses involved three regions in 9q12, 9q22.3, and 9q33– 9q34. No mutations were identified in the candidate tumour suppressor gene DBCCR1 in three tumours showing restricted LOH at 9q32-33. 22% of the specimens had HD at CDKN2A, but no HD was found on 9q. Two tumours had lost 9p only and five 9q only. 9q LOH was not related to tumour grade or stage and present or absent with equal frequency in recurrent TCC. LOH on 9q correlated with the extent of genome-wide hypomethylation (P < 0.0001) which extended into satellite sequences located in 9q12 juxtacentromeric heterochromatin. While the high frequency of chromosome 9q loss in TCC may reflect destabilization of the chromosome related to hypomethylation of repetitive DNA, the data are compatible with the existence of tumour suppressor genes on this chromosome arm. http://www.bjcancer.com © 2001 Cancer Research Campaign

Impact of Caveolin-1 Expression on the Prognosis of Transitional Cell Carcinoma of the Upper Urinary Tract

Cho, Dae Sung; Yim, Hyunee; Cho, Kang Su; Hong, Sung Joon; Cho, Nam Hoon; Kim, Sun Il; Ahn, Hyun Soo; Kim, Se Joong
Fonte: The Korean Academy of Medical Sciences Publicador: The Korean Academy of Medical Sciences
Tipo: Artigo de Revista Científica
EN
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This study aimed to investigate the relationship of caveolin-1 expression with prognosis in patients with transitional cell carcinoma of the upper urinary tract (TCC-UUT). Formalin-fixed, paraffin-embedded tissue sections of TCC-UUT from 98 patients, who had undergone radical nephroureterectomy, were stained immunohistochemically using antibodies against caveolin-1. The expression pattern of caveolin-1 was compared with the clinicopathological variables. The caveolin-1 expression was significantly correlated with T stage (p<0.001) and grade (p=0.036). The survival rate of patients with caveolin-1 positive tumors was significantly lower than that of patients with caveolin-1 negative tumors (p<0.0001). The univariate analyses identified T stage, grade, and caveolin-1 expression as significant prognostic factors for cancer-specific survival, whereas the multivariate analyses indicated that T stage and caveolin-1 expression were independent prognostic factors. These results show that the increased expression of caveolin-1 is associated with tumor progression and poor prognosis in TCC-UUT, suggesting that caveolin-1 may play an important role in the progression of TCC-UUT.

Prognostic Impact of Peripelvic Fat Invasion in pT3 Renal Pelvic Transitional Cell Carcinoma

Cho, Kang Su; Cho, Nam Hoon; Park, Sung Yul; Cho, Sung Yong; Choi, Young Deuk; Chung, Byung Ha; Yang, Seung Choul; Hong, Sung Joon
Fonte: The Korean Academy of Medical Sciences Publicador: The Korean Academy of Medical Sciences
Tipo: Artigo de Revista Científica
EN
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Renal pelvic transitional cell carcinoma (TCC), which invades beyond muscularis into peripelvic fat or the renal parenchyma, is diagnosed as stage pT3 despite its structural complexity. We evaluated the prognostic impact of peripelvic fat invasion in pT3 renal pelvic TCC. Between 1986 and 2004, the medical records on 128 patients who were surgically treated for renal pelvic TCC were retrospectively reviewed. Sixty patients with pT3 disease were eligible for the main analysis. The prognostic impact of various clinicopathological factors was analyzed using univariate and multivariate analyses. On univariate analysis, sex, age, concomitant bladder tumors, concomitant ureter tumors, lymphadenectomy, adjuvant chemotherapy, tumor grade, multiplicity, renal parenchymal invasion, and carcinoma in situ did not influence the disease-specific survival (p>0.05). By contrast, peripelvic fat invasion, lymph node invasion, and lymphovascular invasion were each significantly associated with disease-specific survival (p<0.05). Multivariate analysis showed that peripelvic fat invasion (p=0.012) and lymph node invasion (p=0.004) were independent prognostic factors. In conclusion, peripelvic fat invasion is a strong prognostic factor in pT3 renal pelvic TCC. Thus...

Metastasis to the ovaries from transitional cell carcinoma of the bladder and renal pelvis: a report of two cases

Lee, Maria; Jung, Yong Wook; Kim, Sang Wun; Kim, Sung Hoon; Kim, Young Tae
Fonte: Korean Society of Gynecologic Oncology and Colposcopy Publicador: Korean Society of Gynecologic Oncology and Colposcopy
Tipo: Artigo de Revista Científica
EN
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Ovarian metastases from a primary urinary tract carcinoma are extremely rare. This can be difficult to distinguish from transitional cell carcinomas (TCC) of ovarian origin because of histologic similarity. A 65-year-old woman who was diagnosed with renal pelvis TCC 4 months prior was referred for evaluation of a left ovarian mass. A 47-year-old woman who underwent radical cystectomy due to bladder TCC 1 year ago was referred because of a right ovarian mass. Both patients underwent a bilateral salpingo-oophorectomy. The tumor cells had morphology identical to those of the primary urinary tract tumors. Gynecologic oncologists should consider metastatic TCC of the ovary from urinary tract origin, as well as breast, and gastrointestinal tract origins.

Novel SPG11 mutations in Asian kindreds and disruption of spatacsin function in the zebrafish

Southgate, Laura; Dafou, Dimitra; Hoyle, Jacqueline; Li, Nan; Kinning, Esther; Critchley, Peter; Németh, Andrea H.; Talbot, Kevin; Bindu, Parayil S.; Sinha, Sanjib; Taly, Arun B.; Raghavendra, Seetharam; Müller, Ferenc; Maher, Eamonn R.; Trembath, Richa
Fonte: Springer-Verlag Publicador: Springer-Verlag
Tipo: Artigo de Revista Científica
EN
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Autosomal recessive hereditary spastic paraplegia with thin corpus callosum (HSP-TCC) maps to the SPG11 locus in the majority of cases. Mutations in the KIAA1840 gene, encoding spatacsin, have been shown to underlie SPG11-linked HSP-TCC. The aim of this study was to perform candidate gene analysis in HSP-TCC subjects from Asian families and to characterize disruption of spatacsin function during zebrafish development. Homozygosity mapping and direct sequencing were used to assess the ACCPN, SPG11, and SPG21 loci in four inbred kindreds originating from the Indian subcontinent. Four novel homozygous SPG11 mutations (c.442+1G>A, c.2146C>T, c.3602_3603delAT, and c.4846C>T) were identified, predicting a loss of spatacsin function in each case. To investigate the role of spatacsin during development, we additionally ascertained the complete zebrafish spg11 ortholog by reverse transcriptase PCR and 5′ RACE. Analysis of transcript expression through whole-mount in situ hybridization demonstrated ubiquitous distribution, with highest levels detected in the brain. Morpholino antisense oligonucleotide injection was used to knock down spatacsin function in zebrafish embryos. Examination of spg11 morphant embryos revealed a range of developmental defects and CNS abnormalities...

Monitoring of the upper urinary tract in patients with bladder cancer

Ayyathurai, Rajinikanth; Soloway, Mark S.
Fonte: Medknow Publications Publicador: Medknow Publications
Tipo: Artigo de Revista Científica
Publicado em //2011 EN
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Upper urinary tract (UUT) transitional cell carcinoma (TCC) is relatively rare tumor. Approximately 0.7-4% of patients with primary bladder cancer develops UUT-TCC. The symptoms related to an UUT-TCC often occur with an advanced stage which leads one to emphasize a surveillance strategy to monitor the UUT to allow for an earlier diagnosis. Although the risk of UUT-TCC after bladder cancer is well established, there is a paucity of recommendations suggesting the optimal method and frequency of monitoring the UUT and there is no consensus among them. This article reviews the recommendations on monitoring the UUT in patients with bladder cancer.

Competitive Selection from Single Domain Antibody Libraries Allows Isolation of High-Affinity Antihapten Antibodies That Are Not Favored in the llama Immune Response

Rosa, Sofia Tabares-da; Rossotti, Martin; Carleiza, Carmen; Carrión, Federico; Pritsch, Otto; Ahn, Ki Chang; Last, Jerold A.; Hammock, Bruce D; González-Sapienza, Gualberto
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
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Single-domain antibodies (sdAbs) found in camelids, lack a light chain and their antigen-binding site sits completely in the heavy-chain variable domain (VHH). Their simplicity, thermostability, and ease in expression have made VHHs highly attractive. While this has been successfully exploited for macromolecular antigens, their application to the detection of small molecules is still limited to a very few reports, mostly describing low affinity VHHs. Using triclocarban (TCC) as a model hapten, we found that conventional antibodies, IgG1 fraction, reacted with free TCC with a higher relative affinity (IC50 51.0 ng/mL) than did the sdAbs (IgG2 and IgG3, 497 and 370 ng/mL, respectively). A VHH library was prepared, and by elution of phage with limiting concentrations of TCC and competitive selection of binders, we were able to isolate high-affinity clones, KD 0.98–1.37 nM (SPR) which allowed development of a competitive assay for TCC with an IC50 = 3.5 ng/mL (11 nM). This represents a 100-fold improvement with regard to the performance of the sdAb serum fraction, and it is 100-fold better than the IC50 attained with other anti-hapten VHHs reported thus far. Despite the modest overall anti-hapten sdAbs response in llamas, a small subpopulation of high affinity VHHs are generated that can be isolated by carefully design of the selection process.

Effects of bisphenol A and triclocarban on brain-specific expression of aromatase in early zebrafish embryos

Chung, Eunah; Genco, Maria C.; Megrelis, Laura; Ruderman, Joan V.
Fonte: National Academy of Sciences Publicador: National Academy of Sciences
Tipo: Artigo de Revista Científica
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Estrogen regulates numerous developmental and physiological processes. Most effects are mediated by estrogen receptors (ERs), which function as ligand-regulated transcription factors. Estrogen also regulates the activity of GPR30, a membrane-associated G protein-coupled receptor. Many different types of environmental contaminants can activate ERs; some can bind GPR30 as well. There is growing concern that exposure to some of these compounds, termed xenoestrogens, is interfering with the behavior and reproductive potential of numerous wildlife species, as well as affecting human health. Here, we investigated how two common, environmentally pervasive chemicals affect the in vivo expression of a known estrogen target gene in the brain of developing zebrafish embryos, aromatase AroB, which converts androgens to estrogens. We confirm that, like estrogen, the well-studied xenoestrogen bisphenol A (BPA, a plastics monomer), induces strong brain-specific overexpression of aromatase. Experiments using ER- and GPR30-selective modulators argue that this induction is largely through nuclear ERs. BPA induces dramatic overexpression of AroB RNA in the same subregions of the developing brain as estrogen. The antibacterial triclocarban (TCC) by itself stimulates AroB expression only slightly...